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High field 13C NMR spectroscopic analysis of the triacylglycerols of Jatropha curcas oil
E.T. Akintayo, E.I. Adeyeye, C.O. Akintayo, P.O. Oyewusi
Bulletin of the Chemical Society of Ethiopia , 2004,
Abstract: Gated decoupled 13C NMR has been employed to determine the acyl composition and acyl positional distribution on the glycerol backbone in the triacylglycerols of Jatropha curcas oil. Results revealed the presence of saturated, oleic and linoleic acids in the oil. Integrals obtained from the allylic carbons were used for semiquantitative analysis of the oil and gave the percentage of total saturated, oleic and linoleic acids in the oil as 27, 41 and 32, respectively. The results obtained by 13C NMR compared favourably with those obtained by gas chromatographic analysis. Analysis of the spectra further revealed that the saturated acyl esters are randomly distributed in the a and b glyceridic positions, with oleic being approximately 60% distrbuted at a glyceridic carbons and linoleic approximately 52% distributed at b glyceridic carbon. KEY WORDS: Gated decoupled 13C NMR, Acyl composition, Acyl positional distribution, Triacylglycerols, Jatropha curcas oil Bull. Chem. Soc. Ethiop. 2004, 18(1), 59-65.
Environmental Impacts of Jatropha curcas Biodiesel in India
Simon Gmünder,Reena Singh,Stephan Pfister,Alok Adheloya,Rainer Zah
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/623070
Abstract: In the context of energy security, rural development and climate change, India actively promotes the cultivation of Jatropha curcas, a biodiesel feedstock which has been identified as suitable for achieving the Indian target of 20% biofuel blending by 2017. In this paper, we present results concerning the range of environmental impacts of different Jatropha curcas cultivation systems. Moreover, nine agronomic trials in Andhra Pradesh are analysed, in which the yield was measured as a function of different inputs such as water, fertilizer, pesticides, and arbuscular mycorrhizal fungi. Further, the environmental impact of the whole Jatropha curcas biodiesel value chain is benchmarked with fossil diesel, following the ISO 14040/44 life cycle assessment procedure. Overall, this study shows that the use of Jatropha curcas biodiesel generally reduces the global warming potential and the nonrenewable energy demand as compared to fossil diesel. On the other hand, the environmental impacts on acidification, ecotoxicity, eutrophication, and water depletion all showed increases. Key for reducing the environmental impact of Jatropha curcas biodiesel is the resource efficiency during crop cultivation (especially mineral fertilizer application) and the optimal site selection of the Jatropha curcas plantations.
High field 13C NMR spectroscopic analysis of the triacylglycerols of Jatropha curcas oil  [cached]
E.T. Akintayo,E.I. Adeyeye,C.O. Akintayo,P.O. Oyewusi
Bulletin of the Chemical Society of Ethiopia , 2004,
Abstract: Gated decoupled 13C NMR has been employed to determine the acyl composition and acyl positional distribution on the glycerol backbone in the triacylglycerols of Jatropha curcas oil. Results revealed the presence of saturated, oleic and linoleic acids in the oil. Integrals obtained from the allylic carbons were used for semiquantitative analysis of the oil and gave the percentage of total saturated, oleic and linoleic acids in the oil as 27, 41 and 32, respectively. The results obtained by 13C NMR compared favourably with those obtained by gas chromatographic analysis. Analysis of the spectra further revealed that the saturated acyl esters are randomly distributed in the α and β glyceridic positions, with oleic being approximately 60% distrbuted at α glyceridic carbons and linoleic approximately 52% distributed at β glyceridic carbon.
Culture in vitro de Jatropha curcas L.  [PDF]
Medza Mvé, SD.,Mergeai, G.,Baudoin, JP.,Toussaint, A.
Biotechnologie, Agronomie, Société et Environnement , 2011,
Abstract: In vitro culture of Jatropha curcas L.. The extension of Jatropha curcas L. cultivation as a biofuel feedstock species requires the distribution of a very large number of plants to the producers in a very short period of time. These plants have to be able to give a high oil yield and be morphologically and phenotypically homogeneous to facilitate cultural operations. If high oil content can be obtained by varietal selection, the achievement of homogeneous material passes by the in vitro propagation. Various methods of mass production of plant material by axenic culture have been published. This study reviews the protocols published for in vitro propagation of J. curcas and discusses their applicability to an industrial scale.
Flower Characteristics and the Yield of Jatropha (Jatropha curcas L.) Accessions  [PDF]
ANDI WIJAYA,SUSANTIDIANA,MUHAMAD UMAR HARUN,HENY HAWALID
HAYATI Journal of Biosciences , 2009,
Abstract: The flower characteristics of a plant is one of the important traits correlated to its productivity. Study on flower characteristics is useful to understand how to increase the crop productivity. The research was conducted at Agro Techno Park Center, Indonesian State Ministry of Research and Technology, Bakung Village, Ogan Ilir Distric South Sumatra from April 2007 to August 2008. The objective of this research was to evaluate the flower characteristics and the yield of jatropha (Jatropha curcas L.) accessions. The research was arranged in a randomized block design, consisted of fifteen accessions of jatropha, with three replicates. The Jatropha accessions were collected from different agro ecosystems located in Sumatra, Java, and Borneo. The results indicated that the evaluated accessions have significant differences in the flower characteristic and the yields. The ratio male–female flower is the most important character for J. curcas L. because it has a significant correlation to the yield.
Effect of phytoprotein treatment on Jatropha curcas for wasteland reclamation
M Debnath, HN Verma
African Journal of Biotechnology , 2008,
Abstract: Jatropha curcas (Euphorbiaceae) is a perennial oil yielding plant. Jatropha is also host to a large number of pathogens such as Cercospora sp. The systemic resistance and overall performance was enhanced by seed soak treatment with leaf extract of Clerodendrum aculeatum. On treatment, the treated plants not only showed systemic resistance but also showed marked enhancement in the plant vegetative growth, rooting, flowering, fruiting and seed formation. Mechanisms underlying this may due the synthesis of a new virus inhibitory phytoprotein produced in cells perceiving signals from systemic resistance inducers or ribosome-inactivating functions of these antiviral proteins. These treated Jatropha curcas can prove to be a miracle plant by turning wasteland into a money making land.
Isolation and Identification of miRNAs in Jatropha curcas
Chun Ming Wang, Peng Liu, Fei Sun, Lei Li, Peng Liu, Jian Ye, Gen Hua Yue
International Journal of Biological Sciences , 2012,
Abstract: MicroRNAs (miRNAs) are small noncoding RNAs that play crucial regulatory roles by targeting mRNAs for silencing. To identify miRNAs in Jatropha curcas L, a bioenergy crop, cDNA clones from two small RNA libraries of leaves and seeds were sequenced and analyzed using bioinformatic tools. Fifty-two putative miRNAs were found from the two libraries, among them six were identical to known miRNAs and 46 were novel. Differential expression patterns of 15 miRNAs in root, stem, leave, fruit and seed were detected using quantitative real-time PCR. Ten miRNAs were highly expressed in fruit or seed, implying that they may be involved in seed development or fatty acids synthesis in seed. Moreover, 28 targets of the isolated miRNAs were predicted from a jatropha cDNA library database. The miRNA target genes were predicted to encode a broad range of proteins. Sixteen targets had clear BLASTX hits to the Uniprot database and were associated with genes belonging to the three major gene ontology categories of biological process, cellular component, and molecular function. Four targets were identified for JcumiR004. By silencing JcumiR004 primary miRNA, expressions of the four target genes were up-regulated and oil composition were modulated significantly, indicating diverse functions of JcumiR004.
PHYTOCHEMICAL STUDY OF CELL CULTURE JATROPHA CURCAS  [cached]
ELFAHMI,ARTRI,KOMAR RUSLAN
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2011,
Abstract: Jatropha curcas belongs to the Euphorbiaceae family which has potential economically. This plant has been reported to contain toxic compounds such as curcin and phorbol ester and its derivatives. These compounds may become a problem if J. curcas will be explored as a source of biofuel. In order to provide safety plants, the research on the study of phytochemical and initiation of cell and organ culture have been carried out. J curcas which has been collected from different regions in Indonesia showed to contain relatively the same profile of chemical contents. Dominant compounds that were detected by GCMS are hidrocarbon such as 2-heptenal, decadienal, hexsadecane, pentadecane, cyclooctane etc, fatty acid such as oktadecanoate acid, etthyl linoleate, ethyl stearate, heksadecanoate acid and steroid such as stigmasterol, fucosterol, sitosterol. No phorbol ester and its derivatives have been detected yet by the GCMS method. Callus and suspension cultures of J. curcas have been established to be used for further investigation.
Jatropha curcas L.: Visions and Realities  [PDF]
Martin Grass
Journal of Agriculture and Rural Development in the Tropics and Subtropics , 2009,
Abstract: Since several years Jatropha is experiencing a renaissance. The main drivers for this development are the biofuel boom in general and the special attributes of Jatropha itself. This paper discusses the current knowledge as well as expectations of Jatropha and the consequential outcomes starting with data availability and quality followed by economic and political needs and constraints.
Anti termite activity of Jatropha curcas Linn. biochemicals
N Singh, A Sushilkumar
Journal of Applied Sciences and Environmental Management , 2008,
Abstract: Jatropha curcas oil and its toxic fraction were evaluated at different dilutions i.e., 1%,5%,10%,20% against Microcerotermes beesoni, the test termite. The maximum wood protection against termites of both the treatments were obtained at their highest concentration i.e. 20%. The weight loss ranged from 18.77% to 48.80% at concentrations of 20% to 1% of Jatropha curcas oil formulation. The protection afforded by toxic fraction was enhanced, with the % weight loss ranging from 10.48 to 35.19. However, all the treatments proved to be effective over the control (50.84%)
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