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Contenido de aflatoxinas y proteína en 13 variedades de frijol (Phaseolus vulgaris L.)
Pe?a-Betancourt, Silvia Denise;Conde-Martínez, Víctor;
Revista mexicana de ciencias agrícolas , 2012,
Abstract: in mexico, the bean (phaseolus vulgaris l.) is a highly consumed legume seed (11 kg per capita annually), so that its cultivation it' s quite extensive in different regions. in this study we investigated the presence of aflatoxins in eight common bean varieties and five improved bean varieties, in addition to protein and moisture. in all the varieties tested, the moisture content showed large variations (from 6 to 16%), being 16%of the varieties studied outside the normal (< 12%). the highest content of protein was detected in the improved bean varieties (26.1%) and, the lowest in commercial (19.8% ± 3.09) and wild varieties (20.78% ± 1.93). all of them showed aflatoxins on average of 7.46 ng g-1 and a range from 5 to 13 ng g-1. the highest level of contamination was observed in the improved bean varieties (9.2 ±2.9 ngg-1), followed by the commercial ones ±0.95 7.25 ngg-1 and wild varieties 6 ± 1 ng g-1. tannins were detected in wild bean varieties at a level of 0.44%± 0.13. the results obtained confirm the presence of toxic and anti-nutritional compounds in the different varieties of common and wild beans at levels permitted by national law, but may pose a risk to the consumer's health due to its high consumption.
Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome  [PDF]
Aihua Wang,Yi Ding,Zhenhua Hu,Chufa Lin,Shuzhen Wang,Bingcai Wang,Hongyuan Zhang,Guolin Zhou
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms130911188
Abstract: In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity ( H E) and observed heterozygosity ( H O) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
Regional variability in Phaseolus vulgaris L.  [cached]
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 1977,
Abstract: Within the enlargement of the collections of the Agrobotanical Garden and Herbarium of the Agrobotanical Institute "Dr. P. Groza" Cluj-Napoca, the regional variability of Phaseolus species (P. vulgaris L. and P. multiflorus Lamk.) cultivated in present's plots in different regions of Transylvania (Romania) have been studied, in accordance with the general principles emphasized by national and international organizations (2, 3). Our Phaseolus collection is represented now by voucher specimens field in the Agrobotanical herbarium of the Institute (CLA 25.794-25.919) and more than 1000 seed samples of ecologically adapted (local) populations or recognized agricultural varieties collected for comparation or cultivation. Some of this samples may be placed at the disposal of the interested research workers by Index Seminum Horti agrobotanici Cluj-Napoca, 1977/1978.
Uso de Phaseolus vulgaris y Vigna sinensis como extensores de una bebida láctea fermentada
Granito,Marisela; Trujillo,Lesma; Guerra,Marisa;
Archivos Latinoamericanos de Nutrición , 2004,
Abstract: the objective of this work was to develop a new kind fermented dairy drink, partially substituted with clear varieties of phaseolus vulgaris (caraota) and vigna sinensis (frijol). the formulation of fermented dairy drinks included sterile extracts of caraota and frijol, as partial substitutes which replaced milk: 10, 20 and 30%. the mixtures were inoculated with 2% of a mixture of lactobacillus acidophillus, streptococcus thermophilus y bifidobacterium sp. and were incubated at 42°c for 7 hours. mango and guava jams were used as flavorings at 20%. on the basis of the sensorial evaluation the mixtures 10% frijol- mango, 10% frijol- guava, 30% caraota-mango and 20% caraota- guava were selected. in the selected fermented dairy drinks, the levels of protein, soluble and insoluble fiber, available and resistant starches were increased and the protein digestibility was 81%. the technical feasibility of partial substitution of milk with extracts of phaseolus vulgaris or vigna sinensis for the elaboration of a fermented dairy drink similar to the liquid yogurt kind was demonstrated.
Mirela Campeanu,Cristian Campeanu,Gabriela Capraru,Oana Soiman
Analele ?tiin?ifice Ale Universit??ii Alexandru Ioan Cuza din Ia?i,Sectiunea II A : Genetica si Biologie Moleculara , 2005,
Abstract: Chromosome’s number were 2n=22 on all Phaseolus cultivars. All chromosomes were found morphological uniform, without satellites, and submetacentrics.
Regional variability in Phaseolus vulgaris L. (II.) Seed character frequencies in Transylvania
Attila T. SZABO
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 1985,
Abstract: Attempt was made to evaluate gene frequencies using seed characters registred in Phaseolus vulgaris, cultivated in traditional multicropping (Zea + Phaseolus + Cucurbita) in a sample territory in Transylvania (Romania). The highest frequencies were found in the case of characters determined mostly by I, T, D, and at gene group (according to Prakken 1972); mottled seed coat and violet colour was relatively rare. The short and spherical forms are more frequent, as the long and compressed, with the notable exception of white compressed types. Our knowledge in Phaseolus genetics allowed only a first approach in a very restricted number of characters.
Fraccionamiento de nitrógeno en frijol ( phaseolus vulgaris l.) en el valle de san juan  [cached]
Juan Cedano,Danna de la Rosa,Alfonsina Sánchez,Fernando Oviedo
Agronomía Mesoamericana , 2000,
Abstract: Fraccionamiento de nitrógeno en frijol (Phaseolus vulgaris L.) en el valle de San Juan. Se realizó un estudio para determinar el efecto del fraccionamiento de la fertilización nitrogenada y el momento adecuado para la aplicación de nitrógeno en el cultivo de frijol (Phaseolus vulgaris L.) en cinco localidades del Valle de San Juan, R. D. Los experimentos fueron establecidos del 5 al 14 de noviembre (1997) , se utilizó un dise o de bloques completamente al azar y nueve tratamientos en cada localidad, encontrándose que en los terrenos con altos niveles de nitrógeno no hubo respuesta a la aplicación de nitrógeno ni al fraccionamiento de este nutrimento; mientras que en los suelos con deficiencia en nitrógeno si hubo respuesta a la fertilización nitrogenada encontrándose diferencia estadística significativa a la aplicación y al momento de aplicación del fertilizante. Entre las localidades hubo diferencia estadística significativa (P>0.05), mientras que no se encontró interacción entre los tratamientos y las localidades
Study of the early abortion in reciprocal crosses between Phaseolus vulgaris L. and Phaseolus polyanthus Greenm
Geerts P.,Toussaint A.,Mergeai G.,Baudoin J.P.
Biotechnologie, Agronomie, Société et Environnement , 2002,
Abstract: The causes of early embryo abortion in the reciprocal crosses between Phaseolus vulgaris L. and Phaseolus polyanthus Greenm. were studied. Methacrylate resin sections, 2 micron meter thick, of 2 to 6 day-old hybrid seeds were used to examine the stage of embryo development and the state of seed tissues. These observations permitted the determination of the main causes of abortion and the developmental stages at which interspecific embryos should be rescued. Early nutritional barriers in Phaseolus polyanthus (Q) x Phaseolus vulgaris crosses are related to a deficient endosperm development while in reciprocal crosses, endothelium proliferation and, in some extent, hypertrophy of the vascular elements might be the main causes of early embryo abortion. The importance of the abnormalities observed during embryo development depended to a great extent on the compatibility between the genotypes crossed. Results also suggest that the appropriate time for embryo rescue when Phaseolus polyanthus is a female partner is at the early globular stage.
Embryogenèse précoce comparative lors des croisements entre Phaseolus coccineus L. et Phaseolus vulgaris L.  [PDF]
Pamphile Nguema Ndoutoumou,André Toussaint,Jean-Pierre Baudoin
Biotechnologie, Agronomie, Société et Environnement , 2007,
Abstract: Comparative early embryogenesis in crossings between Phaseolus coccineus L. and Phaseolus vulgaris L. Theinterspecifi c hybridization between Phaseolus coccineus L. and Phaseolus vulgaris L. is useful for the genetic improvementof the common bean. The use of the P. vulgaris cytoplasm for such hybridizations leads usually to a rather fast return tothe maternal form in the subsequent generations. When P. vulgaris is the pollinator, crosses result in early embryo abortion(globular or heart-shaped embryos). A competition between the endosperm and the embryo, on the one hand, and between thesuspensor and the embryo, on the other hand, could generate diffi culties of feeding young embryos. Histological sections usingthe 2-Hydroxyethyl Methacrylate (HEMA) resin method on the embryos of P. coccineus (NI16) and P. vulgaris (NI637 andX707) genotypes, as well as on their genotypic combination (NI16 × NI637 and NI16 × X707 and reciprocal crosses) enableus to explain partially these abortion cases. Observations concern embryos from 3 to 6 days after pollination (DAP). Embryodevelopment (suspensor and embryo proper) of the hybrids is slower than that of the parents whatever the crossing. Ingrowthsof suspensor basal cells observed when P. coccineus is the maternal parent are characteristic of the presence of the cytoplasmof these species. Endothelium deterioration (or proliferation) in hybrid embryos would rather be related to the degree reachedby the process of abortion in the embryo concerned.
Journal of Central European Agriculture , 2006,
Abstract: The genetic diversity of landraces is thought to be the economic valuable part of global biodiversity and is considered of paramount importance for future world production. The investigation was performed on 30 common bean landraces (Ph. vulgaris L.) from different geographic origin of Portugal and Bulgaria. The morphological characterization was done according to the IPGRI descriptors (Rome, Italy). Twenty morphological traits were studied in Portuguese and Bulgarian landraces of common bean (Phaseolus vulgaris L.). Accessions number 99E059(BG), 99E0123(BG), PH2(PT) and PH23(PT) are of special interest for breeding purposes.
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