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Efeito de produtos químicos sobre a mancha bacteriana (Xanthomonas perforans) e na ativa??o de proteínas relacionadas à patogênese em tomateiro
Terumi Itako,Adriana; Batista Tolentino Júnior,Jo?o; Fernandes da Silva Júnior,Tadeu A.; Marcelo Soman,José; Carlos Maringoni,Antonio;
Idesia (Arica) , 2012, DOI: 10.4067/S0718-34292012000200011
Abstract: the present study aims to evaluate the effect of fungicides and antibiotics to control bacterial spot (xanthomonas perforans) in tomato, and the activation of pathogenesis-related proteins. hybrid tomato ap 529 was used to assess the severity of disease. the treatments consisted of spraying with acibenzolar-s-methyl, fluazinam, pyraclostrobin, pyraclostrobin + methiran, copper oxychloride, copper oxychloride and mancozeb + oxytetracycline, and inoculated and non-inoculated controls. after three days of treatment, all plants were inoculated with x. perforans (106 cfu / ml). leaf discs were collected for assessment of peroxidase, polyphenol oxidase, β-1,3 glucanase, phenylalanine ammonia lyase and protease. the area under the disease progress curve (audpc) was calculated with the data of severity. all treatments had reduced audpc compared to the inoculated control. fungicides acibenzolar-s-methyl, pyraclostrobin, and pyraclostrobin + methiran had more satisfactory results in reducing the severity of bacterial spot on tomato. the products based on pyraclostrobin together with acibenzolar-s-methyl induced enzymatic activities of peroxidase, polyphenoloxidase and β-1,3 glucanase, indicating that these products may be related to the induction of resistance to bacterial spot on tomato plants.
Identification of Genes Differentially Expressed between Resistant and Susceptible Tomato Lines during Time-Course Interactions with Xanthomonas perforans Race T3  [PDF]
Heshan Du, Wenhui Li, Yuqing Wang, Wencai Yang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0093476
Abstract: Bacterial spot caused by several Xanthomonas sp. is one of the most devastating diseases in tomato (Solanum lycopersicum L.). The genetics of hypersensitive resistance to X. perforans race T3 has been intensively investigated and regulatory genes during the infection of race T3 have been identified through transcriptional profiling. However, no work on isolating regulatory genes for field resistance has been reported. In this study, cDNA-amplified fragment length polymorphism technique was used to identify differentially expressed transcripts between resistant tomato accession PI 114490 and susceptible variety OH 88119 at 3, 4 and 5 days post-inoculation of the pathogen. Using 256 selective primer combinations, a total of 79 differentially expressed transcript-derived fragments (TDFs) representing 71 genes were obtained. Of which, 60 were up-regulated and 4 were down-regulated in both tomato lines, 4 were uniquely up-regulated and 2 were uniquely down-regulated in PI 114490, and 1 was specifically up-regulated in OH 88119. The expression patterns of 19 representative TDFs were further confirmed by semi-quantitative and/or quantitative real time RT-PCR. These results suggested that the two tomato lines activated partly similar defensive mechanism in response to race T3 infection. The data obtained here will provide some fundamental information for elucidating the molecular mechanism of response to race T3 infection in tomato plants with field resistance.
Sensibilidade a cobre, estreptomicina e oxitetraciclina em Xanthomonas spp. associadas à mancha-bacteriana do tomate para processamento industrial
Quezado-Duval Alice Maria,Gazzoto Filho Ademir,Leite Júnior Rui P.,Camargo Luis Eduardo A.
Horticultura Brasileira , 2003,
Abstract: Apesar de amplamente empregados em lavouras de tomate para processamento industrial no Brasil, fungicidas cúpricos e antibióticos registrados para uso agrícola nem sempre resultam em controle eficiente das bacterioses que afetam a cultura. O aparecimento de estirpes resistentes é uma das causas dessa baixa eficiência. Avaliou-se, in vitro, a sensibilidade a cobre, estreptomicina e oxitetraciclina de 389 isolados de Xanthomonas spp. associadas à mancha-bacteriana do tomateiro, sendo 92 de X. axonopodis pv. vesicatoria (60 do grupo "A"/ra a T1 e 32 do "C"/ra a T3), 93 de X. vesicatoria (grupo "B"/ra a T2) e 204 de X. gardneri (grupo "D"/ra a T2). Os isolados foram obtidos de plantas doentes em campos comerciais de tomate para processamento industrial nos estados de Goiás, Minas Gerais, Pernambuco e Bahia, nos anos de 1995 a 1998 e em 2000. Alíquotas de 5 ml de suspens es bacterianas foram depositadas em meio Nutriente-ágar suplementado com sulfato de cobre, nas concentra es de 50 e 200 μg/ml; sulfato de estreptomicina, a 25 e 200 μg/ml e cloridrato de oxitetraciclina, a 25 μg/ml. Nenhum isolado foi resistente a oxitetraciclina, como também nenhum foi resistente ao cobre na concentra o de 200 μg/ml do sulfato de cobre. No entanto, houve diferen a entre isolados quanto à sensibilidade ao sulfato de cobre na concentra o de 50 μg/ml e ao sulfato de estreptomicina nas duas concentra es empregadas. As freqüências de isolados de X. gardneri, X. axonopodis pv. vesicatoria (grupos "A" e "C") e X. vesicatoria resistentes à estreptomicina (25 μg/ml do produto usado) foram, respectivamente, 98%, 38% e 2%, ao passo que, ao cobre, foram, respectivamente, 48%, 4% e 74%. Todos os isolados do grupo "C" foram sensíveis à estreptomicina e 97% sensíveis ao cobre.
Penicillamine induced pseudoxanthoma elasticum with elastosis perforans serpiginosa
Rath Namita,Bhardwaj Amit,Kar Hemant,Sharma P
Indian Journal of Dermatology, Venereology and Leprology , 2005,
Abstract: Long term D-penicillamine therapy, especially when used to treat Wilson′s disease has been shown to cause elastosis perforans serpiginosa, pseudoxanthoma elasticum perforans and other degenerative dermatoses. We report a 23-year-old male patient who presented with multiple firm papules, nodules over the neck, axillae, front of elbows for five years. He was a known case of Wilson′s disease on long-term treatment with penicillamine for the past 12 years. The papulonodular lesions were non-tender and some were discrete while others were arranged in a circinate pattern. There was central scarring of the skin within the circinate lesions. In addition, there were several small yellowish papules on both sides of the neck which eventually became confluent to form plaques. Histopathology confirmed the diagnosis of elastosis perforans serpiginosa and pseudoxanthoma elasticum. He was treated with cryotherapy (using liquid nitrogen through cryojet) for former lesions. The lesions showed remarkable improvement after five sittings. Now the patient is under trientine hydrochloride (750 mg twice daily) for Wilson′s disease.
Skin vascularisation field by the ascending branch of the peroneal artery ramus perforans  [PDF]
Ga?evi? Milomir,Milisavljevi? Milan,Novakovi? Marijan,Vojvodi? Danilo
Vojnosanitetski Pregled , 2011, DOI: 10.2298/vsp1107575g
Abstract: Background/Aim. Soft tissue defects in the distal third of the lower leg are persistent and constitute a major problem in the reconstructive surgery. This study presents an analysis of the anatomical vascularization filed of ascending branch of the peroneal artery ramus perforans (PARS). The aim of this study was to assess reliability of the distal flap on the antero-lateral aspect of a lower leg distal third. Methods. Direct gentiana violet injection into the interosseal perforator of ten fresh cadaveric lower legs with subsequent corrosion acrylic preparation was performed to reveal vascularization filed of the ascending branch of the PARP. Height, length, diameter and communication of perforating branch and its subsequent smaller ascending and descending branches were determined. The CAMIA software was used. Results. Our results show that the PARP is always present. Its origin from the peroneal artery is at the medial height of 66 mm when measured from the inferior border of the lateral malleolus. Medium length of ramus perforans is 51.7mm. After transition through the interosseous membrane, ramus perforans divides into ascending and descending branches. The diameter proximal to the level of bifurcation is 1.37 mm (variation 1.0-1.8 mm), and the diameter of the ascending branch distal to the level of bifurcation is 1 mm. Using CAMIA software, the medium length, width and area of the vascularization filed labeled with gentian violet were calculated to be 164 mm (variation 125-210 mm), 66 mm (57-77 mm), and 10,305 mm2 (6,385 mm2-14,341 mm2), respectively. Conclusion. Our results support the use of fasciocutaneous distal flap, vascularized by the ascending branch of the PARP for reconstruction of soft tissue defects in the distal third of the lower limb, malleolar regions and dorsum.
Granuloma anular perforans: Presentación de 1 caso Perforating granuloma annulare: A case report  [cached]
Aida Iris Uribe - Echevarría Delegado,Doris Joseph,Isora Montenegro Valera,Juan Hernández Vázquez
Revista Cubana de Medicina , 2004,
Abstract: Se describieron las características clínicas de una paciente a la cual le fue diagnosticado un granuloma anular perforans, entidad de muy baja incidencia por lo que consideramos muy útil la publicación del caso. La paciente acude a consulta por lesión única eritematopapulosa, de centro exulcerado, exudativo, en la pierna derecha. Después de realizar estudios complementarios e histopatológicos se llegó al diagnóstico de granuloma anular perforans; se impuso tratamiento sintomático y yoduro de potasio por vía sistémica, con el cual se logró una evolución satisfactoria The clinical characteristics of a female patient who was diagnosed a perforating granuloma annulare, an entity of very low incidence, whose publication is very useful, are described. The patient was seen at the physician′s office due to a unique erythematopapular lesion of ulcerated center, exudative in the right leg. After making the complementary and histopathological tests, it was obtained the above mentioned diagnosis. Symptomatic treatment was indicated and potassium iodide was given by systemic route and a satisfactory evolution was observed.
Meningitis due to Xanthomonas maltophilia.  [cached]
Girijaratnakumari T,Raja A,Ramani R,Antony B
Journal of Postgraduate Medicine , 1993,
Abstract: During 1st week of post-operative period, a 28 year old female patient operated for left cerebellopontine angle tumor, continued to get fever. Lumbar puncture did not reveal any organisms. She responded to ciprofloxacin. Two months later, she was readmitted with signs and symptoms of meningitis. The CSF tapped on lumbar puncture grew Xanthomonas maltophilia, Gram negative bacilli, sensitive to various antibiotics, ciprofloxacin being one of them. The patient was given ciprofloxacin for 3 weeks. On follow up, a year later she was found to be asymptomatic.
Elastosis Perforans Serpiginosa in a patient with Down syndrome treated with imiquimod 5% cream.  [cached]
Pernille Axèl Gregersen,Birgitte Stausb?l-Gr?n,Mette Ramsing,Mette Sommerlund
Dermatology Reports , 2010, DOI: 10.4081/dr.2010.e15
Abstract: Elastosis Perforans Serpiginosa (EPS) is a rare skin disease characterised by hyperkeratotic papules, transepidermal elimination of abnormal elastic fibres, and focal dermal elastosis. The aetiology is unknown, but an association with underlying systemic disorders, including Down syndrome has been describ-ed. Treatment is often difficult. A 45-year old man with Down syndrome presented with symmetrical annular elements on forearms and femora. The elements were erythematous with atrophic hypopigmented central healing and peripherally, infiltrated keratotic papules with desquamation. A punch biopsy showed the classical histopathologic features of EPS. We found no clinical signs of cerebrovascular or cardiovascular disease. We initiated topical therapy with imiquimod 5% cream once a day for 6 weeks followed by 3 times weekly for 4 weeks to a single element. As regression of EPS was observed and the patient tolerated the therapy well, treatment of other lesions was commenced, and further regression was seen.
Eficiência de uso da radia o fotossinteticamente ativa pela cultura do tomateiro em diferentes ambientes
Radin Bernadete,Bergamaschi Homero,Reisser Junior Carlos,Barni Nídio Antonio
Pesquisa Agropecuária Brasileira , 2003,
Abstract: A produ o de biomassa pelas culturas está relacionada à quantidade de radia o fotossinteticamente ativa interceptada e absorvida pelas folhas, bem como à eficiência com que estas convertem a energia radiante em energia química, pela fotossíntese. O objetivo deste trabalho foi avaliar a eficiência de uso da radia o fotossinteticamente ativa pelo tomateiro (Lycopersicon esculentum Mill.) cultivado em diferentes ambientes. Os experimentos foram realizados em estufa de plástico com e sem tela lateral antiinsetos e fora da estufa, em duas épocas (primavera-ver o e ver o-outono), no ano agrícola de 1999/2000. Mediu-se a matéria seca aérea e o índice de área foliar ao longo dos dois ciclos, assim como os fluxos de radia o incidente e transmitida. O ambiente em estufa com tela lateral antiinsetos teve menos radia o incidente e maior eficiência de seu uso: 0,44 e 0,60 g de matéria seca mol-1, nas primeira e segunda épocas, respectivamente. No ambiente fora da estufa, com mais radia o incidente, houve menor eficiência de seu uso (0,30 e 0,32 g mol-1), enquanto no ambiente em estufa sem tela lateral antiinsetos, foram obtidos valores intermediários de eficiência de uso da radia o (0,45 e 0,53 g mol-1).
Characterization of Xanthomonas spp. strains by bacteriocins
Bonini, Marcel;Maringoni, Antonio Carlos;Rodrigues Neto, Julio;
Summa Phytopathologica , 2007, DOI: 10.1590/S0100-54052007000100003
Abstract: twenty-five strains of xanthomonas axonopodis pv. citri and 14 strains of xanthomonas spp. were tested for bacteriocin production. x. axonopodis pv. passiflorae strains were sensitive to the bacteriocins produced by the 25 x. axonopodis pv. citri strains evaluated in this study while strains of x. axonopodis pv. manihotis and x. campestris pv. campestris showed variable sensitivity. only five of the 25 x. axonopodis pv. citri strains were not inhibited by the bacteriocins produced by the two x. axonopodis pv. passiflorae strains. the bacteriocins produced by the xanthomonas axonopodis pv. citri (fdc-806) and x. axonopodis pv. passiflorae (mar-2850 a) strains were thermolabile, resistant to lysozyme and sensitive to dnase. the bacteriocin produced by x. axonopodis pv. passiflorae was resistant to the action of proteinase k, trypsin and rnase while the bacteriocin produced by x. axonopodis pv. citri was sensitive to these enzymes. the bacteriocins produced by x. axonopodis pv. passiflorae and x. axonopodis pv. citri were called passifloricin and citricin, respectively.
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