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A Reliable and High Yielding Method for Isolation of Genomic DNA from Ammi majus  [PDF]
Sandip S. Magdum
International Research Journal of Biological Sciences ,
Abstract: The developed protocol describes a cheaper, quicker and reliable method for the isolation of pure DNA from medicinal herbs, such as Ammi majus, which produces the secondary metabolites xanthotoxin and berganpectane having immense medicinal importance. Use of CTAB, liquid nitrogen and EDTA in different isolation protocols analyzed for A. majus, all were ended with polysaccharide and protein contamination with low purity of DNA (A260/280 = 1.3 – 1.6), revealed a need for method modification for the inexpensive and rapid isolation of pure DNA. Developed reliable and competent protocol isolated enough pure DNA (A260/280 = 1.81) without following time consuming lengthy steps and hazardous chemicals used in other protocols, which increase experimental costs, risk, and need expertise to perform. The explained protocol requires few chemicals and little time to obtain pure DNA having yield 688 μg/g of A. majus. A higher quantity of isolated DNA obtained from young fresh leaf samples than from either the callus or stem. A. majus is a pharmaceutically important medicinal herb, and the present protocol aids in the analysis and modification of its genes.
V. R. Mohan et al.
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: The present study was designed to screen and evaluate the hepatoprotective and antioxidant activity of ethanol extracts of leaf and bark of Naringi crenulata (Roxb) Nicolson (NCL, NCB) against CCl4 induced hepatotoxicity in rats. Liver functions were assessed by the activities of liver marker enzymes, SGOT, SGPT, ALP, total protein, albumin, globulin, total, conjugated and unconjugated bilirubins. It also exhibited antioxidant activity by showing the increased activity of SOD, CAT, GPx and GRD and decreased in TBARS compared to CCl4 treated groups. Silymarin, a known hepatoprotective drug is used for comparison. The plant extracts were effective in protecting liver against injury induced by CCl4 in rats.
Hepatoprotective Action of Radix Paeoniae Rubra Aqueous Extract against CCl4-Induced Hepatic Damage  [PDF]
Ruidong Li,Wenyuan Guo,Zhiren Fu,Guoshan Ding,You Zou,Zhengxin Wang
Molecules , 2011, DOI: 10.3390/molecules16108684
Abstract: In the present study the capacity of Radix Paeoniae Rubra aqueous extract (RPRAE) as an antioxidant to protect against carbon tetrachloride (CCl4)-induced oxidative stress and hepatotoxicity in Wistar rats was investigated. Six groups of rats were used. Radix Paeoniae Rubra aqueous extract (100 or 200 or 300 mg/kg of bw) or bifendate (100 mg/kg of bw) were given daily by gavage to the animals on 28 consecutive days to elucidate the protective effects against CCl4-induced hepatotoxicity. The 20% CCl4/olive oil was gavage of gastric tube twice a week (on the third and seventh days of each week). The animals of normal control group were given only vehicle. The animals of CCl4-treated group were administered with CCl4 twice a week (on the third and seventh days of each week) and with vehicle on rest of the days. The test materials were found effective as hepatoprotective agents, as evidenced by plasma and liver biochemical parameters. Therefore, the results of this study show that Radix Paeoniae Rubra aqueous extract can protect the liver against CCl4-induced oxidative damage in rats, and the hepatoprotective effects might be correlated with its antioxidant and free radical scavenger effects.
Thangakrishnakumari S,Nishanthini A,Muthukumarasamy S,Mohan V.R.
International Journal of Research in Ayurveda and Pharmacy , 2012,
Abstract: The aim of the study was to investigate the hepatoprotective activity of ethanol extract of whole plant of Canscora perfoliata in CCl4 induced hepatotoxic rats. Administration of hepatotoxins (CCl4) showed significant elevation of serum SGOT, SGPT, ALP, bilirubin, conjugated, unconjugated bilirubin and lipid peroxidation. Treatment with Canscora perfoliata (150 and 300mg/kg) significantly reduced the above mentioned parameters. The plant extract also enhanced the antioxidant activity. The ethanol extract of Canscora perfoliata have significant effect on the CCl4 induced hepatotoxicity animal models.
Hepatoprotective Activity of Livobond A Polyherbal Formulation Against CCl4 Induced Hepatotoxicity in Rats  [PDF]
Usha S. Satyapal,Vilasrao J. Kadam,Rumi Ghosh
International Journal of Pharmacology , 2008,
Abstract: In the present study, Livobond was evaluated for its hepatoprotective effects against carbon tetrachloride-induced hepatocellular injury in rats. Hepatotoxicity was induced in male Sprague-Dawley rats by intraperitoneal injection of CCl4 (1.5 mL kg-1) in olive oil (1:1). Livobond at a dose of 500 and 750 mg/kg/day and silymarin standard 50 mg/kg/day was administered orally for 7 days. The hepatoprotective effect of Livobond and standard was evaluated by the assay of biochemical parameters viz., alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), total and direct bilirubin, liver lipid peroxidation, total proteins, catalase and by histopathological studies of the liver. The toxic effects of CCl4 in Livobond treated group was controlled significantly by restoration of the levels of serum bilirubin, proteins and enzymes as compared to the CCl4 treated and silymarin treated groups. Histopathological studies further confirmed the hepatoprotective activity of Livobond. The results suggest that Livobond is able to significantly alleviate the hepatotoxicity induced by CCL4 and may be attributed to the antioxidant property of the formulation.
Hepatoprotective Effect of the Ethanolic Extract of Urtica parviflora Roxb. in CCl4 Treated Rats  [PDF]
Prasanna Kumar Kar,Lilakanta Nath,Suvakanta Dash,L. Sutharson
International Journal of Pharmacology , 2007,
Abstract: The ethanolic extract of leaves of Urtica parviflora (EEUP) was evaluated for the hepato protective effect in carbon tetrachloride (CCl4) induced hepatotoxicity in rats to prove its ethnomedicinal claim by the hill people of Sikkim. Hepatotoxicity was induced in Swiss Albino male rats of Sprague Dawley strain by subcutaneous injection of carbon tetrachloride at the dose of 1 mL kg-1 body weight. The hepatoprotective activity was evaluated by the assay of liver function biochemical parameters such as aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total bilirubin, serum protein and by study of histopathology of the livers. The toxic effect of carbon tetrachloride was controlled significantly by the EEUP at 250, 500 and 700 mg kg-1 p.o. (p<0.05) as compared to the CCl4 treated animals by restoration of the levels of serum bilirubin, proteins and hepato protective enzymes. Histopathological studies revealed that the centrolobular necrosis induced by CCl4 was recovered to normal state by EEUP in a dose dependent manner. The study confirms the possible hepatoprotective potentiality of the ethanolic extract of leaves of Urtica parviflora which had been collected from Sikkim. Studies are under process to isolate and characterize the bioactive component present in the plant as well as to establish the mechanism of action underlying for its hepatoprotective potentiality.
Evaluation of Hepatoprotective Activity of Argemone mexicana Aerial Part Extracts on CCL4 Induced Liver Damage in Wistar Rats  [cached]
S.I.Y. Adam,I.A. Idris,E.H. Abdelgadir,E.E. Kamal
British Journal of Pharmacology and Toxicology , 2011,
Abstract: This study is carried out on Wistar rats to evaluate the hepatoprotective activity of Argemone mexicana L aerial part extracts against CCL4 induced acute liver damages. The areal part of the plant was extracted by methanol and water using soxhlet, rotary evaporator and freeze drying apparatus. Hepatic injury was achieved by injecting 3 mL/kg s.c. of CCL4 in equal volume proportion with olive oil. The aqueous and methanol extracts of Argemone mexicana areal part were given at different doses, 100, 200, 400 mg/kg/day Methanol extract, and 400 mg/kg/day orally of aqueous extract for 5 days under CCL4 induction at 3rd day. The significance of differences between means was compared among the groups using Independent-sample T-test with probability value. The methanol extract at 100 mg/kg offered significant hepatoprotective activity (p<0.05) by reducing the serum marker enzyme, like Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyrovate Transaminase (SGPT) and Alkaline Phosphatase (ALP). Histopathlogical studies further confirmed the hepatoprotective activity of 100 mg/kg methanol extract. Argemone mexicana L. at dose 100 mg/kg indeed has a reasonable potential in healing liver parenchyma and regeneration of liver cells hence it may acts as a potent liver tonic. The results obtained were compared with silymarin (70 mg/kg.oral), the standard drug, which shown recovery toward normalization almost like that of silymarin, therefore, we recommend for further studies to isolate the pure component and the mechanism that displayed the hepatoprotective activity for making standard drug.
P Royal Frank,V Suresh,G Arunachalam,SK Kanthlal
International Research Journal of Pharmacy , 2012,
Abstract: Oxidative damage is involved in the pathogenesis of various hepatic injuries. In the present study, the protectivity of methanolic extract of leaves of Adiantum incisum forsk (MEAI), as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in Albino Wistar rats was investigated. Intraperitoneal injection of CCl4, produced a marked elevation in the serum levels of serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin, and decrease in the total protein. Histopathological analysis of the liver of CCl4-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GPx, LPO and CAT. Daily oral administration of methanolic extract of A.incisum forsk at 100 and 200 mg/kg doses for 10 days produced a dose-dependent reduction in the serum levels of liver enzymes. Treatment with A.incisum forsk normalized various biochemical parameters of oxidative stress and was compared with standard Silymarin. Therefore, the results of this study show that A.incisum forsk can be proposed to protect the liver against CCl4-induced liver damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenger effects.
Hepatoprotective Potential of Extracts from Seeds of Areca catechu and Nutgalls of Quercus infectoria  [PDF]
Pimolpan Pithayanukul,Saruth Nithitanakool,Rapepol Bavovada
Molecules , 2009, DOI: 10.3390/molecules14124987
Abstract: Aqueous extracts from seeds of Areca catechu L. (Arecaceae) (AC) and nutgalls of Quercus infectoria Oliv. (Fagaceae) (QI) were investigated for their hepatoprotective potential by studying their antioxidant capacity using four different methods, by determining their in vitro anti?inflammatory activity against 5-lipoxygenase, and by evaluating their hepatoprotective potential against liver injury induced by carbon tetrachloride (CCl4) in rats. AC and QI extracts exhibited potent antioxidant and anti-inflammatory activities. Treatment of rats with AC and QI extracts reversed oxidative damage in hepatic tissues induced by CCl4. It is suggested that extracts rich in either condensed or hydrolysable tannins and known for their potent antioxidant and anti-inflammatory activities, may potentially confer protection against oxidative stress?induced liver injury. These data should contribute to evidence-based traditional medicines for anti-inflammatory and hepatoprotective effects of both extracts.
Hepatoprotective Activity of Capparis spinosa Root Bark Against CCl4 Induced Hepatic Damage in Mice
Nasrin Aghel,Iran Rashidi,Amir Mombeini
Iranian Journal of Pharmaceutical Research , 2007,
Abstract: Many hepatoprotective herbal preparations have been recommended in alternative systems of medicine for the treatment of hepatic disorders. No systematic study has been done on protective efficacy of Capparis spinosa (Capparidaceae) to treat hepatic diseases. Protective action of C. spinosa ethanolic root bark extract was evaluated in this study in an animal model of hepatotoxicity, which was induced by carbon tetrachloride.Healthy male mice (30-35 g body weight, 6-8 week old) were divided into 7 groups. Group 1 was normal control group; Group 2, the hepatotoxic group was given CCl4; Group 3 was administered olive oil (vehicle); Groups 4-6 received different doses of ethanolic root bark extract (100, 200 & 400 mg/kg) with CCl4; Group 7 was administered overdose of the extract (800 mg/kg). The parameters studied were alanine transaminase and aspartate transaminase activities and duration of sleep. The hepatoprotective activity was also supported by histopathological studies of liver tissue.Results of the biochemical studies of blood samples of CCl4 treated animals showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by CCl4. Whereas blood samples from the animals treated with ethanolic root bark extracts showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells. The results revealed that ethanolic root bark extract of C. spinosa could afford significant dose-dependent protection against CCl4 induced hepatocellular injury.
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