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DETERMINATION OF GALLIC ACID IN ACACIA NILOTICA LINN. BY HPTLC  [PDF]
V Leela *
International Journal of Pharmacy and Technology , 2010,
Abstract: The stem bark of Acacia nilotica is used as a powerful astringent and the decoction is used as gargle for throat troubles and stomatitis.q3 Bark contains several polyphenols such as catechin,epicatechin, dicatechin, quercetin and tannin. Gallic acid has been reported to have antidiabetic,antithrombotic, anti inflammatory and anticarcinogenic activities. A simple, precise and accuratehigh-performance thin layer chromatographic method has been established for the determination of Gallic acid in the bark powder of Acacia nilotica Linn. The acetone extract of the bark powder wasused for the experimental work. Separation was performed on Silica gel 60 F254 HPTLC plates with Toluene: Ethyl acetate: Formic acid (6:4:0.8 v/v), as mobile phase. The plate was scanned in the densitometric absorbance mode at 280 nm. Gallic acid response was linear over the range 2-7 μg ml-1. The HPTLC method was validated in terms of sensitivity, accuracy, precision and reproducibility. The concentration of gallic acid in the bark powder was found to be 0.86%.
QUANTIFICATION OF GALLIC ACID AND ELLAGIC ACID IN ARJUNARISHTA BY VALIDTAED HPTLC DENSITOMETRY  [PDF]
Preeti Tiwari* and Rakesh K. Patel
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: Arjunarishta, also known as Parthadhyarishta, is a polyherbal hydro alcoholic formulation and is advised as a choice of remedy in cardiovascular disorders. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Arjunarishta–T and Arjunarishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ, specificity, robustness and ruggedness. The amount of gallic acid in Arjunarishta-T, M and its marketed formulation was found to be 0.0332, 0.0331 and 0.0330% w/w respectively while ellagic acid was found to be 0.0361, 0.0360 and 0.0359% w/w respectively. This is the first report for the quantification of gallic acid and ellagic acid in Arjunarishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and ellagic acid from Arjunarishta.
Determination of gallic acid in Phyllanthus emblica Linn. dried fruit powder by HPTLC  [cached]
Sawant Laxman,Pandita Nancy,Prabhakar Bala
Journal of Pharmacy and Bioallied Sciences , 2010,
Abstract: Objective : Emblica (Phyllanthus emblica L.), an euphorbiaceous plant, is widely distributed in subtropical and tropical areas of India, China and Indonesia. The fruits possess antimicrobial, antioxidant, anti-inflammatory, analgesic and antipyretic properties. In the current article a new, simple, sensitive, selective, precise, and robust high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the determination of gallic acid in dried fruit powder of Phyllanthus emblica. Materials and Methods : The quantitative determination of gallic acid was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. The linear ascending development was carried out in a twin trough glass chamber saturated with a mobile phase consisting of toluene: ethyl acetate: formic acid: methanol (3:3:0.8:0.2) at room temperature (25 ± 2°C). Camag TLC scanner III was used for spectrodensitometric scanning and analysis, in the absorbance mode, at 278 nm. Results : The linear regression analysis data for the calibration plots showed good linear relationship with r 2 = 0.99977 in the concentration range of 40 - 240 ng spot 1, with respect to the peak area. According to the guidelines of the International Conference on Harmonization (ICH), the method was validated for precision, accuracy, and recovery. Conclusion : Statistical analysis of the data showed that the method was reproducible and selective for the estimation of gallic acid.
Evaluation of wound healing activity of Acacia leucophloea bark in rats  [cached]
Sembian Suriyamoorthy,Kalidass Subramaniam,Femina Wahab,G Karthikeyan
Revista Brasileira de Farmacognosia , 2012,
Abstract: Wound healing activity of the bark extracts of Acacia leucophloea Willd., Fabaceae, was investigated by excision and incision wound healing models in Wistar male rats. Ethanolic extract based ointment of A. leucophloea bark (2 and 5% (w/w)) was formulated and evaluated for its wound healing in Wistar male rats. In comparision with a standard wound healing ointment betadine. A. leucophloea ethanolic extract ointment exhibited marked wound healing activity and significantly enhanced the wound contraction and the period of epithelialization as assessed by wound contraction rate, tensile strength, increasing of DNA, collagen and protein synthesis and histopathological examination. The formulated ointment might well find use as skin repair agent without hazard to human health based on these results.
Quantitative Estimation of Gallic Acid as Biomarker in Lipitame Tablets by HPTLC Densitometry for Diabetic Dyslipidemia  [PDF]
Sheeraz Siddiqui, Khan Usmanghani, Aqib Zahoor, Zeeshan Ahmed Sheikh, Saleha Suleman Khan
Chinese Medicine (CM) , 2014, DOI: 10.4236/cm.2014.54021
Abstract: Lipitame is a poly herbal formulation comprised of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica. The formulation is investigated for its analysis evaluation. Biomarkers of three herbs such as Terminalia arjuna, Terminalia belerica, and Phyllanthus emblica have been already cited to contain gallic acid, which was qualitatively and quantitatively estimated. In the present study rapid and inexpensive qualification methods for the quality control of Terminalia arjuna, Terminalia belerica, Commiphora mukul and Phyllanthus emblica on thin layer chromatography (TLC) were developed and validated. The solvent system used was toluene:ethyl acetate:formic acid:methanol (12:9:4:0.5). The scanning of plate was performed linearly at 273 nm (absorption) by use of a TLC Scanner III CAMAG with a deuterium source, and the area of spots corresponding to Gallic acid standard was integrated. It was found that gallic acid has been found in the Lipitame tablets on HPTLC densitometry assessment compared with authentic gallic acid reference standard.
PROTEASE INHIBITORS OF ACACIA LEUCOPHLOEA GUM EXTRACTS  [cached]
Balaji M Panchal and Manvendra S Kachole
International Journal of Bioassays , 2012,
Abstract: Plant protease inhibitors (PIs) are very important for their defensive function against plant pathogens and predators. In present work trypsin and chymotrypsin inhibitors (PIs) from gum of one tree about seven species, Azadirachta indica, Acacia leucophloea, Acacia nilotica, Terminalia spp., Anogeissus latifolia, Mangifera indica and Moringa oleofera are studied. Protease inhibitory activity in gum extract was detected by dot blot assay. PI bands were resolved on electrophoresis gel and detected by Gel X-ray film contact print technique (GXCP). PIs from gum extracts were purified by gel filtration (Sephadex G-75). Among all gum extracts studied, the gum extract from Acacia leucophloea showed highest number of trypsin and chymotrypsin inhibitors. One PI from the Acacia leucophloea of 97.00kDa was purified and characterized. Purified PI was not destroyed by heat treatments up to 70oC, but lost its activity when incubated at 80°C, showing moderate thermo stability.
Occurrence of curcuminoids in Curcuma longa: A quality standardization by HPTLC
M. Paramasivam,Md. Wasim Aktar,R. Poi,H. Banerjee
Bangladesh Journal of Pharmacology , 2008,
Abstract: A simple high performance thin layer chromatographic (HPTLC) method has been developed for the simultaneous determination of the pharmacologically important active curcuminoids viz. curcumin, demethoxycurcumin and bis-demethoxycurcumin in Curcuma longa L. The assay combines the separation and quantification of the analytes on silica gel 60 GF254 HPTLC plates with visualization under UV and scanning at 425 nm. Using this technique, the alkaloidal content of different parts of the title plant has been determined.
A Comparative Evaluation of Phytochemical Fingerprints of Asteracantha longifolia Nees. Using HPTLC  [PDF]
S. Sunita,S. Abhishek
Asian Journal of Plant Sciences , 2008,
Abstract: Chromatographic techniques can be used to document phytochemical fingerprints and quantitate chemical markers to identify morphological and geographical variations in the herbal raw material. In this context, phytochemical profile of Asteracantha longifolia Nees. (syn. Hygrophila spinosa T. Anders.; Hygrophila auriculata [K. Schum.] Heine), an important medicinal herb, was developed using HPTLC technique and was successfully used for evaluating regional and morphological variations. The HPTLC fingerprints were also used for the quantitation of two bioactive markers β-sitosterol and Lupeol in the plant powder. These phytochemical markers were also evaluated from different parts of the plant and from the whole plant collected from different geographical regions. Maximum content of Lupeol was found to be in roots (0.25%), while maximum content of β-sitosterol was found to be in the leaves (0.069%) of Asteracantha longifolia Nees.
Detection and Estimation of alpha-Amyrin, beta-Sitosterol, Lupeol, and n-Triacontane in Two Medicinal Plants by High Performance Thin Layer Chromatography  [PDF]
Saikat S. Mallick,Vidya V. Dighe
Advances in Chemistry , 2014, DOI: 10.1155/2014/143948
Abstract: A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous estimation of four components, namely, alpha-amyrin, beta-sitosterol, lupeol, and n-triacontane from two medicinally important plants, Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. In Ayurveda, both plants have been reported to possess immunomodulatory activity. Chromatographic separation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. was performed on TLC aluminium plates precoated with silica gel using a suitable mobile phase. The densitometric scanning was done after derivatization at λ = 580?nm for α-amyrin, β-sitosterol, and lupeol, and at 366?nm for n-triacontane. The developed HPTLC method has been validated and used for simultaneous quantitation of the four components from the methanolic extracts of whole plant powders of Leptadenia reticulata Wight & Arn. and Pluchea lanceolata (DC.) CB. Clarke. The developed HPTLC method is simple, rapid, and precise and can be used for routine quality control. 1. Introduction Herbal medicines have been used since ages to treat various ailments. Ayurveda is an Indian traditional system of medicine used since ancient times. It has a huge list of herbs used in various forms for treatment of different disease conditions. Owing to the medicinal properties attributed to herbal drugs, it is necessary to maintain their quality and purity, thereby justifying their acceptability in modern system of medicine. Standardisation of these herbal drugs is a challenge to the entire scientific fraternity. However, due to lack of suitable quality control and quality assurance standards for herbal drugs, it becomes difficult to ensure uniformity of their composition which in turn affects the efficacy of their final products. Analytical tools are important for qualitative, semiquantitative, and quantitative phytochemical analysis of herbal drugs and formulations. Chromatographic techniques such as high performance liquid chromatography (HPLC), high performance thin layer chromatography (HPTLC), and gas chromatography (GC) are used to efficiently determine the quality of the herbs by developing fingerprints and estimation of biomarkers. Among the wide choice of chromatographic techniques, HPTLC is a simple, fast, and accurate technique for use, making it advantageous over others for quick assessment of a number of samples simultaneously [1]. In the present research work, HPTLC
Screening of Filamentous Fungi to Identify Biocatalysts for Lupeol Biotransformation  [PDF]
Tatiane C. de Carvalho,Aline M. Polizeli,Izabel C. C. Turatti,Marcela E. Severiano,Carlos E. de Carvalho,Sérgio R. Ambrósio,Ant?nio E. M. Crotti,Uir S. de Figueiredo,Paulo C. Vieira,Niege A. J. C. Furtado
Molecules , 2010, DOI: 10.3390/molecules15096140
Abstract: The goal of the study was to evaluate the ability of filamentous fungi to biotransform the pentacyclic triterpene lupeol. The microbial transformations were carried out in shake flasks in different media. Experiments were also run with control flasks. Samples of each culture were taken every 24 hours, extracted with ethyl acetate, and analyzed by GC-MS. The biotransformation of lupeol by Aspergillus ochraceus and Mucor rouxii afforded two compounds in each culture, which were detected in the cultures developed for more than seven days only in the Koch’s K1 medium. The obtained data demonstrated that A. ochraceus is a good biocatalyst to introduce double bonds in the lupeol structure, whereas M. rouxii exhibits ability to biocatalyze oxygen insertions in that pentacyclic triterpene. Mass spectrometry was demonstrated to be an efficient analytical method to select promising biocatalysts for the compound investigated in this study. The biotransformation processes were influenced by the culture medium and incubation period. The obtained results open the perspective of using A. ochraceus and M. rouxii in pentacyclic triterpene biotransformations.
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