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Antigenic enzymes of Schistosoma mansoni: possible use for immunodiagnosis
M. Cesari, Italo;H. Pujol, Flor;Rodríguez, Margarita;Alarcón de Noya, Belkisyolé;
Memórias do Instituto Oswaldo Cruz , 1987, DOI: 10.1590/S0074-02761987000800030
Abstract: different enzymes of schistosoma mansoni are recognized by igg antibodies present in the sera of infected human patients. the antigenicity of these enzymes suggests their possible use in immunodiagnostic assays that would take advantage of their activities.
Isolation and characterization of partially purified leptospiral antigens
Koury, M.C.;Rangel, H.A.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1991, DOI: 10.1590/S0036-46651991000500009
Abstract: the methanol extract of leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. the antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. however the antigenicity was lost when the antigen was treated with periodic acid. chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. the complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.
Efecto antiviral de tinturas de Hypericum spp. cultivadas en Cuba
Castellanos Puerto,Edelis; Pérez De Alejo,José Luis; Machin Lugones,Maribel;
Revista Cubana de Plantas Medicinales , 2002,
Abstract: a study in vitro was made to demonstrate the antiretroviral activity of 2 hypericum species obtained in cuba (fasciculatum and styphelioids). hepatitis b virus surface antigen (hbsag) was used and mixed with both species at concentrations of 1,5,10,20and 30mg/ml and then incubated at 24h, 48h, 72h and 7d, 14d and 21d. then the reduction of antigenicity in vitro was evaluated by a sandwich-type immunoenzymagtic assay elisa that quantifies hbsag. it was observed that antigenicity decreased at 24 h for all the tested concentrations; however, from 72h on, a significant reduction of antigenicity occurred at ag concentrations below the limit values recorded for elisa tests performed. this study represents a possibility to be used for hbsag and in hiv treatment.
Infective viruses produced from full-length complementary DNA of swine vesicular disease viruses HK/70 strain
Haixue Zheng,Xiangtao Liu,Youjun Shang,Jinyan Wu,Xingwen Bai,Ye Jin,Shiqi Sun,Huichen Guo,Hong Tian,Xia Feng,Shuanghui Yin,Jianhong Guo,Guozheng Cong,Zaixin Liu,Huiyun Chang,Junwu Ma,Qingge Xie
Chinese Science Bulletin , 2006, DOI: 10.1007/s11434-006-2095-z
Abstract: The full-length cDNA clone of swine vesicular disease virus HK/70 strain named pSVOK12 was constructed in order to study the antigenicity, replication, maturation and pathogenicity of swine vesicular disease virus. In vitro transcription RNA from pSVOK12 transfected IBRS-2 cells and the recovered virus RNA were isolated and sequenced, then indirect hemagglutination test, indirect immunofluorescence assays, eleectron microscope test, 50% tissue culture infecting dose (TCID50) assays and mouse virulence studies were performed to study the antigenicity and virulence of the recovered virus. The result showed that the infectious clones we obtained and the virus derived from pSVOK12 had the same biological properties as the parental strain HK/70. The full-length infectious cDNA clone, pSVOK12, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of SVDV.
Infectious foot- and-mouth disease virus derived from a cloned full-length cDNA of OH/CHA/99
Guangqing Liu,Zaixin Liu,Qingge Xie,Yingli Chen,Huifang Bao,Xiangtao Liu
Chinese Science Bulletin , 2004, DOI: 10.1360/03wc0567
Abstract: The China foot- and-mouth virus (FMDV) isolate OH/CHA/99 was isolated from swine, which was unable to infect bovine thyroid cells in vitro or to cause typical disease in bovines following intradermal inoculation in the tongue. To enhance antigenicity, replication, maturation and pathogenicity studies of OH/CHA/99, an infectious fulllength cDNA clone, designated pBlFMDV, was prepared. The in vitro and in vivo biological properties of the virus derived from pBlFMDV were studied by analyzing antigenicity, plaque morphology and virulence in pigs. The results showed that the virus derived from pBlFMDV had the same biological properties as the parent strain OH/CHA/99; the fulllength infectious cDNA clone, pBlFMDV, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of FMDV.
Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA of OH/CHA/99
Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA of OH/CHA/99

Guangqing Liu,Zaixin Liu,Qingge Xie,Yingli Chen,Huifang Bao,Xiangtao Liu,
LIUGuangqing
,LIUZaixin,XIEQingge,CHENYingli,BAOHuifang,LIUXiangtao

科学通报(英文版) , 2004,
Abstract: The China foot-and-mouth virus (FMDV) iso-late OH/CHA/99 was isolated from swine, which was unable to infect bovine thyroid cells in vitro or to cause typical dis-ease in bovines following intradermal inoculation in the tongue. To enhance antigenicity, replication, maturation and pathogenicity studies of OH/CHA/99, an infectious full- length cDNA clone, designated pBlFMDV, was prepared. The in vitro and in vivo biological properties of the virus derived from pBlFMDV were studied by analyzing antigenicity, plaque morphology and virulence in pigs. The results showed that the virus derived from pBlFMDV had the same biologi-cal properties as the parent strain OH/CHA/99; the full- length infectious cDNA clone, pBlFMDV, will be very useful in studies of the antigenicity, virulence, pathogenesis, matu-ration and replication of FMDV.
Genetic Diversity of Staphylocoagulase Genes (coa): Insight into the Evolution of Variable Chromosomal Virulence Factors in Staphylococcus aureus
Shinya Watanabe,Teruyo Ito,Takashi Sasaki,Shanshuang Li,Ikuo Uchiyama,Kozue Kishii,Ken Kikuchi,Robert Leo Skov,Keiichi Hiramatsu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0005714
Abstract: The production of staphylocoagulase (SC) causing the plasma coagulation is one of the important characteristics of Staphylococcus aureus. Although SCs have been classified into 10 serotypes based on the differences in the antigenicity, genetic bases for their diversities and relatedness to chromosome types are poorly understood.
The Concentration of Carbon Source in the Medium Affects the Quality of Virus-Like Particles of Human Papillomavirus Type 16 Produced in Saccharomyces cerevisiae  [PDF]
Hyoung Jin Kim, Yingji Jin, Hong-Jin Kim
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094467
Abstract: There is accumulating evidence that virus-like particles (VLPs) recombinantly produced in Saccharomyces cerevisiae (S. cerevisiae) are characterized by low structural stability, and that this is associated with reduced antigenicity and immunogenicity. However, little attention has been devoted to methods of improving the quality of the VLPs. Here, we investigated the effect of carbon source concentration in the medium on the antigenicity and immunogenicity of human papillomavirus (HPV) type 16 L1 VLPs expressed in S. cerevisiae from the galactose promoter. Media containing 2, 4, 6, and 8% carbon source, composed of both glucose and galactose in equal proportion, were used. VLP antigenicity was enhanced in cultures grown on media with 6 or 8% carbon source, compared to those from cultures with less than 6% carbon source. Moreover, the VLPs obtained from these cultures induced higher anti-HPV16 L1 IgG titers and neutralizing antibody titers in immunized mice than those purified from cultures with less than 6% carbon source. Our results indicate that the concentration of the carbon source in the medium plays a crucial role in determining the antigenicity and immunogenicity of HPV type16 L1 VLPs.
Expression and characterization of hepatitis C virus core protein fused to hepatitis B virus core antigen
Li Yang,Chunlin Wang,Yuan Wang,Guangdi Li
Science China Life Sciences , 1999, DOI: 10.1007/BF03183605
Abstract: Recombinant plasmids were constructed by fusing the gene fragments encoding the full-length (1-191aa) and the truncated (1-40aa and l-69aa) HCV core proteins (HCc) respectively to the core gene of HBV at the position of amino acid 144 and expressed inE. coli. The products were analyzed by ELISA, Western blotting as well as the immunization of the mice. The results showed that those fusion proteins (B144C191, B144C69, B144C40) possessed the dual antigenicity and immunogenicity of both hepatitis B virus core antigen (HBcAg) and hepatitis C virus core protein (HCc). Analysis by electron microscopy and CsCI density gradient ultra-centrifugation revealed that similar to the HBcAg itself, all fusion proteins were able to form particles. Comparison of the antigenicity and immunogenicity of those fusion proteins showed that the length of HCc gene fused to HBcAg had no much effect on the antigenicity and immunogenicity of HBcAg, however, B144C69 and B144C40 induced higher titres antibodies against HCc than B144C191. Using those fusion proteins, ELISA for screening of antibodies against both HBV and HCV in human sera was also established.
Expression and characterization of hepatitis C virus core protein fused to hepatitis B virus core antigen
YANG Li,WANG Chunlin,WANG Yuan,LI Guangdi,
杨莉
,王春林,汪垣,李光地

中国科学C辑(英文版) , 1999,
Abstract: Recombinant plasmids were constructed by fusing the gene fragments encoding the full-length (l-191aa) and the truncated (l-40aa and l-69aa) HCV core proteins (HCc) respectively to the core gene of HBV at the position of amino acid 144 and expressed in E. coli. The products were analyzed by ELISA, Western blotting as well as the immunization of the mice. The results showed that those fusion proteins (B144C191, B144C69, B144C40) possessed the dual antigenicity and immunogenicity of both hepatitis B virus core antigen (HBcAg) and hepatitis C virus core protein (HCc). Analysis by electron microscopy and CsCl density gradient ultra-centrifugation revealed that similar to the HBcAg itself, all fusion proteins were able to form particles. Comparison of the antigenicity and immunogenicity of those fusion proteins showed that the length of HCc gene fused to HBcAg had no much effect on the antigenicity and immunogenicity of HBcAg, however, B144C69 and B144C40 induced higher titres antibodies against HCc than B144C191. Using those fusion proteins, ELISA for screening of antibodies against both HBV and HCV in human sera was also established.
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