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洛阳市牡丹灰霉病病原菌的鉴定
Identification of gray mold pathogens on tree peony Paeonia suffruticosa in Luoyang
 [PDF]

杨瑞先,刘萍,王祖华,宋根娣,Yang Ruixian,Liu Ping,Wang Zuhua,Song Gendi
- , 2017, DOI: 10.13802/j.cnki.zwbhxb.2017.2016029
Abstract: 为明确洛阳市牡丹灰霉病的病原菌种类,于2015年从该地区的4个牡丹Paeonia suffruticosaAndr.种植区采集灰霉病样品,采用组织分离法对病原菌进行分离和纯化,结合形态学和基因序列分析对分离物进行鉴定,采用针刺接种法测定不同分离物对牡丹离体叶片的致病性。结果表明,从灰霉病样品中分离获得40株分离物,这些分离物分别属于I、Ⅱ、Ⅲ型菌,I型菌不易产孢但产菌核;Ⅱ型菌易产孢,后期产生少量菌核;Ⅲ型菌易产孢,且产生大量菌核。产孢分离物均形成直立状分生孢子梗,孢子梗分枝顶端聚生葡萄穗状分生孢子,分生孢子卵圆形或长卵圆形。供试菌株的ITS序列和G3PDH基因序列与灰葡萄孢Botrytis cinerea Pers.的同源性达到99%;致病性测定结果表明,各菌群代表菌株对牡丹均有致病性,但不同菌群间致病力有差异。研究表明,引起洛阳市牡丹灰霉病的病原菌为灰葡萄孢,且菌群类型多样。
In order to clarify the pathogen causing tree peony gray mold, the infected leaves were collected from four tree peony-growing regions in Luoyang in 2015. The pathogenic isolates were obtained by tissue isolation methods and were identified based on morphological characteristics and gene sequencing analysis. The pathogenicity of isolates was carried out in vitro by leaf acupuncture inoculation. The results showed that forty isolates were obtained from infected leaves and they were divided into three groups:group I with sclerotia but without conidia; group Ⅱ with conidia and a little sclerotia; group Ⅲ with conidia and a large number of sclerotia. The conidiophores of spore isolates were erect and the terminal branch expanded with a large number of conidia like grape spikes, the conidia were ovoid or elliptical in shape. The ITS regions and G3PDH gene sequences of the representative isolates were 99% similar to Botrytis cinerea Pers.. The results of tree peony leaves pathogenicity test showed that all the representative isolates could infect the leaves, but their pathogenicity were different and the pathogenic fungus of tree peony gray mold was B. cinerea in Luoyang, and the pathogenic isolate type was complex.
Sequential Analysis of Double Indexes of Common and Variant Peak Ratios in Ultraviolet Fingerprint of Paeonia Suffruticosa Andr. Root Bark
丹皮紫外指纹图谱共有峰率和变异峰率双指标序列分析

Yuan Jiurong,Yue Chunhu,Zou Huabin,Wang Aiwu,
袁久荣
,岳春华,邹华彬,王爱武

世界科学技术-中医药现代化 , 2004,
Abstract: Objective: To analyze the ultraviolet fingerprint of the root bark of Paeonia Suffruticosa Andr. and other medicinal crops of its kind by the method of sequential analysis of the double indexes of common and variant peak ratios in ultraviolet fingerprint. Method: In this article three solvents-Chloroform, absolute ethel alcohol and water are introduced to systematically extract the components of the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in different polar zones, examine their ultraviolet fingerprints and study the differences of the samples. Result: This method is able to accurately identify the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in detail. Conclusion: Tow or several samples can be reliably identified by the method of sequential analysis of the double indexes of common and variaut peak ratios.
AM fungi diversity in the main tree-peony cultivation areas in China
我国洛阳与菏泽牡丹主栽园区AM真菌多样性研究

Shaoxia Guo,Yugang Zhang,Min Li,Runjin Liu,
郭绍霞
,张玉刚,李敏,刘润进

生物多样性 , 2007,
Abstract: In order to characterize arbuscular mycorrhizal(AM) fungal resources in the rhizosphere of tree-peony(Paeonia suffruticosa),we collected soil and root samples,and isolated 35 species in five genera of AM fungi from the main tree-peony cultivation areas,Heze and Luoyang of China. Glomus showed the highest relative abundance,followed by Acaulospora. There were differences in species richness,spore den-sity and species diversity of AM fungi between the two regions. Spore density in the rhizospheric soil of tree-peony grown in Heze Zhaolou Tree-peony Garden was highest,while in soil of the Luoyang Cuoli Tree-peony Nursery it was lowest. The species richness in Heze Zhaolou Tree-peony Garden was lowest. Spe-cies diversity in the rhizospheric soil of tree-peony grown in Heze Tree-peony Nursery was significantly higher than in other sampling sites. Glomus geosporum was the dominant species in Heze Zhaolou Tree-peony Garden and Luoyang Wangcheng Garden,while G. constrictum was the dominant species in Heze Tree-peony Nurs-ery,Heze Zhaolou Tree-peony Garden,Luoyang Tuqiao Seedling Nursery and Luoyang Tree-peony Garden. The results suggest that AM fungal diversity is rich in the main tree-peony cultivation areas.
STEPIC PEONY (PAEONIA TENUIFOLOA L.) A RARE SPECIES IN THE FLORA OF BANAT
L. Schrott,A. Sinitean
Annals of West University of Timi?oara : Series of Biology , 2001,
Abstract: Paeonia tenuifolia L. is a rare species in the Romanian flora, protected in two natural reservations: Zaul de Campie in Transilvania and Fantanita-Murfatlar in Dobrogea. In Banat this species is a remarkable and ancient pontic steppic element and can be found only in one station near Moldovita on mezozoic calcareous grounds. Comparatively with Transilvanian reservation where the steppic peony inhabits in derived mezzo-xerophitic meadows edified by Festuca sulcata and Brachipodium pinnatum, in Banat this species inhabits in communities belonging to Erysimo-Stipetum eriocaulis E. Schneider – Binder et. Al. 1970 and Festucetum sulcatae valesiacae REsm. 1970 associations.
Yinpingmudan, the Wild Relative of the King of Flowers, Paeonia suffruticosa Andrews
银屏牡丹——花王牡丹的野生近亲

HONG De-Yuan,PAN Kai-Yu,XIE Zhong-Wen,
洪德元
,潘开玉,谢中稳

中国科学院研究生院学报 , 1998,
Abstract: Paeonia suffruticosa Andrews subsp. spontanea (Rehder) S. G. Haw et L. A. Lauener ( =P. spontanea = P. jishanensis) has been considered as the wild form of widely cultivated P. suffruticosa. However, the subspecies differs from the cultivated one in shape, division and indumentum of leaves and reproductive biology. As a result of extensive field work, a form with two individuals in Yinping Shan, Caohu, Anhui, and Songxian, Henan, are found to be extremely similar to P. suffruticosa, but apparently different from P. jishanensis. The form is considered to be the wild one of P. suffruticosa and described as new: P. suffruticosa subsp, yinpingmudan Hong, K. Y. Pan et Z. W. Xie.
Herbaceous Peony (Paeonia lactiflora Pall.) as an Alternative Source of Oleanolic and Ursolic Acids  [PDF]
Chunhua Zhou,Ying Zhang,Yanle Sheng,Daqiu Zhao,Sansan Lv,Yue Hu,Jun Tao
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12010655
Abstract: Oleanolic acid (OA) and ursolic acid (UA) have been proven to possess many biological activities, and much attention is focused on the search for plants which are rich in OA and UA. In this report, the OA and UA accumulation characteristics were investigated in 47 cultivars of Chinese herbaceous peony ( Paeonia lactiflora Pall.) and were followed in three cultivars over different developmental stages as measured by high performance liquid chromatography (HPLC). OA and UA levels in leaves and stems demonstrated an overall upward trend from May 1 to September 15 except for UA in the leaves of “Hong Feng”. The maximum values of OA and UA in leaves of “Yangfei Chu Yu”, “Fen Zhu Pan” and “Hong Feng” were 852.98, 575.60, 290.48 μg/g FW and 924.94, 827.36, 432.67 μg/g FW, respectively. The maximum values of OA and UA in stems of “Yangfei Chu Yu”, “Fen Zhu Pan” and “Hong Feng” were 359.28, 90.49, 43.90 μg/g FW and 326.86, 82.25, 56.63 μg/g FW, respectively. OA and UA contents in leaves of 47 different herbaceous peony cultivars ranged from 66.73–618.12 and 36.23–665.14 μg/g FW, respectively, with average values of 171.62 and 227.57 μg/g FW, respectively. The results suggested that the aboveground parts of herbaceous peony may be used as an alternative source of OA and UA for medicinal purposes in addition to its ornamental?purposes.
Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
中国科学院研究生院学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。 在把作丹皮用的栽培牡丹(凤丹)描述为新种P. ostii后,观赏的栽培牡丹仍然被统称为P. suffruticosa。 但在P. suffruticosa复合体内, P. rockii, P. jishanensis, P. qiui 和P. suffruticosa ssp. yinpingmudan 等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P. suffruticosa “是牡丹组数
Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
植物分类学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。在把作丹皮用的栽培牡丹(凤丹)描述为新种P.ostii后,观赏的栽培牡丹仍然被统称为P.suffruticosa。但在P.suffruticosa复合体内,P.rockii,P.jishanensis,P.qiui和P.suffruticosa ssp.yinpingmudan等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P.suffruticosa“是牡丹组数种植物复合体”的说法。本文在详细论述P.suffruticosa复合体分类历史的基础上,根据我们自己对野生类型和栽培类型的考察研究,认为观赏栽培牡丹的形成是多元的,并且包含几个物种;P.suffruticosa有明确的概念,它是观赏栽培牡丹的组成部分,而不代表栽培牡丹的全部,更不是数种植物的复合体。我们认为应根据形态特征正确鉴定观赏栽培牡丹,不应把它们一概鉴定为P.suffruticosa。通过文献和标本引证,文中界定了P.suffruticosa的范围。此外,把P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen作为P.suffruticosa ssp.yinpingmudan D.Y.Hong,K.Y.Pan&Z.W.Xie的异名,并且认定P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen ssp.henanensis(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen是不合法名称。
PRELIMINARY REPORT OF RAPD ANALYSIS IN PAEONIA SUFFRUTICOSA SUBSP.SPONTANEA AND PAEONIA ROCKII
矮牡丹与紫斑牡丹RAPD分析初报

Pel Yan-long Zou Yu-ping Yin Zhen Wang Xiao-quan Zhang Zhi-xian Hong De-yuan,Laboratory of Systematic,Evolutionary Botany,
裴颜龙
,邹喻苹

植物分类学报 , 1995,
Abstract: Ten different oligonucleotide primers of arbitrary sequence were used in Capillary and Air Thermocycler to amplify genomic total DNA of Paeonia suffruticosa subsp. spon-tanea and P. rockii isolated from several local populations in Shanxi, Shaanxi and Gansu provinces. Under the strictly standardized amplification condition for all the primers, these primers yielded clear and reproducible bands corresponding to amplified products and separable by agarose gel electrophoresis. Among a total of 71 bands amplified, 16(22. 5%) were polymorphic in a single individual of P. suffruticosa subsp. spontanea, while among a total of 76 bands 21(27. 6%)were polymophic in a single individual of P. rockii. On an average, the pairwise marker difference between band profiles of conspecific individuals (different populations) was 7. 9 for P. suffruticosa subsp. spontanea and 8. 7 for P. rockii respectively. The average marker difference between P. suffruticosa subsp. spontanea and P. rockii was 10. 3. Obviously, greater number of plants and primers will be required to detect satisfactorily level of genetic diversity. These preliminary results showed that intraspecific genetic diversity was low for the two endangered species. RAPD as a molecular marker was useful and feasible for detecting the genetic variation within species of wild moutans. And it was also potential for studying evolution and relationships between species.
GC-MS analysis of essential oil of Paeonia suffruticosa Andrews from ZhaoFen and RouFurong flowers in China  [PDF]
Weiping Yin, Wenlu Duan, Pu Liu, Ruixue Deng, Yunlai Ren, Shuang Zhao
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.48073
Abstract: The flowers of ZhaoFen and RouFurong may contain essential oils with natural aromatic ingredients. In the present work, the chemical compositions of essential oil of Paeonia suffruticosa Andrews from the flowers of ZhaoFen and RouFurong grown only in China were investigated by GC-MS analysis. The results indicate that there are 27 constituents in ZhaoFen and 29 constituents in RouFurong, which account for 96.04% and 95.90% of the oils of ZhaoFen and RouFurong, respectively. The major components of the essential oils are character-rized by oxygenated terpenols, and their content is, respectively, 85.06% and 83.47%. The essential oil of Paeonia suffruticosa Andrews was reported for the first time on the aerial parts.
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