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The Value of Sedimentation Test in the Prediction of Breadmaking Quality of Wheat and Determination of its Variability Through Analysis of Glutenin Subunits by RP-HPLC  [cached]
R. Amiri,A. Rezai
Journal of Science and Technology of Agriculture and Natural Resources , 2001,
Abstract: In order to evaluate the relationship between SDS-sedimentation value and breadmaking quality of wheat (Triticum aestivum L.), glutenin subunits of different genotypes (foreign and Iranian cultivars) were analyzed by reverse-phase high performance liquid chromatography (RP-HPLC). SDS-sedimentation value was used as an indirect criterion for breadmaking quality. Correlation coefficients revealed a closer relationship between low molecular weight (LMW) glutenin subunits and variation in SDS-sedimentation value. Principal component analysis confirmed the presence of association between some of the glutenin subunits and SDS-sedimentation value. Based on the stepwise regression analysis, two LMW and four high molecular weight (HMW) peaks (subunits) were selected which accounted for 70.2 and 18.7% of variability in SDS sedimentation values, respectively. On the basis of the results of the stepwise regression analysis, a discriminant function was developed. The great efficiency of discriminant function in correct classification of completely different genotypes (Iranian landraces and cultivars) showed that the observed relationship between glutenin subunits and SDS-sedimentation value has a genetic basis and the effects of LMW and HMW glutenin subunits on SDS-sedimentation value are additive. Therefore, it seems that this method based on more protein components (rather than only on HMW glutenin subunits) can be used to predict breadmaking quality of wheat against many genetic backgrounds.
Evaluation of Grain Quality Traits, Glutenin Subunits and Their Relationship in Durum Wheat  [cached]
M. Golabadi,A. Arzani
Journal of Science and Technology of Agriculture and Natural Resources , 2002,
Abstract: To study grain quality traits and their relationships with high molecular weight (HMW) and low moleculor weight (LMW) glutenin subunits, 104 durum wheat genotypes were used. Six grain quality characteristics comprising wet and dry gluten content, test weight, grain hardiness, protein content and SDS sedimentation volume were studied. HMW and LMW glutenin subunits were evaluated using SDS-polyacrylamid gel electrophoresis (SDS-PAGE) in 33 genotypes. Statistical analysis including correlation coefficients, factor analysis, cluster analysis of genotypes based on qualitative traits, analysis of variance in qualitative traits based on HMW, LMW, and combination of subunits as well as all of subunits, and canonical correlation analysis between glutenin subunits and qualitative traits were used. Factor analysis of the genotypes detected 2 factors, which explained 65 percent of the total variation among the data. These were named quantitative protein and qualitative protein. Based on cluster analysis, the genotypes were classified into four clusters. The genotypes in groups 2 and 4 were beneficial in terms of protein quantity and quality. In evaluating glutenin subunits, 7 HMW subunits and 2 LMW subunits were detected. Subunit null of Glu-Al was observed. Subunits 7+8, 6+8, 13+16 and 20 at Glu-Bl were observed; however, neither of them had significant differences in qualitative traits, but 6+8 and 7+8 had a higher SDS sedimentation rate than 20. LMW-1 and LMW-2 had significant differences in protein content and SDS sedimentation; LMW-1 had higher protein content whereas LMW-2 had higher SDS sedimentation. Combibation of subunit LMW-1 / HMW 7+8 had the highest protein content and LMW-2/HMW 7+8 had the highest SDS sedimentation. Results of canonical correlation showed that presence of LMW-2 and HMW 7+8, and absence of LMW-1 and HMW 20 in durum wheats caused an increase in SDS sedimentation and a reduction in protein and gluten content.
Characterization and glutenin diversity in tetraploid wheat varieties in Sulaimanyah by wheat storage proteins
NA Tahir
African Journal of Biotechnology , 2008,
Abstract: Important methods applied for the breeding of bread-quality wheat (Triticum durum L.) consist of smallscale bread-quality tests for the determination of the grain protein content, SDS-sedimentation volume, thousand weight kernel and kernel diameter. Wheat grains of six varieties were analyzed. The thousand weight kernel, protein content, kernel diameter, SDS-sedimentation volume and SDS index showed a difference significant among the varieties, whereas the flour yield showed no difference significant. The quality score and the variability of seed storage-proteins were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The high molecular weight (HMW) and low molecular weight (LMW) glutenin subunit band patterns for each variety were assigned the corresponding Payne numbers and theoretical quality scores based on those assignments. Three patterns of HMS-GS are designed: 6+8, 7+8 and 8+20. On the other hand, two patterns of LMW-GS were detected in the durum varieties. Genetic diversity of wheat was evaluated by constructing the dendrogram for high molecular weight (HMW) and low molecular weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in a developing country.
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat
Xiaodong Zhang,Rongqi Liang,Xüqing Chen,Fengping Yang,Liquan Zhang
Chinese Science Bulletin , 2003, DOI: 10.1007/BF03187050
Abstract: The expression vector pBPC30, which carries the high molecular weight glutenin subunit (HMW-GS)1Dx5 and1Dy10 genes, was transferred into hexaploid winter wheat cv. Jinghua No. 1, Jing411 and Jingdong No. 6 explants of immature embryos and immature inflorescence by particle bombardment. A. large number of resistant transgenic plants were obtained under the selection of herbicide bialaphos or phosphinothricin (PPT). Confirmed transgenic plants of T0 generation showed successful integration of HMW-GS genes and bar gene into the wheat genome. T1 generation of transgenic plants can resist 20–150 mg/L PPT. Protein analysis of T2 seed by SDS-PAGE showed that HMW-GS1Dx5 and1Dy10 genes were well expressed in offspring seed of transgenic lines by co-expression with or substitution of endogenous1Dx2 or1Dy10. In one transgenic line, TG3-74, a new protein band between endogenous protein subunits 7 and 8 (marked as 8*) of glutenin appeared, but endogenous subunit 8 (encoded by lBy8 gene) was absent. Analysis of gluten rheological quality on seed proteins of 102 T3 plants showed that the sedimentation value of 5 transgenic lines (44.2–49.0 mL) was remarkably improved, 59.6%–64.3% higher than that of wild type Jinghua No. 1 and Jingdong No. 6, similar to bread wheat Cheyenne (48.0 mL). Analysis of dough rheological properties of transgenic lines showed that the dough stable time of 5 transgenic lines range from 16 to 30 min, whereas the dough stable time of wild type was only between 3–7 min. Our research suggests that introducing novel HMW-GS genes into wheat is an efficient way to improve its bread-making quality.
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS) genes in winter wheat
Transgene inheritance and quality improvement by expressing novel HMW glutenin subunit (HMW-GS)genes in winter wheat

ZHANG Xiaodong,LIANG Rongqi,YANG Fengping,Zhang Liquan,
CHEN
,Xüqing

科学通报(英文版) , 2003,
Abstract: The expression vector pBPC30, which carries the high molecular weight glutenin subunit (HMW-GS) 1Dx5 and 1Dy10 genes, was transferred into hexaploid winter wheat cv. Jinghua No. 1, Jing411 and Jingdong No. 6 ex-plants of immature embryos and immature inflorescence by particle bombardment. A large number of resistant trans-genic plants were obtained under the selection of herbicide bialaphos or phosphinothricin (PPT). Confirmed transgenic plants of T0 generation showed successful integration of HMW-GS genes and bar gene into the wheat genome. T1 generation of transgenic plants can resist 20150 mg/L PPT. Protein analysis of T2 seed by SDS-PAGE showed that HMW-GS 1Dx5 and 1Dy10 genes were well expressed in offspring seed of transgenic lines by co-expression with or substitution of endogenous 1Dx2 or 1Dy10. In one transgenic line, TG3-74, a new protein band between endogenous pro-tein subunits 7 and 8 (marked as 8*) of glutenin appeared, but endogenous subunit 8 (encoded by 1By8 gene) was ab-sent. Analysis of gluten rheological quality on seed proteins of 102 T3 plants showed that the sedimentation value of 5 transgenic lines (44.249.0 mL) was remarkably improved, 59.6%64.3% higher than that of wild type Jinghua No. 1 and Jingdong No. 6, similar to bread wheat Cheyenne (48.0 mL). Analysis of dough rheological properties of transgenic lines showed that the dough stable time of 5 transgenic lines range from 16 to 30 min, whereas the dough stable time of wild type was only between 37 min. Our research suggests that introducing novel HMW-GS genes into wheat is an effi-cient way to improve its bread-making quality.
Allelic variation of HMW glutenin subunits of Ethiopian bread wheat cultivars and their quality
T Dessalegn, CS Van Deventer, MT Labuschagne, H Martens
African Crop Science Journal , 2011,
Abstract: High molecular weight glutenins are often effective in identifying wheat (Triticum aestivum) genotypes with good baking quality. The high molecular weight glutenin subunit composition of Ethiopian cultivars and advanced lines was investigated to determine their influence on quality. Three alleles at Glu-A1, five at Glu-B1 and two at Glu-D1 and eleven different banding patterns were identified. Few cultivars had biotypes at Glu-A1 and Glu-D1 loci. There were highly significant differences between genotypes and banding patterns for the SDS-sedimentation test, mixograph development time, alveograph strength and loaf volume; but not for protein content. The frequency of subunits 5+10 among genotypes was 73%. The accumulation of high scoring alleles in our germplasm was without deliberate selection pressure towards high Glu-1 scoring alleles during breeding. Introductions from CIMMYT are widely used and such materials might have gone through selection and accumulation of such alleles at CIMMYT. Most of the studied materials had the required subunits of high quality scores and better values of the quality traits than originally thought. Therefore, the concern about poor baking quality is unlikely to be due to Glu-1 allelic variations alone. Key Words: Allelic variation, grain quality, HMW glutenins
HMW glutenin subunits in multiploidAegilops species: composition analysis and molecular cloning of coding sequences
Ruili Xie,Yongfang Wan,Yan Zhang,Daowen Wang
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187192
Abstract: TheAegilops genus contains species closely related to wheat. In common with wheat,Aegilops species accumulate high molecular weight (HMW) glutenin subunits in their endospermic tissue. In this study, we investigated the composition of HMW glutenin subunits in four multiploidAegilops species using SDS-PAGE analysis. Furthermore, by working withAe. ventricosa, we established an efficient genomic PCR condition for simultaneous amplification of DNA sequences coding for either x-or y-type HMW glutenin subunits from polyploidAegilops species. Using the genomic PCR condition, we amplified and subsequently cloned two DNA fragments that may code for HMW glutenin subunits inAe. ventricosa. Based on an analysis of the deduced amino acid sequences, we concluded that the two cloned sequences encode one x- and one y-type of HMW glutenin subunit, respectively.
HMW glutenin subunits in multiploid Aegilops species: composition analysis and molecular cloning of coding sequences

XIE Ruili,WAN Yongfang,ZHANG Yan,WANG Daowen,

科学通报(英文版) , 2001,
Abstract: The Aegilops genus contains species closely related to wheat. In common with wheat, Aegilops species accumulate high molecular weight (HMW) glutenin subunits in their endospermic tissue. In this study, we investigated the composition of HMW glutenin subunits in four multiploid Aegilops species using SDS-PAGE analysis. Furthermore, by working with Ae. ventricosa, we established an efficient genomic PCR condition for simultaneous amplification of DNA sequences coding for either x- or y-type HMW glutenin subunits from polyploid Aegilops species. Using the genomic PCR condition, we amplified and subsequently cloned two DNA fragments that may code for HMW glutenin subunits in Ae. ventricosa. Based on an analysis of the deduced amino acid sequences, we concluded that the two cloned sequences encode one x- and one y-type of HMW glutenin subunit, respectively.
Correlation between high molecular weight gluten subunits composition and bread-making quality in Brazilian wheat
Schuster, Ivan;Souza, Moacil Alves de;Cardoso, Ant?nio Américo;Sediyama, Carlos Sigueyuki;Moreira, Maurílio Alves;
Brazilian Journal of Genetics , 1997, DOI: 10.1590/S0100-84551997000400019
Abstract: bread-making quality is one of the most important targets in the genetic improvement of wheat. although extensive analyses of quality traits such as farinography, sodium dodecyl sulfate (sds) sedimentation, alveography, and baking are made in breeding programs, these analyses require high amounts of seeds which are obtained only in late generations. in this experiment the statistical correlations between the high molecular weight subunit of glutenin and bread-making quality measured by alveograph, farinograph and sds sedimentation were evaluated. seventeen wheat genotypes were grown under the same conditions, each producing about 1 kg of seeds for the evaluations. the high molecular weight (hmw) glutenin subunits were analyzed by sds-page. statistical correlations were highly significant between hmw glutenin subunits and alveograph and sds sedimentation. these results indicate the possibility of manipulating major genes for wheat seed quality by coupling traditional breeding with non-destructive single seed analysis. only half seed is necessary to perform the sds-page analysis. therefore, the other half seed can be planted to generate the progeny. seed yield and sds sedimentation were statistically correlated, indicating the possibility of simultaneous selection for both traits
Correlation between high molecular weight gluten subunits composition and bread-making quality in Brazilian wheat  [cached]
Schuster Ivan,Souza Moacil Alves de,Cardoso Ant?nio Américo,Sediyama Carlos Sigueyuki
Brazilian Journal of Genetics , 1997,
Abstract: Bread-making quality is one of the most important targets in the genetic improvement of wheat. Although extensive analyses of quality traits such as farinography, sodium dodecyl sulfate (SDS) sedimentation, alveography, and baking are made in breeding programs, these analyses require high amounts of seeds which are obtained only in late generations. In this experiment the statistical correlations between the high molecular weight subunit of glutenin and bread-making quality measured by alveograph, farinograph and SDS sedimentation were evaluated. Seventeen wheat genotypes were grown under the same conditions, each producing about 1 kg of seeds for the evaluations. The high molecular weight (HMW) glutenin subunits were analyzed by SDS-PAGE. Statistical correlations were highly significant between HMW glutenin subunits and alveograph and SDS sedimentation. These results indicate the possibility of manipulating major genes for wheat seed quality by coupling traditional breeding with non-destructive single seed analysis. Only half seed is necessary to perform the SDS-PAGE analysis. Therefore, the other half seed can be planted to generate the progeny. Seed yield and SDS sedimentation were statistically correlated, indicating the possibility of simultaneous selection for both traits
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