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Expression of Human Lactoferrin in Transgenic Potato Tubers

SONG Dong-guang,CHEN Xiao-ying,ZHANG Hui-song,LI Jun,ZHANG Xiao-feng,WANG Hui-zhen,

热带亚热带植物学报 , 2008,
Abstract: Human lactoferrin (hLF) is a member of non-specific immune system, it plays many roles, such as resistances against bacteria, fungi and viruses, etc. In this paper, hLF cDNA directed by the tuber specific promoter of class I patatin gene of potato (Solanum tuberosum L.) were transferred into potato mediated by Agrobacterium tumefiens transformation. hLF transgenic potato plants were confirmed by PCR detection, further RT-PCR showed that hLF RNAs were expressed in transgenic plants. hLF activities were detected by ELISA and Western blot analysis in hLF transgenic potato tubers.
Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans  [PDF]
Jong-Kook Lee,Ramamourthy Gopal,Chang Ho Seo,Hyeonsook Cheong,Yoonkyung Park
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13044021
Abstract: In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato ( Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys 3 and Cys 9 or Cys 10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.
BUITRAGO R.,Diana M.; RAMOS R.,Gloria; RINCóN V.,Javier; GUERRERO P.,Mario F.;
Vitae , 2007,
Abstract: the plant solanum tuberosum is used in colombian folk medicine for the treatment of hypertension. we evaluated the antiaggregant properties on human platelets of an ethanolic extract (96%) obtained from the skin of tubers of this species. extracts from two varieties, sabanera and pastusa revealed in vitro dose-dependent (10-100 mg/ml) antiaggregant effects on platelets stimulated with adenosindiphosphate (adp 10 μm), epinephrine (300 μm), collagen (10 μg/ml), and arachidonic acid (150 μg/ml). the ic50 values for the sabanera and pastusa varieties were, respectively, 1,8 μg/ml and 0,4 mg/ml, against adp; 4,4 μg/ml and 0,1 mg/ml against epinephrine; 34,1 μg/ml and 1,3 mg/ml against collagen, and 12,3 μg/ml and < 0,1 μg/ml, against arachidonic acid, a key precursor in prostaglandin synthesis. our findings suggest that s. tuberosum has a promising pharmacological profile since it combines the hypotensive effects, previously described, with the antiaggregant ones; therefore, it is potentially useful for the treatment of cardiovascular disorders such as hypertension and coronary artery disease.
Activity of alternative oxidase and plant uncoupling mitochondrial protein in potato tubers stored at low temperature or submitted to artificial aging
Pinheiro, Hugo Alves;Borges, Regis;Silva, Marco Aurélio Pedron e;Centeno, Danilo Cruz;
Brazilian Journal of Plant Physiology , 2004, DOI: 10.1590/S1677-04202004000200001
Abstract: potato tubers (solanum tuberosum l. cv. binje) were stored at 18 and 4oc in order to verify changes in mitochondrial metabolism, especially in the activities of the alternative oxidase (aox) and plant uncoupling mitochondria protein (pump). tubers were also sliced and submitted to artificial aging. the oxygen consumption rate (ocr), respiratory control (rc) and adp/o ratio for mitochondria obtained after storage at 18oc were not altered, regardless of the time of storage. considerable increment in ocr, for both respiratory states 3 and 4, was observed after storing the tubers for five and ten days at 4oc; and it was accomplished by reductions in rc and adp/o. the aox activity was evident after five days at 4oc, and it was enhanced after ten days of storage at that temperature. no significant changes in pump activities were observed after one day at 18oc or at 4oc, neither after artificial aging. however, increased swelling of potato mitochondria was observed in the presence of valinomycin and linoleic acid when the tubers were kept at 4oc for five and ten days. besides the changes in aox and pump activities, slight alterations in ascorbate peroxidase (apx) and catalase (cat) activities were observed after storing the tubers at 4oc for 5 days. the activities of these enzymes were higher after storage at 4oc for 10 days. the results suggest the combined effect of low temperature and time of exposure in promoting aox activity, probably as a protective mechanism against cell damage in response to mitochondrial oxidative stress.
Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L.) tubers  [PDF]
Mohammed Bajji,Mahmoud M’Hamdi,Frédéric Gastiny,Jorge A. Rojas-Beltran
Biotechnologie, Agronomie, Société et Environnement , 2007,
Abstract: The involvement of hydrogen peroxide (H2O2) metabolism in dormancy release and sprouting of potato (Solanum tuberosum L.) tubers has been investigated using three complementary approaches. In the first approach, the evolution of the sprouting kinetics, H2O2 content and antioxidant enzyme activities were examined during tuber storage. The most important changes occurred at the bud/sprout level. In particular, dormancy release was accompanied by a transient but remarkable increase in H2O2 content. In the second approach, the effect of a catalase (CAT, EC inhibitor (thiourea) or of exogenous H2O2 application on tuber sprouting behaviour was assessed. Both treatments resulted in a reduction of the dormancy period and in rapid and synchronised sprouting of the treated tubers when compared to the control as well as in increased sprout number per tuber. In the third approach, the effect of CAT inhibition on potato tuber dormancy and sprouting was evaluated using the transgenic technology. Plants partially repressed in their CAT activity were produced and, once again, CAT inhibition resulted in acceleration of the sprouting kinetics and in increased sprout number of the transgenic tubers compared to those from the wild type. It thus appears that tuber dormancy and sprouting can be controlled in potato by the manipulation of H2O2 metabolism via the inhibition of CAT activity. The possible mechanisms whereby CAT inhibitors or H2O2 overcome dormancy and promote sprouting in the potato tuber are discussed in relation to what is known in other plant models (seeds and fruit tree buds).
Diana M. BUITRAGO R.,Gloria RAMOS R.,Javier RINCóN V.,Mario F. GUERRERO P.
Vitae , 2007,
Abstract: Se evaluó la actividad antiagregante en plaquetas humanas del extracto etanólico (96%, residuo seco) obtenido de las cáscaras de los tubérculos de Solanum tuberosum, especie medicinal que se utiliza etnobotánicamente en Colombia para el tratamiento de la hipertensión arterial. El material obtenido de dos variedades, sabanera y pastusa, presentó, en función de la dosis (1 μg/mL -100 μg/mL), efectos antiagregantes in vitro en plaquetas estimuladas con adenosindifosfato (ADP 10 μM), epinefrina (300 μM), colágeno (10 μg/mL) y ácido araquidónico (AA 150 μg/mL). Los valores de CI50 obtenidos por las variedades sabanera y pastusa fueron respectivamente de: 1,8 μg/mL y 0,4 mg/mL frente a ADP, 4,4 μg/mL y 0,1 mg/mL frente a epinefrina, 34,1 μg/mL y 1,3 mg/mL, frente a colágeno y 12,3 μg/mL y < 0,1 μg/mL frente al ácido araquidónico. El perfil antiagregante dosis-respuesta de la variedad pastusa fue similar al descrito por el ácido acetil salicílico (ASA) frente a AA, precursor clave en la síntesis de prostaglandinas. Los hallazgos de este estudio sugieren que S. tuberosum posee un perfil farmacológico promisorio, dado que conjuga efectos hipotensores, descritos con anterioridad, con efectos antiagregantes, favoreciendo así su potencial terapéutico en el tratamiento de trastornos cardiovasculares tales como hipertensión arterial y enfermedad coronaria. The plant Solanum tuberosum is used in Colombian folk medicine for the treatment of hypertension. We evaluated the antiaggregant properties on human platelets of an ethanolic extract (96%) obtained from the skin of tubers of this species. Extracts from two varieties, sabanera and pastusa revealed in vitro dose-dependent (10-100 mg/mL) antiaggregant effects on platelets stimulated with adenosindiphosphate (ADP 10 μM), epinephrine (300 μM), collagen (10 μg/mL), and arachidonic acid (150 μg/mL). The IC50 values for the sabanera and pastusa varieties were, respectively, 1,8 μg/mL and 0,4 mg/mL, against ADP; 4,4 μg/mL and 0,1 mg/mL against epinephrine; 34,1 μg/mL and 1,3 mg/mL against collagen, and 12,3 μg/mL and < 0,1 μg/mL, against arachidonic acid, a key precursor in prostaglandin synthesis. Our findings suggest that S. tuberosum has a promising pharmacological profile since it combines the hypotensive effects, previously described, with the antiaggregant ones; therefore, it is potentially useful for the treatment of cardiovascular disorders such as hypertension and coronary artery disease.
Evaluation de l'efficacité de la Bacillus thuringiensis contre les larves et imagos de Andrector ruficornis sur les plants de Solanum tuberosum au Cameroun
Ambang, Z.,Omokolo, ND.,Ouzounov, IS.
Tropicultura , 2002,
Abstract: Evaluation of the Efficiency of Bacillus thuringiensis on Larvae and Adults of Andrector ruficornis on Solanum tuberosum plants in Cameroon. The efficiency of Bacillus thuringiensis as an insecticide on the larvae and adults of A. ruficornis (a leaf eating pest) was investigated on Solanum tuberosum plants grown in the field. The results show that, one hour following the treatment of A. ruficornis with a 1.4 g/l suspension of Bacillus thuringiensis, larvae and adults are paralysed on the plants. A high mortality of the insects is observed 48 hours later and the mortality rate reaches 100% after 72 hours. The use of bitoxibacilline (made of Bacillus thuringiensis spores) results in an increase in tuber production (32%) as well as the starch of tubers (from 18 to 27% of fresh weight) and vitamin C (from 9.7 to 11.9 mg/100g fw of tubers). Our observations show that Bacillus thuringiensis powder is an efficient insecticide specific to potato Chrysomelidae.
Loyola L,Nelson; Oyarce C,Eduardo; Acu?a C,Carlos;
Idesia (Arica) , 2010, DOI: 10.4067/S0718-34292010000200005
Abstract: chemical composition of potato tubers (solanum tuberosum, sp. tuberosum) cv. desirée was evaluated after two production alternatives; conventional and organic, and the components were: dry matter content, starch, proteins, total and reducing sugars. a total randomized design was used and the values were measured by the anova with a 95% of probability. there were no significant differences among the parameters evaluated under the two cultivation alternatives. sensory evaluation with fried potato were also realized by using tubers from the two cultivation alternatives, conventional and organic, at 30 days and 75 days after harvest. thirteen trained panelist participated, who evaluated the following attributes; color, texture and flavor. according to the panelist there were no differences in relation to any kind of potato tubers, after fried, whatever the cultivation alternatives, in relation to the sensory attributes.
Detection of latent infection by Ralstonia solanacearum in potato (Solanum tuberosum) using stems instead of tubers
JK Mwangi, AB Nyende, P Demo, VN Matiru
African Journal of Biotechnology , 2008,
Abstract: The potential of using stems for the detection of latent infection caused by Ralstonia solanacearum (Rs) was studied. Forty plants each were collected from four farms with bacterial wilt incidence below 4% in two growing seasons (season A and season B of 2005). The tubers of all the selected plants including 10 cm of the all lower stems were collected. Samples were taken to the laboratory for indexing against R. solanacearu (Rs) using ELISA techniques. The Rs status of each of the composite samples of all the tubers and of stems was determined and then correlation coefficients computed. There was a notable difference in the percentage number of samples per farm with particular categories of R. solanacearum status. When stems were compared to tubers for detection of Rs, an average r – value of 0.4 was obtained when r-values for the four different farms were averaged. The lowest r-value recorded was 0.2 while the highest was 0.5. When individual farms were considered it was only in one farm out of the four that r was not significant (p = 0.2). Overall the r-value was significant (p < 0.05). These results indicate that there is scope for adoption of stems as an alternative sample to tubers for indexing against R. solanacearum in potato tuber seed certification schemes more so in screening for presence of R. solanacearum in seed potato fields. However, although significant, the low r-value calls for more investigations to be done prior to final recommendation on use of stems from potato fields.
Powdery scab effect on the potato Solanum tuberosum ssp. andigena growth and yield
Gilchrist, Elizabeth;Soler, Juliana;Merz, Ueli;Reynaldi, Sebastian;
Tropical Plant Pathology , 2011, DOI: 10.1590/S1982-56762011000600002
Abstract: powdery scab affects most potato production zones in the world. the causal organism spongospora subterranea f. sp. subterranea forms galls in roots and lesions on tubers during its replication. recent researches suggest that s. subterranea could cause harmful effects on the host plant and potentially on yield.in order to quantify the disease impact on plant growth and yield, potato solanum tuberosum ssp. andigena, cv. diacol capiro was grown in a soil infested with s. subterranea and compared to a healthy control soil. data was taken at the flowering stage and at harvest. s. subterranea caused a plant length reduction of 23%, a foliar dry weight reduction of 32% and a tuber weight reduction of 30%, whereas s. subterranea did not reduce the number of tubers or stems.
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