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SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND LINEZOLID IN TABLET DOSAGE FORM  [PDF]
Patel Satish A,Patel Jinalben V
International Research Journal of Pharmacy , 2013,
Abstract: The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Cefixime Trihydrate and Linezolid in bulk and tablet dosage form. The method is based on the simultaneous equations for analysis of both the drugs using 0.05 M Potassium phosphate buffer pH 7.2 as solvent. Cefixime Trihydrate has absorbance maxima at 287.20 nm and Linezolid has absorbance maxima at 250.60 nm in 0.05 M Potassium phosphate buffer pH 7.2. The linearity was obtained in the concentration range of 2-22 μg/ml and 2-18 μg/ml for Cefixime Trihydrate and Linezolid, respectively. The concentrations of the drugs were determined by using simultaneous equations at both the wavelengths. The mean recovery was 100.2 ± 0.56 and 101.23 ± 0.63 for Cefixime Trihydrate and Linezolid, respectively. The method was successfully applied to tablet dosage form. The suitability of this method for the quantitative determination of Cefixime Trihydrate and Linezolid was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Cefixime Trihydrate and Linezolid in combination. The results of analysis have been validated statistically and by recovery studies.
SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF CEFIXIME TRIHYDRATE AND OFLOXACIN IN TABLETS  [PDF]
Patel Satish A,Patel Paresh U,Patel Natavarlal J.
International Research Journal of Pharmacy , 2011,
Abstract: The present manuscript describe simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of cefixime trihydrate and ofloxacin in combined tablet dosage form. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Cefixime trihydrate and ofloxacin show an isoabsorptive point at 280.2 nm in methanol. The second wavelength used is 291.4 nm, which is the λ-max of cefixime trihydrate in methanol. The linearity was obtained in the concentration range of 2-14 μg/ml for both cefixime trihydrate and ofloxacin. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of cefixime trihydrate. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The results of analysis have been validated statistically and by recovery studies.
DEVELOPMENT AND VALIDATION OF SIMULTANEOUS EQUATION METHOD FOR THE ESTIMATION OF CEFIXIME AND OFLOXACIN IN PURE AND IN TABLET FORMULATION  [PDF]
Ramesh J,Arul Prakasam K.C,Suresh A,Venkatesh Kothapalli
International Research Journal of Pharmacy , 2011,
Abstract: This paper describes developed and validated UV spectrophotometry method for the simultaneous estimation of Ofloxacin and Cefixime in a combined dosage form by Simultaneous equation method. This method utilize methanol as solvent. In this method UV spectra of Ofloxacin and Cefixime were recorded, from the overlay spectra the wavelength selected for the simultaneous equation method were 235nm for Ofloxacin and 290nm for Cefexime, where the linearity ranges for both the drugs were 5-30 μg / ml. The correlation coefficient of Ofloxacin and Cefexime was found to be 0.9999 and 0.9998 respectively. The results of analysis of this method have been validated statistically and recovery studies confirmed the accuracy of the proposed method.
DEVELOPMENT AND VALIDATION OF DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME TRIHYDRATE AND AZITHROMYCIN DIHYDRATE IN COMBINED DOSAGE FORM  [PDF]
Vishal Shah* and Hasumati Raj
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: A novel, simple, accurate, sensitive, reproducible, economical spectroscopic method was developed and validated for the determination of Azithromycin dihydrate and Cefixime trihydrate in combined dosage form. Second order derivative spectroscopy method is adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 10-40 ppm for Cefixime trihydrate and 25-100 ppm of Azithromycin dihydrate. The method was validated for linearity, accuracy and precision as per ICH guidelines. The zero crossing point for Azithromycin dihydrate and Cefixime trihydrate was 326.4 nm and 226.8 nm, respectively in water. The LOD and LOQ value were found to be 0.54 and 1.64 ppm for Cefixime trihydrate and 0.77 and 2.34 ppm for Azithromycin dihydrate respectively. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form (ZIMNIC -AZ).
Simultaneous RPHPLC determination of nitazoxanide and ofloxacin in combined tablet dosage form  [cached]
Kalta R,Sharma R,Chaturvedi S
Indian Journal of Pharmaceutical Sciences , 2008,
Abstract: A simple, precise, accurate, rapid and reproducible reverse phase high performance liquid chromatographic procedure was developed for simultaneous determination of nitazoxanide and ofloxacin in tablet dosage form at a single wavelength. The mobile phase used was a combination of acetonitrile:0.25M potassium dihydrogen phosphate buffer (80:20) with 0.5%v/v of triethylamine and the pH was adjusted to 2.5 by adding orthophosphoric acid. The detection of the combined dosage form was carried out at 320 nm and flow rate was set to 1ml/min. Linearity was obtained in the concentration range of 5 to 25 mg/ml of nitazoxanide and ofloxacin with correlation coefficients of 0.9987 and 0.9995, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.
Study of degradation profile and development of stability indicating methods for cefixime trihydrate  [cached]
Gandhi S,Rajput S
Indian Journal of Pharmaceutical Sciences , 2009,
Abstract: The degradation behavior of cefixime trihydrate was investigated under different stress conditions of acidic hydrolysis, alkaline hydrolysis and oxidation using spectrophotometry. Stability indicating spectrophotometric methods were developed that could separate the drug from its degradation products formed under these stress conditions. The UV spectral characteristics of the drug and degraded products were quite different and zero and first order derivative ultraviolet spectrophotometric methods were used to study the extent of degradation. Cefixime trihydrate was found to degrade extensively under experimental conditions. The methods were validated by establishing the linearity, inter and intraday precision, accuracy, selectivity and specificity.
Validated high performance thin layer chromatography method for simultaneous estimation of ofloxacin and ornidazole in tablet dosage form
Gandhimathi M,Ravi T,Shukla Nilima
Indian Journal of Pharmaceutical Sciences , 2006,
Abstract: A simple, accurate and precise high performance thin layer chromatographic method has been developed for the estimation of ofloxacin and ornidazole simultaneously in tablet dosage form. The method employed silica gel 60GF254 precoated plates as stationary phase and a mixture of n-butanol: ethanol: ammonia (5:5:4 %v/v/v) as mobile phase. The plate was scanned and quantified at 295 nm using Camag TLC scanner. The method was validated for linearity, accuracy, precision, repeatability and specificity, proving its utility in estimation of ofloxacin and ornidazole in combined dosage form.
Development and validation of spectroscopic methods for simultaneous estimation and dissolution of ofloxacin and ornidazole in tablet dosage forms
Mashru, R.C;Saikumar, S.V;
Eclética Química , 2010, DOI: 10.1590/S0100-46702010000300012
Abstract: the aim of this work was to develop and validate simple, accurate and precise spectroscopic methods (multicomponent, dual wavelength and simultaneous equations) for the simultaneous estimation and dissolution testing of ofloxacin and ornidazole tablet dosage forms. the medium of dissolution used was 900 ml of 0.01n hcl, using a paddle apparatus at a stirring rate of 50 rpm. the drug release was evaluated by developed and validated spectroscopic methods. ofloxacin and ornidazole showed 293.4 and 319.6nm as λmax in 0.01n hcl. the methods were validated to meet requirements for a global regulatory filing. the validation included linearity, precision and accuracy. in addition, recovery studies and dissolution studies of three different tablets were compared and the results obtained show no significant difference among products.
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF OFLOXACIN AND ORNIDAZOLE IN TABLET DOSAGE FORM BY RP-HPLC  [PDF]
B.Dhandapani,,N.Thirumoorthy,Shaik Harun Rasheed,,M.Rama kotaiah
International Journal of Pharmaceutical and Biological Research , 2010,
Abstract: A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Ofloxacin and Ornidazole in combination. The separation was carried out using a mobile phase consisting of 2mM phosphate buffer and Acetonitrile with pH 3.5 adjusted with ortho phosphoric acid in the ratio of 70: 30%v/v. The column used was Phenomenex C18, (250 mm x 4.6 mm i.d, 5 m) with flow rate of 1 ml / min using PDA detection at 293 nm. The described method was linear over a concentration range of 5-50 g/ml and 12.5-125 g/ml for the assay of Ofloxacin and Ornidazole respectively. Gatifloxacin (50 g/ml) was used as nternal standard. The retention times of Ofloxacin, Ornidazole and Gatifloxacin were found to be 2.1, 2.5 and 5.5min respectively. Results of analysis were validated statistically and by recovery tudies. The limit of detection (LOD) and the limit of quantification (LOQ) for Ofloxacin and Ornidazole were found to be 5 and10 μg/ml10 and 25 μg/ml respectively. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Ofloxacin and Ornidazole bulk drug and in its pharmaceutical dosage form.
Method Development and Validation for The Simultaneous Estimation of Ofloxacin and Ornidazole in Tablet Dosage Form by Rp-Hplc
B.Dhandapani,,N.Thirumoorthy,Shaik Harun Rasheed,,M.Rama kotaiah
International Journal of Pharma Sciences and Research , 2010,
Abstract: A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Ofloxacin and Ornidazole in combination. The separation was carriedout using a mobile phase consisting of 2mM phosphate buffer and Acetonitrile with pH 3.5 adjusted with ortho phosphoric acid in the ratio of 70: 30%v/v. The column used was Phenomenex C18, (250 mm x 4.6 mm i.d, 5 m) with flow rate of 1 ml / min using PDA detection at 293 nm. The described method was linear over a concentration range of 5-50 g/ml and 12.5-125 g/ml for the assay of Ofloxacin and Ornidazole respectively. Gatifloxacin (50 g/ml) was used as internal standard. The retention times of Ofloxacin, Ornidazole and Gatifloxacin were found to be 2.1, 2.5 and 5.5min respectively. Results of analysis were validated statistically and by recovery studies. The limit of detection (LOD) and the limit of quantification (LOQ) for Ofloxacin and Ornidazole were found to be 5 and10 μg/ml 10 and 25 μg/ml respectively. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Ofloxacin and Ornidazolebulk drug and in its pharmaceutical dosage form.
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