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Synergistic Protection of L-Arginine and Vitamin E On Lipid Peroxidation of Asthenospermic Patients
Sudha Srivastava
International Journal of Fertility & Sterility , 2008,
Abstract: Background: Lipid peroxidation is known to cause various impairments to sperm cells and mayplay a major role in the etiology of male infertility. Asthenospermia is the main factor of maleinfertility and has significantly higher level of peroxidation than in normozoospermic males.Materials and Methods: Using thiobarbituric acid (TBA) assay procedure, we have determinedthe level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa obtainedfrom asthenospermic male semen.Results: An inverse correlation of MDA concentration with sperm motility is observed. Treatmentof cells with L-arginine and vitamin E significantly decreases the MDA concentration and improvesthe sperm motility as compared to that in case of control samples. A combination of L-arginine andvitamin E shows synergistic effect on sperm motility and prevention of lipid peroxidation.Conclusion: L-arginine and vitamin E protect the cells against the loss of sperm motility by lipidperoxidation. Therefore, supplementation of both L-arginine and vitamin E may improve spermmotility and increase the possibility of fertilization in asthenospermic subjects.
The effect of human recombinant leukemia inhibitory factor on sperm motility and survival
Saki Gh,Ghalambor Dezfully F,Sobhani A
Tehran University Medical Journal , 2007,
Abstract: Background: Leukemia inhibitory factor (LIF) is a group of secreted glycoproteins with molecular weights ranging from 38-67 kD, resulting from differential protein glycosylation. LIF is constitutively expressed at high levels in the human fallopian tube epithelium and has an important role in the motility and vitality of sperm. In the present study, the effect of human recombinant LIF on human sperm motility and survival in vitro was investigated.Methods: Normal spermatozoa of 30 fertile men were collected and after preparation were incubated in Ham's F10+FCS 10% medium, containing various concentrations (0, 3, 5, 10, and 50 ng/ml) of LIF at 37 oC under 5% CO2 for 6, 24 and 48 hours. Sperm motion characteristics were measured using a Makler chamber. Sperm survival was determined using the hypoosmotic swelling test. Collected data were analyzed by one-way ANOVA and LSD test using SPSS version 11. The difference in values were considered significant when p<0.05.Results: Sperm motility was significantly higher after 24 h exposure to 5-10 ng/ml LIF (p<0.05). The survival rate of sperm was significantly prolonged when exposed to 50 ng/ml LIF (p<0.05). Nonprogressive motility and survival rate of sperm were significantly higher after 48 h exposure to 50 ng/ml and 10-50 ng/ml LIF, respectively. (p<0.05). There was no significant difference in progressive sperm motility during the 48 h exposure of sperm to the various concentrations of LIF.Conclusion: According to our results, the effect of LIF on sperm motility and survival were dependent on the dose of LIF supplementation and the length of incubation."n
Effect of Nitric Oxide on Ram Sperm Motility In vitro
Hossein Hassanpour,Pejman Mirshokrai,Abolfazl Shirazi,Atefe Aminian
Pakistan Journal of Biological Sciences , 2007,
Abstract: The aim of this study is to investigate the effects of NO on sperm motility of ram. After incubation of normozoospermic samples for 160 minutes in the presence of L-arginine (NOS substrate ), SNP (NO donor), L-NAME (non-selective nitric oxide inhibitor) and packed erythrocytes (NO scavenger), sperm motility assessed in four grades (A, B, C, D). In this study, L-arginine and SNP non-significantly increased progressive motility at low concentration and significantly decreased progressive motility at high concentration. L-NAME and packed erythrocytes dose- dependently decreased progressive sperm motility. It is concluded that sperm motility of ram physiologically depend on nitric oxide action although excess generation of nitric oxide by sperm or exogenous NO could not increase sperm motility and it seems that excess NO provides toxic condition to decrease sperm motility.
Journal of Research in Medical Sciences , 2003,
Abstract: Introduction: CD46 is a membrane cofactor protein (MCP) of complement system wich is present on the membrane of all somatic cells except RBC. It is also present on the inner acrosmal membrane of human sperm. Thus, the aim of this study was to compare the expression of this prote, in on the inner acrosmal membrane of sperms from normospermic and asthenospermic individuals. Method: Semen from 6 normospermic and 17 asthenospermic individuals were examined for CD46 expression. After solublization of sperms in solublizing detergent, the solublized sperm membrane was separated from the rest of cell organelles by centrifugation. Solublized sperm membrane were divide to equal parts and SOS-PAGE gel was canied out in paired on the same gel for each sample. Western blot was carried out on half of the gel and then the nitrocellose papers were stained by a monocolonal Ab and HRP conjugate Ab. The other half were stained by silver stain for identification of MW. Results: After scoring the stained nitrocellose papen in each groups, no statistical significant difference was observed for C046 expression between the two groups. However, a significant Spearmen correlation was observed between CD46 expression and sperm motility (r=0.597, P=0.003). The MW of C046 was between 36 to 45 KD. with a mean of 42 KD. Discussion: This is the first report of a positive Spearmen correlation between sperm CD46 expression and sperm motility which suggest that there might be relation between CD46 expression and sperm motility.
Tale of Fish Sperm and Factors Affecting Sperm Motility: A Review
Advances in Life Sciences , 2011, DOI: 10.5923/j.als.20110101.03
Abstract: Motility is an important function of the male gamete, which allows sperm to actively reach and penetrate the female gamete in organisms with internal and external fertilization. Sexual activity of some fish is generally seasonal and fertilization is external. Sperm, once differentiated in the gonad, remain there completely quiescent until they are released into the external medium, which is either freshwater or sea water. Various parameters such as ion concentrations (K+, Na+, Ca2+), osmotic pressure, pH, and temperature affect motility. In the present paper, we review the roles of these factors on sperm motility in the teleosts. Studying the effects of these factors on teleost sperm can help establish good activation and/or immobilizing media for improving either artificial fertilization or cryopreservation.
In vitro Effect of Oxytocin on the Duration of Sperm Motility and Morphology
H?seyin Baki ?ift
Journal of Animal and Veterinary Advances , 2012,
Abstract: This study was designed to measure the effect of oxytocin on sperm morphology and prolongevity of motility of boar sperm kept at 18? C in diluent. Semen were taken from the same boar and diluted with citrate buffer. Diluted semen was containing 47x10 Sperm mLG . Two mLG aliquots of diluted semen were placed into a series of test tubes. The tubes were added with 20?LG of saline containing oxytocin to give a final concentrationof 5 i.u. oxytocin,mLG were kept as test group while the tubes added just only with 20?LG saline were kept as control group. The tubes were kept at 18?C and at 0, 3rd, 19th, 26th, 42nd, 49th and 56 th h three samples from each group were taken out for motility and morphology measurements. Percentages of zero time motility were taken as 100%. The mean time taken for 15 and 50% decrease in total motility for control and test groups (Mean?SD) were 41.0?1.0 and 35..0?4.8; 25.6?5.0 and 29.0?9.8 respectively. The mean time taken for 6% and 50% decrease in forward motility in control and oxytocin treated group were 33.7?3.0 and 33.4?7.0; 12.4?3.3 and 12.5?3.4 respectively. Neither of the differences in mean times taken for decreases in motilities, were statistically significant. The differences in sperm morphology were not also significant (p>0.05). Sperm motility and morphology are important parameters for fertilizing ability of sperm. These results show that addition of 5 i.u. oyxtocin has no effect on the mean time taken for certain decreases sperm motility or sperm morphology. Therefore oxytocin did not increase fertilizing ability of sperm in-vitro.
Sperm motility and morphology as changing parameters linked to sperm count variations.  [cached]
Dua A,Vaidya S
Journal of Postgraduate Medicine , 1996,
Abstract: Variations in semen analyses of 177 males over a 1 year period were assessed. The average means of total counts, motility, morphology, total motile count and non-motile % were determined for 5 classes of patients ranging from azoospermic to normospermic. Positive relationships between a falling sperm count, a decrease in motility and total motile counts were seen. Also, increasingly, abnormal forms were found with lower sperm counts.
Evidence for the involvement of NGF in human sperm motility  [PDF]
Cui-Ge Shi, Kai Lin, Xiang-Bo Xu, Shu-Cheng Zhang, Ning Wang, Ming Fan
Journal of Biomedical Science and Engineering (JBiSE) , 2012, DOI: 10.4236/jbise.2012.59066
Abstract: Motility is an important physiological characteristic of a mature sperm. Nerve growth factor (NGF) is a key protein for the survival, maintenance and development of the central and peripheral nervous systems. It has been shown that NGF and its receptors TrkA and p75 are widely expressed in the testis, accessory reproductive organ, and the epididymal sperms. These observation have shifted the attention to the role of NGF on male reproductive physiology. In the present study, we found that NGF remarkably increased testicular coefficient in rats subjected to unpredictable chronic mild stress. Furthermore, we investigated the role of NGF on human sperm motility in vitro by CASA. The results showed that the parameters of sperm motility after NGF treatment had significantly increased, the means of VAP, VSL, VCL, BCF and LIN were significantly increased 32% than those of NGF absence, the means of MAD, STR, ALH and WOB had no notable difference. In addition, NGF promotes the sperm motility in a time- and dose-dependent manner. Taken together, our findings suggest that NGF plays a promoted role in sperm motility.
Vildan Karpuz,Asl? G?ktürk,Meral Koyutürk
Marmara Medical Journal , 2007,
Abstract: Objective: The outcomes of ICSI (Intracytoplasmic Sperm Injection) treatments were evaluated and compared with sperm morphology and motility classifications in order to determine whether strict criteria or motility could aid in predicting the ICSI outcomes.Materials and methods: The infertile couples admitted to the clinic, 242 of them were selected and ICSI treatment was performed. In study group, female partners required having at least 5 oocytes at metaphase II and for male partners only the presence of spermatozoa cells in the semen fluid was necessary. Semen analysis and motility was performed according to WHO (World Health Organisation) criteria and sperm morphology was assessed according to Kruger’s criteria.Results: There was no significant difference for the ICSI outcome assessment parameters indicated that fertilization and pregnancy rates between in the groups based on the percentages of sperms morphology and motility.Conclusion: Sperm morphology and motility were accepted as best parameters to evaluate the outcomes of IVF (In Vitro Fertilization). However, our results showed that ICSI outcomes were independent from these valuable parameters for IVF.
Autocrine regulation of human sperm motility by tachykinins
Francisco M Pinto, Cristina G Ravina, Nerea Subiran, Antonio Cejudo-Román, Manuel Fernández-Sánchez, Jon Irazusta, Nicolas Garrido, Luz Candenas
Reproductive Biology and Endocrinology , 2010, DOI: 10.1186/1477-7827-8-104
Abstract: Freshly ejaculated semen was collected from forty-eight normozoospermic human donors. We analyzed the expression of substance P, neurokinin A, neurokinin B, hemokinin-1, NEP and NEP2 in sperm cells by reverse-transcriptase polymerase chain reaction (RT-PCR), western blot and immunocytochemistry assays and evaluated the effects of the neprilysin and neprilysin-2 inhibitor phosphoramidon on sperm motility in the absence and presence of tachykinin receptor-selective antagonists. Sperm motility was measured using WHO procedures or computer-assisted sperm analysis (CASA).The mRNAs of the genes that encode substance P/neurokinin A (TAC1), neurokinin B (TAC3), hemokinin-1 (TAC4), neprilysin (MME) and neprilysin-2 (MMEL1) were expressed in human sperm. Immunocytochemistry studies revealed that tachykinin and neprilysin proteins were present in spermatozoa and show specific and differential distributions. Phosphoramidon increased sperm progressive motility and its effects were reduced in the presence of the tachykinin receptor antagonists SR140333 (NK1 receptor-selective) and SR48968 (NK2 receptor-selective) but unmodified in the presence of SR142801 (NK3 receptor-selective).These data show that tachykinins are present in human spermatozoa and participate in the regulation of sperm motility. Tachykinin activity is regulated, at least in part, by neprilysins.There is now convincing evidence that tachykinins are involved in the regulation of reproductive function [1-8]. Recent data have demonstrated that tachykinin receptors are present in human sperm and are functionally active suggesting a role for the tachykinin system in the regulation of sperm function [9].Mammalian tachykinins comprise a family of regulatory peptides including substance P (SP), neurokinin A (NKA), neurokinin B (NKB) and hemokinin-1 (HK-1) [10-15]. In humans, tachykinins are the products of three different genes. The TAC1 gene gives rise to four different mRNA splicing isoforms (α, β, γ and δ) that encode
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