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APPLICATION OF SCAR MOLECULAR MARKER TECHNOLOGY IN IDENTIFICATION OF LENTINULA EDODES
SCAR分子标记技术在香菇菌株鉴定上的应用研究

WU Xue-Qian,LI Hai-Bo,WEI Hai-long,FU Li-zhong,WU Qing-Qi,PENG Hua-Zheng,ZHU Mu-Yuan,
吴学谦
,李海波,魏海龙,付立忠,吴庆其,彭华正,朱睦元

菌物学报 , 2005,
Abstract: This study was designed for the purpose of establishing an effective way for rapid identification of Lentinula edodes strains based on DNA molecular marker. The RAPD (Random Amplified Polymorphic DNA) analysis was conducted on 14 cultivated strains of Lentinula edodes. A specific RAPD marker with a length of 1166kb, XG1166, could be generated from the Lentinula edodes strain 162. Subsequently, the RAPD marker was converted into more stable SCAR(Sequence Characterized Amplified Region) marker by the use of molecular cloning technology. In the same way, another SCAR marker with a length of 347kb, SX347, was obtained from Lentinula edodes strain Shenxiang-10. The results showed SCAR marker of strain 162 or Shenxiang-10 acquired based on this study could be used as a specific DNA fingerprint to identify strain 162 or Shenxiang-10 whithin one day. Consequently, it was suggested that SCAR molecular marker technology is an effective new way to identify Lentinula edodes strains more accurately and rapidly and it could be applied widely in the protection of germplasm resources, classification and identification distinguishing false strains of edible mushroom.
Use of ITS and ISSR markers to identify cultivated strains for Lentinula edodes
用ITS和ISSR分子标记技术鉴别香菇生产用种

QIN Lian-Hua,SONG Chun-Yan,TAN Qi,CHEN Ming-Jie,PAN Ying-Jie,
秦莲花
,宋春艳,谭琦,陈明杰,潘迎捷

菌物学报 , 2006,
Abstract: 16 samples were selected including 12 cultivated strains with similar or confused names for Lentinula edodes, 3 strains for Lentinus tigrinus, and one strain for Lentinus lepideus in this study. These samples were firstly analyzed at the level of species by ITS technique, and then were analyzed by ISSR technique. The sizes of the fragments from 3 species amplified by ITS technique were different, which can be used to identify strains from different species. Two fragments owned to Lentinula edodes strains were obtained and 5 pairs of strains for Lentinula edodes showed the same band patterns each other while strains from different species had different band patterns by ISSR analysis. The results were proved by RAPD analysis. In a conclusion, ITS and ISSR techniques can be used to identify cultivated strains for Lentinula edodes.
In Vitro Assessment of Shiitake Mushroom (Lentinula edodes) Extract for Its Antigingivitis Activity
Lena Ciric,Anna Tymon,Egija Zaura,Peter Lingstr m,Monica Stauder,Adele Papetti,Caterina Signoretto,Jonathan Pratten,Michael Wilson,David Spratt
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/507908
Abstract: Gingivitis is a preventable disease characterised by inflammation of the gums due to the buildup of a microbial biofilm at the gingival margin. It is implicated as a precursor to periodontitis, a much more serious problem which includes associated bone loss. Unfortunately, due to poor oral hygiene among the general population, gingivitis is prevalent and results in high treatment costs. Consequently, the option of treating gingivitis using functional foods, which promote oral health, is an attractive one. Medicinal mushrooms, including shiitake, have long been known for their immune system boosting as well as antimicrobial effects; however, they have not been employed in the treatment of oral disease. In the current study, the effectiveness of shiitake mushroom extract was compared to that of the active component in the leading gingivitis mouthwash, containing chlorhexidine, in an artificial mouth model (constant depth film fermenter). The total bacterial numbers as well as numbers of eight key taxa in the oral community were investigated over time using multiplex qPCR. The results indicated that shiitake mushroom extract lowered the numbers of some pathogenic taxa without affecting the taxa associated with health, unlike chlorhexidine which has a limited effect on all taxa.
Characterization of a mycovirus associated with the brown discoloration of edible mushroom, Flammulina velutipes
Yumi Magae, Masahide Sunagawa
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-342
Abstract: Purified FvBV particles contained two dsRNA genomes (dsRNA1 and 2). The complete sequence of dsRNA1 was 1,915 bp long, containing a single open reading frame (ORF) that encoded 580 amino acids of a putative 66-kDa RNA-dependent RNA polymerase (RdRp). dsRNA2 was 1,730 bp long containing a single ORF encoding 541 amino acids of a putative 60-kDa coat protein (CP1). Phylogenetic analysis of the RdRp sequences revealed FvBV to be a Partitivirus, most closely related to Chondrostereum purpureum cryptic virus. An RT-PCR assay was developed for the amplification of a 495-bp cDNA fragment from dsRNA encoding the CP1. When wild F. velutipes isolated from various parts of Japan were examined by RT-PCR assay, three isolates from the central region of Japan contained FvBV. One wild strain infected with FvBV was isolated in Nagano prefecture, where brown discoloration of white cultivated strains has occurred. Fruiting bodies produced by virus-harboring and virus-free F. velutipes were compared.Cap color of the fruiting bodies of F. velutipes that contained Partitivirus FvBV was darker than FvBV-free fruiting bodies. The use of RT-PCR enabled association of FvBV and dark brown color of the fruiting body produced by F. velutipes strains.At the time mycoviruses were discovered in the white button mushroom, Agaricus bisporus, in 1962 [1], Lentinula edodes was the only artificially cultivated mushroom in Japan. Since then, the number of cultivated mushroom species has greatly increased: L. edodes, Flammulina velutipes, Hypsizygus marmoreus, Pholiota nameko, Grifola frondosa, and Pleurotus eryngii are cultivated daily. As the mushroom industry in Japan continues to grow, various abnormal symptoms during cultivation have been observed. Symptom that resembled abiotic disorders of A. bisporus [2] first became apparent with cultivated strains of F. velutipes [3,4]. One of the abnormal symptoms was the spontaneous appearance of brown discolored fruiting bodies among the white ones. We dete
Insects associated with edible mushroom Lentinula edodes (Berk.) Pegler (Agaricales: Agaricaceae) cultivation in Brazil Insetos associados ao cultivo do cogumelo comestível Lentinula edodes (Berk.) Pegler (Agaricales: Agaricaceae) no Brasil  [cached]
Marliton R. Barreto,Noemia Kazue Ishikawa,Ulisses B. Albino,Gisele M. Andrade Nobrega
Semina : Ciências Biológicas e da Saúde , 2002,
Abstract: Lentinula edodes has adapted itself well to the Brazilian climate and its cultivation techniques are accessible to small and large producers. As with any other crop, the shiitake faces insect and pest attack problems, both during the mycelia colonization period in the substrate and during the basidiocarp formation. There is no report up to now of the pests that may attack in the crop in Brazil. Consequently, this study was carried out as a preliminary survey of the insects associated with shiitake cultivation both in the growing substrate and in the fruiting bodies. The samples were taken from properties situated in Londrina (PR), Rolandia (PR) Nova Friburgo (RJ) Sorocaba (SP) and Vi osa (MG). As a result, insects from the orders Coleoptera (Bostrichidae, Staphilinidae and Dermestidae), Diptera (Calliphoridae), Himenoptera (Formicidae) and Lepidoptera (Stenomatidae), were found. O cultivo de Lentinula edodes apresenta-se em crescente expans o no Brasil. Um dos problemas enfrentados pelos produtores é o ataque de insetos, tanto no substrato de crescimento do micélio, como durante o processo de forma o do basidiocarpo. Este trabalho teve por objetivo fazer um levantamento preliminar dos insetos associados ao cultivo do shiitake. Deste modo, foram realizadas coletas de insetos invasores de culturas, por meio do uso de pu á e sugadores, em Londrina (PR), Rolandia (PR), Nova Friburgo (RJ), Sorocaba (SP) e Vi osa (MG). Como resultado, foram encontrados insetos das ordens Coleóptera (Bostrichidae, Staphilinidae e Dermestidae), Diptera (Calliphoridae), Himenoptera (Formicidae) e Lepidoptera (Stenomatidae).
Mapping of a SCAR marker tightly linked to the B mating type locus of Lentinula edodes (shiitake mushroom) by tetrad analysis  [cached]
K. Miyazaki,A. Tanaka,S. Shiraishi
Micología Aplicada Internacional , 2008,
Abstract: We isolated 132 basidiosporic strains of 33 tetrads from the outbred strains MCR14 and MCR15 of Lentinula edodes for linkage analyses. Mating tests detected crossing over between genes of the B mating-type locus during meiosis in four strains, MCR14B-121-3 (B3), MCR14B-121-4 (B4), MCR14B-130-1 (B3), and MCR14B-130-4 (B4), as monokaryons having different mating types from the parental type (B1 , B2). In MCR14B-121-3 and MCR14B-121-4, the recombination was also detected between a sequence characterized amplified region (SCAR) marker, sOPP19-560, tightly linked to the B mating-type locus and a random amplified polymorphic DNA (RAPD) marker, C02-0900t. Furthermore, sOPP19-560 mapped between the B mating-type locus and a RAPD marker, Y07-0820t
A Review on General Nutritional Compounds and Pharmacological Properties of the Lentinula edodes Mushroom  [PDF]
Tiane Cristine Finimundy, Aldo José Pinheiro Dillon, Jo?o Ant?nio Pêgas Henriques, Mariana Roesch Ely
Food and Nutrition Sciences (FNS) , 2014, DOI: 10.4236/fns.2014.512119
Abstract:

Lentinula edodes is a macrofungus with great potential for therapeutic applications and serves as a model for investigating functional fungi properties and isolating pure compounds for pharmaceutical use. Mushrooms have a great nutritional value and present medicinal molecules including polysaccharides, terpenoids, sterols and lipids, that participate actively in several human disorders and modulate mechanisms involved in the immune system regulation. This review will focus on general nutritional compounds and pharmacological properties of L. edodes.

ANTIBACTERIAL ACTIVITY OF LENTINULA EDODES GROWN IN LIQUID MEDIUM
Ishikawa, Noemia Kazue;Kasuya, Maria Catarina Megumi;Vanetti, Maria Cristina Dantas;
Brazilian Journal of Microbiology , 2001, DOI: 10.1590/S1517-83822001000300008
Abstract: the antibacterial activity of 35 isolates of lentinula edodes, a shiitake mushroom, against bacillus subtilis was evaluated by diffusion technique in agar with a semi-solid overlay. all isolates inhibited b. subtilis and the isolate le1 promoted the formation of the largest inhibition zone. l. edodes le1 also presented antibacterial activity against foodborne pathogens and food contaminant bacteria, particularly gram-positive species. the antibacterial activity of the culture filtrate after 18-25 days of cultivation of l. edodes in broth at 25oc was high. the inhibitory activity was observed only in the organic layer when the culture filtrate was partitioned between ethyl acetate and water, suggesting that the inhibitory substances have low polarity. the silica gel thin-layer zone at rf values of 0.63-0.80, developed in chloroform - acetone - ethyl acetate - methanol = 40:5:5:2, was responsible for the antibacterial activity against b. subtilis. the inhibitory activity of l. edodes was detectable in the culture filtrate after heat treatment at 100oc for 10 min and after storage at 4oc for 120 days.
ANTIBACTERIAL ACTIVITY OF LENTINULA EDODES GROWN IN LIQUID MEDIUM  [cached]
Ishikawa Noemia Kazue,Kasuya Maria Catarina Megumi,Vanetti Maria Cristina Dantas
Brazilian Journal of Microbiology , 2001,
Abstract: The antibacterial activity of 35 isolates of Lentinula edodes, a shiitake mushroom, against Bacillus subtilis was evaluated by diffusion technique in agar with a semi-solid overlay. All isolates inhibited B. subtilis and the isolate Le1 promoted the formation of the largest inhibition zone. L. edodes Le1 also presented antibacterial activity against foodborne pathogens and food contaminant bacteria, particularly Gram-positive species. The antibacterial activity of the culture filtrate after 18-25 days of cultivation of L. edodes in broth at 25oC was high. The inhibitory activity was observed only in the organic layer when the culture filtrate was partitioned between ethyl acetate and water, suggesting that the inhibitory substances have low polarity. The silica gel thin-layer zone at Rf values of 0.63-0.80, developed in chloroform - acetone - ethyl acetate - methanol = 40:5:5:2, was responsible for the antibacterial activity against B. subtilis. The inhibitory activity of L. edodes was detectable in the culture filtrate after heat treatment at 100oC for 10 min and after storage at 4oC for 120 days.
Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70
Ribeiro, Liliane Fraga Costa;Vaz, Marcelo Gomes Mar?al Vieira;Chaves-Alves, Virgínia Maria;Vanetti, Maria Cristina Dantas;Kasuya, Maria Catarina Megumi;Passos, Flávia Maria Lopes;Nascimento, Ant?nio Galv?o do;
Brazilian Journal of Microbiology , 2012, DOI: 10.1590/S1517-83822012000100021
Abstract: xylanolytic enzymes produced by lentinula edodes ufv70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65oc for 21 hours, losing only 15-25% activity. fungus incubation at 50oc for 12 hours and at 65oc for 24 hours increased the amount of xylose produced.
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