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Biolistic Transformation of Citrullus vulgaris Schrad (Watermelon)  [PDF]
F. Suratman,F. Huyop,A. Wagiran,Z. Rahmat
Biotechnology , 2010,
Abstract: In this study, DNA-coated gold particles were used to transform cotyledon sections including axillary meristem of Citrullus vulgaris using the biolistic method for optimizing the transformation parameters and eventually regeneration of putative transgenic watermelon plants. Initially, five biolistic parameters namely helium pressure, macrocarrier to target tissue distance, explant ages, DNA concentrations and osmotic treatment were optimized using plasmid pRQ6 (carrying both gusA and hpt genes). The optimized biolistic parameters for cotyledon sections of Citrullus vulgaris explants were determined as follow: 1100 psi helium pressure, 6 cm target distance, 4-day-old explants, concentration of DNA at 1.2 μg/bombardment and osmotic treatment for 24 h on medium supplemented with 0.6 M mannitol prior to bombardment. Using the optimized parameters, transformation of watermelon cotyledons was carried out using pAHG11, pCAMBIA 1301, co-transformation (pAHG11+pCAMBIA 1301) and pCambar plasmids. Bombarded tissue’s were selected on either 5 mg L-1 hygromycin or 2 mg L-1 PPT. Finally, the transformants produced were subjected to GUS histochemical assay. Integration of the transgenes into transgenic watermelon genome was confirmed by PCR analysis.
Efecto del AIB y el TDZ en el enraizamiento in vitro de plantas de Bambusa vulgaris var vulgaris Schrad. ex Wendl  [cached]
García Ramírez Yudith,Freire-Seijo Marisol,Pérez Mederos Blanca Rosa,Hurtado Rivalta Ortelio
Revista Colombiana de Biotecnología , 2012,
Abstract: Título en ingles: Effect of IBA and TDZ on in vitro rooting of plants Bambusa vulgaris var. vulgaris Schrad. ex Wendl. Resumen: Bambusa vulgaris var. vulgaris Schard. ex Wendl. sobresale dentro del género por sus propiedades físico-mecánicas y por el tama o de sus culmos. Desarrollar la propagación vía organogénesis sería una alternativa para propagar esta especie. Sin embargo, los bajos porcentajes de enraizamiento y de superviviencia ex vitro han sido elementos que han afectado la propagación de diferentes especies de bambúes. Con el objetivo de determinar el efecto de diferentes concentraciones de AIB y TDZ en la emisión de raíces in vitro, se evaluó el número de plantas con raíces, la altura de las plantas (cm) desde la base hasta el punto de inserción de la primera hoja y el largo de la raíz (cm). Los resultados demostraron que tanto el AIB como el TDZ influyeron en el enraizamiento in vitro de Bambusa vulgaris var vulgaris. Al adicionar al medio de cultivo TDZ (0,6 mg.L-1) se obtuvieron los mayores porcentajes de formación de raíces (88,2%) y estas fueron emitidas mostrando un geotropismo positivo. Palabras clave: bambú; cultivo de tejidos; reguladores de crecimiento. Abstract: Bambusa vulgaris var. vulgaris Schard. ex Wendl. stands out into the genus by its physic - mechanical properties and the culms size. Propagation via organogenesis would be an alternative to propagate this species. However, the low percentages of rooting and ex vitro survival have been elements that have affected the propagation of different bamboo species. The number of plants with roots, plant height (cm) (from the base to the insertion point of the first leaf) and root length (cm) were evaluated in order to determine the effect of different concentrations of IBA and TDZ in the emission of roots in vitro. Results demonstrated that both, AIB and TDZ, influenced the in vitro rooting of Bambusa vulgaris var vulgaris. Greater rooting percentages (88.2%) were obtained by adding TDZ to the culture medium (0.6 mg.L-1). Besides, they showed a positive geotropism. Keywords: bamboo; tissue culture; plant growth regulators.
In vitro Shoot Regeneration of Citrullus vulgaris Schrad (Watermelon)  [PDF]
F. Suratman,F. Huyop,G.K.A. Parveez
Biotechnology , 2009,
Abstract: In this study, shoot regeneration using cotyledons derived from seedlings of diploid and triploid yellow watermelon (cultivars Hwang Fong Yellow Queen, Round Dragon and Chin San Seedless) was investigated. Multiple shoots from auxiliary meristems were obtained without adventitious shoot. Shoot regeneration system for watermelon was successfully established from cotyledon sections of 4 to 5 day-old in vitro seedling. The explants were collected from the proximal cotyledon with hypocotyls segment. Highest mean number of multiple shoots were obtained (9.83±0.54) for cultivar Hwang Fong Yellow Queen on MS medium supplemented with 20 μM BAP as compared to (8.87±0.81) for Chin San Seedless on 5 μM of BAP and (6.00±0.32) for Round Dragon on 10 μM of BAP. From these results, cultivar Hwang Fong Yellow Queen was used for further experiment due to its highest percentage of germination and mean number of shoots. Subsequently, the cultivar successfully rooted (80%) on half strength MS medium supplemented with 0.5 μM IAA. Finally, 50% of these rooting plantlets were acclimatized on soil.
Efecto del AIB y el TDZ en el enraizamiento in vitro de plantas de Bambusa vulgaris var vulgaris Schrad. ex Wendl.
García Ramírez,Yudith; Freire-Seijo,Marisol; Pérez Mederos,Blanca Rosa; Hurtado Rivalta,Ortelio;
Revista Colombiana de Biotecnología , 2012,
Abstract: bambusa vulgaris var. vulgaris schard. ex wendl. stands out into the genus by its physic - mechanical properties and the culms size. propagation via organogenesis would be an alternative to propagate this species. however, the low percentages of rooting and ex vitro survival have been elements that have affected the propagation of different bamboo species. the number of plants with roots, plant height (cm) (from the base to the insertion point of the first leaf) and root length (cm) were evaluated in order to determine the effect of different concentrations of iba and tdz in the emission of roots in vitro . results demonstrated that both, aib and tdz, influenced the in vitro rooting of bambusa vulgaris var vulgaris . greater rooting percentages (88.2%) were obtained by adding tdz to the culture medium (0.6 mg.l -1 ). besides, they showed a positive geotropism.
Comparative Study of Amino Acid and Proximate Composition of Citurllus colocynthis and Citrullus vulgaris Seeds  [PDF]
J.O. Ogundele,A.A. Oshodi,I.A. Amoo
Pakistan Journal of Nutrition , 2012,
Abstract: Citrullus colocynthis and Citrullus vulgaris both look alike and belong to the genus Citrullus except that Citrullus colocynthis has thick black ring round its circumference. However, they are among the underutilized seeds of the World. Citrullus colocynthis and Citrullus vulgaris seed flours were analyzed for their amino acid and proximate compositions which was further subjected to statistical analysis to determine the correlation between the proximate compositions of the two species. The seeds show significant difference in fat and protein contents, but they are both good sources of fats and protein with values (56.00 and 49.59) % fat and (24.37 and 32.96)% protein contents for Citrullus colocynthis and Citrullus vulgaris respectively. However, they are closely related in fibre (1.91 and 2.00)%, moisture (3.08 and 2.75)% and ash contents (3.15 and 3.53)% respectively. The percentage essential amino acids of the seeds are also closely related: Citrullus colocynthis (52.73) and Citrullus vulgaris (50.86). The biological values of Citrullus colocynthis and Citrullus vulgaris are significantly different (12.83 and 26.35) with Protein efficiency ratios that are closely related (2.71 and 2.58) respectively.
Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract
Sunil Kumar, Dinesh Kumar, Man Jusha, Kamal Saroha, Nidhan Singh, Bhoodev Vashishta
Acta Pharmaceutica , 2008, DOI: 10.2478/v10007-008-0008-1
Abstract: Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Preliminary phytochemical screening of the plant showed the presence of large amounts of phenolics and flavonoids. Subsequent quantification showed the presence of 0.74% (m/m) phenolics (calculated as gallic acid) and 0.13% (m/m) flavonoids calculated as catechin equivalents per 100 g of fresh mass. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. In the present study, methanolic fruit extract of C. colocynthis was screened to evaluate its freeradical scavenging effect. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500 μg mL-1.
Celulose monossulfito a partir de Bambusa vulgaris schrad
Azzini, Anísio;Nagai, Violeta;Ciamarello, Dirceu;
Bragantia , 1979, DOI: 10.1590/S0006-87051979000100014
Abstract: pulps in laboratory conditions were obtained from bambusa vulgaris schrad by the alkaline monosulphite process, with various concentrations of the cooking chemicals. the strength properties of these pulps were compared to those obtained by the sulphate process. the results showed that both pulps were similar, excepting the tear resistance that was higher in the sulphate one. it was observed that chips with 5.5 x 0.8 x 0.5cm, respectively to the length, width and thickness were appropriated to the sulphate process but not to the conditions of the alkaline monosulphate process, which produced pulps with low values in terms of screening yield, percentage of screenings and kappa number. the variability of bamboo culm in the radial direction was also determined regarding basic density and fiber dimensions. the results showed variations in those properties, mainly in the basic density values which sharply decreased from the outside to the inside parts of the bamboo culm.
INDUCTION OF PLANTLET FROM FLORAL BUD OF WATERMELON (CITRULLUS VULGARIS SCHRAD) IN VITRO

科学通报(英文版) , 1980,
Abstract:
Cotyledon with Hypocotyl Segment as an Explant for the Production of Transgenic Citrullus vulgaris Schrad (Watermelon) Mediated by Agrobacterium tumefaciens
F. Suratman,F. Huyop,A. Wagiran,Z. Rahmat
Biotechnology , 2010,
Abstract: In this study, we describe the successful production of transgenic watermelon using Agrobacterium tumefaciens after optimizing a couple of transformation parameters using cotyledon with hypocotyl segment from 5 day old in vitro seedlings as target tissues. It was found that the best condition for transformation of watermelon was using bacteria at a concentration of OD600 0.6, inoculation for 30 min and 3 day of co-cultivation. Addition of 200 μM acetosyringone in the medium helped to further increase the transformation efficiency. Agrobacterium tumefaciens strain EHA101 was found to be more effective to infect this watermelon variety as compared to LBA 4404 strain. Wounding of the explant meristems to induce multiple shoot was best carried out after 3 day. Transformed explants were selected on medium containing 5 mg L-1 hygromycin. A total of 31 putative transformed plants were recovered with 25 shoots were found to be GUS positive after transformation of 110 cotyledons. A total of 15 GUS positive shoots amplified the hpt gene using PCR. However, only 7 shoots were successfully regenerated into whole plants.
Physical Characteristics and Anatomical Properties of Cultivated Bamboo (Bambusa vulgaris Schrad.) Culms  [PDF]
R. Wahab,A. Mohamed,M.T. Mustafa,A. Hassan
Journal of Biological Sciences , 2009,
Abstract: Two age-groups (2 and 4 years old culms) of cultivated Bambusa vulgaris Schrad. were harvested and investigated for their physical characteristics and anatomical properties. The physical characteristics did not show significant differences between both the 2 and 4 year-old bamboo. However, variation in anatomical properties was observed between the two age-group bamboos. The anatomical structure in bamboo has a very strong correlation with the age, location where the samples were taken, moisture content and the basic density. The basic density is higher in the 4 year-old culms than in the 2 year-old by 5 to 8% and increases from lower to upper internodes showing that there is a maturation process going on between the two age-groups relative to the two of tissue types. The frequency of vascular bundles is greater at the bottom and top portion than in the middle portion of both age-groups. There was no difference in vessel diameter between the 2 and 4 year-old culms at the middle of the culms wall thickness. The cell wall thickness of both parenchyma and fibre were greater in the 4 year-old than in the 2 year-old culms.
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