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Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes
Duarte, Ana Rodrigues;Duarte, Débora Maria Rodrigues;Moreira, Keila Aparecida;Cavalcanti, Maria Taciana Holanda;Lima-Filho, José Luiz de;Porto, Ana Lúcia Figueiredo;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000100001
Abstract: the partial characterization and purification of milk clotting enzyme obtained from the (root latex) of jacaratia corumbensis o. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. the ammonium sulphate precipitate showed five fractions (as1- 0-20%; as2 - 20-40%; as3 - 40-60%; as4 - 60-80%; as5 - 80-100%) and among the fractions obtained, the 40-60% fraction (as3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. this fraction when applied on mono q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. the optimal ph for the crude and partially purified extract was 6.5 and 7.0, respectively. the maximum milk-clotting activity was at 55oc for the both crude and partially purified extracts. the enzyme was inhibited by iodoacetic acid which suggested that this enzyme was a cysteine protease, with molecular weight of 33 kda.
Estudo morfo-anat?mico de folhas de Jacaratia spinosa (Aubl.) A.DC. (Caricaceae)
Paoli, Adelita A. Sartori;Pagano, Sérgio Nereu;
Acta Botanica Brasilica , 1988, DOI: 10.1590/S0102-33061988000300019
Abstract: morphological and anatomical aspects of leaves of jacaratia spinosa (aubl.) a.dc. (caricaceae) were studied in detail. the leaf blade shows a dorsiventral organization, anomocytic stomata, glands, laticifers of the articulated anastomosed type and a papillary abaxial epidermis. the venation is brochidodromous ending in rings at the leaf margins.
Biologia da poliniza??o de Jacaratia spinosa (AUBL) ADC. (CARICACEAE) em mata residual do sudeste brasileiro
PIRATELLI, A. J.;PI?A-RODRIGUES, F. C. M.;GANDARA, F. B.;SANTOS, E. M. G.;COSTA, L. G. S.;
Revista Brasileira de Biologia , 1998, DOI: 10.1590/S0034-71081998000400015
Abstract: the pollination biology of jacaratia spinosa (aubl) adc. (caricaceae) was studied in mata de santa genebra, campinas municipality, s?o paulo state, brazil (20o54's; 47o05'w). data from floral anthesis, nectar volume and sugar concentration, pollen viability, stigma receptivity, location of scent glands, reproduction systems and main visitors/potential pollinators were obtained. the species is dioicious and the masculine plants flourish for a larger period (52 days) than the feminine ones (22 days). most of the flowers has night anthesis, lasting the masculine ones about 24 hours and the feminine ones, 48 hours. the masculine ones produce on the average, 112,5 ml of nectar and sugar concentration of 13,9%. the feminine ones didn't produce nectar, having been identified mimetism of the masculine ones by the feminine ones. the pollen viability didn't vary along the day (average 97,20%). there was production of fruits for agamospermy and for crossed fecundation. the main observed pollinators was moths, what corroborates with its characteristics of phalenophily syndrome; other insects meantime, as butterflies, also can contribute to its reproduction.
Biologia da poliniza o de Jacaratia spinosa (AUBL) ADC. (CARICACEAE) em mata residual do sudeste brasileiro
PIRATELLI A. J.,PI?A-RODRIGUES F. C. M.,GANDARA F. B.,SANTOS E. M. G.
Revista Brasileira de Biologia , 1998,
Abstract: A biologia da poliniza o de Jacaratia spinosa (Aubl) ADC. (Caricaceae) foi estudada na Mata de Santa Genebra, Campinas (20o54'S; 47o05'W), estado de S o Paulo, Brasil. Foram obtidos dados de antese floral, volume e concentra o de a úcar no néctar, viabilidade do pólen, receptividade do estigma, localiza o de glandulas de odor, sistemas de reprodu o e principais visitantes/potenciais polinizadores. A espécie é dióica e as plantas masculinas florescem por um período maior (52 dias) que as femininas (22 dias). A maioria das flores tem antese noturna, durando as masculinas cerca de 24 horas e as femininas, 48 horas. As masculinas produzem, em média, 112,5 ml de néctar e concentra o de a úcar de 13,9%. As femininas n o produzem néctar, tendo sido identificado mimetismo das masculinas pelas femininas. A viabilidade do pólen n o variou ao longo do dia (média 97,20%). Houve produ o de frutos por agamospermia e por fecunda o cruzada. Os principais polinizadores observados foram mariposas, o que corrobora com suas características de síndrome de falenofilia, havendo, entretanto, outros insetos, como borboletas, que podem contribuir para sua reprodu o.
As borboletas (Lepidoptera: Papilionoidea e Hesperioidea) da área de Prote??o Ambiental do Gama e Cabe?a de Veado (Distrito Federal, Brasil)
Pinheiro, Carlos Eduardo Guimar?es;Emery, Eduardo de Oliveira;
Biota Neotropica , 2006, DOI: 10.1590/S1676-06032006000300012
Abstract: a list containing 507 species of butterflies (papilionoidea e hesperioidea) found in the apa do gama e cabe?a de veado - df in the last four decades is presented in this study. the list includes data obtained in the literature, our personal collections, and entomological collections recently visited, but not species with presumed distribution in the region. the purpose of this study is (1) to demonstrate the importance of this apa for the conservation of the cerrado butterfly fauna and, (2) allow for monitoring such biodiversity in future studies.
Estatus micorrízico de Jacaratia mexicana y hongos formadores de micorriza arbuscular presentes en selvas bajas caducifolias del Golfo de México Mycorrhizal status of Jacaratia mexicana and presence of arbuscular mycorrhizal fungi in dry deciduous forests of the Gulf of Mexico  [cached]
Ramón Zulueta Rodríguez,Lucía Varela,Sergio Aguilar Espinosa,Dora Trejo Aguilar
Revista mexicana de micología , 2010,
Abstract: Se evaluó el estatus micorrízico de Jacaratia mexicana A. DC. y la presencia de morfoespecies de hongos micorrízico arbusculares en tres manchones de Selva Baja Caducifolia ubicados en la vertiente del Golfo de México. En ninguno de los materiales recolectados en campo se encontraron estructuras micorrízicas, y por ello se determinó la susceptibilidad de esta caricácea a la colonización radical en invernadero. La formación de hifas, arbúsculos y vesículas fue evidente a los 15 días después de la inoculación. Por otro lado, se identificaron seis morfoespecies: Glomus intraradices, G. sinuosum, G. constrictum, Glomus sp. 1, Glomus sp. 2 y Entrophospora infrequens. Las dos primeras no sólo fueron las únicas morfoespecies afines en los sitios muestreados (G. intraradices y G. sinuosum), sino también las más abundantes en Veracruz (8, 870 y 5), Campeche (958 y 448) y Yucatán (3, 708 y 16). Todas ellas se reportan por vez primera asociadas a J. mexicana en nuestro país. We evaluated the mycorrhizal status of Jacaratia mexicana A. DC. and the presence of arbuscular mycorrhizal fungi (AMF) in three patches of dry deciduous forests located on the slope of the Gulf of Mexico. There were no mycorrhizal structures in any of the materials collected in field, and therefore the susceptibility of this specie to AMF was determined. The study revealed the presence of arbuscles and vesicles 15 days after inoculation. Six morphospecies were identified: Glomus intraradices, G. constrictum,G. sinuosum, Glomus sp. 1, Glomus sp. 2. and Entrophospora infrequens. The only related morphospecies in sampled sites were G. intraradices and G. sinuosum, and they were also fairly abundant in Veracruz (8, 870 and 5), Campeche (958 and 448) and Yucatán (3, 708 and 16). All of them have not been reported associated with J. mexicana in our country before.
Genetic diversity and structure of wild populations of the tropical dry forest tree Jacaratia Mexicana (Brassicales: Caricaceae) at a local scale in Mexico
Dulce M. Arias,Ana L. Albarrán-Lara,Antonio González-Rodríguez,Juan Pe?aloza-Ramírez
Revista de Biología Tropical , 2012,
Abstract: The tropical dry forest is a greatly endangered ecosystem, from which Jacaratia mexicana is a native tree. With the aim to assess the levels of genetic variation and population structure, four wild populations of J. mexicana were studied in the Sierra de Huautla Biosphere Reserve, Morelos, Mexico. For this, DNA was extracted from 159 individuals and were amplified with six random primers using the Random Amplified Polymorphic DNA (RAPD). A total of 54 bands were obtained, of which 50 (92.6%) were polymorphic. The total genetic diversity found within the four populations was 0.451 when estimated by Shannon’s index. An AMOVA analysis showed that 84% of the total genetic variation was found within populations and 16% was among populations. The UPGMA dendrogram showed that all individuals from one of the populations (Huaxtla) formed one distinct genetic group, while the rest of the individuals did not cluster according to population. A Mantel test did not show an association between genetic and geographical distances among populations (r=0.893, p=0.20). A Bayesian cluster analysis performed with STRUCTURE, showed that the most probable number of genetic groups in the data was four (K=4), and confirmed the distinctness of Huaxtla population. Our results showed that important genetic differentiation among populations can occur even at this small geographic scale and this has to be considered in conservation actions for this genetic resource. Jacaratia mexicana es un árbol nativo del bosque tropical seco, que es considerado el tipo de vegetación en mayor riesgo de desaparecer completamente. Se utilizaron polimorfismos de ADN amplificados al azar (RAPD, Random Amplified Polymorphic DNA), para evaluar los niveles de variación y estructura genética en cuatro poblaciones silvestres de J. mexicana en la Reserva de la Biósfera Sierra de Huautla (Morelos, México). Se amplificó el ADN de 159 individuos utilizando seis oligonucleótidos (“primers”) aleatorios. Se obtuvieron en total 54 bandas, de las cuales 50 (92.6%) fueron polimórficas. La diversidad genética total que se encontró en las cuatro poblaciones de J. mexicana fue de 0.451 de acuerdo con el índice de Shannon. Un análisis de varianza molecular (AMOVA) mostró que el 84% de la variación genética total se encuentra dentro de las poblaciones y el 16% entre las poblaciones. Un dendrograma construido mediante el algoritmo UPGMA mostró que los individuos de una población (Huaxtla) formaron un grupo, mientras que el resto de los individuos no se agruparon de acuerdo a su población de origen. Una prueba de Mantel no
Estatus micorrízico de Jacaratia mexicana y hongos formadores de micorriza arbuscular presentes en selvas bajas caducifolias del Golfo de México
Zulueta Rodríguez, Ramón;Varela, Lucía;Aguilar Espinosa, Sergio;Trejo Aguilar, Dora;Lara Capistrán, Liliana;
Revista mexicana de micología , 2010,
Abstract: we evaluated the mycorrhizal status of jacaratia mexicana a. dc. and the presence of arbuscular mycorrhizal fungi (amf) in three patches of dry deciduous forests located on the slope of the gulf of mexico. there were no mycorrhizal structures in any of the materials collected in field, and therefore the susceptibility of this specie to amf was determined. the study revealed the presence of arbuscles and vesicles 15 days after inoculation. six morphospecies were identified: glomus intraradices, g. constrictum,g. sinuosum, glomus sp. 1, glomus sp. 2. and entrophospora infrequens. the only related morphospecies in sampled sites were g. intraradices and g. sinuosum, and they were also fairly abundant in veracruz (8, 870 and 5), campeche (958 and 448) and yucatán (3, 708 and 16). all of them have not been reported associated with j. mexicana in our country before.
Efeitos da sele??o para peso pós-desmame sobre medidas corporais e perímetro escrotal de machos Nelore de Sert?ozinho (SP)
Cyrillo, Joslaine Noely dos Santos Gon?alves;Razook, Alexander George;Figueiredo, Leopoldo Andrade de;Bonilha Neto, Luiz Martins;Ruggieri, Ana Cláudia;Tonhati, Humberto;
Revista Brasileira de Zootecnia , 2000, DOI: 10.1590/S1516-35982000000200013
Abstract: the objective of this study was to evaluate the indirect effects of selection for post-weaning weight on body measures and scrotal perimeter of 809 nellore males from selected herds (nes and net) and control herd (nec), of the esta??o experimental de zootecnia de sert?ozinho. the statistical analyses were performed by using a sire mixed model where the random source of variation, sires, was nested within herds. the fixed effects were herds, year of performance test (pgp), age of cow and age of the animal as a covariate. the average genetic change for final weight, corrected for 378 days of age (w378), calculated as a deviation from the nec herd, were 40.2 and 44.3 kg for the nes and net herds, respectively. the correlated changes, for the other traits were, in the same order, 4.5 and 4.5 cm for hip height (hh); 6.2 and 7.0 cm for chest girth (cg); 5.8 and 6.3 cm for body length (bl); 2.9 and 2.0 cm for dorsal line length (dl); 1.7 and 2.4 cm for rump length (rl); 1.0 and 1.3 cm for distance between pin bones (dpb); 1.8 and 2.6 cm for distance between hip bones (dhp); and 1.3 and 2.2 cm for scrotal perimeter (sp). the results of this study showed that the direct selection for postweaning weight promoted correlated positive responses in the body dimensions and also in the scrotal perimeter of nellore males.
Estrutura??o de um programa de qualifica??o em gest?o da qualidade, seguran?a e saúde ocupacional: apresenta??o dos resultados de uma aplica??o piloto realizada no aglomerado metal-mecanico de Sert?ozinho - S?o Paulo
Vitoreli, Gislaine Aparecida;Carpinetti, Luiz Cesar R;Gerolamo, Mateus Cecílio;Sordan, Juliano Endrigo;Lima, Carlos Henrique Bertucci;
Gest?o & Produ??o , 2012, DOI: 10.1590/S0104-530X2012000400003
Abstract: this article aims to present and discuss the framework of a qualification program on quality, occupational health and safety management for small and medium sized enterprises. the proposed qualification program includes an integrated management system model, as well as a process for conducting periodic audits. action research was the methodological approach chosen to conduct the pilot application of the proposed qualification program. the pilot application was performed with a group of small and medium enterprises of the sert?ozinho's metal-mechanic cluster, located in the state of s?o paulo preliminary results indicate the possibility of operationalization of the proposal as well as its contributions to two key aspects: increase competitiveness of the companies in the cluster and increase competence of the cluster governance team to coordinate joint actions. nevertheless, there are great challenges to maintain the qualification program, and its maintenance is one of the most critical challenges faced by the governance team.
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