oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
Actividad antibacteriana de Pelargonium peltatum (L.) L'Hér. sobre Streptococcus mutans, Streptococcus sanguis y Streptococcus mitis frente a clorhexidina Antibacterial activity of Pelargonium peltatum (L.) L'Her. against Streptococcus mutans, Streptococcus sanguis and Streptococcus mitis versus chlorhexidine
Juana del Carmen Guerrero Hurtado,Zoila Mercedes Ortiz Rubio,Luis Fernando Peralta Berrospi,Fredy Romel Pérez Azahuanche
Revista Cubana de Plantas Medicinales , 2013,
Abstract: Introducción: Pelargonium peltatum (L.) L'Hér (geranio hiedra) es ampliamente utilizado en medicina natural para el tratamiento de enfermedades bucales, pero se desconocen aún sus propiedades farmacológicas, actividad antibacteriana y la composición de sus fitoconstituyentes. Objetivo: realizar un estudio comparativo de la actividad antibacteriana in vitro del extracto acuoso obtenido de las hojas de Pelargonium peltatum (L.) L'Hér (geranio hiedra) sobre Streptococcus mutans, Streptococcus sanguis y Streptococcus mitis, frente a la clorhexidina. Métodos: para el ensayo antibacteriano se empleó el método de difusión en agar. Se trabajó con 18 muestras de microorganismos de cada especie mencionada, aisladas de los pacientes de una clínica dental. Posteriormente, se prepararon 6 concentraciones diferentes del extracto acuoso, para comparar la actividad antibacteriana frente al colutorio de clorhexidina. El análisis fitoquímico preliminar se realizó mediante el ensayo a la gota. Los datos obtenidos se sometieron a análisis estadísticos como estimadores de media y dispersión, análisis de varianza unifactorial y prueba de Tukey. Resultados: la más alta actividad antibacteriana se obtuvo en la concentración de 400 mg/mL y la más baja en la de 25 mg/mL del extracto acuoso, sobre las tres especies de Streptococcus, en comparación con la clorhexidina; con efecto similar a la concentración de 200 mg/mL. El ensayo fitoquímico preliminar indicó la presencia de flavonoides, taninos, esteroides, antocianinas y saponinas. Conclusiones: el extracto acuoso de Pelargonium peltatum tiene actividad antibacteriana sobre Streptococcus mutans, Streptococcus mitis y Streptococcus sanguis. Introduction: Pelargonium peltatum (L.) L'Her (ivy geranium) is widely used in natural medicine for the treatment of oral disease, but its pharmacological properties, antibacterial activity and phytoconstituent composition are still unknown. Objective: carry out a comparative study of the in vitro antibacterial activity of the aqueous extract obtained from leaves of Pelargonium peltatum (L.) L'Her (ivy geranium) against Streptococcus mutans, Streptococcus sanguis and Streptococcus mitis versus chlorhexidine. Methods: the agar diffusion method was used for the antibacterial assay. A study was conducted of 18 samples of microorganisms from the above-mentioned species, isolated from patients cared for at a dental clinic. Six different concentrations were prepared of the aqueous extract to compare antibacterial activity versus the chlorhexidine gargle. Preliminary phytochemical analysis was conduc
Comparison of inhibitory dilutions of a thymol-based mouthwash (Orion ) with chlorhexidine on Streptococcus mutans and Streptococcus sanguis
Mohammadmahdi Yaghooti Khorasani,Sepideh Assar,Omid Rezahosseini,Shokrollah Assar
Journal of Isfahan Dental School , 2011,
Abstract: Introduction: Applying the suitable mouthwash can kill bacteria or reduce the bacterial growth; in the same context, diluting mouthwashes can decrease their probable side effects. In this study, the maximum inhibitory concentrations of two common mouthwashes were evaluated.Materials and Methods: In this in vitro study, agar dilution and disk diffusion methods were used. Sixty-nine bacterial plates were cultured to evaluate the effects of thymol and chlorhexidine mouthwashes on Streptococcus mutans and Streptococcus sanguis. The results of agar dilution method were recorded as "growth' and "no growth", and the results of disk diffusion were recorded as means of growth inhibitory halos. Data was analyzed by Kruskal-Wallis test using SPSS software. (α = 0.05).Results: Growth inhibitory halos for different concentrations of the two mouthwashes decreased with an increase in dilution rate. In agar dilution method, dilutions less than 80% of the initial concentration for thymol-based mouthwash and less than 70% for chlorhexidine could not inhibit bacterial growth. Growth inhibition halos with 100% thymol-based mouthwash (Orion ) were 10.15 ± 0.1 mm for Steptococcus mutans and 9.2 7 ± 0.16 mm for Streptococcus sanguis. Mean rank of the two mouthwashes was statistically significant for the two bacterial strains under study (p value < 0.001).Conclusion: Both mouthwashes were effective on the bacteria under study; however, this efficacy was higher for Streptococcus sanguis. Chlorhexidine mouthwash was more potent to inhibit bacterial growth than thymol-based mouthwash if diluted. Key words: Mouthwash, Chlorhexidine, Streptococcus mutans, Streptococcus sanguis, Thymol.
Atividade antimicrobiana in vitro do extrato de Anacardium occidentale L. sobre espécies de Streptococcus
Melo, Arquimedes F. Monteiro de;Santos, Eliane J. Vasconcelos;Souza, Luiza F. Cordeiro de;Carvalho, Alessandra de Albuquerque T.;Pereira, Maria do Socorro V.;Higino, Jane S.;
Revista Brasileira de Farmacognosia , 2006, DOI: 10.1590/S0102-695X2006000200012
Abstract: the antimicrobial activity of the extract of the stem bark of anacardium occidentale l., was evaluated in three cultures of bacteria of the dental plate. the antimicrobial activity was assayed in petri dishes using the diffusion method for determination of the minimal inhibitory concentration (mic) and minimal inhibitory concentration of adherence (mica). aureolar inhibition (mic) was observed with concentrations of 12.5 mg/ml in s. mutans and 6.25 mg/ml in s. mitis and s. sanguis. the results suggest that the extract has effect in the mica in concentrations of 0.31 mg/ml in s. mutans and s. mitis and of 0.15 mg/ml in s. sanguis and therapeuticly can be used in the dentistry as an antibacterial agent.
Assessing the Effect of Pineapple Extract Alone and in Combination With Vancomycin on Streptococcus sanguis
Zahra Emami,Hengameh Khosropanah,Abdollah Bazargani,Hooman Ebrahimi
Jundishapur Journal of Natural Pharmaceutical Products , 2012,
Abstract: Background: Periodontitis is a major problem that affects a large number of patients in the society. Various treatment alternatives have been proposed to control this pathologic condition. Streptococcus sanguis is one of the countless pathogens involved in periodontitis.Objectives: The current study aimed to assess the efficacy of pineapple extract per se and in synergy with vancomycin on the growth activity of S. Sanguis.Materials and Methods: An experimental study was designed to determine the minimum inhibitory concentration (MIC) of Pineapple extract per se and in conjunction with vancomycin. The study was carried out in three stages. Serial concentrations of the aqueous pineapple extract, vancomycin, and pineapple plus vancomycin were prepared by broth microdilution technique respectively and were exposed to the standard laboratory strain of S. sanguis (10556 ATCC). The lowest concentration of the pineapple extract and the mixed pineapple/vancomycin solution which inhibited bacterial growth was recorded as the MIC.Results: The minimum inhibitory concentration of vancomycin was determined 1 μg/ ml. The pineapple extract failed to show any inhibitory effects per se, however, once added to vancomycin, it reduced the MIC to 0.5μg/ml.Conclusions: Prescription of pineapple extract along with antibiotics increases the antibacterial effects of the drug, therefore reduces the minimum inhibitory concentration of the antibiotic.
Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans
Vasconcelos, Laurylene César de Souza;Sampaio, Fábio Correia;Sampaio, Maria Carmélia Correia;Pereira, Maria do Socorro Vieira;Higino, Jane Sheila;Peixoto, Maria Helena Pereira;
Brazilian Dental Journal , 2006, DOI: 10.1590/S0103-64402006000300009
Abstract: the purpose of this study was to investigate the antimicrobial effect of a punica granatum linn (pomegranate) phytotherapeutic gel and miconazole (daktarin? oral gel) against three standard streptococci strains (mutans atcc 25175, sanguis atcc 10577 and mitis atcc 9811), s. mutans clinically isolated and candida albicans either alone or in association. the effect of minimum inhibitory concentrations of the gels on the adherence of these microorganisms to glass was assessed in the presence of 5% sucrose, using increasing and doubled concentrations of the diluted solution of the gels ranging from 1:1 to 1:1024. the minimum inhibitory concentrations of adherence of punica granatum l. gel against the test organisms were: 1:16 for s. mutans (atcc), s. mutans (ci) and s. sanguis; 1:128 for s. mitis and 1:64 for c. albicans. the minimum inhibitory concentrations of adherence of miconazole against the same organisms were: 1:512, 1:64, 1:4, 1:128 and 1:16, respectively. in experiments with three and four associated microorganisms, the punica granatum l. gel had greater efficiency in inhibiting microbial adherence than the miconazole. the results of this study suggest that this phytotherapeutic agent might be used in the control of adherence of different microorganisms in the oral cavity.
Efeito antibacteriano e antiaderente in vitro do extrato da Punica granatum Linn. sobre microrganismos do biofilme dental
Pereira, Jozinete V.;Pereira, Maria S. Vieira;Sampaio, Fábio C.;Sampaio, Maria C. Correia;Alves, Pollianna M.;Araújo, Cristina R. Ferreira de;Higino, Jane S.;
Revista Brasileira de Farmacognosia , 2006, DOI: 10.1590/S0102-695X2006000100016
Abstract: in this study was evaluated the in vitro antibacterial activity and the inhibition of glucan sintesis of the fruit bark of punica granatum linn extract upon the following dental biofilm bacteria: streptococcus mitis, streptococcus mutans, streptococcus sanguis, streptococcus sobrinus and lactobacillus casei. the tests were carried out by inundation tecniques in petri dishes to determine the minimum inhibitory concentration (mic) and inclined tubes techniques to determine the minimum inhibitory concentration of adherence to glass (mica) at the presence of 5% sucrose. tests with gluconate of chlorexidine (0.12%) were performed as controls. mics of the p granatum extract dilutions (mg/ml) against s. mitis, s. mutans, s. sanguis, s. sobrinus e l. casei were 1:32, 1:8, 1:8, 1:512, and 1:128 respectively. for micas the p. granatum showed better results than chlorexidine, except for s. mutans with both substances showing similar results of 1:256. it can be concluded that the extract of the p. granatum has the potential to inhibit oral bacterial growth and glucan sintesis. the extract of the punica granatum might be used as an effective antibacterial alternative agent against oral biofilm bacteria.
Antimicrobial Activity of Traditional Chinese Medicines on Common Oral Bacteria  [PDF]
Michelle K. Z. Yuen, Ricky W. K. Wong, Urban H?gg, Lakshman Samaranayake
Chinese Medicine (CM) , 2011, DOI: 10.4236/cm.2011.22007
Abstract: Objective: To evaluate twenty Traditional Chinese Medicines (TCM) against four oral bacteria. Methods: Twenty TCM were tested for sensitivity against Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis. Aliquots of suspension of each bacterial species were inoculated on a horse blood agar (HBA) plate, 6 mm diameter paper disks was soaked in different drug suspensions were placed concentrically on a HBA plate. Disks soaked in 0.2% w/v chlorhexidine were used as positive controls. These HBA plates were incubated for 48 hours anaerobically and the diameters of growth inhibition of three different areas were measured using a calibrated computer software and the mean diameter obtained for each bacteria. Broth microdilution assay was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The experiment was repeated on three separate occasions. Results: The TCMs that consistently against Porphyromonas gingivalis, included Folium artemisiae argyi, Fructus crataegi, Rhizoma dryopteris crassirhizomae, Flos magnoliae, Rhizoma polygoni cuspidati, Radix scrophulariae ningpoensis, Galla chinensis, Radix scutellariae baicalensis and Rhizoma coptidis; against Streptococcus mutans included Fructus crataegi, Galla chinensis and Rhizoma copitidis; against Streptococcus mitis and Streptococcus sanguis included Galla chinensis and Rhizoma copitidis. Conclusion: Rhizoma copitidis and Galla chinensis had inhibitory effects on Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans and Porphyromonas gingivalis in vitro.
The Genome of Streptococcus mitis B6 - What Is a Commensal?  [PDF]
Dalia Denapaite,Reinhold Brückner,Michael Nuhn,Peter Reichmann,Bernhard Henrich,Patrick Maurer,Yvonne Sch?hle,Peter Selbmann,Wolfgang Zimmermann,Rolf Wambutt,Regine Hakenbeck
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009426
Abstract: Streptococcus mitis is the closest relative of the major human pathogen S. pneumoniae. The 2,15 Mb sequence of the Streptococcus mitis B6 chromosome, an unusually high-level beta-lactam resistant and multiple antibiotic resistant strain, has now been determined to encode 2100 genes. The accessory genome is estimated to represent over 40%, including 75 mostly novel transposases and IS, the prophage φB6 and another seven phage related regions. Tetracycline resistance mediated by Tn5801, and an unusual and large gene cluster containing three aminoglycoside resistance determinants have not been described in other Streptococcus spp. Comparative genomic analyses including hybridization experiments on a S. mitis B6 specific microarray reveal that individual S. mitis strains are almost as distantly related to the B6 strain as S. pneumoniae. Both species share a core of over 900 genes. Most proteins described as pneumococcal virulence factors are present in S. mitis B6, but the three choline binding proteins PcpA, PspA and PspC, and three gene clusters containing the hyaluronidase gene, ply and lytA, and the capsular genes are absent in S. mitis B6 and other S. mitis as well and confirm their importance for the pathogenetic potential of S. pneumoniae. Despite the close relatedness between the two species, the S. mitis B6 genome reveals a striking X-alignment when compared with S. pneumoniae.
Optimization of physical and nutritional parameters for hyaluronidase production by Streptococcus mitis  [cached]
Sahoo S,Panda P,Mishra S,Nayak A
Indian Journal of Pharmaceutical Sciences , 2008,
Abstract: The effect of some physical and nutritional parameters were studied for the optimum production of extracellular enzyme hyaluronidase employing Streptococcus mitis MTCCFNx012695 by submerged fermentation. The effects of initial pH, incubation temperature and time, inoculum level and age of inoculum were studied. The maximum enzymatic activity was obtained with an initial pH 5.8, incubation temperature 37°, incubation time for 48 h and inoculum level 6% with inoculum age 24 h. The effect of different carbon and nitrogen sources and antibiotics were studied. The results indicated that sucrose and ammonium chloride showed the highest enzymatic activity among various carbon and nitrogen sources. Antibiotic clarithromycin showed strong inhibitory effect on hyaluronidase production.
Efeito antimicrobiano in vitro do extrato de jabuticaba [Myrciaria cauliflora (Mart.)O.Berg.] sobre Streptococcus da cavidade oral
Carvalho, C.M.;Macedo-Costa, M.R.;Pereira, M.S.V.;Higino, J.S.;Carvalho, L.F.P.C.;Costa, L.J.;
Revista Brasileira de Plantas Medicinais , 2009, DOI: 10.1590/S1516-05722009000100013
Abstract: this study evaluated the action of ethanolic extract of myrciaria cauliflora (mart.)o.berg leaves on three streptococcus cultures from dental plaque. antimicrobial activity was assessed by agar-well diffusion method. the obtained data were satisfactory for all assayed species (streptococcus mutans atcc 25175, streptococcus sobrinus atcc 27609 and streptococcus sanguis atcc 10557) yielding inhibition halos when the pure extract and 1:2 and 1:4 dilutions were used. these results encourage new studies for the production of therapeutic compounds to be used in clinical dentistry.
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.