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Estudio Preliminar de las Propiedades de la Semilla de Limón Mexicano (Citrus aurantifolia swingle) para su Posible Aprovechamiento
Arriola-Guevara,Enrique; García-Herrera,Tania; Guatemala-Morales,Guadalupe M; García-Fajardo,Jorge A;
Información tecnológica , 2006, DOI: 10.4067/S0718-07642006000600015
Abstract: key properties of seed of mexican lime (citrus aurantifolia swingle) were studied for potential uses by the food industry. characteristics considered included physical properties, composition, toxicity to rodents (average lethal dose and chronic toxicity), as well as germicidal activity (inhibition rings and germicidal effectiveness). the seed contained 21% protein, 39% vegetable oil and 29% fiber, particularly lignin. the seed could potentially be used as a basic component for nutritional supplements or functional food products. the toxicity was nil, and thus seed derivatives were safe for human consumption. some germicidal effectiveness was found against candida albicans, 85.77% (starting population = 0.304*108 ufc/ml), and against escherichia coli, 91.447% (starting population = 190*108 ufc/ml), suggesting seed extracts could be used as an ingredient in germicidal products.
Structural and Developmental Studies on Oil Producing Reproductive Organs in Lime (Citrus aurantifolia Swingle)  [PDF]
Maryam Rafiei,Homa Rajaei
Journal of Biological Sciences , 2007,
Abstract: Anatomical changes in lime (Citrus aurantifolia Swingle) reproductive organs were investigated, with emphasis on the ontogeny of essential oil glands and their relation to organ development. Perianth had a leaf-like internal structure, with the developmental changes restricted to the ground system. Ovary diameter increased by cell divisions until fruit color change and by increase in size, wall thickness and intercellular space towards fruit maturity. Development of oil glands in flower and fruit peel revealed a similar pattern and was investigated in the ovary wall. Glands seemed to develop from some epidermal and subepidermal cells, into a conical stalk and a globular or oblong structure consisting of a central cavity surrounded by a protective sheath. Initiation of ovary oil glands started at preanthesis and was restricted to young green fruit. Mature oil glands continued to enlarge throughout fruit growth. Disputes regarding the manner of cavity opening in Citrus could be resolved by considering the three dimensional aspect of the oil glands.
Agrobacterium-Mediated Transformation of Mexican Lime (Citrus aurantifolia Swingle) Using Optimized Systems for Epicotyls and Cotyledons  [PDF]
Maria Luiza P. de Oliveira, Gloria Moore, James G. Thomson, Ed Stover
Advances in Bioscience and Biotechnology (ABB) , 2015, DOI: 10.4236/abb.2015.611069
Abstract: Transgenic Mexican lime (Citrus aurantifolia Swingle) was produced through two explant sources, each using systems previously optimized for each source. One used epicotyls segments, which was the predominant explant for transgenic Citrus production following co-cultivation with Agrobacterium, and has a well-established protocol. The other procedure used embryo cotyledons from mature seeds, which was developed in our lab as an alternative for stable Citrus transformation. Cotyledon transformation and regeneration protocols were optimized by comparing variables in culture medium composition on shoot regeneration and four parameters in transient transformation. The optimized protocols were compared, and frequency of regeneration, frequency of transgenic plant-recovery and stable transformation efficiency indicated the superiority of the cotyledon protocol for Agrobacterium-mediated genetic transformation in Mexican lime. The tissue choice resulted in marked improvement in shoot regeneration (14.1% of explants producing shoots in epicotyls; 55.8% in cotyledons), stable transformation frequency (11.4% of epicotyls explants; 40.2% in cotyledons), and frequency of transgenic plant-recovery (37.9% in epicotyl explants; 92.6% in cotyledons). Thus, easy availability of explants using embryo cotyledons from mature seeds, technical simplicity, shortening of transformation time-course, and higher transformation and regeneration frequencies makes this new system an attractive alternative over the previously published Citrus transformation protocols. In the course of this project, we generated Mexican lime with a Recombinase Mediated Exchange Cassette landing pad, which was designed for stacking transgenes.
Acute and subchronic toxicity study of the water extract from root of Citrus aurantifolia (Christm. et Panz.) Swingle in rats
Siharat Chunlaratthanaphorn,Nirush Lertprasertsuke,Umarat Srisawat,Amornnat Thuppia
Songklanakarin Journal of Science and Technology , 2007,
Abstract: Acute and subchronic toxicities of the water extract from the roots of Citrus aurantifolia were studied in both male and female rats. Oral administration of the extract at a single dose of 5,000 mg/kg body weight (5 male, 5 female) did not produce signs of toxicity, behavioral changes, mortality or differences on gross appearance of internal organs. The subchronic toxicity was determined by oral feeding the test substance at the doses of 300, 600 and 1,200 mg/kg body weight for 90 days (10 male, 10 female). The examinations of signs, animal behavior and health monitoring showed no signs of abnormalities in the test groups as compared to the controls. The test and control groups (on the 90th day) and the satellite group (on the 118th day) were analyzed by measuring their final body and organ weights, taking necropsy, and examining hematological parameters, blood clinical chemistry and histopathology features. The oral administration of 1,200 mg/kg/ day of the extract of C. aurantifolia in male and female rats caused a significant increase in the liver enzymes, which remained within the normal range, but did not produce a significant histopathological change in the internal organs. In conclusion, the extract from the roots of C. aurantifolia administered orally did not cause acute or subchronic toxicities to male and female rats.
Comportamiento de dos cultivares de limón mexicano [Citrus aurantifolia (Christm) Swingle] en portainjertos desarrollados en suelos con dos profundidades
Medina-Urrutia, V. M.;Robles-González, M. M.;Velázquez-Monreal, J. J.;
Revista Chapingo. Serie horticultura , 2009,
Abstract: the objective of this research was to assess the growth, yield and fruit quality of the cultivars mexican lime with thorns ('mce') and mexican thornless ('mse') [citrus aurantifolia (christm) swingle] grafted on to different vigorous rootstocks established in soils with two different depths. two different sites were chosen to set up the experiments, one with shallow soil (ss) with a water table 80 to 120 cm deep, and the other with deep soil (sp). rootstocks evaluated on ss site were macrophylla (citrus macrophylla wester), rough lemon (c. jambhir lush), taiwanica (c. taiwanica) troyer citrange (poncirus trifoliate x c. sinensis), and sour orange (c. aurantium). the same rootstocks, except for troyer citrange and sour orange, were used in s p. 'mce' trees had larger crown diameter and yield than 'mse' in both soils. of all rootstocks, macrophylla supported the largest tree crown diameter and average yield of the two lime cultivars, in both soil conditions. however, this rootstock was similar to taiwanica and rough lemon in tree height and crown volume. fruit quality was not affected by the rootstocks used. soil depth affected tree performance of most scion/rootstock combinations throughout the years. on the ss site, 'mce' was more productive than 'mse' on all rootstocks during the six years of production; however tree growth and yield of most combinations were affected by the water table. on the sp site, mexican thornless lime trees on macrophylla and rough lemon were as vigorous and productive as 'mce' on the same rootstocks. the most promising combinations for the ss site were' mce'/macrophylla and 'mce'/troyer citrange. on the sp site, the best combinations were both 'mce' and 'mse' on macrophylla rootstock.
Comparación del "Tabog" (Swinglea glutinosa Merr) con el limón "rugoso" (Citrus jambhiri Swingle) y la mandarina "Cleopatra" (C. reticulata Swingle) como porta-injerto para la lima acida ''Tahiti" (C. aurantifolia Swingle)  [cached]
Restrepo C. Orlando,Torres M. Rodrigo,Salazar C. Raúl
Acta Agronómica , 1987,
Abstract: At the C.N.I.-I.C.A. Palmira, fruit trees program the influence of "Tabog" Swinglea glutinosa was evaluated as rootstock for acid lime "Tahiti" Citrus aurantifolia, taking into account the following aspects: development and productivility of the trees quality and growth of fruits, demands of nutriments, and resistance or susceptibility to pest and disease; comparing its behavior with the commercial rootstocks rough, lemon C. jambhiri and mandarina, "Cleopatra" C. reticulata. En el C.N.l. Palmira del Instituto Colombiano Agropecuario (lCA), se evaluó la influencia del "Tabog" Swinglea glutinosa como porta-injerto para la lima "Tahiti " Citrus aurantifolia, teniendo en cuenta los siguientes aspectos: desarrollo y productividad del árbol, calidad y desarrollo de los frutos, exigencias de nutrimentos, resistencia o susceptibilidad a plagas y enfermedades; comparando su comportamiento con los porta-injertos comerciales limón "Rugoso" C. jambhiri y la mandarina "Cleopatra " C. reticulata.
Effect of Grafting Height on Success and Subsequent Growth of Acid Lime (Citrus aurantifolia Swingle) Saplings  [PDF]
B Chalise,KP Paudyal,SP Srivastava
Nepal Journal of Science and Technology , 2013, DOI: 10.3126/njst.v14i2.10412
Abstract: A field experiment was conducted at National Citrus Research Program (NCRP), Paripatle, Dhankuta to determine the best grafting height for the highest success of grafting and the maximum growth of saplings during 1st January to 30th December, 2010. Scions were collected from the mother plant ‘NCRP-49’ grown under screen house and grafted onto one-year-old trifoliate orange seedling rootstocks by shoot-tip method at 4 cm, 8 cm, 12 cm, 16 cm and 20 cm height from the collar region as the treatment. The grafts were planted inside the closed tunnel made from bamboo splits, jute and plastic sheet at 10×8 cm spacing in 64×100 cm experimental plots laid out in randomized complete block design (RCBD) with four replications containing 80 grafts per plot. Treatments were allotted on the experimental plots randomly. The success of grafting was not affected by the height of grafting, however, growth of sapling was found significantly affected by the height of grafting. Observation taken on saplings after one year of grafting revealed that the maximum scion height (42.13 cm), the highest number of leaves per sapling (47.50), the highest growth of scion diameter (55.61%), maximum length of primary branches (31.19 cm), maximum number of secondary branches per sapling ((3.24), the highest length of secondary branches (11.59 cm), the highest canopy volume (15440 cm 3 ) and the highest graft spread (24.35 cm) were found on the sapling grafted at 16 cm height of the trifoliate orange rootstock. Hence, from the study it is concluded that the most suitable height of grafting acid lime on trifoliate orange rootstock was 16 cm. DOI: http://dx.doi.org/10.3126/njst.v14i2.10412 ? Nepal Journal of Science and Technology Vol. 14, No. 2 (2013) 25-32
Variation of Physiochemical Components of Acid Lime (Citrus aurantifolia Swingle) Fruits at Different Sides of the Tree in Nepal  [PDF]
Ram Lal Shrestha, Durga Datta Dhakal, Durga Mani Gautum, Krishna Prasad Paudyal, Sangita Shrestha
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.312206
Abstract: Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of juice, TSS (Total soluble solids), TA (Titrable acid) and vitamin C are the determining factors of quality of acid lime fruits which may vary according to bearing sides of the trees. The main objective of this study is, to determine the variation of fruit quality at different sides of the tree. Total of 15 bearing trees were selected randomly (5 trees per site) from three different agro ecological domain representing terai (<600 m asl), mid hills (600 to 1200 m asl) and high hill areas (>1200 m asl) and samples were collected from the selected trees. Randomly ten fruits (from east, west, centre, north and south sides) were collected from each tree and analyzed for amount of vitamin C, TSS, TA and juice. Highest ascorbic acid 79.6 mg and 69.9 mg was observed in south side fruits whereas lowest 62.8 mg and 55.1 mg was observed in centre fruits in the high and mid hills zone respectively, but in terai, highest ascorbic acid 58.7 mg was observed in north side and lowest 41.8 mg was observed in centre. Highest amount of juice 43.9% was observed in south side fruits and lowest 36.6% in centre fruits, but amount of TSS 8.2% and TA 7.2% was observed in south side fruits and lowest 7.3% TSS and 7.0% TA was observed in centre fruits in high hills. In terai highest TSS 8.3% and TA 7.4% was observed in north side fruits and lowest TSS 7.3% and TA 6.7% was observed in centre fruits. Variation of TSS, TA percent and ascorbic acids was observed according to the agro ecological zone.
Propagación vegetativa de la lima ácida Tahiti Citrus aurantifolia (Christm) swingle, por medio de enraizamiento de estacas Vegetative spreading of sour lime Tahiti Citrus aurantifolia (Christm) swingle per rooting sticks  [cached]
Palma M. Ramíro,Baena García Diosdado,Escobar T. William
Acta Agronómica , 1992,
Abstract: En el ensayo definitivo adelantado en el Instituto Colombiano Agropecuario - Palmira, se evaluaron concentraciones de 150 y 250 ppm de ácido indolbutírico, tiempos de inmersión de 30 y 45 min., estacas con y sin disminución del área foliar, dos sustratos (cascarilla de arroz y una mezcla de suelo más arena), así como también la supresión de las tradicionales tapas de lienzo a las cámaras de propagación del sustrato suelo más arena. El porcentaje de formación de callo por el tipo de estímulo utilizado, fue similar en los dos sustratos. Los mejores resultados se obtuvieron con el tipo de estaca con hojas. La respuesta a las diferentes concentraciones de AIB fue relativamente similar. El tiempo de inmersión afectó en forma significativa el número de raíces, con promedio de seis raíces por estaca cuando se sometió a 45 mino de inmersión. At Instituto Colombiano Agropecuario - Palmira was made the present study. In the final essay was evaluated concentrations of 150 and 250 ppm of indolbutiric acid, inmersion times of 30 and 45 min., sticks with and without decreasing of leaf area, two substratum (rice's hust and a mix of soil plus sand), so also non- put covers of tinen cloth over propagation chambers of substratum soil plus sand. The percentage of caIlous formation by the kind of stimulus used, it was similar in both substratums. The best results were found in kind of stick with leaf. The answer of concentrations were similar. Time of inmersion affected in significant way the number of roots, getting six roots in average, when it's used 45 min. of inmersion.
Genetic Diversity Assessment of Acid Lime (Citrus aurantifolia Swingle) Landraces in Nepal, Using SSR Markers  [PDF]
Ram Lal Shrestha, Durga Datta Dhakal, Durga Mani Gautum, Krishna Prasad Paudyal, Sangita Shrestha
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.312204
Abstract: Acid lime (Citrus aurantifolia) is an important commercial fruits crop, cultivated in terai to high hills of Nepal. High variation of acid lime fruits are observed in existing landraces due to crossing within the other citrus species. Determination of genetic variation is important to the plant breeders for development of high yielding variety and hybrids. Therefore, an attempt has been made to study the genetic diversity of 62 acid lime landraces, collected from different altitudinal range in the eastern part of Nepal, using SSR markers. Twelve Simple Sequence Repeat (SSR) primer pairs were used to assess the genetic diversity of acid lime. The average genetic similarity level among the 62 accessions was 0.77, ranging from 0.54 to 1.0 and separated five major cluster groups. Total of 33 alleles were detected by eleven primer pairs and size of alleles ranged from 50 to 225. Average polymorphic information content (PIC) value was 0.50, whereas highest 0.75 and lowest 0.18 was observed in CAT01 and GT03 loci respectively. The results of the study clearly indicated that, SSR markers are highly polymorphic and more informative for the assessment of genetic diversity of acid lime landraces.
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