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Correlation between rapid urease test and pathohistological gastrobiopsy finding with positive immunological test in detecting Helicobacter pylori infection  [PDF]
Naumov Ivana,Fenjve?i Atila
Medicinski Pregled , 2011, DOI: 10.2298/mpns1108413n
Abstract: Introduction. Helicobacter pylori is a bacterium that has been in the limelight of gastroenterologists and oncologists worldwide since its immediate effect on the development of gastritis, ulcer disease, mucosa-associated lymphoid tissue lymphoma and gastric cancer was proved. The aim of this study was to determine the correlation between the positive immunological test, rapid urease test and pathohistological finding in detecting Helicobacter pylori infection of the gastric mucosa. Material and Methods. This retrospective study included 250 endoscopic findings of subjects having undergone endoscopy in the first half of 2010 at the Ward of Gastroenterology, Department of Internal Diseases, Hospital in Senta. The endoscopy procedure was performed by Fujinon endoscopes and the fast urease test was done by the Cambridge Life Science set from England. The immunological test was carried out by the Vidas apparatus applying the Enzyme-Linked Fluorescent Assay (ELFA) technique and the staining for the pathohistological analysis was done by the modified Giemsa method. Results. The paper presents the results obtained by endoscopic examination of a group of 250 patients performed at the Ward of Gastroenterology, Department of Internal Diseases, Hospital in Senta. The endoscopic findings were the first to be analyzed then the rapid urease test findings, which showed that 95 (38%) patients were positive and 155 (62%) patients were negative; whereas the immunologic test for Helicobacter pylori was positive in all 250 patients (100%). The histological test for Helicobacter-Like Organism was positive in 105 subjects (42%), whereas it was negative in 145 patients, that being 58% of all the cases. Sensitivity, specificity and predictability of the serological test and the rapid urease test were calculated according to the pathohistological finding as the ”gold standard” and they were found to be: sensitivity 100% and specificity 0% for the serological test and sensitivity 90% and specificity 100% for the rapid urease test. The immunologic test was not correlated with other findings, and there was a high level of correlation between the rapid urease test and histological test (r=0.927589261). The t-test was calculated to be 36.16513; p=0.0001; that indicating that the correlation coefficient was statistically significant (p>0.01). Discussion. The obtained results were compared with the data found in the available literature sources. Conclusion. It can be concluded that the most competent technique for the optimal diagnosis of Helicobacter pylori infection is the invasive
HISTORY OF HELICOBACTER PYLORI INFECTION  [cached]
Tomica Milosavljevi?,Aleksandar Nagorni
Acta Facultatis Medicae Naissensis , 2003,
Abstract: Helicobacter pylori was identified in 1979, but cultivated in Australia in 1982. Warren and Marshall were responsible for discovery. But, in the previous one hundred years, a lot of scientists tried to prove hipothesis about possible association between peptic ulcer disease and spiral microorganism. Numerous authors were objectivated H. pylori on some way. Consensus Conferences in Maastricht in 1996 and 2000 and First Consensus of Yugoslav Gastroenterologic Association gave us recommendation and guideline for diagnostics and treatment of H. pylori infection.
The relationship between helicobacter pylori infection and gastro-esophageal reflux disease
Batool M. Mahdi
North American Journal of Medical Sciences , 2011,
Abstract: Background: Gastro-esophageal reflux disease is a common condition, affecting 25%-40% of the population. Increasing attention has been paid to the relationship between Helicobacter pylori infection and reflux esophagitis. Aim: The aim of this study was to investigate the association between CagA+ H. pylori and endoscopically proven gastro-esophageal reflux disease. Patients and Methods: The study group included 60 hospital patients with gastro-esophageal reflux disease between 2007 and 2009 as compared with 30 healthy patients from a control group that was age and sex matched. Helicobacter pylori CagA+ was identified by an immunological test (Immunochromatography test) (ACON, USA). Results: Helicobacter pylori CagA+ was present in 42/60 (70%) of the patients with gastro-esophageal reflux disease and in 11/30 (36.6%) patients in the control group (p=0.002). The Odds ratio = 0.8004 with 95% Confidence Interval = from 0.3188 to 2.0094. The relative risk=1.35 that indicates an association between Helicobacter pylori and disease. Conclusions: The presence of Helicobacter pylori is significantly increased in patients with gastro-esophageal reflux disease as compared with the control group.
Helicobacter pylori cag-Pathogenicity Island-Dependent Early Immunological Response Triggers Later Precancerous Gastric Changes in Mongolian Gerbils  [PDF]
Tobias Wiedemann, Eva Loell, Susanna Mueller, Mechthild Stoeckelhuber, Manfred Stolte, Rainer Haas, Gabriele Rieder
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0004754
Abstract: Infection with Helicobacter pylori, carrying a functional cag type IV secretion system (cag-T4SS) to inject the Cytotoxin associated antigen (CagA) into gastric cells, is associated with an increased risk for severe gastric diseases in humans. Here we studied the pathomechanism of H. pylori and the role of the cag-pathogenicity island (cag-PAI) for the induction of gastric ulcer and precancerous conditions over time (2–64 weeks) using the Mongolian gerbil model. Animals were challenged with H. pylori B128 (WT), or an isogenic B128ΔcagY mutant-strain that produces CagA, but is unable to translocate it into gastric cells. H. pylori colonization density was quantified in antrum and corpus mucosa separately. Paraffin sections were graded for inflammation and histological changes verified by immunohistochemistry. Physiological and inflammatory markers were quantitated by RIA and RT-PCR, respectively. An early cag-T4SS-dependent inflammation of the corpus mucosa (4–8 weeks) occurred only in WT-infected animals, resulting in a severe active and chronic gastritis with a significant increase of proinflammatory cytokines, mucous gland metaplasia, and atrophy of the parietal cells. At late time points only WT-infected animals developed hypochlorhydria and hypergastrinemia in parallel to gastric ulcers, gastritis cystica profunda, and focal dysplasia. The early cag-PAI-dependent immunological response triggers later physiological and histopathological alterations towards gastric malignancies.
Clinical Significance of Peripheral Blood T Lymphocyte Subsets in Helicobacter pylori-Infected Patients
Yuka Satoh,Hatsue Ogawara,Osamu Kawamura,Motoyasu Kusano,Hirokazu Murakami
Gastroenterology Research and Practice , 2012, DOI: 10.1155/2012/819842
Abstract: Background. Helicobacter pylori chronically colonizes gastric/duodenal mucosa and induces gastroduodenal disease and vigorous humoral and cellular immune responses. Methods. In order to clarify the immunological changes induced by this infection, we determined the percentage and, as indicated, ratios of the following cells in peripheral blood of 45 H. pylori-infected patients and 21 control subjects: CD4
Colonization by Helicobacter pylori of leprosy patients in Spain: immunomodulation to low molecular weight antigens of H. pylori
Fernando, Neluka;Torres, Pedro;Vaira, Dino;Holton, John;
Memórias do Instituto Oswaldo Cruz , 2010, DOI: 10.1590/S0074-02762010000500014
Abstract: we studied the prevalence of helicobacter pylori in patients with leprosy and the effects of co-infection on the immune response to helicobacter antigens in the polar groups of leprosy (lepromatous and tuberculoid). we showed that there is no difference in the prevalence of h. pylori in patients with leprosy as compared to a non-leprosy population. we also demonstrated that the immune response to low molecular weight h. pylori antigens (35, 26 and 19 kda) differs in patients with lepromatous as compared to those with tuberculoid leprosy. in lepromatous leprosy, we show that there is a higher prevalence of the 35 and 26 kda antigens, but a lower prevalence of the 19 kda antigen. these immunological results are consistent with previous histopathological studies illustrating a more severe gastrointestinal inflammation in lepromatous patients; importantly, a response to the 35 kda antigen is recognized as a marker for the development of ulcerative disease.
Validation of a rapid stool antigen test for diagnosis of Helicobacter pylori infection
Silva, Joyce Matie Kinoshita da;Villares, Cibele Aparecida;Monteiro, Maria do Socorro;Colaúto, Carlos;Santos, Anibal Ferreira dos;Mattar, Rejane;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2010, DOI: 10.1590/S0036-46652010000300002
Abstract: the aim of this study was to validate the rapid lateral flow helicobacter pylori stool antigen test (one step h. pylori antigen test, acon laboratories, san diego, usa; prime diagnostics, s?o paulo), using 13c-urea breath test as the gold standard for h. pylori infection diagnosis. a total of 98 consecutive patients, asymptomatic or dyspeptic, entered the study. sixty-nine were women, with a mean age of 45.76 ± 14.59 years (14 to 79 years). in the h. pylori-positive group, the rapid stool antigen test detected h. pylori antigen in 44 of the 50 positive patients (sensitivity 88%; 95% ci: 75.7-95.5%), and six false-negative; and in the h. pylori-negative group 42 presented negative results (specificity 87.5%; 95% ci: 74.7-95.3%), and six false-positive, showing a substantial agreement (kappa index = 0.75; p < 0.0001; 95% ci: 0.6-0.9). forty four of fifty patients that had positive stool antigen were h. pylori-positive, the ppv of the stool antigen test was 88% (95% ci: 75.7-95.5%), and 42 patients with negative stool antigen test were h. pylori-negative, the npv of the stool antigen test was 87.5% (95% ci: 74.7-95.3%). we conclude that the lateral flow stool antigen test can be used as an alternative to breath test for h. pylori infection diagnosis especially in developing countries.
Skuteczno immunoenzymatycznego testu Premier Platinum HpSA w wykrywaniu zaka eń wywo ywanych przez Helicobacter pylori
Leokadia B?k-Romaniszyn,Monika Wi?niewska,Karina Malczewska,El?bieta Czkwianianc
Pediatria Wspó?czesna , 2000,
Abstract: Antygeny pa eczek Helicobacter pylori wywo uj cych choroby o dka i dwunastnicy ulegaj koncentracji wjelicie grubym. S dzi si , i ich wykrycie w kale potwierdza aktualne zaka enie H. pylori. Brak takich antygenów w kale osób poddanych leczeniu mo e by wska nikiem eradykacji bakterii. Oceniano przydatno immunoenzymatycznego testu Premier Platinum HpSA (Meridian Diagnostics) w wykrywaniu antygenów H. pylori w kale w monitorowaniu zaka eń wywo ywanych przez te drobnoustroje. Znaczna zgodno wyników testu Premier Platinum HpSA z rezultatami badań inwazyjnych potwierdza jego przydatno w se-rodiagnostyce zaka eń H. pylori, chocia niedostateczna liczba pa eczek H. pylori w nab onku o dka mo e by przyczyn wyników fa szywie ujemnych.
Immunoblotting for the serodiagnosis of Helicobacter pylori ?infection in Brazilian patients with and without gastric carcinoma
Rocha, Andreia MC;Rocha, Gifone A;Leite, Janise L;Lisboa, Renato LL;Silva, Patrícia VO;Queiroz, Dulciene M M;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000200013
Abstract: we evaluated the performance of a commercial immunoblotting in the serodiagnosis of helicobacter pylori infection in brazilian patients. the presence of anti-h. pylori antibodies was also investigated in a group of 20 duodenal ulcer patients after successful treatment. one hundred and ninety one patients were studied. among the 164 infected patients, 46 had gastric carcinoma. the duodenal ulcer patients were treated with antimicrobial drugs and the eradication of the microorganism was confirmed in all of them one month after the end of the treatment by the 13c-urea breath test. sera were assayed for h. pylori antibodies using the helicoblot 2.0 (genelabs diagnostics, singapore). the sensitivity, specificity, positive, and negative predictive values of the test were 93.9%, 92.6%, 98.7%, and 71.4%, respectively. the sensitivity of the test was similar in patients with (93.5%) and without (95.7%) gastric carcinoma. twenty-four months after the end of the treatment, the band of 116 kda was still detected in one of the patients. in conclusion, the helicoblot 2.0 is an accurate test to diagnose h. pylori infection and although it can not be employed to monitor the bacterium eradication, it may be useful for diagnosing past infection, especially in gastric carcinoma patients.
Helicobacter pylori: recent advances in the study of its pathogenicity and prevention
Aguilar,Germán R.; Ayala,Guadalupe; Fierros-Zárate,Geny;
Salud Pública de México , 2001, DOI: 10.1590/S0036-36342001000300010
Abstract: helicobacter pylori has acquired great importance during the last two decades, after being recognized as an important pathogen that infects a great portion of the human population. this microorganism is recognized as the main causal agent of chronic gastritis and duodenal ulcers, and it is associated with the subsequent development of gastric carcinoma. the pathogenic mechanisms of h. pylori and their relation to gastric ailments have not been clearly defined. however, at present it is well established that urease, vacuolating cytotoxin vaca, and the pathogenicity island (cag pai) gene products, are the main factors of virulence of this organism. thus, individuals infected with strains that express these virulence factors probably develop a severe local inflammation that may induce the development of peptic ulcer and gastric cancer. the way the infection spreads throughout the world suggests the possibility that there are multiple pathways of transmission. due to the importance that h. pylori has acquired as a human pathogen, laboratories worldwide are attempting to develop a vaccine that confers long-term immunological protection against infection by this microorganism. hence, the objective of this review is to present the most relevant findings of the biology of h. pylori and its interaction with the human host. the full version of this paper is available too at: http://www.insp.mx/salud/index.html
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