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Anti-Oxidative Activity of An Aqueous Suspension of Commercial Preparation of The Mushroom Coprinus comatus  [PDF]
Mira Popovi?,Sa?a Vukmirovi?,Neboj?a Stilinovi?,Ivan ?apo,Vida Jakovljevi?
Molecules , 2010, DOI: 10.3390/molecules15074564
Abstract: In this study the effects of an aqueous suspension of a commercial preparation of the mushroom Coprinus comatus on oxidative stress induced in rats by alloxane and carbon tetrachloride was examined. The effects were estimated from changes in the biochemical parameters (xanthine oxidase, glutathione peroxidase and catalase activity, reduced glutathione content, and extent of lipid peroxidation) of liver homogenate as well as histological changes in the liver of the rats treated with alloxane and carbon tetrachloride. Two screening doses of alloxane sufficient to induce diabetes in rats did not have any significant effect on the examined biochemical parameters of liver homogenate or on the cytoarchitectonics of liver cross-sections. Treatment with carbon tetrachloride resulted in a significant increase in the intensity of lipid peroxidation and peroxydasis activity, as well as with decrease in catalase activity. Certain changes in liver cross sections were detected, such is lymphocyte infiltration of dilated sinusoid capillaries. Administration of Coprinus comatus suspension thus showed antioxidative potential, evidenced by an increase of antioxidative status of liver homogenate and prevention of histological changes in liver cross sections.
Antioxidant Properties of Cap and Stipe from Coprinus comatus  [PDF]
Bo Li,Fei Lu,Xiaomin Suo,Haijuan Nan,Bin Li
Molecules , 2010, DOI: 10.3390/molecules15031473
Abstract: Coprinus comatus, also called chicken drumstick mushroom, is currently commercially available in China. Hot water and ethanolic extracts were prepared from cap and stipe of C. comatus fruit bodies and their antioxidant properties were studied. Ethanolic extract from stipe showed high antioxidant activity (80.6%) at 1 mg/mL. Reducing power of hot water extracts from cap was 1.653 at 10 mg/mL. Extracts from cap showed better scavenging ability on DPPH (57.9% at 1 mg/mL) than stipe ones. Ethanolic extracts were more effective in scavenging ability on hydroxyl radicals (57.4–61.3% at 5 mg/mL) than hot water extracts. Ethanolic extracts showed moderate scavenging ability on superoxide radicals (46.3–47.0% at 20 mg/mL). Naturally occurring antioxidant components including total phenols (3.60–20.00 mg/g), tocopherols (0.58–11.93 mg/g), flavonoids (0.19–3.52 mg/g) and polysaccharides (58.52–547.86 mg/g) were found in the extracts. Overall, extracts from cap were more effective for the antioxidant properties assayed.
Some morphological and physiological aspects and cultivation of Coprinus comatus (O. F. Mull.) Gray
Chaiyama, V,Petcharat, V.,Kritsaneepaiboon, P.
Songklanakarin Journal of Science and Technology , 2007,
Abstract: Some morphological and physiological aspects and cultivation of Coprinus comatus (O.F. Mull.) Gray were investigated. Malt extract agar turned out to be the best in supporting the mycelial growth of C.comatus. Mannose and maltose were the best carbon sources in supporting mycelial growth. C. comatus utilized peptone and NH4NO3 better than other nitrogen sources. The optimum temperature and pH on maltextract agar were 25oC and 6, respectively. Light retarded mycelial growth of C. comatus. For cultivation, the method of growing mushroom in autoclavable plastic bags was applied. Three different combinations of agricultural products were used for growing C. comatus. The combination ofpararubber sawdust : kapok waste : boiled sorghum seeds (3:3:1 by volume) supported higher yields of basidiocarps. Time required for full colonization of the mycelia on 500 gm substrate at room temperature(28-30oC) was 20.3 days. After casing with the mixed soil (loam soil : rice husk : cow manure, 2:2:1) the cultivating bags were incubated in the growth chamber at 20oC and 65% relative humidity. Fructificationbegan after 20.6 days of watering, and the average yield obtained was 165.6 g/bag.
Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei

Chunmei Ge,Juanjuan Xu,Qinying Sun,Jie Zhang,Jingmin Cai,Renrui Pan,

生物工程学报 , 2009,
Abstract: In order to enhance the utilization efficiency,reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration;we studied the ligninase production by Coprinus comatus,Aspergillus niger and Trichoderma reesei through the plate screening.The results showed that C.comatus mixed culture with T.reesei have a good compatibility and higher yields of Laccase.On the basis of this pre-experiment,we studied the optimal conditions of mixed culture for enzyme production.Under ...

NI Xin-jiang,FENG Zhi-Yong,LIANG LI-Kun,YOU Cui-Rong,PAN Ying-Jie,

微生物学通报 , 2002,
Abstract: Coprinus comatus cultivated on cotton seed hull medium decomposed lignocellulose straggly and was high of absolute biological efficiency. Lignocellulose is the main carbon source for the fruiting stage of the fungus. There existed the positive correlation between the degradation rates of the cellulose and hemicellulose in the medium and the activities of extracellular CMCase (carboxymenthelcel lulase), FPase (filter paper cellulase) and HCase (hemicellu-lase), there also existed the positive correlation between the degradation rate of the lignin in the medium and the activity of extracellular laccase, but no correlation between the degradatio rate of the lignin in the medium and the activity of peroxidase. The activity of extracellular amylase was comparatively high at mycelial growth stage, and the protease activity peek was at teh time when the fruitbody matured.
毛头鬼伞(Coprinus comatus)中一种碱性蛋白的纯化及其活性
微生物学报 , 2003,
Abstract: 用离子交换层析(CMsepharose FF)和凝胶层析(SuperdexTM75)方法,从新鲜食用菌毛头鬼伞(Coprinus comatus)子实体中分离纯化出一碱性蛋白y3,经SDSPAGE初步确定其分子量约为14.4kD。活性检测结果显示:当其浓度为12.5μg/mL时,对烟草花叶病毒(TMV)在心叶烟枯斑寄主上的侵染抑制率达83.0%;y3对兔血凝集活性滴度为2.5,对人血凝集活性滴度为26,其浓度分别为1.562μg/mL和0.781μg/mL;利用胃癌细胞株MGC803检测y3体外抗肿瘤活性,其IC50为12μg/mL。y3 N端序列为NRDVAACARFIDDFCDTLTP,为一新的蛋白序列。在SWISSPORT上登录号为P83477。
A Comparison of Antinociceptive Activity of Mycelial Extract from Three Species of Fungi of Basidiomycetes
Ganoderma lucidumCoprinus comatusGrifola frondosaGanoderma lucidumppGanoderma lucidumpGanoderma lucidum
The Open Complementary Medicine Journal , 2009, DOI: 10.2174/1876391X00901010073]
Abstract: The antinociceptive activity of mycelial extract from fermented mushroom of Ganoderma lucidum, Coprinus comatus and Grifola frondosa were studied in this paper. The writhing test, formalin test and hot-plate test were used to evaluate the antinociceptive activity of the acetone extracts of the three Species of Fungi of Basidiomycetes in the study respectively. The extract of Ganoderma lucidum inhibited in a dose-dependent manner the acetic acid-induced abdominal constrictions in mice (p<0.05). Also both phases of the response were significantly inhibited in the formalin test (p<0.05). In the hot-plate test, results showed that extract of Ganoderma lucidum significantly inhibited the reaction time to thermal stimuli at 30, 60, and 90 min (p<0.05). The preliminary toxicologic study demonstrated the safety of it. However the extract from fermented mushroom of Coprinus comatus and Grifola frondosa did not show significantly expectative antinociceptive effect. Our results show the acetone extract of Ganoderma lucidum is a potent analgesic drug.

Jennifer MFWAN,Daniel KOCHAN,

菌物学报 , 2003,
Abstract: glucan is a core structure in mushroom polysaccharide, which claim to posses anticancerous and immunomodulatory properties. The ability to identify (1, 3)--glucan in mushroom polysaccharide not only provide useful information on the structural composition of the mushroom polysaccharide, but facilitate us to identify the potential anticancerous and immunomodulatory active compounds. Using limulus factor G test, we detected (1, 3)--glucan in 27 polysaccharides extracted from 19 edible or medicinal mushrooms species. The result show that (1, 3)--glucan exists in all mushroom polysaccharide extracts but its content varies extremely depends on the mushroom species or the part of mushroom and the degree of purification. Our data show the mean of (1, 3)--glucan in these mushroom polysaccharides is 34.8%. Nine mushroom polysaccharide extracts from Lentinus edodes, Schizophyllum commune, Coriolus versicolor, Volvariella volvacea, Coprinus comatus, Grifola frondosa, Lyophyllum shimeji have superior (1, 3)--glucan contents than the others. Our study demonstrates that limulus Factor G test is a quick and convenient method for detecting (1, 3)--glucan content in crude mushroom polysaccharide extracts.
Analysis of genetic diversity among germplasm resource of 57 strains of Coprinus comatus

JIANG Yu-Ji,XIE Bao-Gui,DENG You-Jin,LIU Xin-Rui,JIANG Hong,

菌物学报 , 2013,
Abstract: 运用SRAP、RAPD、ISSR 3种分子标记技术对来源不同地区的57株毛头鬼伞Coprinus comatus进行了遗传多样性分析,通过3种分子标记进行聚类分析,当相异系数D为0.48时,可以把57株毛头鬼伞分为4类:Ⅰ类包括Co0001;Ⅱ类包括Co0003;Ⅲ类包括Co0005;Ⅳ类包括其余54个菌种。供试的57个菌株间的相异系数范围从0–0.72,具有一定的遗传多态性。但其中有许多菌株两者之间的相异系数为0,说明毛头鬼伞菌种存在着比较严重的同种异名现象。
Fatty Acid Compositions of Five Wild Edible Mushroom Species Collected from Turkey  [PDF]
Pelin Gunc Ergonul,Bulent Ergonul,Fatih Kalyoncu,Ilgaz Akata
International Journal of Pharmacology , 2012,
Abstract: The fatty acids of five wild edible mushroom species (Agrocybe cylindracea, Coprinus comatus, Lactarius deliciosus, Suillus collinitus and Tricholoma myomyces) collected from different regions from Anatolia were determined. The fatty acids were identified and quantified by gas chromatography and studied using fruit bodies. Fatty acid composition varied among species. The dominant fatty acid in fruit bodies of all mushrooms was cis-linoleic acid (18: 2). Percentage of cis-linoleic acid in species varied from 36.29-66.72%. The other major fatty acids were, cis-oleic, palmitic and stearic acids, respectively. Fatty acids analysis of the mushrooms showed that the unsaturated fatty acids were at higher concentrations than saturated fatty acids.
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