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Detección de Helicobacter pylori y caracterización del la región -31 del gen de la Interleucina 1- humana en pacientes de una población colombiana con enfermedades gastroduodenales Helicobacter pylori detection and human Interleukin 1- genotyping in Colombian patients affected by gastroduodenal diseases
María Camila Montealegre Ortiz,Carlos Jaramillo Henao,Mabel Elena Bohórquez Lozano,Gustavo Montealegre Lynett
Revista Colombiana de Gastroenterologia , 2008,
Abstract: El propósito de este estudio fue investigar la presencia de Helicobacter pylori, realizar la caracterización del polimorfismo -31 del gen de la interleucina 1-β humana y establecer si existe asociación entre la presentación de alguno de los genotipos y la severidad de las manifestaciones clínicas en una población colombiana. Se analizaron biopsias gástricas provenientes de 71 pacientes, mediante histopatología y métodos moleculares (PCR y PCR-RFLP). Los resultados mostraron que el 97,2% de los pacientes presentaba alteraciones de la mucosa gástrica. La presencia de H. pylori se demostró en 44 pacientes y la frecuencia de los genotipos de IL-1B: TT, CT y CC fue de 12,7%, 60,6% y 26,8% respectivamente. No se encontró asociación entre los genotipos de la región polimórfica -31 del gen IL-1B y el grado de alteración de la mucosa gástrica en la población estudiada. The aim of this study was to investigate the presence of Helicobacter pylori and interleukin 1-β genotyping (IL-1B -31), to determine the relationship between one polymorphism in the -31 region of interleukin 1-β gene and the severity of the clinical manifestations. Gastric biopsies from 71 patients were submitted to histopathological examination and genotyped by molecular methods (PCR y PCR-RFLP). The results indicated that 97.2% of the patients have alterations in the gastric mucosa, the presence of H. pylori was demonstrated in 44 patients and TT, CT and CC genotypes were detected in 12.7%, 60.6% and 26.8%, respectively. IL-1B genotypes were not associated with the severity of gastric mucosal damage in the studied population.
Helicobacter pylori en pacientes con diferentes enfermedades gastroduodenales
Martínez Echavarría,María Teresa; Ferreira Capote,Raúl; González Torres,Maximino;
Revista Cubana de Medicina , 2008,
Abstract: the role played by helicobacter pylori in the development of different digestive diseases has been widely studied and discussed. the presence of this bacterium in biopsy samples obtained by endoscopy was studied. 69 patients with duodenal ulcer, gastric ulcer, chronic gastritis and dyspepsia were investigated. the diagnoses of gastric ulcer and chronic gastritis were histologically confirmed. 27 duodenal ulcers, 12 gastric ulcers, 24 chronic gastritis and 6 dyspepsias were analyzed. the presence of helicobacter pylori was detected through the amplification of a fragment of the ure a gene by polymerase chain reaction in 100 % of the duodenal ulcers, in 100 % of the gastric ulcers, in 83 % of the dyspepsias and in 92 % of chronic gastritis, for a total prevalence of 95.7 %.
Infecciones por helicobacter pylori Helicobacter pylori infections
Liliam Alvarez Gil
Iatreia , 1994,
Abstract: Se revisan los conocimientos sobre el papel de Helicobacter pylori en varias enfermedades gastroduodenales como la gastritis crónica (GC), úlcera gástrica (UG), úlcera duodenal (UD) y dispepsia no ulcerosa (DNU). La revisión abarca aspectos históricos, microbiológicos, clínicos, epidemiológicos, diagnósticos de laboratorio, terapéuticos y de patogénesis. The current knowledge of the role of Helicobacter Pylori in several gastroduodenal diseases is reviewed. It includes chronic gastritis, gastric and duodenal ulcers and nonulcerous dyspepsia. The following aspects are treated in this paper: history, microbiology. Clinical presentation, epidemiology, laboratory diagnosis, therapy and pathogenesis.
Frecuencia de los genotipos babA2, oipA y cagE de Helicobacter pylori en pacientes colombianos con enfermedades gastroduodenales
Quiroga,Andrés Javier; Diana Marcela,Cittelly; Bravo,María Mercedes;
Biomédica , 2005,
Abstract: introduction. helicobacter pylori infection is associated with the development of several gastroduodenal diseases. bacterial virulence genes have been found associated with an increased risk for gastric disease. objectives. herein, associations were made between the presence of vaca, caga, cage, baba2 and oipa genes in h. pylori isolates and the range of clinical consequences of the infection. methods. pcr was used to amplify vaca, caga, cage, baba2 and oipa genes in 166 isolates- 50 patients with peptic ulcer, 39 with non-atrophic gastritis, 26 with atrophic gastritis, 26 with intestinal metaplasia and 25 with gastric adenocarcinoma. results. caga, cage, baba2 and oipa genes were found in 73%, 75%, 48% and 74% of isolates, respectively. the cytotoxic vaca s1m1/ caga positive/ cage positive genotype was present in 64% (100/157) of isolates. a higher frequency of cytotoxic strains was observed in cancer patients (84%), intestinal metaplasia (91%) and peptic ulcer (81%) in comparison with gastritis patients (50%) (p=0.002, 0.008, 0.007, respectively). the oipa and baba2 frequency was higher in cytotoxic isolates than in non-cytotoxic isolates ( oipa: 81% vs. 52%, p=0,003; baba2: 58% vs. 12% (p<0.001). no significant association was found among clinical outcomes and oipa or baba2 genotypes, analyzed alone or in combination with vaca and caga. conclusion. therefore, baba2 or oipa genes are not marker indicators of ulcer or cancer.
Helicobacter pylori y lesiones endoscópicas e histológicas en mucosa gástrica de pacientes de 50 a os y más Helicobacter pylori and the endoscopic and histological lesions in the gastric mucosa of patients aged 50 and over
Felipe Pi?ol Jiménez,Manuel Paniagua Estévez,Bienvenido Gra Oramas,M Reyes de la Cruz
Revista Cubana de Medicina , 2008,
Abstract: Mundialmente es aceptado que la infección por Helicobacter pylori se incrementa con la edad y alcanza niveles entre 40 y 60 % en sujetos asintomáticos y 70 % en pacientes sintomáticos mayores de 50 a os con enfermedades gastroduodenales. Teniendo en cuenta la relación entre la infección por Helicobacter pylori y las enfermedades gastroduodenales se cifran prevalencias e incidencias en aumento sostenido con la edad (50-70 % en mayores de 50 a os) y se considera el significado aumento de la población cubana por encima de esa edad. Se realizó un estudio prospectivo, descriptivo en el Instituto de Gastroenterología, para conocer la frecuencia de este microorganismo, así como la presencia de lesiones en la mucosa gástrica en pacientes de 50 y más a os de edad. Se concluyó que el 69,6 % de los pacientes tuvieron resultados positivos al Helicobacter pylori por test de ureasa; de los 132 fragmentos de muestras tomados para biopsias de la mucosa gástrica, el 37,8 % fueron positivas para Helicobacter pylori, de ellas, el 25,7 % se localizó en la región antral. Se realizaron 68 diagnósticos endoscópicos en los pacientes con Helicobacter pylori positivos por test de ureasa; 64,7 % correspondió a gastritis crónica agudizada al nivel antral, seguido en orden de frecuencia por pangastritis, gastritis crónica agudizada al nivel del cuerpo, úlcera gástrica y úlcera duodenal. No se presentó lesión endoscópica de aspecto maligno en ningún paciente. Al estudiar la densidad de colonización de Helicobacter pylori en los 84 diagnósticos histológicos realizados, 69,0 % presentó densidad ligera; 29,9 %, moderada y 1,1 %, severa. Se observó una tendencia hacia la densidad de colonización ligera a medida que progresaba la lesión histológica. Se sugirió continuar el estudio con mayor número de pacientes para caracterizar el comportamiento de la infección en los pacientes de 50 a os y más, y así tomar medidas preventivas encaminadas a erradicar la bacteria y mejorar la calidad de vida. It is accepted all over the world that Helicobacter pylori infection increases with age and reaches levels between 40 and 60 % in asymptomatic individuals, and of 70 % in symptomatic patients over 50 with gastroduodenal diseases. Taking into account the relation existing between Helicobacter pylori infection and the gastroduodenal diseases, prevalences and incidences showing a sustained increase with age (50-70 % in patients over 50) are ciphered, and the significant growth of the Cuban population above this age is considered. A prospective and descriptive study was undertaken at the Institute of Gast
Citocinas, gastritis crónica y Helicobacter Pylori Cytokines, chronic gastritis and Helicobacter pylori
Felipe Pi?ol Jiménez,Manuel Paniagua Estévez
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: El Helicobacter pylori es una bacteria curva, gramnegativa, que exclusivamente habita en la mucosa gástrica. Desde su descubrimiento y caracterización ha sido implicada en la fisiopatología de las enfermedades gastroduodenales, que incluyen gastritis, úlcera péptica, carcinoma gástrico, y linfoma MALT, dando origen a numerosas hipótesis que tratan de explicar los diferentes eventos que ocurren en el proceso inflamatorio del estómago a su llegada, caracterizado por una marcada infiltración de células inflamatorias (neutrófilo, monocitos, linfocitos y otras), que al ser activadas liberan localmente varios mediadores químicos, responsables del da o tisular; se destacan las citocinas como mediadores importantes de tal proceso. Se realiza una revisión actualizada de las diversas funciones biológicas de las citocinas en el da o tisular de la mucosa gástrica The Helicobacter pylori is a curve, Gram-negative bacteria that lives exclusively in the gastric mucosa. Since its discovery and characterization it has been involved in the physiopathology of gastroduodenal diseases, including gastritis, peptic ulcer, gastric carcinoma and MALT lymphoma. This has given rise to numerous hypotheses that try to explain the different events that occur in the inflammatory process of the stomach on its arrival, characterized by a marked infiltration of inflammatory cells (neutrophils, monocytes, lymphocytes and others) that once activated release locally various chemical mediators, which are responsible for the tissue damage. Cytokines outstand as important mediators of such a process. An updated review of the different biological functions of cytokines in the tissue damage of the gastric mucosa is made
Detección de Helicobacter pylori y caracterización del la región -31 del gen de la Interleucina 1-? humana en pacientes de una población colombiana con enfermedades gastroduodenales
Montealegre Ortiz,María Camila; Jaramillo Henao,Carlos; Bohórquez Lozano,Mabel Elena; Montealegre Lynett,Gustavo; Delgado,María del Pilar;
Revista Colombiana de Gastroenterologia , 2008,
Abstract: the aim of this study was to investigate the presence of helicobacter pylori and interleukin 1-β genotyping (il-1b -31), to determine the relationship between one polymorphism in the -31 region of interleukin 1-β gene and the severity of the clinical manifestations. gastric biopsies from 71 patients were submitted to histopathological examination and genotyped by molecular methods (pcr y pcr-rflp). the results indicated that 97.2% of the patients have alterations in the gastric mucosa, the presence of h. pylori was demonstrated in 44 patients and tt, ct and cc genotypes were detected in 12.7%, 60.6% and 26.8%, respectively. il-1b genotypes were not associated with the severity of gastric mucosal damage in the studied population.
HELICOBACTER PYLORI SEROLOGY AND EVALUATION OF GASTRODUODENAL DISEASE IN NIGERIANS WITH DYSPEPSIA
JA Otegbayo, OA Oluwasola, A Yakubu, GN Odaibo, OD Olaleye
African Journal of Clinical and Experimental Microbiology , 2004,
Abstract: Helicobacter pylori (H. pylori) has been strongly associated with various gastroduodenal diseases worldwide with only a few studies emanating from developing countries. The objectives of this study were to determine the prevalence of serum Immunoglobulin G (IgG) and underlying gastroduodenal pathology in Nigerian patients with dyspepsia and ascertain the usefulness of H. pylori IgG screening in decreasing endoscopic workload in dyspeptics in Nigeria. Fifty-five patients with dyspepsia and 55 age and sex-matched apparently normal control were screened for H. pylori IgG using ImmunocombRII kits. Each of the 55 patients was also examined endoscopically with biopsies taken appropriately. Serology was positive in 94.5% and 92.7% of dyspeptic patients and controls respectively. Gastroduodenal inflammation was the commonest endoscopic finding, 43 (78.18%). Other findings were malignant gastric tumour 6(10.9%), reflux oesophagitis 3 (5.45%), gastric ulcer 2 (3.64%), and duodenal ulcer in 1 (1.82%). Chronic gastritis was the main histopathologic finding in the dyspeptic patients. It is concluded that serum H. pylori IgG cannot be used as a screening procedure to reduce endoscopic workload in Nigerian patients with dyspepsia. Key Words: dyspepsia; Helicobacter pylori serology; gastroduodenal disease African Journal Of Clinical And Experimental Microbiology Jan 2004 Vol.5 No.1 131-138
Helicobacter Pylori Infection and Benign Gastroduodenal Diseases, Data from the Trakya Region
Hasan üM?T,Gülbin üNSAL,Ahmet TEZEL,Ali R?za SOYLU
Trakya Universitesi Tip Fakultesi Dergisi , 2010,
Abstract: Objective: Helicobacter pylori (H. pylori) is implicated in the etiology of gastric and duodenal ulcer, non-ulcer dyspepsia, atrophic gastritis, gastric adenocarcinoma and mucosa associated lymphoid tissue lymphoma (MALToma). The aim of our study is to evaluate the prevalance of H. pylori infection in patients with benign gastroduodenal diseases. Material and Methods: H. pylori infection was evaluated retrospectively with the urease test in 4714 patients with benign gastroduodenal disorders who applied to the endoscopy unit of Trakya University Hospital, the only University hospital in the Trakya region, between November 2003 and October 2007 with dyspeptic complaints.Results: Overall, the rapid urease test was positive in 52.8% of cases. Helicobacter pylori was positive in 65% of the bulbar ulcer, 61% of the erosive bulbitis, 60.2% of the gastric ulcer and, 48.4% of the gastritis patients. (p<0.001). H. pylori positivity was 52% in Trakya born patients, 56.3% in immigrants from Anatolia and 48.7% in immigrants from Europe (p=0.02). H. pylori positivity frequencies in women and men were 52.2% and 53.2%, respectively (p=0.52). Urease test positivity was significantly more frequent in patients 30-60 years of age compared to the groups younger than 30 or older than 60.Conclusion: The epidemiologic characteristics of H. pylori infection shows features of both Turkey and neighbouring countries of Europe.
Gastroduodenal lesions in peritoneal dialysis patients: the role of Helicobacter pylori infection  [cached]
Ibrahiem Saeed Abdulrahman,Abdullah K. Al-Hwiesh
Nephrology Reviews , 2012, DOI: 10.4081/nr.2012.e3
Abstract: The aim of this study was to examine the prevalence of Helicobacter pylori infection and the associated gastroduodenal lesions in peritoneal dialysis patients. Ninety-eight patients with dyspeptic complaints were included in the study. They were divided into two groups; group 1 consisted of 48 patients with endstage renal disease (ESRD) on PD and group 2 (control) of 50 patients without renal disease. All patients were subjected to upper gastrointestinal endoscopies, and gastric biopsies were obtained for histological evidence of H.pylori infection. Anti-H.pylori IgG antibodies were tested in the PD patients using the ELISA test. The mean age of both groups was similar. Twenty-three (47.9%) of the 48 patients on PD and 25 (50.0 %) of the 50 nonrenal disease patients were positive for H.pylori infection. There was no significant difference in H.pylori prevalence between the two groups (P>0.05). No correlation was found between H.pylori infection and age, sex, primary disease, or type (CAPD or APD). However, prevalence was significantly higher (30.4% vs 17.4%, P<0.001) in patients who were on PD for less than 24 months. False positive serology for H.pylori in the absence of positive histology was found in 7 out of the 25 negative cases (28.0 %) in the dialysis group. The prevalence of duodenal ulcers was significantly higher in H.pylori positive than in H.pylori negative ESRD patients on PD (18.8% vs 8.3%, P<0.05). Gastroesophageal reflux disease (GERD) was seen in 21 (43.8%) patients in group 1 and in 8 (16%) cases in group 2 (P<0.01). GERD was, however, significantly lower in H.pylori positive patients in both groups (12.5% vs 31.3% in group 1 and 4% vs 12% in group 2, P<0.001 and P<0.01, respectively). In conclusion, long-term PD seems to decrease the prevalence of H.pylori infection. Testing H.pylori antibodies lacks specificity for diagnosing active H.pylori infection in PD patients. H.pylori infection in patients on PD is associated with increased risk of gastroduodenal lesions and decreased prevalence of GERD.
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