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Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues  [cached]
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2010,
Abstract: An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.
Green turtles (Chelonia mydas) foraging at Arvoredo Island in Southern Brazil: genetic characterization and mixed stock analysis through mtDNA control region haplotypes
Proietti, Maíra Carneiro;Lara-Ruiz, Paula;Reisser, Júlia Wiener;Pinto, Luciano da Silva;Dellagostin, Odir Antonio;Marins, Luis Fernando;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009000300027
Abstract: we analyzed mtdna control region sequences of green turtles (chelonia mydas) from arvoredo island, a foraging ground in southern brazil, and identified eight haplotypes. of these, cm-a8 (64%) and cm-a5 (22%) were dominant, the remainder presenting low frequencies (< 5%). haplotype (h) and nucleotide ( π) diversities were 0.5570 ± 0.0697 and 0.0021 ± 0.0016, respectively. exact tests of differentiation and amova φst pairwise values between the study area and eight other atlantic foraging grounds revealed significant differences in most areas, except ubatuba and rocas/noronha, in brazil (p > 0.05). mixed stock analysis, incorporating eleven atlantic and one mediterranean rookery as possible sources of individuals, indicated ascension and aves islands as the main contributing stocks to the arvoredo aggregation (68.01% and 22.96%, respectively). these results demonstrate the extensive relationships between arvoredo island and other atlantic foraging and breeding areas. such an understanding provides a framework for establishing adequate management and conservation strategies for this endangered species.
OBSERVATION ON THE DEVELOPMENT OF BLOOD CELLS OF CHELONIA MYDAS
绿海龟(Chelonia mydas)血细胞发育过程的观察

LI Chang-Ling,CAO Fu-Jun,HUANG Xiang-Hu,LIU Chu-Wu,HUANG Qing-Jia,
李长玲
,曹伏君,黄翔鹄,刘楚吾,黄庆嘉

海洋与湖沼 , 2009,
Abstract: The blood cells of Chelonia mydas was studied through observation on stained imprint and smear of the peripheral blood, the bone marrow, the spleen and the liver etc. The stain method used is Wright's. The results show that the bone marrow and spleen were the main hemopoietic organs in C. mydas. Erythrocytes, monocyte and granulocytes developed mainly in the bone marrow; and lymphocytes developed mainly in the spleen. The spleen with late immature erythrocytes could be the place of maturation of erythrocytes. No primitive blood cells were observed in liver, which implies that the liver was not the hemopoietic organ in C. mydas. The development of erythrocytes includes 5 stages: primitive, early, middle, late, and mature. The body sizes of these cells increased first, then decreased, and increased in the end. The development of granulocytes includes 5 stages also: myeloblast, promyelocyte, meddle immature granulocytes, late immature granulocytes, and mature granulocytes. The body sizes of them decreased; and the sublobe of the nuclear may indicate the aging. All the four types of blood cells changed from with-nucleolus to without-nucleolus. Mitosis may take place in the primitive blood cells and immature ones, and direct division may occur in some mature red blood cells in the peripheral blood system.
Morfologia do tubo digestório da tartaruga verde (Chelonia mydas)
Magalh?es, Marcela S.;Freitas, Maria de Lourdes;Silva, Naisandra B. da;Moura, Carlos Eduardo B. de;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000800012
Abstract: the morphology of the digestive tube of the green turtle (chelonia mydas) was evaluated. nine young e 1 adult turtles were analyzed. the digestive organs withdrew occurred after plastron opening. the length's description and measurement from each organ [esophagus, stomach, small intestine (si) and large intestine (li)] were made with digestive tube opened. the results showed that in both the adult and young animals, the esophagus from all species were marked by the presence of pointed papillae on internal mucous. the stomach presented saclike shape with blind fund. in the si, reticular pleats in the duodenal mucous membrane have been observed, while the jejune and the ileum showed rectilinear longitudinal pleats. the li was marked by the alternation of arched areas (haustra or sacculations) and narrowings. the microscopy of digestive tube from c. mydas revealed esophagus with folded mucous covered by keratinized and stratified squamous epitheliums. the stomach was divided in regions: cardiac, fundic e pyloric which differed as to the number of glands and the muscle layer's arrangement. the si presented marked by microvillus, and li by folded mucous with glands the lamina propria. the morphology of the tube digestive of the green turtle showed to be adapted to your eating habits. a long the digestive tract and specializations provide greater area of absorption and more efficiency in digestion in this species.
Detecting patterns of fertilization and frequency of multiple paternity in Chelonia mydas of Colola (Michoacán, Mexico)
Lara-De La Cruz, Libny Ingrid;Oyama Nakagawa, Ken;Cano-Camacho, Horacio;Zavala-Paramo, María Guadalupe;Vázquez-Marrufo, Gerardo;Chassin-Noria, Omar;
Hidrobiológica , 2010,
Abstract: we present a microsatellite analysis that allows an indirect determination of the process of fertilization in the genital tract of chelonia mydas. the strategy was based in that the order of oviposition is related to fertilization order. once the genotype of the offspring was obtained through microsatellite analyses, it was possible to determine the frequency of multiple paternity and to infer the presence of anatomical structures or physiological mechanisms that allow females to undergo cryptic choice processes, which allowed us to hypothesize the existence of post-copula factors that determine reproductive success in a polyandrous system. this paper does not show the presence of physiological mechanisms that allow control of fertilization order in polyandrous females of chelonia mydas.
Blood Gases, Biochemistry, and Hematology of Galapagos Green Turtles (Chelonia Mydas)  [PDF]
Gregory A. Lewbart, Maximilian Hirschfeld, Judith Denkinger, Karla Vasco, Nataly Guevara, Juan García, Juanpablo Mu?oz, Kenneth J. Lohmann
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096487
Abstract: The green turtle, Chelonia mydas, is an endangered marine chelonian with a circum-global distribution. Reference blood parameter intervals have been published for some chelonian species, but baseline hematology, biochemical, and blood gas values are lacking from the Galapagos sea turtles. Analyses were done on blood samples drawn from 28 green turtles captured in two foraging locations on San Cristóbal Island (14 from each site). Of these turtles, 20 were immature and of unknown sex; the other eight were males (five mature, three immature). A portable blood analyzer (iSTAT) was used to obtain near immediate field results for pH, lactate, pO2, pCO2, HCO3?, Hct, Hb, Na, K, iCa, and Glu. Parameter values affected by temperature were corrected in two ways: (1) with standard formulas; and (2) with auto-corrections made by the iSTAT. The two methods yielded clinically equivalent results. Standard laboratory hematology techniques were employed for the red and white blood cell counts and the hematocrit determination, which was also compared to the hematocrit values generated by the iSTAT. Of all blood analytes, only lactate concentrations were positively correlated with body size. All other values showed no significant difference between the two sample locations nor were they correlated with body size or internal temperature. For hematocrit count, the iSTAT blood analyzer yielded results indistinguishable from those obtained with high-speed centrifugation. The values reported in this study provide baseline data that may be useful in comparisons among populations and in detecting changes in health status among Galapagos sea turtles. The findings might also be helpful in future efforts to demonstrate associations between specific biochemical parameters and disease.
Learedius learedi Price 1934 (Digenea, Spirorchiidae) in Chelonia mydas Linnaeus 1758 (Testudines, Chelonidae) in Brazil: case report
Werneck, M.R.;Becker, J.H.;Gallo, B.G.;Silva, R.J.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2006, DOI: 10.1590/S0102-09352006000400016
Abstract: this study reports the occurrence of learedius learedi price 1934 (digenea, spirorchiidae) in chelonia mydas linnaeus 1758 (testudines, chelonidae) in brazil. eleven animals were included in this study, 54.6 % of them were parasitized. two hundred and fifty five parasite specimens were recovered from heart, liver, spleen, lungs, kidneys, mesenterium, and body wash. results contribute to the knowledge about the helminthofauna of marine chelonian and their geographical distribution. this is the first report of l. learedi in the southwestern atlantic.
Blood Gasses Contents of Green Turtle (Chelonia mydas) Hatch Treated by Different Temperatures  [cached]
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2011,
Abstract: The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used to support for turtle hatchery in Indonesia.
Concentration of heavy metals in tissues of green turtles (Chelonia mydas) sampled in the Cananéia estuary, Brazil
Barbieri, Edison;
Brazilian Journal of Oceanography , 2009, DOI: 10.1590/S1679-87592009000300007
Abstract: thirty specimens (15 adults and 15 juveniles) of chelonia mydas found in the cananéia estuary in the state of s?o paulo on the southeastern brazilian coast between january 2005 and september 2006, were analyzed the concentrations of cd, cu, pb, mn and ni in liver and kidney samples of adult and juvenile green turtles were determined by flame atomic absorption spectrophotometry.the average cd concentration found in adult livers (0.57μg.g-1) was significantly higher than that in juveniles (0.279μg.g-1). cu concentrations were significantly higher in the liver than in the kidney, and significantly higher in adults (39.9μg.g-1) than in juveniles (20.7μg.g-1) average mn concentrations in liver and kidney did not differ between adults (4.32 and 4.17μg.g-1) and juveniles (4.81 and 3.82μg.g-1), whereas ni concentrations in adults (0.28 and 0.19μg.g-1, respectively) were significantly higher than in juveniles (0.13 and 0.089μg.g-1, respectively). pb concentrations in liver were significantly higher in adults (0.37μg.g-1) than in juveniles (0.06μg.g-1). the concentrations of essential trace elements in chelonia mydas were generally comparable to values reported in other, similar studies. with respect to non-essential metals (cd, pb and ni), chelonia mydas presented lower values than those reported for their northern atlantic counterparts.
Flow cytometry as a tool in the evaluation of blood leukocyte function in Chelonia mydas (Linnaeus, 1758) (Testudines, Cheloniidae)
Rossi, S.;Sá-Rocha, VM.;Kinoshita, D.;Genoy-Puerto, A.;Zwarg, T.;Werneck, MR.;Sá-Rocha, LC.;Matushima, ER.;
Brazilian Journal of Biology , 2009, DOI: 10.1590/S1519-69842009000400019
Abstract: chelonia mydas is a sea turtle that feeds and nests on the brazilian coast and a disease called fibropapillomatosis is a threat to this species. because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. in order to achieve this aim, blood samples were collected from c. mydas with or without fibropapillomas captured on the s?o paulo north coast. blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile rpmi 1640 cell culture medium. leukocytes were separated by density gradient using ficoll-paquetm plus, amershan biociences?. the following stimuli were applied in the assessment of leukocyte function: phorbol miristate-acetate (pma) for oxidative burst activity evaluation and zymosan a (saccharomyces cerevisiae) bio particles?, alexa fluor? 594 conjugate for phagocytosis evaluation. three cell populations were identified: heterophils, monocytes and lymphocytes. monocytes were the cells responsible for phagocytosis and oxidative burst.
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