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Isolation of Bacteriocin Producing Lactic Acid Bacteria from Fish Gut and Probiotic Activity Against Common Fresh Water Fish Pathogen Aeromonas hydrophila  [PDF]
P. Vijayabaskar,S.T. Somasundaram
Biotechnology , 2008,
Abstract: Lactic acid bacteria (LAB) produce many kinds of metabolites, which might affect the other microbes in the fish mid gut. Lactic acid produced by both homolactic acid and heterolactic strains, which will reduce pH in the luminal contents in the stomach of neonatal piglets of fish. Isolated LAB was used as a probiotic in fresh water fish tilapia (Oreochromis mossambicus) against the most common fish pathogen Aeromonas hydrophila. Higher antagonistic activity recorded from extra cellular protein (ECP) or bacteriocin compared to the intra cellular protein (ICP) against A. hydrophila. After feeding with the potential probiotics for 25 days, challenge by immersion indicated effectiveness at reducing disease caused by A. hydrophila in fishes. Tilapia exhibited no significant difference in growth, survival nor external appearance between the probiotic fed combine probiotic treatments, but significant differences (p< 0.05) occurred between probiotic and control groups. The use of LAB also enhances the production rate of rotifers, which act as biocarriers of probiotics and when fed to fish, they showed increased growth rate and weight of the animal. LAB is highly appreciated as a biological enhancers-probiotics, because of beneficial effect of live microorganisms
益生芽孢杆菌对草鱼肠上皮细胞的黏附及对嗜水气单胞菌的抑制
Adherence of Bacillus subtilis to intestinal epithelial cells of grass carp and its inhibition to Aeromonas hydrophila
 [PDF]

孔维光,吴志新,李思思,赵慧,李锡阁,陈孝煊
- , 2017,
Abstract: 为探讨益生性枯草芽孢杆菌对肠上皮细胞黏附及对嗜水气单胞菌的抑制作用,以草鱼肠上皮细胞为研究对象,检测了枯草芽孢杆菌对肠上皮细胞的黏附率、对嗜水气单胞菌的黏附抑制率和对各种生理生化指标的影响。结果显示:枯草芽孢杆菌对草鱼肠上皮细胞形态、细胞中四甲基偶氮唑盐(MTT)OD值、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)和谷草转氨酶(GOT)活性等各种生理指标无显著影响,但在孵育3 h 和 6 h 后分别显著降低了Na+,K+-ATP酶和谷丙转氨酶(GPT)的活性;与嗜水气单胞菌孵育 3 h 后,肠上皮细胞由椭圆变成不规则形态,培养液的死细胞增多,并且在多数时间点上显著降低了MTT OD值以及谷草转氨酶、Na+,K+-ATP酶和谷丙转氨酶的活性,细胞中 MTT OD值和细胞上清中乳酸脱氢酶的活性显著提高(P<0.05);枯草芽孢杆菌和嗜水气单胞菌共孵育对肠上皮细胞形态及各种生理指标的影响明显小于嗜水气单胞菌组;黏附抑制实验显示枯草芽孢杆菌可以显著降低嗜水气单胞菌对肠上皮细胞的黏附率。提示枯草芽孢杆菌可以抑制嗜水气单胞菌对草鱼肠上皮细胞的黏附,并减轻嗜水气单胞菌对细胞的损伤。
To investigate the adherence of Bacillus subtilis and its inhibitory of Aeromonas hydrophila, the adherence rate of B. subtilis, its inhibition rate of A. hydrophila, and various physiological and biochemical indexes were detected in the intestinal epithelial cells of grass carp. The results showed that after treatment of B. subtilis alone, cell morphology, methyl thiazolyl tetrazolium optical density (MTT OD) value, lactate dehydrogenase (LDH), alkaline phosphatase (AKP) and glutamic oxalacetic transaminase (GOT) activity were unsignificantly changed (P>0.05), but the activities of Na+, K+-ATPase and glutamic pyruvic transaminase (GPT) significantly increased (P<0.05) at 3 h and 6 h, respectively. The cell shape was obviously changed by A. hydrophila from ellipse to irregular shape and the number of dead cells in the culture medium increased. Meanwhile,A. hydrophila could significantly increase MTT OD value and LDH activity in the culture medium, and significantly decrease the activities of Na+,K+-ATPase, GOP and GPT in the cells (P<0.05). The adherence experiment showed that B. subtilis could significantly reduce the adhesion of A. hydrophila to grass carp intestinal cells. In brief, adhesion and damage of A. hydrophila to intestinal epithelial cells were inhibited by B. subtilis.
The Role of Aeromonas hydrophila in Diarrhea
MM Aslani,MY Alikhani
Iranian Journal of Public Health , 2004,
Abstract: Aeromonas hydrophila is a causative agent of a number of human infections. Aeromonads have been isolated from patients with diarrhea. In spite of a number of virulence factors produced by Aeromonas species, their association with diarrheal diseases has not been clearly linked. In current study, 1546 fecal samples of a randomly selected population were screened for presence of A. hydrophila. Out of the total number of cases, 20 were suffering from diarrhoea and the rest were asymptomatic healthy individuals. The result showed that 3.4% of the samples were positive for Aeromonas spp. A. hydrophila was isolated as the sole enteropathogen from 80% diarrheal and 20% asymptomatic cases. A significant association was found between the A. hydrophila and diarrhoeal diseases in rural and urban areas (P<0.05), while no difference was revealed between genders. The highest rates of A.hydrophila (3.9%) was detected in children <6 years of age. A significant association was also found between A. hydrophila and different age groups (P< 0.05). In conclusion, our epidemiological study showed that Aeromonas spp. as a sole enteropathogen could be responsible for diarrhea.
Meningitis due to aeromonas hydrophila  [cached]
Seetha K,Jose B,Jasthi A
Indian Journal of Medical Microbiology , 2004,
Abstract: A 3-month-old male child with the history of fever, of not sucking the breast and exhibitingsymptoms of meningitis was brought to the hospital for medical advice and was investigated. Aeromonas hydrophila was isolated thrice from CSF, blood samples as well as the water source.
Diarreas invasivas por Aeromonas hydrophila
Herrera,Marco L.; Vargas,Alvaro; Moya,Tatiana; Campos,Marlen; Marín,José Pablo;
Revista Médica del Hospital Nacional de Ni?os Dr. Carlos Sáenz Herrera , 2002,
Abstract: se presenta el hallazgo de ni?os con diarreas atribuibles a aeromonas hydrophila pero que presentan características de diarrea invasiva, contrastando con lo esperado para este agente donde el desarrollo de diarrea secretoria es lo esperado.
Identification of Aeromonas hydrophila infection with specific monoclonal antibodies  [PDF]
Paisarn Sithigorngul
Maejo International Journal of Science and Technology , 2007,
Abstract: Whole cell of Aeromonas hydrophila 1234 was used for immunization to produce monoclonal antibodies (MAbs). Three different groups of MAbs specific to Aeromonas were obtained. The first group of MAbs demonstrated high specificity and bound to the A. hydrophila 1234 only but did not bind to the other two A. hydrophila isolates. This group of MAbs bound to a series of lipo-polysaccharides (LPS) with molecular masses range from 10 to190 kDa. The second group of antibodies recognized A. hydrophila 1234 and 2798 isolates, and bound to a series of LPS with molecular masses range from 5-200 kDa. The third group of MAbs recognized all three isolates of A. hydrophila and two isolates of A. sobria, and lightly bound to A. caviae. This group of MAbs also bound to an unknown protein with molecular mass of 20 kDa. The MAbs in group 1 and group 2 can be used to detect the bacteria in tissues by immunohistochemistry. Both groups of MAbs bound to LPS at different sites in which the MAbs in group 2 bound to the side chain of O antigen while the MAbs in group 1 bound to the polymerization site at the core of oligosaccharide. All of the MAbs can be used to identify Aeromonas by dot blotting with the sensitivity range from 103-104 CFU/ l. This study demonstrated a convenient immunological tool that can be used for simple and accurate identification of A. hydrophila, as well as for diagnosis of the A. hydrophila infection in animals. This immunological tool can replace costly and laborious biochemical tests.
Virulence and cytotoxicity of seafood borne Aeromonas hydrophila
Illanchezian, Seethalakshmi;Jayaraman, SathishKumar;Manoharan, Muthu Saravanan;Valsalam, Saritha;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000400016
Abstract: the present study was conducted to determine the virulence and cytotoxicity of aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in chennai, tamil nadu, india. among 73 a. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on vero cells. cell shrinkage, detachment and rounding of vero cells were recorded as cytotoxic changes. only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with vero cells. positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. these results demonstrated the presence of wide spread, pathogenically characterized, cytotoxic seafood borne a. hydrophila in chennai.
BEHAVIOUR OF Aeromonas hydrophila IN SALTED SWORDFISH SAMPLES  [cached]
A Giuffrida,F Giarratana,E. Gervasi,M Bucca
Italian Journal of Food Safety , 2010, DOI: 10.4081/ijfs.2010.7.36
Abstract: A challenge test for Aeromonas hydrophila in salted swordfish samples was carried out. Particularly, 24 samples (250g) were experimentally contaminated, salted and stored at two different temperature regimes (fluctuating – F group - and non fluctuating – NF group – regime). The count of A. hydrophila, Enterobacteria and Lactic acid bacteria (LAB) as well as the determination of pH and aw were performed at 0, 19 43, 163, 187, 230, 320 and 368 hours whereas the temperature was monitored continuously by using 6 data-loggers. In both group, the mean concentrations of A. hydrophila did not exceed Log 3 cfu/g and decreased below the mean value of Log 1 cfu/g after 368 hours. However in the F group the A. hydrophila growth was slower and the decrease appeared slightly higher than NF group and this suggests the temperature fluctuations induces a more pronounced behaviour variability of A. hydrophila under stressing conditions.
Effect of certain phytochemicals on Aeromonas hydrophila
Vijay Kothari,,Madhu Naraniwal,Ankit Gupta
Research in Biotechnology , 2011,
Abstract: This study was aimed at investigating effect of different phytochemicals on growth ofAermonas hydrophila. Susceptibility of A. hydrophila to seven different phytocompoundswas evaluated by broth dilution assay. Among all the test compounds curcumin with aminimum inhibitory concentration of ~175 μg/ml was found to be most effective against A.hydrophila, followed by tannic acid. Gallic acid failed to show any effect on growth of thetest organism. A growth curve study in absence and presence of curcumin was also made.Since A. hydrophila is viewed as a challenging and notorious food-borne pathogen, anddifficult to control owing to its resistance to many antibiotics, it is required to find novelapproaches to control it. Our study found curcumin to be effective against this organism as abacteriostatic agent at microgram concentrations.
Multiple drug resistance in Aeromonas hydrophila isolates of fish
Kaskhedikar,M. and Chhabra,D
Veterinary World , 2010,
Abstract: Fourteen antibacterial agents belonging to 9 different groups of antibiotics viz. aminoglycosides, cephalosporins, nitrofurantoin, fluroquinolones, chloramphenicol, sulphonamides, tetracyclines, penicillin and polymixin were used for in vitro sensitivity testing of eight isolates of Aeromonas hydrophila isolated from fifteen samples of fish, collected from retail shops in Mhow city. The sensitivity (100%) was attributed to ciprofloxacin, cefuroxime, ceftriaxone, cephotaxime, chloramphenicol, gentamycin, kanamycin, nitrofurantoin, nalidixic acid and ofloxacin followed by Co-trimoxazole (62.2%) and oxytetracycline (50%). All the isolates were resistant to ampicillin and colistin antibiotics. That means, none of the isolates were found to be sensitive for penicillin and polymixin group of antibiotics. Multiple drug resistance was also observed in all A. hydrophila isolates. [Vet. World 2010; 3(2.000): 76-77]
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