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SOAKING AND GERMINATION PERCENTAGE OF CRAMBE SEEDS (Crambe abyssinica) EMBEBI O E GERMINA O DE SEMENTES DE CRAMBE (Crambe abyssinica)
Renato Adriane Alves Ruas,Geovane Barbosa do Nascimento,Erwing Paiva Bergamo,Rouves Humberto Daur Junior
Pesquisa Agropecuária Tropical , 2009, DOI: 10.5216/pat.v40i1.6893
Abstract: The pericarp present in some seeds can cause high lack of germination uniformity or even no germination. Thereby, the objective of this study was to evaluate the imbibition and germination percentage of C. abyssinica seeds. The experiment took place in the Federal Institute of Education, Science, and Technology of Goiás State, Morrinhos Campus, Brazil (17°48’27,59”S, 49°12’23,87”W, altitude 770 m). Imbibition and germination percentage of C. abyssinica, MS Brilhante cultivar, with and without pericarp, were evaluated. To verify the imbibition percentage, a completely randomized experimental design in factorial scheme of 5x2 was used, with five seed weighing periods (2, 4, 6, 8, and 10 hours of soaking) and two types of seeds (with or without pericarp), with four replications. To determine germination percentage, two lots of seeds, with and without pericarp, with 50 seeds each, were selected. Seeds were packed in paper towel sheets and received an average of 0,02 L of water. It was concluded that, although the pericarp of C. abyssinica seeds is permeable to water, its withdrawal doubles the germination percentage, if compared to seeds with pericarp. KEY-WORDS: Biofuel; vegetal oil; pericarp. O pericarpo existente em algumas sementes pode proporcionar elevada falta de uniformidade de germina o, ou mesmo ausência de germina o. Assim, objetivou-se, com este trabalho, avaliar o percentual de embebi o e germina o de sementes de C. abyssinica. O experimento foi realizado no Instituto Federal de Educa o, Ciência e Tecnologia Goiano, Campus Morrinhos, GO (17°48’27,59”S, 49°12’23,87”W e altitude de 770 m). Foram realizadas avalia es sobre o percentual de embebi o e germina o de sementes de
Accelerated aging test to evaluate the quality of crambe (Crambe abyssinica Hochst - Brassicaceae) seed physiology Ensayo envejecimiento acelerado para evaluar la calidad fisiológica de las semillas de crambe (Crambe abyssinica Hochst - Brassicaceae)
Elias Terra Werner,José Carlos Lopes,Diego Gomes Junior,Jaquelini Luber
IDESIA , 2013,
Abstract: The present study aimed to evaluate the efficiency of the accelerated aging test to determination of the physiological potential of the Crambe abyssinica Hochst seeds, from two harvests (2009 and 2010). Were evaluated: water content, germination and vigor by germination speed index, accelerated aging in temperatures of 41, 43 and 45 °C for 24, 48, 72 and 96 hours, fresh and dry mass. The experiment was conducted in a 2x3x5 factorial (batches x temperature x exposure time), in a completely randomized design, with four replicates of 25 seeds. The fresh and dry mass were not effective for distinguish the physiological quality of Crambe abyssinica seeds. The accelerated aging test influences, similarly, the percentage of germination and vigor, with greater sensitivity when the seeds treated with temperatures of 41 or 43 °C, for 72 h, and evidenced promising role to a stratification of the physiological quality of crambe seeds batches with similar germination. Este estudio tuvo como objetivo evaluar la eficacia de la prueba de envejecimiento acelerado, para determinar los efectos fisiológicos de semillas Crambe abyssinica Hochst, un montón de dos cosechas (2009 y 2010). Se evaluaron: contenido de agua, la germinación y el vigor por el índice de velocidad de la germinación, envejecimiento acelerado a 41, 43 y 45 °C durante períodos de 24, 48, 72 y 96 horas, el peso fresco y el peso seco. El experimento se llevó a cabo en el esquema factorial 2x3x5 (mucha x temperatura x tiempo de exposición), en un dise o completamente al azar, con cuatro repeticiones de 25 semillas. El peso fresco y peso seco no son eficientes para diferenciar la calidad fisiológica de las semillas de Crambe abyssinica. Las influencias de envejecimiento acelerado, de manera similar, el porcentaje de germinación y el vigor, con una mayor sensibilidad cuando las semillas son tratadas con temperaturas de 41 o 43 °C durante 72 h, lo que indica un papel prometedor para la estratificación de la calidad fisiológica de la semilla crambe de lotes con germinación similar.
Influence of pre-germination treatments and temperature on the germination of crambe seeds (Crambe abyssinica Hochst) Influência de tratamentos pré-germinativos e de temperaturas na germina o de sementes de crambe (Crambe abyssinica Hochst)
Lima Deleon Martins,Felipe Pianna Costa,José Carlos Lopes,Wagner Nunes Rodrigues
IDESIA , 2012,
Abstract: The objective of this study was to evaluate the influence of pre-germination treatments and temperature on the germination of crambe seed (Crambe abyssinica Hochst). The completely randomized design was used in a 3 x 3 x 2 factorial (physical treatments x chemical treatments x temperature). The chemical treatments consisted of paper soaked in gibberellic acid (GA3), potassium nitrate (KNO3) and water. The physical treatments consisted of maintaining the seed intact, mechanical scarification and seed coat removal. Temperatures of 25 and 30 °C were used in the completely randomized design. The variables studied were germination speed index, germination percentage, seed length and seedling dry mass. In the studied conditions, the percentage and rate of germination, length and seedling dry weight of seeds of C. abyssinica Hochst were enhanced by removal of the integument, in soaking with GA3 at 25 °C. Objetivou-se com o presente trabalho, avaliar a influência de tratamentos pré-germinativos e de temperaturas na germina o de sementes de crambe (Crambe abyssinica Hochst), em esquema fatorial 3 x 3 x 2 (tratamentos químicos x tratamentos físicos x temperatura). Sendo os tratamentos químicos: ácido giberélico (GA3), nitrato de potássio (KNO3) e água. E os tratamentos físicos: semente intacta, escarifica o mecanica e a retirada do tegumento. Foram utilizadas as temperaturas de 25 e 30 °C em delineamento inteiramente casualizado. Estudou-se as seguintes variáveis: índice de velocidade e porcentagem de germina o, comprimento e massa seca das plántulas. Nas condi es de estudo, a porcentagem e o índice de velocidade de germina o, o comprimento e a massa seca de plántulas das sementes de C. abyssinica Hochstforam incrementados com a remo o do tegumento, sob o umedecimento com GA3 na temperatura de 25 °C.
FREQUêNCIA DE GERMINA O DE SEMENTES DE CRAMBE (Crambe abyssinica Hochst.) SOB INFLUêNCIA DE TRATAMENTOS PRé-GERMINATIVOS E DE TEMPERATURAS
COSTA, Felipe Pianna,MARTINS, Lima Deleon Martins,LOPES, José Carlos
Nucleus , 2010,
Abstract: Biodiesel appears as a sustainable alternative to oil and its derivatives, because that productionis obtained from renewable sources such as oilseeds. The specie Crambe abyssinica has a structure calledpericarp. In some cases, the pericarp can cause high nonuniform germination in certain species or nogermination. Thus, the aim of this work is compare frequencies of germination in seeds of crambe on theinfluence of pre-germination treatments and temperatures. The statistical design used in the experiment was acompletely randomized design in factorial (3) chemical x (3) physical x (2) temperature treatments, fourrepetitions with 25 seeds per experimental unit. The treatments is chemical: (GA3), (KNO3) and (water);physical: intacts, scarified and dehulled; (2) temperature: 25oC and 30oC. Results showed that the dehulled seedsof crambe increase frequencies of germination in all of temperatures and chemical treatments.O biodiesel figura como uma alternativa sustentável ao petróleo e seus derivados, uma vez que suaprodu o é obtida de fontes renováveis como plantas oleaginosas. A espécie Crambe abyssinica possui umaestrutura tegumentar denominada pericarpo, o que pode, em alguns casos, ocasionar elevada desuniformidade ouaté mesmo ausência de germina o. Sendo assim, objetivou-se com o presente trabalho comparar freqüências degermina o em sementes de crambe sob influência de tratamentos pré-germinativos e de temperaturas. Odelineamento experimental utilizado foi o inteiramente casualisado, em esquema fatorial 3 x 3 x 2 (tratamentosquímicos x tratamentos físicos x temperaturas), em 4 repeti es de 25 sementes. Os tratamentos: (3) tratamentosquímicos: ácido giberélico (GA3), nitrato de potássio (KNO3) e água; (3) tratamentos físicos: semente intacta,escarificada e sem tegumento; (2) temperatura: 25oC e 30oC. As sementes de crambe descascadas apresentarammaiores freqüências de germina o, em ambas as temperaturas e tratamentos químicos.
Estimativa da área foliar de Crambe abyssinica por discos foliares e por fotos digitais
Toebe, Marcos;Brum, Betania;Lopes, Sidinei José;Cargnelutti Filho, Alberto;Silveira, Tatiani Reis da;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000200036
Abstract: leaf area is important in determining the growth and development of agricultural crops. the aim of this study was to compare the methods of leaf discs and digital photos in estimating leaf area of crambe abyssinica, and model leaf area according to length (c), width (l) and/ or the product of length width (cxl) for different sizes of leaves. for this, in 308 leaves it was determined the leaf area, length, width and the product of length width using the methods of leaf discs and digital photos. then the methods were compared using the linear correlation coefficient between the leaf areas. then, for each method, leaf area (y) depending on the c, of l and the cxl, was modeled through these models: linear, simple linear, quadratic, geometric and exponential. the pearson and spearman linear correlation coefficient between the methods of leaf area and leaf discs of digital photos were 0.9917 and 0.9889, respectively, which shows concordant methods. in both methods, the geometric and quadratic models showed good coefficients of determination of leaf area depending on the length and width of leaves. the leaf width is the variable that best estimates the leaf area. the method of digital photos can be used to estimate crambe's leaf area.
Tamanho de amostra para estima??o do coeficiente de correla??o de Pearson entre caracteres de Crambe abyssinica
Cargnelutti Filho, Alberto;Lopes, Sidinei José;Toebe, Marcos;Silveira, Tatiani Reis da;Schwantes, Ismael Albino;
Revista Ciência Agron?mica , 2011, DOI: 10.1590/S1806-66902011000100019
Abstract: it is important to dimension correctly the sample size, to estimate the pearson correlation coefficient among pairs of characters, with acceptable reliability. the aim of this research was to determine the sample size (number of plants) to estimate the pearson correlation coefficient among 21 characters of crambe (crambe abyssinica hochst). an experiment was conducted in the year 2008. at harvest, 75 plants were selected randomly and measured 21 characters. the measures of central tendency, variability, skewness and kurtosis, were done. normality was verified and investigated the linearity among the characters. the sample size to estimate the pearson correlation coefficient was calculated, for the amplitude of the confidence interval of 95%, equal to: 0.15; 0.20; 0.25; 0.30; 0.35 and 0.40, for each of 210 pairs of characters. for the 210 characters pairs of crambe, the sample size to estimate the pearson correlation coefficient, to the amplitude of the confidence interval of 95% equal to 0.15, ranged from 8 to 665 plants.
Applicability of Moringa oleifera Lam. pie as an adsorbent for removal of heavy metals from waters Aplicabilidade da torta de Moringa oleifera Lam. como adsorvente para remo o de metais pesados de águas  [cached]
Affonso C. Gon?alves Junior,Ana P. Meneghel,Fernanda Rubio,Leonardo Strey
Revista Brasileira de Engenharia Agrícola e Ambiental , 2013,
Abstract: This study evaluated the efficacy of moringa seeds (Moringa oleifera Lam.) as an adsorbent material for removing toxic heavy metals such as cadmium, lead, and chromium from contaminated solutions. The effect of the adsorbent mass was investigated at two pH conditions (5.0 and 7.0). The optimized conditions were 0.300 g of adsorbent at pH 5.0, used for the isotherms construction, and linearized according to Langmuir and Freundlich models. Results showed that cadmium adsorption was similar in both the models used. For lead, the Freundlich model had the best adjustment and chromium was better adjusted by the Langmuir model. It was concluded that the adsorbent was effective in the remediation of solutions containing cadmium, lead and chromium, thus, its use as sustainable alternative material is feasible, since it has low cost, does not need a previous treatment and it is a byproduct. Este estudo objetivou avaliar a eficácia do uso da torta de moringa (Moringa oleifera Lam.) como material adsorvente dos metais pesados tóxicos cádmio (Cd), chumbo (Pb) e cromo (Cr) de solu es contaminadas. Nos testes cinéticos foram variadas as massas do adsorvente em duas condi es de pH (5,0 e 7,0). As condi es otimizadas foram pH 5,0 e massa de 0.300 g de adsorvente, utilizados para a constru o das isotermas e linearizadas conforme os modelos de Langmuir e Freundlich. Realizou-se a determina o dos metais por espectrometria de absor o at mica. Os resultados mostraram que houve semelhan a em ambos os modelos utilizados para a adsor o do Cd. Para o Pb, o modelo de Freundlich apresentou o melhor ajuste e, para o Cr, houve melhor ajuste pelo modelo de Langmuir. Conclui-se, com base nos resultados obtidos, que o adsorvente foi eficaz na remedia o de solu es contendo Cd, Pb e Cr e, assim, é viável a utiliza o desse adsorvente como material alternativo sustentável, pois apresenta baixo custo, n o necessita de tratamento prévio e se trata de um coproduto.
Efficient selection and evaluation of transgenic lines of Crambe abyssinica  [PDF]
Li-Hua Zhu
Frontiers in Plant Science , 2013, DOI: 10.3389/fpls.2013.00162
Abstract: Crambe abyssinica is a dedicated oilseed crop suitable for production of industrial feedstocks. Genetic modification of crambe has progressed substantially in the last few years, but the transformation efficiency needs to be further improved. Meanwhile, developing a reliable molecular system including Southern blot and qRT-PCR analyses is desired for effectively evaluating transgenic lines and gene expression levels of both endogenous and transgenes. In this study, we have developed an efficient transformation protocol with hygromycin as the selective agent for crambe transformation. In the regeneration test, addition of hygromycin at concentration of 5 mg L?1 resulted in 18% of shoot regeneration using crambe hypocotyls as explants, while no regeneration occurred when the hygromycin concentration reached 10 mg L?1. Based on this result, the hygromycin concentration up to 10 mg L?1 was used in the subsequent transformations. The results showed that the transformation efficiency under constant low selection pressure (H3-H3) was similar to that under higher selection pressure first, followed by transfer to lower selection pressure (H10-H3). The PCR, Southern blot and fatty acid composition analyses confirmed the integration of transgenes in the crambe genome. We have also optimized the Southern and qRT-PCR methods for future studies on crambe or related species. For Southern blot analysis on crambe, more than 50 μg DNA is required for a clear band. The choice of enzymes for DNA digestion was not rigid for confirmation of the T-DNA integration, while for determining the copy number of transgenes, suitable enzymes should be chosen. Increasing the enzyme concentration could improve the digestion and 20 μl enzyme was recomended for a complete digestion of up to 80 μg crambe DNA. For qRT-PCR analysis, around 20 days after flowering was observed to be the suitable sampling time for expresseion analysis of genes invovled in the seed oil biosynthesis.
Influence of pre-germination treatments and temperature on the germination of crambe seeds (Crambe abyssinica Hochst)
Deleon Martins,Lima; Pianna Costa,Felipe; Carlos Lopes,José; Nunes Rodrigues,Wagner;
Idesia (Arica) , 2012, DOI: 10.4067/S0718-34292012000300003
Abstract: the objective of this study was to evaluate the influence of pre-germination treatments and temperature on the germination of crambe seed (crambe abyssinica hochst). the completely randomized design was used in a 3 x 3 x 2 factorial (physical treatments x chemical treatments x temperature). the chemical treatments consisted of paper soaked in gibberellic acid (ga3), potassium nitrate (kno3) and water. the physical treatments consisted of maintaining the seed intact, mechanical scarification and seed coat removal. temperatures of 25 and 30 °c were used in the completely randomized design. the variables studied were germination speed index, germination percentage, seed length and seedling dry mass. in the studied conditions, the percentage and rate of germination, length and seedling dry weight of seeds of c. abyssinica hochst were enhanced by removal of the integument, in soaking with ga3 at 25 °c.
Salinity Effects on Development and Productivity of Crambe (Crambe abyssinica) under Greenhouse Conditions  [PDF]
Ana Carolina F. de Vasconcelos, Lúcia Helena G. Chaves, Felipe G. Souza, Hans R. Gheyi, Josely D. Fernandes
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.67091
Abstract: Salinity in soil or water in arid and semi arid regions can severely limit crop production, since the high amount of NaCl contributes to specific ion effects of Cl-, Na+ or both, and to antagonistic effects on nutrient elements. Affected by salt stress, most of the cultivated plants do not fully express their growth potential, which lowers their economic value. Crambe (Crambe abyssinica) is an oil plant of the cruciferous family and it is believed that crambe has great potential to figure as raw material for biofuel; however, literature is not abundant about the effects of salinity in crambe production. This work was carried out in order to evaluate the effects on the development and productivity of crambe irrigated with saline waters under greenhouse conditions. Treatments resulted from the combination of two factors: salinity of irrigation water (ECw) in five levels (1.03-control, 2.5, 4.0, 5.5, and 7.0 dS·m-1) and two types of salts (NaCl and NaCl + CaCl2) with three replications, totaling 30 experimental plots. At the end of the experimental period, soil samples from each plot were collected for chemical and salinity of soil saturation extract analyzes. Salt types did not affect plant parameters. However, the salinity levels presented significant effects on the all plant parameters, decreasing their values with the increase of the salt dose. Salinity levels of the solutions used for irrigation in this study affected the growth of plants and grain yield of crambe. Regarding the type of salts, a higher concentration of Na was observed for NaCl solutions at 2.5, 4.0 and 5.5 dS·m-1 salinity levels.
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