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Cervical acid phosphatase detection: A guide to abnormal cells in cytology smear screening for cervical cancer  [cached]
Deb Prabal,Iyer Venkateswaran,Bhatla Neerja,Markovic O
Journal of Cytology , 2008,
Abstract: Background: Cervical acid phosphatase-Papanicolaou (CAP-PAP) test has recently been described for detection of acid phosphatase enzyme in abnormal squamous cells, and has been proposed as a biomarker-based technology for the screening of cervical cancer. Materials and Methods: Eighty-one consecutive cervical smears were subjected to routine Papanicolaou (Pap) staining as well as CAP-PAP, which combined cytochemical staining for acid phosphatase with modified Pap stain. Statistical evaluation of its utility was examined. Results: Of 81 smears, 16 (19.75%) showed the presence of mature squamous cells with acid phosphatase by CAP-PAP technique and were considered positive. Of these 16, atypical squamous cells of undetermined significance (ASCUS) or above were initially diagnosed in five of the corresponding routine Pap smears. After re-evaluation with CAP-PAP, eight of the routine Pap smears were considered to have ASCUS or above. Of these eight, three were reported as low-grade squamous intraepithelial lesions and five as ASCUS on conventional Pap smears. The remaining 8/16 CAP-PAP-positive cases were negative for atypical squamous cells on the corresponding Pap smears. None of the CAP-PAP-negative smears were positive on routine Pap smear screening. Conclusions: This study highlights the efficacy of CAP-PAP in quality assurance of cervical smear screening. It is also an inexpensive method for segregating smears for subsequent re-screening. In the absence of trained cytologists in peripheral laboratories, this technique can be adopted for identifying smears that would require proper evaluation.
Tartrate-resistant acid phosphatase as a biomarker of bone turnover in dog
Sousa, C.P;Nery, F;Azevedo, J.T;Viegas, C.A;Gomes, M.E;Dias, I.R;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2011, DOI: 10.1590/S0102-09352011000100007
Abstract: values of serum tartrate-resistant acid phosphatase ( trap) activity were obtained in adult dogs and its biological variability was assessed. nine healthy skeletally mature portuguese podengo dogs were used for the determination of trap, total and bone alkaline phosphatase serum activities, and also to study their relationship with serum minerals, namely calcium (ca), phosphorous (p), and magnesium (mg). the serum trap activity was 2.19±0.56iu/ml, with intra-individual variation of 18.3% and inter-individual variation of 25.6%. significant correlations were observed between serum trap activity and ca (r=-0.3431; p<0.05), ca and mg (r=-0.787; p<0.01), and trap and mg (r=0.397; p<0.05). the results indicate that serum trap activity in dog could be of great value in research and in clinical practice, providing complementary non-invasive information on bone metabolism
Use of acid phosphatase as biomarker during the castor bean seeds germination (ricinus communis)  [PDF]
Paulo Afonso Granjeiro,Carmen Ferreira Veríssima,Hiroshi Aoyama
Engenharia Ambiental : Pesquisa e Tecnologia , 2008,
Abstract: One of the main oil crop of prominent social and economic importance is to mamoneira (Ricinus communis L.); with countless application in the industry and agricultural. Broadly it distributed in Brazil; his cultivation can be an alternative of sustainability in the Brazilian northeast. It know the physiological and biochemical mechanisms of the germination they are important for the best utilization of the plant. The objective of this work was use acid phosphatase as biomarker during the germination. In the rough extract occurred the dosage of the activity for pNPP; Tyr-Pi and PPi; determination of protein and inorganic phosphatse. The peak of activity for pNPP was in the seventh day; for PPi and Tyr-Pi in the ninth and for PEP in the fifth. The concentration of protein increased according to the days of germination; with peak of activity in the eighth day; being coincidental with the peaks of the activities for the substrates. The content of inorganic phosphate diminished with the time of germination and after the third day occurred a fall accentuated of its concentration. We concluded that acid phosphatase is important for the germination of the seeds and his paper is related with the mobilization of inorganic phosphate; the main nutrients for the development.
Biomarkers of Cervical Dysplasia and Carcinoma
Sonya J. Hwang,Kenneth R. Shroyer
Journal of Oncology , 2012, DOI: 10.1155/2012/507286
Abstract: Although cervical cytology screening has decreased the incidence of cervical cancer in industrialized countries, HPV-related cervical disease, including premalignant and malignant lesions, continues to represent a major burden on the health care system. Some of the problems include the potential for either under- or overtreatment of women due to decreased specificity of screening tests as well as significant interobserver variability in the diagnosis of cervical dysplastic lesions. Although not completely elucidated, the HPV-driven molecular mechanisms underlying the development of cervical lesions have provided a number of potential biomarkers for both diagnostic and prognostic use in the clinical management of these women.
Virus-related inflammatory bowel disease oncogenesis: HPV-related cervical dysplasia and EBV-related intestinal lymphoma  [cached]
K.H. Katsanos,E.V. Tsianos
Annals of Gastroenterology , 2007,
Abstract: Key words: Inflammatory bowel disease, cervical intraepithelial neoplasia, cervical dysplasia, human papilloma virus, Epstein Barr virus
Role of the Vaginal Microbiological Ecosystem and Cytokine Profile in the Promotion of Cervical Dysplasia: A Case–Control Study  [PDF]
Kian Behbakht,Jennifer Friedman,Ira Heimler,Alla Aroutcheva,Jose Simoes,Sebastian Faro
Infectious Diseases in Obstetrics and Gynecology , 2002, DOI: 10.1155/s1064744902000200
Abstract: Objective: To identify alterations in the cytokine profile and microbial ecosystem of the vagina in association with cervical dysplasia.
Molecular tests to detect human papillomavirus infection in patients with cervical dysplasia and invasive cervical cancer in Saudi Arabia
Sait KH,Gazzaz FS
Pathology and Laboratory Medicine International , 2011,
Abstract: Khalid H Sait1, Faten S Gazzaz21Obstetrics and Gynecology Department, 2Medical Virology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaPurpose: The aim of this study was to determine the actual human papillomavirus (HPV) subtype that presents in cervical dysplasia and invasive carcinoma in the Saudi population, and the feasibility of using Hybrid Capture 2 technique (HC2) on biopsy specimens to detect certain HPV subtypes.Patients and methods: A prospective study was conducted from March 2007 to December 2008. The subjects studied were women with a mean age of 48.18 years, who attended the hospital for cervical biopsy due to the suspected diagnosis of cervical dysplasia or an invasive disease, based on previous suspicious Pap smear. HPV DNA hybridization by HC2 was performed on the cervical biopsies of these patients, to detect HPV infection.Results: During the period of this study, 45 patients had cervical biopsies taken for HPV testing. Seven patients had a negative HC2 result and were found to have no cervical dysplasia on the final pathology review. Seventeen cases with cervical dysplasia and 21 patients with invasive disease were presented; the mean age was 48 years. HC2 testing for HPV were found to be positive in patients with cervical dysplasia, invasive carcinoma, and all in 5 (29.4%), 13 (61.9%) and 18 (47.4%), respectively. The sensitivity of the test is 47% and specificity is 100%.Conclusion: The use of molecular detection of HPV DNA by HC2 in biopsy is feasible and effective. These results confirm the finding that HPV contributes to the etiology of cervical cancer in Muslim society.Keywords: HPV, subtyping, cervical neoplasia
Molecular tests to detect human papillomavirus infection in patients with cervical dysplasia and invasive cervical cancer in Saudi Arabia
Sait KH, Gazzaz FS
Pathology and Laboratory Medicine International , 2011, DOI: http://dx.doi.org/10.2147/PLMI.S23059
Abstract: lecular tests to detect human papillomavirus infection in patients with cervical dysplasia and invasive cervical cancer in Saudi Arabia Original Research (3004) Total Article Views Authors: Sait KH, Gazzaz FS Published Date July 2011 Volume 2011:3 Pages 25 - 29 DOI: http://dx.doi.org/10.2147/PLMI.S23059 Khalid H Sait1, Faten S Gazzaz2 1Obstetrics and Gynecology Department, 2Medical Virology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia Purpose: The aim of this study was to determine the actual human papillomavirus (HPV) subtype that presents in cervical dysplasia and invasive carcinoma in the Saudi population, and the feasibility of using Hybrid Capture 2 technique (HC2) on biopsy specimens to detect certain HPV subtypes. Patients and methods: A prospective study was conducted from March 2007 to December 2008. The subjects studied were women with a mean age of 48.18 years, who attended the hospital for cervical biopsy due to the suspected diagnosis of cervical dysplasia or an invasive disease, based on previous suspicious Pap smear. HPV DNA hybridization by HC2 was performed on the cervical biopsies of these patients, to detect HPV infection. Results: During the period of this study, 45 patients had cervical biopsies taken for HPV testing. Seven patients had a negative HC2 result and were found to have no cervical dysplasia on the final pathology review. Seventeen cases with cervical dysplasia and 21 patients with invasive disease were presented; the mean age was 48 years. HC2 testing for HPV were found to be positive in patients with cervical dysplasia, invasive carcinoma, and all in 5 (29.4%), 13 (61.9%) and 18 (47.4%), respectively. The sensitivity of the test is 47% and specificity is 100%. Conclusion: The use of molecular detection of HPV DNA by HC2 in biopsy is feasible and effective. These results confirm the finding that HPV contributes to the etiology of cervical cancer in Muslim society.
The tillage effect on the soil acid and alkaline phosphatase activity  [cached]
Lacramioara Oprica,Zenovia Olteanu,Simona Isabela Dunca,Maria-Magdalena Zamfirache
Analele ?tiin?ifice Ale Universit??ii Alexandru Ioan Cuza din Ia?i,Sectiunea II A : Genetica si Biologie Moleculara , 2011,
Abstract: Phosphatases (acid and alkaline) are important in soils because these extracellular enzymes catalyze the hydrolysis of organic phosphate esters to orthophosphate; thus they form an important link between biologically unavailable and mineral phosphorous. Phosphatase activity is sensitive to environmental perturbations such as organic amendments, tillage, waterlogging, compaction, fertilizer additions and thus it is often used as an environmental indicator of soil quality in riparian ecosystems. The aim of the study was to assess the effect of tillage systems on phosphatases activity in a field experiment carried out in Ez reni farm. The phosphatase activitiy were determined at two depths (7-10 cm and 15-25cm layers) of a chernozem soil submitted to conventional tillage (CT) in a fertilised and unfertilised experiment. Monitoring soil alkaline phosphatase activity showed, generally, the same in fertilized soil profiles collected from both depths; the values being extremely close. In unfertilized soils, alkaline phosphatase activity is different only in soils that were exposed to unconventional work using disc harrows and 30cm tillage. Both works type (no tillage and conventional tillage) cause an intense alkaline phosphatase activity in 7-10 cm soil profile. Acid phosphatase activity is highly fluctuating in both fertilized as well unfertilized soil, this enzyme being influenced by the performed works.
Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions
Dolores López-Morales, Julio Reyes-Leyva, Gerardo Santos-López, Edgar Zenteno, Verónica Vallejo-Ruiz
Diagnostic Pathology , 2010, DOI: 10.1186/1746-1596-5-74
Abstract: Lectin histochemistry was used to examine the expression and distribution of sialic acid in different grades of cervical neoplasia. We applied Maackia amurensis lectin, which interacts with α2,3-linked sialic acid and Sambucus nigra lectin specific for α2,6-linked sialic acid.The histochemical analysis showed that α2,3-linked sialic acid and α2,6- linked sialic acid increased in intensity and distribution in concordance with the grade of squamous intraepithelial lesion (SIL). These results are in concordance with a previous study that reports increased RNAm levels of three sialyltransferases.These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. These findings provide the basis for more detailed studies of the possible role of cell surface glycoconjugates bearing sialic acid in the cellular cervix transformation.It is well known that tumorigenesis and metastasis are frequently associated with altered structure and expression of oligosaccharides on cell surface glycoproteins and glycolipids [1-6]. Sialylated glycoconjugates expression has been shown to change during development, differentiation, and disease and oncogenic transformation. A general increase in sialylation of cell surface glycoconjugates of carcinoma cells has been detected [7]. These changes in sialylation are related to invasion and metastasis [8-11].Different studies in serum from patients with cervical cancer showed that sialic acid is elevated even in the early stages of carcinoma, and levels of these glycans decreased to near normal levels after radiotherapy [12,13]. Total sialic acid concentration of cervical tissue has been analyzed. Results showed a slight elevation in benign inflammatory lesions, moderate elevation in severe dysplasia and preinvasive carcinoma and high elevation in invasive cervical carcinoma [14]. Sialic acids are widely distributed in nature as terminal sugars of oligosaccharides attached
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