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Neonatal sepsis due to Weeksella virosa  [cached]
Anuradha Mokkapati
International Journal of Research in Medical Sciences , 2013, DOI: 10.5455/2320-6012.ijrms20130829
Abstract: Weeksella virosa was previously included in group II f of CDC. We here present the Microbiological characteristics of the isolate from a case of neonatal sepsis at our center. The organism is a non-fermenter growing only on blood agar and not on Mac Conkey agar, oxidase and catalase positive, and negative for several other bio-chemical tests, except for indole with Ehrlich s reagent. The isolate in the present case study was sensitive to aminoglycosides and β- lactams, and resistant to quinolones and carbapenems. [Int J Res Med Sci 2013; 1(3.000): 294-295]
Trichomes and hydrocarbons associated with the tomato plant antixenosis to the leafminer
Fernandes, Maria Elisa S.;Fernandes, Flávio L.;Silva, Derly J.H.;Pican?o, Marcelo C.;Jhamc, Gulab N.;Carneiro, Pedro C.;Queiroz, Renan B.;
Anais da Academia Brasileira de Ciências , 2012, DOI: 10.1590/S0001-37652012000100021
Abstract: the objective of this work was to study the density of trichomes and hydrocarbons associated with the resistance by antixenosis of 42 subsamples of tomato plants from the horticultural germplasm bank of the universidade federal de vi?osa (hgb-ufv) to liriomyza trifolii. these subsamples were studied in addition to 'santa clara' cultivar, which was used as a standard of susceptibility to leafminers. the evaluated characteristics were the number of mined leaflets per plant, mines per plant, trichome density and chemical compounds in the leaves. differences were detected among the subsamples in the variables studied. we identified 20 peaks in the chromatograms of leaf hexane extract the subsamples tested. the subsamples hgbs - 216, 813, 985, 987, 991, 992, 993, 1532, 1989, 1991, 2048, 2055, 2064, 2068, 2073, 2075, 2089, 2096 and 2097 were selected as sources of resistance to l. trifolii. the resistance mechanism associated to these subsamples was antixenosis. in addition, the low density of trichomes and the chemical compounds in the subsamples can be possible causes of pest resistance.
Hairy root culture of Lactuca virosa L.
Anna Stojakowska,Janusz Malarz,Wanda Kisiel,Stanis?aw Kohlmünzer
Acta Societatis Botanicorum Poloniae , 1995, DOI: 10.5586/asbp.1995.006
Abstract: A hairy root culture of Lactuca virosa L. (Asteraceae) was initiated by infection of the leaf explants of the aseptic plants with Agrobacterium rhizogenes - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant.
Antixenosis to the Peach-potato Aphid, Myzus persicae (Sulzer) in Potato Cultivars  [PDF]
A.U.R. Saljoqi,H.F. van Emden,He Yu-rong
Asian Journal of Plant Sciences , 2003,
Abstract: Field studies were conducted to determine the antixenotic resistance against the peach-potato aphid, Myzus persicae (Sulzer) in different potato cultivars i.e. Cardinal, Ultimus and Desiree. These cultivars were studied in the paired tests as Cardinal vs. Desiree and Cardinal vs. Desiree for antixenosis (systemic insecticide-based test). In Desiree vs. Ultimus, no significant difference was found for the preference of M. persicae, while in case of Cardinal vs. Desiree, there was a significant preference for Desiree. Cardinal clearly showed antixenosis to M. persicae.
Antixenosis of Brevicoryne brassicae on Different Genotypes of Cabbage (Brassica oleracea Var. Capitata)  [PDF]
Muhammad Younis Jatoi,Muhammad Aslam,Misbah-ul-Haq,Shakeel Ahmad
Journal of Biological Sciences , 2001,
Abstract: Antixenosis of Brevicoryne brassicae on different genotypes of cabbage (Brassica oleracea var. capitata) was studied.The Golden acre, C110, B25, 14YXMEK2, DEC1XB21,CF2XLI2-11, and CF1XDT46 showed statistically less number of aphids while CF1XCF2, CF2XE34_A1 and DEC1XRB4PE attracted more number of aphids and the aphid thus showed more preference on these genotypes as compared to the others. The native variety Golden acre from Pakistan although attracted the least number of aphids but the number was not statistically different from the others mentioned earlier. The aphid, Brevicoryne brassicae discriminated among different cabbage genotypes and environmental factors including temperature, humidity and rainfall influenced the number of aphids per plant.
Anti-diabetic properties of Securinega virosa (Euphorbiaceae) leaf extract
Y Tanko, MA Okasha, GM Magaji, M Yerima, AH Yaro, MIA Saleh, A Mohammed
African Journal of Biotechnology , 2008,
Abstract: This study was undertaken to evaluate the hypoglycemic effect of methanol extract of securinega virosa leaves on blood levels of streptozocin-induced diabetes rats. Three doses of the extract (100, 300 and 600 mg/kg) were administered intraperitoneally. After 2 h of extract administration there was no significant change in the blood glucose levels in all the three doses of the extract. Also after 4, 8 and 24 h of extract administration there was a significant (p < 0.05 - 0.001) decrease in the blood glucose levels in all the three doses of the extract. The preliminary phytochemical screening revealed the presence of reducing sugars, cardiac glycosides, resin, tannins, saponins, glycosides, flavonoids, glycerin carbohydrate, anthraquine and steroids. The median lethal dose (LD50) in rats was calculated to be 1264.9 mg/kg body weight.
Sepsis due to Weeksella virosa in wounds made by animal bite. A case report. Sepsis por weeksella virosa en herida por mordedura animal. Reporte de un caso.  [cached]
Elio Jesús Nú?ez Tamayo,Susana Chamero Melgarejo,Nancy Ramírez Martínez
MediSur , 2003,
Abstract: This paper contains a case report of acute sepsis due to Weeksella virosa, a Gram negative micro-organism non fermentative of positive oxidase carbohidrates in a healthy patient aged 31 who was assisted at the service of microbiology of the ¨Dr. Gustavo Aldereguía Lima¨ Hospital in March 2003. The clinical symptoms were fever, pain, limited movements, erythema and sensation of warm. A diagnosis of reticular lymphangitis was done and Weeksella virosa was isolated by a conventional diagnosis. Tests for antimicrobial susceptibility showed sensitivity to tetracycline, aztreonam, ceftriazone and resistance to imipenem. Treatment was applied with tetracycline 1 gr. Daily for 7 days. The clinical picture improved with total regression of symptom. Se reporta un caso de sepsis aguda por Weeksella virosa, un microorganismo gramnegativo, no fermentador de carbohidratos oxidasa positiva, en una paciente sana, de 31 a os, atendida en el servicio de Microbiología del Hospital Universitario ¨Dr. Gustavo Aldereguía Lima¨, en marzo del 2003. Los síntomas clínicos fueron: fiebre, dolor, limitación de movimientos, eritema y calor, a la cual se le diagnosticó linfangitis reticular. Se aisló Weeksella virosa por diagnóstico convencional. En las pruebas de susceptibilidad antimicrobiana resultó sensible a tetraciclina, aztreonam, ceftriazona y resistente a imipenem. Recibió tratamiento con tetraciclina 1 gr diario por siete días. El cuadro clínico mejoró con regresión total de los síntomas.
Preliminary antidiarrhoeal activity of methanolic extracts of Securinega virosa (Euphorbiaceae)
MG Magaji, AH Yaro, A Mohammed, AU Zezi, Y Tanko, TY Bala
African Journal of Biotechnology , 2007,
Abstract: Securinega virosa is used as remedy for diarrhoea in tropical Africa, but has not been investigated for its antidiarrhoeal activity. This study was therefore aimed at investigating the methanolic extracts of the leaves, stem bark and root bark for antidirrhoeal activity, using castor oil-induced diarrhoeal model in mice. The effects of these extracts on perfused isolated rabbit jejunum were also evaluated. The methanolic leaves extract (8 x 10-5 – 1.6 x 10-3 mgml-1) produced a dose-dependent relaxation of the rabbit jejunum, while the methanolic stem bark and root bark extracts (2 x 10-5 – 3.2 x 10-3 mgml-1) produced contraction of the tissue. The methanolic root bark extract produced a dose-dependent protection against the castor oil- induced diarrhoea with the highest protection (100%), obtained at 100 mgkg-1 comparable to that of loperamide (5 mgkg-1), the standard agent. The leaves extract also protected the mice but was not dose-dependent. The highest protection (60%) was obtained at the lowest dose (50 mgkg-1). The stem bark extract did not protect the animal against diarrhoea. The preliminary phytochemical analysis revealed that the three extracts contained similar phytochemical constituents which include alkaloids, tannins, saponins, flavonoids and cardiac glycosides. However, only the leaves extract contained anthraquinone glycosides. The acute toxicity test revealed the median lethal dose (LD50) values for the leaves, stem bark and root bark extracts to be 1265, 288.5 and 774.6 mgkg-1 respectively. This suggests that the stem bark extract is relatively the most toxic. These results obtained revealed that the leaves and root bark extracts possess pharmacological activity against diarrhoea and may possibly explain the use of the plant in traditional medicine.
Effect of methyl jasmonate on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.
Janusz Malarz,Wanda Kisiel
Acta Societatis Botanicorum Poloniae , 1999, DOI: 10.5586/asbp.1999.017
Abstract: Hairy roots of Lactuca virosa L. transformed with Agrobacterium rhizogenes and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control.
Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.
Janusz Malarz,Wanda Kisiel
Acta Societatis Botanicorum Poloniae , 2000, DOI: 10.5586/asbp.2000.014
Abstract: Direct addition of pectinase as an elicitor to the hairy root culture of Lactuca virosa L. transformed with Agrobacterium rhizogenes stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment.
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