oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
The Comparison of efficacy of lornoxicam and paracetamol for preemptive analgesia in mice  [PDF]
Soner Oflazo?lu,Levent ?ahin,?mer Akta?,Mehrican ?ahin
Dicle Medical Journal , 2011,
Abstract: Objectives: Preemptive analgesia means that block the peripheral and central nociception as an analgesic regime apply before the painful stimulus. We aimed compared preemptive analgesic properties of lornoxicam with paracetamol on mice in this experimental study.Materials and methods: Experimental mice were divided into three groups. Before the formalin injection in the right front foot of mice, saline administered in first group, lornoxicam administered in second group and paracetamol in third group intraperitoneally. Responses to pain of mice were recorded after the procedure during the 60 min.Results: While appears to be lornoxicam more effective than the control group in the first 10 minutes, paracetamol provided more effective analgesia lornoxicam and control group within 11-60th min. and 0-60th min.Conclusion: Although lornoxicam provided significant benefit in acute phase, paracetamol was found more effective in tonic phase and total time in this study.
The effects of ethanol on paracetamol-induced oxidative stress in mice liver  [PDF]
Mladenovi? Du?an,Ninkovi? Milica,Vu?evi? Danijela,?oli? Miodrag
Journal of the Serbian Chemical Society , 2013, DOI: 10.2298/jsc120724127m
Abstract: The aim of our study was to investigate the effects of binge drinking on paracetamol induced oxidative stress in mice liver. Male Swiss mice were divided into groups: control; ethanol-treated group (E) in five subsequent doses of 2 g/kg by orogastric tube; paracetamol-treated group (P) in a dose of 300 mg/kg intraperitoneally; group that received paracetamol 12 hrs after the last dose of ethanol (PE). Blood and liver samples were collected for determination of oxidative stress parameters 6, 24 and 48 hrs after treatment. Prior binge drinking potentiated paracetamol-induced rise in liver malondialdehyde level 48 hours after treatment in comparison with P and E groups (17.14 ± 1.98 vs 13.14 ± 0.82 and 12.99 ± 1.18 μmol/L, p<0.01). Ethanol and paracetamol in combination induced a more pronounced decrease in liver GSH level than either of these substances alone at all time intervals (p<0.01). Total liver superoxide dismutase (SOD) activity was significantly lower in PE 48 hours after treatment in comparison with P and E groups (251.73 ± 80.63 vs 707.62 ± 179.92 and 1179.62 ± 147.94 U/mg prot., p<0.01). The lowest MnSOD activity in PE group was detected 48 hrs after treatment (86.52 ± 28.31; 41.13 ± 11.07 and 23.16 ± 5.18 U/mg prot. in P, E and PE groups, p<0.05, respectively). Prior binge ethanol drinking potentiates paracetamol-induced reduction of antioxidative capacity of hepatocytes due to GSH depletion and SOD activity reduction, simultaneously increasing lipid peroxidation caused by paracetamol. [Projekat Ministarstva nauke Republike Srbije, br. 175015]
Effect of Escherichia coli infection on the histopathology of albino mice visceral organs
Abin Biswas,R.Balaji,R.Balaji Raja,Kantha D.Arunachalam
International Journal of Engineering Science and Technology , 2010,
Abstract: Histopathology is very essential in assessing the nature and state of the tissues. It is used widely to examine the visceral organs in various diseases, infections and disorders. It provides the required insight and details about the possible malfunctioning of the disease or infection. Albino mice were taken and test group was infected withEscherichia coli. The control group was not infected with any linical pathogen. The histopathological examination was carried out to determine the effect of infection in the test group. Liver, spleen and kidney were the visceral organs which were used for the study. The size, shape and other morphological characteristics had markeddifference in case of infected mice when compared with control group mice. Histopathological analysis can be further extended in the case of other clinical pathogenic infections which could lead to nteresting results.
The effects of Moringa stenopetala on blood parameters and histopathology of liver and kidney in mice
D Ghebreselassie, Y Mekonnen, G Gebru, W Ergete, K Huruy
Ethiopian Journal of Health Development , 2011,
Abstract: Background: Moringa stenopetala and related species are commonly used in folk medicine for various human diseases such as antimalarial, antihypertensive, antidiabetic and as antispasmodic. Objective: The aim of the study is to evaluate the effects of aqueous extract of M. stenopetala on blood parameters, and histopathology of liver and kidney in experimental mice. Methods: Fresh leaves of M. stenopetala were collected from Arbaminch area, Southwest Ethiopia, in November 2005. The leaves were dried and extracted with water. Three month-old Swiss albino male mice, which were kept under uniform laboratory conditions, were randomly divided into four groups (one group of controls and three experimental). (The control group was orally given 0.5 ml of distilled water, and groups II, III and IV were given the aqueous leaf extract of M. stenopetala using intragastric tube to achieve the required doses of 600, 750 and 900 mg/kg body weight, respectively once a day at 24 hours intervals for six weeks and then sacrificed). Blood sample was collected from each mouse and examined for hematological and biochemical parameters. Liver and kidney were removed, stained and examined for histopathological profiles. The effects of treatment with aqueous extract of M. stenopetala on hematological, biochemical and histopathology features were compared with control group following standard procedures. Results: Mice treated with 900 mg/kg of the extract per kg of body weight showed a significant increase in body weight compared to the controls (P=0.014). Neither a significant change in the weight nor in histopathology of liver and kidney were observed in the animals treated with aqueous extract of M. stenopetala compared to those of the controls. Serum glucose level (P=0.034) and serum cholesterol level (P=0.016) decreased significantly after six weeks treatment. Conclusion: The aqueous leaf extract of M. stenopetala is shown to increase body weight and reduce serum glucose and cholesterol level in mice. This indicates nutritional and medicinal values, but we cannot yet recommend its therapeutic use before more and complete studies are done.
Paroxetine Augments while Naloxone Abolishes the Analgesic Effect of Paracetamol in Acute Nociceptive Pain in Mice  [PDF]
Mohammad Raafat Abdalla, Waleed Al Malki, Muhammad Ahmed
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.44057
Abstract:

The mechanism(s) of analgesic action of paracetamol (acetaminophen; N-acetyl-p-aminophenol) remains controversial. Previous studies on rats suggested that the antinociceptive action of paracetamol might involve the central descending inhibitory pain pathways recruiting both a serotoninergic and an opioidergic system. This study explores this issue in mice using paroxetine, the most potent selective serotonin re-uptake inhibitor, and the nonselective opioid pure antagonist naloxone. Animals were divided into two main groups for two separate experiments, each subdivided into 3 subgroups. In both experiments; the first group served as control, the second group received paracetamol (200 mg/kg, i.p). In one experiment, the third group received paroxetine (20 mg/kg p.o for 7 days) before paracetamol. In the other experiment, animals of the third group were pretreated with naloxone (5 mg/kg, i.p) 30 min before paracetamol. The antinociceptive effect of paracetamol was tested using the hot plate test. Paracetamol displayed a significant antinociceptive activity that was augmented by pretreatment with paroxetine as was shown by maintenance of its effect beyond that shown by paracetamol alone. On the other hand, pretreatment with naloxone abolished paracetamol’s antinociceptive activity in the hot-plate test. These results extended the previous observation in rats that the antinociceptive effect of paracetamol involved activation of a central descending pain inhibitory pathway with serotonin and opioidergic peptides being potential mediators recruited.

Mycoplasma gallisepticum infection in the grey partridge Perdix perdix: outbreak description, histopathology, biochemistry and antioxidant parameters
Frantisek Vitula, Lucie Peckova, Hana Bandouchova, Miroslav Pohanka, Ladislav Novotny, David Jira, Jiri Kral, Karel Ondracek, Jitka Osickova, Dagmar Zendulkova, Katerina Rosenbergova, Frantisek Treml, Jiri Pikula
BMC Veterinary Research , 2011, DOI: 10.1186/1746-6148-7-34
Abstract: Morbidity and mortality rates were 100% and 60%, respectively. Necropsy revealed an accumulation of caseous exudate within the infraorbital sinuses, tracheitis, pneumonia and airsacculitis. There were significant increases in activities of lactate dehydrogenase, creatine kinase and amylase, and levels of total protein and glucose in Mycoplasma-infected birds when compared to control. Catalase showed significantly lower activity in the heart, lungs, liver and gonads of Mycoplasma-infected birds. Glutathione-S-transferase activity was elevated in the eye and the associated infraorbital sinus and kidneys, and decreased in the liver. Decreased levels of reduced glutathione were found in the heart, kidneys, liver and gonads. The activity of glutathione reductase was lower only in the lungs. Compared to healthy birds, mycoplasmosis in the grey partridge caused significant differences in the level of lipid peroxidation in lungs and plasma (p < 0.05), while the ferric reducing antioxidant power was lower in the heart and kidneys (p < 0.01). Significant correlations among responses of the antioxidant parameters were found namely in the heart, lungs, spleen, liver and plasma. There were also numerous significant inter-tissue correlations of all the studied antioxidant parameters.The present study demonstrates the high susceptibility of grey partridges to natural infection by M. gallisepticum, the severity of the disease based on histopathology, and the modulation of blood chemical profiles and oxidative stress-associated parameters in the avian hosts, thus enhancing the understanding of the pathogenesis of mycoplasmosis in birds. Moreover, the reported reference values can be useful for the evaluation of the state of health in grey partridges.The grey partridge Perdix perdix, a famous bird native to Europe and introduced to many parts of the world, has shown a marked population decline throughout Europe since the second half of the 20th century [1]. Factors driving this decli
Histopathology and Pathogenesis of Listeriosis Caused by Listeria monocytogenes Isolated from Raw Milk in Mice  [PDF]
H. Moshtaghi,M. Arabi,I. Karimi
Pakistan Journal of Biological Sciences , 2006,
Abstract: Pathogenicity and histopathology of listeria monocytogene isolates (11) from raw milk in mice was done. Swiss albino mice (18-22 g) were used as experimental animals. All the isolates were pathogenic and caused mortality within 2 to 6 days after intraperitoneal injection. Reisolation of the microorganism from different organs was done. Pathological changes in the liver, spleen, brain, kidneys and intestines were recorded.
Assessment of hepatoprotective effect of Tecomella undulata (Sm.) Seem., Bignoniaceae, on paracetamol-induced hepatotoxicity in rats
Patel, Krishna N.;Gupta, Gajendra;Goyal, Manoj;Nagori, B. P.;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000020
Abstract: the aim of the present study was to validate the hepatoprotective activity of bark of tecomella undulata (sm.) seem., biognoniaceae, against paracetamol (pcm) induced hepatic damage. chloroform soluble fraction (fraction-i), acetone soluble fraction (fraction-ii), methanol soluble fraction (fraction-iii) and methanol insoluble fraction (fraction-iv) of ethanolic extract of bark of t. undulata were evaluated for hepatoprotective activity against paracetamol induced hepatic damage using biochemical, morphological, functional and histopathological studies. the methanol soluble fraction (fraction-iii) was most potent among the four fractions studied in detail. fraction-iii showed significant hepatoprotective activity against paracetamol induced hepatic damage as evident by normalization of substantially elevated levels of aspartate amino transferase (ast), alanine amino transferase (alt), alkaline phosphatase (alp) and total bilirubin (tbil), decreased level of total protein (tp), increased wet liver weight and volume, increased thiopentone sodium induced sleeping time and abnormal histopathology. present study showed that the fraction-iii of ethanolic extract of bark of t. undulata significantly restores physiological integrity of hepatocytes. fraction-iii did not show any sign of toxicity up to oral dose of 1500 mg/kg in mice.
Assessment of hepatoprotective effect of Tecomella undulata (Sm.) Seem., Bignoniaceae, on paracetamol-induced hepatotoxicity in rats  [cached]
Krishna N. Patel,Gajendra Gupta,Manoj Goyal,B. P. Nagori
Revista Brasileira de Farmacognosia , 2011,
Abstract: The aim of the present study was to validate the hepatoprotective activity of bark of Tecomella undulata (Sm.) Seem., Biognoniaceae, against paracetamol (PCM) induced hepatic damage. Chloroform soluble fraction (Fraction-I), acetone soluble fraction (Fraction-II), methanol soluble fraction (Fraction-III) and methanol insoluble fraction (Fraction-IV) of ethanolic extract of bark of T. undulata were evaluated for hepatoprotective activity against paracetamol induced hepatic damage using biochemical, morphological, functional and histopathological studies. The methanol soluble fraction (Fraction-III) was most potent among the four fractions studied in detail. Fraction-III showed significant hepatoprotective activity against paracetamol induced hepatic damage as evident by normalization of substantially elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP) and total bilirubin (TBil), decreased level of total protein (TP), increased wet liver weight and volume, increased thiopentone sodium induced sleeping time and abnormal histopathology. Present study showed that the Fraction-III of ethanolic extract of bark of T. undulata significantly restores physiological integrity of hepatocytes. Fraction-III did not show any sign of toxicity up to oral dose of 1500 mg/kg in mice.
Hepatoprotective Potential of Clitoria ternatea Leaf Extract Against Paracetamol Induced Damage in Mice  [PDF]
Kuppan Nithianantham,Murugesan Shyamala,Yeng Chen,Lachimanan Yoga Latha,Subramanion L. Jothy,Sreenivasan Sasidharan
Molecules , 2011, DOI: 10.3390/molecules161210134
Abstract: Background and Aim: Clitoria ternatea, a medicinal herb native to tropical equatorial Asia, is commonly used in folk medicine to treat various diseases. The aim of the present study is to evaluate the hepatoprotective and antioxidant activity of C. ternatea against experimentally induced liver injury. Methods: The antioxidant property of methanolic extract (ME) of C. ternatea leaf was investigated by employing an established in vitro antioxidant assay. The hepatoprotective effect against paracetamol-induced liver toxicity in mice of ME of C. ternatea leaf was also studied. Activity was measured by monitoring the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and billirubin along with histopathological analysis. Results: The amount of total phenolics and flavonoids were estimated to be 358.99 ± 6.21 mg/g gallic acid equivalent and 123.75 ± 2.84 mg/g catechin equivalent, respectively. The antioxidant activity of C. ternatea leaf extract was 67.85% at a concentration of 1 mg/mL and was also concentration dependant, with an IC50 value of 420.00 μg/mL. The results of the paracetamol-induced liver toxicity experiments showed that mice treated with the ME of C. ternatea leaf (200 mg/kg) showed a significant decrease in ALT, AST, and bilirubin levels, which were all elevated in the paracetamol group (p < 0.01). C. ternatea leaf extract therapy also protective effects against histopathological alterations. Histological studies supported the biochemical findings and a maximum improvement in the histoarchitecture was seen. Conclusions: The current study confirmed the hepatoprotective effect of C. ternatea leaf extract against the model hepatotoxicant paracetamol. The hepatoprotective action is likely related to its potent antioxidative activity.
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.