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Allelic Polymorphism Identified and Analysis on the Fifth Exon of Chinese Indigenous Donkey GH Gene by PCR-SSCP
Wenjin Zhu,Yongmei Su,Yanfang Liu,Xuemin Guan,Jing Ni,Jianhua Wu
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.3713.3716
Abstract: Growth of animal is largely regulated by Growth Hormone (GH). In this study the objective was to investigate variations in the fifth exon of GH gene and their polymorphism in 5 Chinese indigenous donkeys by PCR-SSCP and DNA Sequencing Methods. The results showed that there was a transversion at nucleotide position 1802 of GH gene in the 5th exon named as GH-exon5-G1802C which led to a conservative Lysine to Asparagine substitution at amino acid position 205. In detail, two different alleles, A and B were identified and three genotypes were observed, AA, AB and BB with the frequency distribution of allele B from 0.5962-0.7344 in analyzed populations. The genetic diversity analysis revealed that all PIC values were between 0.3140 and 0.3656, implying that this locus within GH gene possessed moderate genetic diversity in 5 Chinese indigenous donkeys. The χ2-test showed that GZ donkey was significant deviation, DZ donkey was no significant deviation from Hardy-Weinberg equilibrium, GL, JN and XJ donkey was not in agreement Hardy-Weinberg equilibrium. The results confirmed that there were polymorphisms in the exon 5 of GH gene.
马生长激素(gh)基因的pcr-sscp研究  [PDF]
张焱如,芒来
牲畜兽医学报 , 2007,
Abstract: ?选取4个蒙古马地方品种、1个国内培育品种和1个国外引入品种共281匹马,采用pcr-sscp技术,检测了gh基因5'侧翼区、第1内含子、第2外显子和第5外显子4个位点的遗传多态性。结果显示:仅第5外显子出现多态性,表现出aa、bb和ab3种基因型,其余位点未检测到多态。群体遗传学分析结果表明:除纯血马外,其余品种都是等位基因a为优势基因;hardy-weinberg平衡检验显示,乌审马、乌珠穆沁马、巴尔虎马处于平衡状态,锡尼河马、三河马、纯血马处于非平衡状态;各品种马的多态信息含量均为中度多态(0.25
Single strand confirmation polymorphism (SSCP) detection in exon I of the a-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)
Kumar, Dinesh;Gupta, Neelam;Ahlawat, S.P.S.;Satyanarayana, R.;Sunder, Shyam;Gupta, S.C.;
Genetics and Molecular Biology , 2006, DOI: 10.1590/S1415-47572006000200016
Abstract: the genetic diversity of jamunapari goats (capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (pcr-sscp) method to detect a-lactalbumin polymorphism in a sample of 50 goats. our data show that pcr-sscp is an appropriate tool for evaluating genetic variability in jamunapari goats. polymorphism was detected in the sample, indicating that jamunapari goats have high genetic variability at loci, exon i of the a-lactalbumin gene. this result opens interesting prospects for future selection programs and conservation strategies. these a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (snp) markers for association studies and marker assisted selection.
Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats
Deng, Chanjuan;Ma, Rongnuan;Yue, Xiangpeng;Lan, Xianyong;Chen, Hong;Lei, Chuzhao;
Genetics and Molecular Biology , 2010, DOI: 10.1590/S1415-47572010005000034
Abstract: the association of igf-i gene polymorphisms with certain traits in 708 individuals of two chinese dairy-goat breeds (guanzhong and xinong saanen) was investigated. polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) and dna sequencing methods were employed in screening for genetic variation. two novel mutations were detected in the 5'-flanking region and in intron 4 of igf-i gene, viz., g.1617 g > a and g.5752 g > c (accession d26119.2), respectively. the associations of the g.1617 g > a mutation with milk yield and the body size were not significant (p > 0.05). however, in the case of g.5752 g > c, xinong saanen dairy goats with the cg genotype presented longer bodies (p < 0.05). chest circumference (p < 0.05) was larger in guanzhong goats with the gg genotype. in xinong saanen dairy goats with the cc genotype, milk yields were significantly higher during the first and second lactations (p < 0.05). hence, the g.5752 g > c mutation could facilitate association analysis and serve as a genetic marker for chinese dairy-goat breeding and genetics.
Relationship Between Some Polymorphic Parameters and Performances in Damascus Goats  [PDF]
O. Guney,O. Ozuyanik,O. Torun,M. Gorgulu
Pakistan Journal of Biological Sciences , 2003,
Abstract: The objective of this study was to determine the types of haemoglobin and transferrin found in Damascus goats and demonstrate their relationship with different performance traits. The Hb B allele was observed in the Damascus goats studied. No significant deviation from Hardy-Weinberg equilibrium was observed. We observed nine genotypes, namely Tf AA, AC, AD, BB, BC, BD, CC, CD, DD; C and D were the two most commonly occuring alleles at the locus and A was the rare -globin variant. The frequencies of Tf C and D were higher than those of the other transferrin genotypes. There was no significant effect of transferrin genotype on performance (p>0.05).
PCR-SSCP Polymorphism of FSHβ Gene and Its Relationship with Prolificacy of Jining Grey Goats
FSHβ 基因PCR-SSCP多态性及其与济宁青山羊高繁殖力关系的研究

LIANG Chen,CHU Ming-Xing,ZHANG Jian-Hai,LIU Wen-Zhong,FANG Li,YE Su-Cheng,
梁 琛
,储明星,张建海,刘文忠,方 丽,叶素成

遗传 , 2006,
Abstract: The follicle-stimulating hormone beta-subunit (FSHbeta) gene was studied as a candidate gene for the prolificacy in Jining Grey goats. According to the sequence of ovine FSHb gene, nine pairs of primers were designed to detect single nucleotide polymorphisms of 5' regulatory region, exon 1 and exon 2 of FSHbeta gene in both high fecundity breed (Jining Grey goat) and low fecundity breeds (Liaoning Cashmere goat, Boer goat and Angora goat) by PCR-SSCP. The results indicated that the homology of nucleotide sequence of this fragment between goat and sheep was 98 percent. Only the products amplified by primer P9 displayed polymorphism. Three genotypes (AA, AB and AC) were detected in Jining Grey goats and Liaoning Cashmere goats. Three genotypes (AA, CC and AC) were detected in Boer goats. Six genotypes (AA, BB, CC, AB, AC and BC) were detected in Angora goats. Sequencing revealed a G-->A mutation at 94 bp of exon 2 of FSHbeta gene in the BB genotype in comparison to the AA genotype and a C-->T mutation at 174 bp of exon 2 of FSHbeta gene in the CC genotype in comparison to the AA genotype. The former mutation resulted in an amino acid change: alanine-->threonine, and the latter mutation did not cause any amino acid change. Genotype frequency of AA, AB and AC was 0.686, 0.137 and 0.177 in Jining Grey goats, respectively. The does with genotype AA had 0.78 (P<0.05) or 0.64 (P<0.05) kids more than those with genotype AB or AC in Jining Grey goats, respectively.
Structure and Reproductive Performances of Sahelian Goats in the Guera Region, in Central Chad  [PDF]
Logténé Youssouf Mopaté, Vounparet Zeuh, Issa Youssouf Adoum, Djimtoloum Nadjissara
Open Journal of Animal Sciences (OJAS) , 2014, DOI: 10.4236/ojas.2014.44022
Abstract: The purpose of the study was to establish the structure of monitored animal farms and determine reproductive parameters of Sahelian goats in the Guera Region, in Central Chad. Data were collected through longitudinal survey in 27 observatory farms during three years using goats identified by numbered ear tags. The herds were composed on average of about 71% of females. The age pyramid covering all years showed a significant number of males aged between 0 - 1 year and perceptible fall thereafter. However, some stability was observed in the class of the 2 - 8 years old among females. The frequencies of accumulated kiddings showed that 44% of births occurred in the rainy season against 28% respectively in the cool dry and hot dry seasons. Obtained reproduction parameters were: a sex ratio of 0.51 in favor of males, a kidding interval of 10.6 months with significant differences (P < 0.05) according to the rank of birth, an age at first kidding of 16.5 months for females born in monitoring and an age of 20.3 months for all first-kidding, giving an apparent fertility rate of 145%, a fecundity rate of 116% and an abortion rate of 2%. The composition and structure of herds have highlighted the importance of females to ensure the reproducibility of goat production systems. The determined reproduction parameters are references for actions to improve goat rearing in this area.
αS1-casein genetic variants in Sarda goat breed
G.M. Vacca,L. Chianese,A. Ghibellini,V. Carcangiu
Italian Journal of Animal Science , 2011, DOI: 10.4081/ijas.2003.s1.55
Abstract: Sardinia plays an important role in Italian goat breeding; this is due not only to the high extent of its patrimony and production, but also to the fact that the main breed, the Sarda goat, represents an interesting biodiversity source. As regards the morphologic variability, marked differences between subjects reared in the coast and mountain areas were found (Macciotta et al., 2002). These differences are due to the genetic selection on the autochthonous patrimony, set up by breeders and based on coat colour, horn presence and/or on functional criteria (udder shape). The need to improve milk yields also induced various breeders, mainly in those areas with better pasture conditions, to crossbreed the Sarda goats........
Effects of short-term feed restriction on milk yield and composition, and hormone and metabolite profiles in mid-lactation Sarda dairy sheep with different body condition score
Giuseppe Pulina,Anna Nudda,Gianni Battacone,Corrado Dimauro
Italian Journal of Animal Science , 2012, DOI: 10.4081/ijas.2012.e28
Abstract: Ten Sarda dairy ewes (5 with high Body Condition Score: H-BCS, BCS>2.5; BW 48.8±5.4 kg; 5 with low BCS: L-BCS, BCS<2.5; BW 36.2±4.7 kg) were subjected, after 7-day preliminary (Prel) period, to short-term feed restriction (FR, 50% of nutrient requirements) for three days followed by refeeding (Re-Fed, 100% requirements) for three days. Milk yield and composition (protein, fat, lactose, MUN, SCC, fatty acids), and blood parameters (glucose, NEFA, BUN, insulin, GH, IGF-I, leptin) were monitored. Milk yield decreased during FR in both BCS groups: at day 3 it was 38% and 35% of Prel values in HBCS and L-BCS ewes, respectively, reaching Prel levels at Re-Fed in both groups. Milk fat concentration was influenced by BCS¥sampling, increasing in H-BCS ewes during FR, but not varying in L-BCS ewes throughout the trial. During FR, milk protein increased as milk yield decreased. There was no change in milk urea nitrogen concentration during FR, but this decreased in both BCS groups during Re-Fed. FR modified the FA profile of milk fat in both BCS groups, increasing LCFA at the expense of SCFA and MCFA. Some blood parameters (NEFA, GH and IGF-I) were influenced by BCS, whereas almost all parameters were influenced by sampling. There was a rapid return to initial levels in all parameters except milk urea, blood urea and insulin at Re-Fed.
Effects of short-term feed restriction on milk yield and composition, and hormone and metabolite profiles in mid-lactation Sarda dairy sheep with different body condition scor  [cached]
Giuseppe Pulina,Anna Nudda,Gianni Battacone,Corrado Dimauro
Italian Journal of Animal Science , 2012,
Abstract: Ten Sarda dairy ewes (5 with high Body Condition Score: H-BCS, BCS>2.5; BW 48.8±5.4 kg; 5 with low BCS: L-BCS, BCS<2.5; BW 36.2±4.7 kg) were subjected, after 7-day preliminary (Prel) period, to short-term feed restriction (FR, 50% of nutrient requirements) for three days followed by refeeding (Re-Fed, 100% requirements) for three days. Milk yield and composition (protein, fat, lactose, MUN, SCC, fatty acids), and blood parameters (glucose, NEFA, BUN, insulin, GH, IGF-I, leptin) were monitored. Milk yield decreased during FR in both BCS groups: at day 3 it was 38% and 35% of Prel values in HBCS and L-BCS ewes, respectively, reaching Prel levels at Re-Fed in both groups. Milk fat concentration was influenced by BCS¥sampling, increasing in H-BCS ewes during FR, but not varying in L-BCS ewes throughout the trial. During FR, milk protein increased as milk yield decreased. There was no change in milk urea nitrogen concentration during FR, but this decreased in both BCS groups during Re-Fed. FR modified the FA profile of milk fat in both BCS groups, increasing LCFA at the expense of SCFA and MCFA. Some blood parameters (NEFA, GH and IGF-I) were influenced by BCS, whereas almost all parameters were influenced by sampling. There was a rapid return to initial levels in all parameters except milk urea, blood urea and insulin at Re-Fed.
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