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Multivariate analysis of combining ability for soybean resistance to Cercospora sojina Hara
Gravina, Geraldo de Amaral;Sediyama, Carlos Sigueyuki;Martins Filho, Sebasti?o;Moreira, Maurílio Alves;Barros, Everaldo Gon?alves de;Cruz, Cosme Dami?o;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000300015
Abstract: seven soybean cultivars (bossier, cristalina, davis, kent, lincoln, paraná and uberaba), with different levels of resistance to cercospora sojina, race 04, were crossed according to a diallel design, with no reciprocals, to determine the general and the specific combining abilities for the resistance. the evaluations of the reaction to the disease were performed 20 days after the inoculation of the fungus on the most infected leaflet of the plant, in the parents and in the f1 hybrids. to quantify the resistance, the following characteristics were evaluated: infection degree (id); number of lesions per leaflet (nll); lesion mean diameter (lmd); lesioned leaf area (lla); percentage of lesioned leaf area (plla); number of lesions per square centimeter (nlc) and disease index (di). the relative importance of each characteristic was evaluated by the canonical variables analysis and the lla and nll characteristics were eliminated from the multivariate function. with the remaining five characteristics, a multivariate index was created using the first canonical vector, which was submitted to the diallel analysis, according to griffings fixed model, method 2. the most important characters to discriminate resistant from susceptible soybean plants to c. sojina were: id, lmd, nlc, di and plla. cristalina, davis and uberaba cultivars are the best ones among those tested that can be recommended as parents in soybean breeding programs seeking resistance to cercospora sojina. the additive, dominant and epistatic genetic effects were important for the expression of the resistance, although the additive genetic effect was the most important component.
Inheritance of resistance to race 7 of Cercospora sojina in soybeans and RAPD tagging of the resistance gene

科学通报(英文版) , 1999,
Abstract: Frog-eye leaf spot of soybean caused by Cercospora sojina Hara is a kind of worldwide disease. Resistance to race 7 of C. sojina was found to be due to a single dominant gene through analyzing resistant behavior of the cross NEAU91212 (susceptible to race 7) × NEAU9674 (resistant to all races). 3 RAPD markers linked to the resistant gene Rcsc 7 were identified using the BSA method. 2 fragments of OPS03620 and OPS03580 amplified with primer OPS03 co-dominantly segregated in the F2 individuals. The genetic distance between OPS03620 and the resistant gene is 8.7 cM. According to the co-dominant marker, the accuracy of predicting the homozygous and heterozygous resistant F2 individuals were 100% and 97.6% , respectively.
Parametros genéticos da resistência da soja a Cercospora sojina
Gravina, Geraldo de Amaral;Martins Filho, Sebasti?o;Sediyama, Carlos Sigueyuki;Cruz, Cosme Dami?o;
Pesquisa Agropecuária Brasileira , 2004, DOI: 10.1590/S0100-204X2004000700006
Abstract: the inheritance of soybean resistance to cercospora sojina hara was studied based on genetic parameters estimated by the analysis of means and variances of a multivariate index. the crosses between two resistant cultivars, paraná (p) and uberaba (u), with a susceptible, bossier (b), were used. the parents and f1, f2, bcr and bcs generations from each cross were evaluated on five characteristics associated to the disease: visually evaluated infection degree; lesion mean diameter; percentage of lesioned leaf area; number of lesions per square centimeter; and disease index. a multivariate index previously established was applied to the data of the generation analyses. the additive genetic effect was the most important in the determination of the characters related to the resistance of soybean to c. sojina. on both crosses (pxb and uxb) at least one kind of epistatic interaction (aa, ad and dd) was significant and the additive-dominant-epistatic model was more adequate to describe the resistance of soybean to c. sojina.
Diallel analysis for frogeye leaf spot resistance in soybean
Gravina, Geraldo de Amaral;Sediyama, Carlos Sigueyuki;Martins Filho, Sebasti?o;Moreira, Maurílio Alves;Barros, Everaldo Gon?alves de;
Pesquisa Agropecuária Brasileira , 2003, DOI: 10.1590/S0100-204X2003000600002
Abstract: seven soybean cultivars (bossier, cristalina, davis, kent, lincoln, paraná and uberaba), with different levels of resistance to cercospora sojina, were crossed in a diallel design to determine the general (gca) and specific (sca) combining abilities relative to the inheritance of the resistance. race 04 of the fungus was inoculated in the parents and in the 21 f1 hybrids in a greenhouse in a completely randomized design, with 12 replications. the reactions to the disease were evaluated 20 days after the inoculation, always on the most infected leaflet. both gca and sca were significant for all the evaluated characters, being inferred that, for the expression of the characters, the additive, dominant and, possibly, epistatic genic actions were important. the largest values of estimated sca effect (ij) were observed in the hybrid combinations where at least one parent presented high gca. cristalina, davis and uberaba cultivars showed the largest estimates for gca effect (i), and from the analysis of ii, the contribution of these parents to heterosis of their hybrids will be towards the reduction of the disease symptoms. therefore, these cultivars are indicated as parents in breeding programs that seek the development of soybean cultivars with resistance to frogeye leaf spot.
Diallel analysis for frogeye leaf spot resistance in soybean  [cached]
Gravina Geraldo de Amaral,Sediyama Carlos Sigueyuki,Martins Filho Sebasti?o,Moreira Maurílio Alves
Pesquisa Agropecuária Brasileira , 2003,
Abstract: Seven soybean cultivars (Bossier, Cristalina, Davis, Kent, Lincoln, Paraná and Uberaba), with different levels of resistance to Cercospora sojina, were crossed in a diallel design to determine the general (GCA) and specific (SCA) combining abilities relative to the inheritance of the resistance. Race 04 of the fungus was inoculated in the parents and in the 21 F1 hybrids in a greenhouse in a completely randomized design, with 12 replications. The reactions to the disease were evaluated 20 days after the inoculation, always on the most infected leaflet. Both GCA and SCA were significant for all the evaluated characters, being inferred that, for the expression of the characters, the additive, dominant and, possibly, epistatic genic actions were important. The largest values of estimated SCA effect (ij) were observed in the hybrid combinations where at least one parent presented high GCA. Cristalina, Davis and Uberaba cultivars showed the largest estimates for GCA effect (i), and from the analysis of ii, the contribution of these parents to heterosis of their hybrids will be towards the reduction of the disease symptoms. Therefore, these cultivars are indicated as parents in breeding programs that seek the development of soybean cultivars with resistance to frogeye leaf spot.
Triple Test Cross in Genetic Analysis of Soybean Resistance to Cercospora sojina Hara
大豆灰斑病抗性遗传的三点测交分析 Triple Test Cross in Genetic Analysis of Soybean Resistance toCercospora sojina Hara

Cao Yueping,Yang Qingkai,
曹越平
,杨庆凯CAO Yue-Ping,YANG Qing-Kai

遗传 , 1996,
Abstract: ?In this paper, Triple Test Cross Design was used in studing the resistance of soybean to 10 phys-iological race of Cercospora Sojina Haraby inoculation. Results of analysis of gene effects of resistanceindicated that additive effect is significant in all the three crosses, dominant effcct exsists only in the cross 1and epistatic effect remains in the cross 2 and cross 3.
Resistance to stem canker, frogeye leaf spot and powdery mildew of soybean lines lacking lipoxigenases in the seeds
Martins, Carlos Alberto Osório;Sediyama, Carlos Sigueyuki;Oliveira, Maria Goreti de Almeida;Reis, Múcio Silva;Rocha, Valterley Soares;Moreira, Maurílio Alves;Gomes, José Luiz Lopes;
Scientia Agricola , 2002, DOI: 10.1590/S0103-90162002000400013
Abstract: the soybean [glycine max (l.) merrill] crop holds a prominent position in the brazilian economy because of the extension of the planted area and volume of grain production, but the beany flavor has been a limiting factor for soybean derivatives consumption by western population. this flavor is produced mainly by action of lipoxygenase enzymes (lox1, lox2 and lox3), present in some commercial varieties. the genetic elimination of the alleles that codify these enzymes is the most appropriate way to avoid problems associated to this deleterious flavor. to elucidate the effect of seed lipoxygenase elimination on the resistance to plant pathogens, normal varieties of soybean (ft-cristalina rch, doko rc and iac-12) and their backcross-derived lines, both with the three lipoxygenases present in their seeds (triple-positive, tp) and without the three lipoxygenases (triple-null, tn), were tested for their resistance to stem canker (diaporthe phaseolorum f.sp. meridionalis), frogeye leaf spot (cercospora sojina hara), and powdery mildew (microsphaera diffusa cke. & pk.). all genetic materials studied were resistant to stem canker. ft-cristalina rch and doko-rc and their tp and tn lines were resistant to frogeye leaf spot. iac-12 and its derived lines not only presented a higher disease index, but also the derived lines, tp and tn, were more susceptible, indicating the loss of genes for disease resistance in the backcrosses. there was no association between the elimination of lipoxygenases from the seeds with the resistance to frogeye leaf spot. in relation to the powdery mildew, tp or tn lines presented similar or higher resistance than their respective recurrent parents whose susceptibility appeared in the following order: iac-12, less susceptible, doko-rc, intermediate and ft-cristalina rch, more susceptible.
Resistance to stem canker, frogeye leaf spot and powdery mildew of soybean lines lacking lipoxigenases in the seeds
Martins Carlos Alberto Osório,Sediyama Carlos Sigueyuki,Oliveira Maria Goreti de Almeida,Reis Múcio Silva
Scientia Agricola , 2002,
Abstract: The soybean [Glycine max (L.) Merrill] crop holds a prominent position in the Brazilian economy because of the extension of the planted area and volume of grain production, but the beany flavor has been a limiting factor for soybean derivatives consumption by western population. This flavor is produced mainly by action of lipoxygenase enzymes (Lox1, Lox2 and Lox3), present in some commercial varieties. The genetic elimination of the alleles that codify these enzymes is the most appropriate way to avoid problems associated to this deleterious flavor. To elucidate the effect of seed lipoxygenase elimination on the resistance to plant pathogens, normal varieties of soybean (FT-Cristalina RCH, Doko RC and IAC-12) and their backcross-derived lines, both with the three lipoxygenases present in their seeds (triple-positive, TP) and without the three lipoxygenases (triple-null, TN), were tested for their resistance to stem canker (Diaporthe phaseolorum f.sp. meridionalis), frogeye leaf spot (Cercospora sojina Hara), and powdery mildew (Microsphaera diffusa Cke. & Pk.). All genetic materials studied were resistant to stem canker. FT-Cristalina RCH and Doko-RC and their TP and TN lines were resistant to frogeye leaf spot. IAC-12 and its derived lines not only presented a higher disease index, but also the derived lines, TP and TN, were more susceptible, indicating the loss of genes for disease resistance in the backcrosses. There was no association between the elimination of lipoxygenases from the seeds with the resistance to frogeye leaf spot. In relation to the powdery mildew, TP or TN lines presented similar or higher resistance than their respective recurrent parents whose susceptibility appeared in the following order: IAC-12, less susceptible, Doko-RC, intermediate and FT-Cristalina RCH, more susceptible.
RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
Martins Filho, Sebasti?o;Sediyama, Carlos Sigueyuki;Moreira, Maurilio Alves;Barros, Everaldo Gon?alves de;
Genetics and Molecular Biology , 2002, DOI: 10.1590/S1415-47572002000300012
Abstract: the soybean (glycine max (l.) merrill) frogeye leaf spot is caused by the fungus cercospora sojina hara and is a widespread disease in brazil and other countries, causing severe losses in grain yield and also affecting seed quality. the availability of dna markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. bulked segregant analysis was applied to 3 f2 populations derived from crosses between the resistant cultivars parana, cristalina and uberaba, and the susceptible cultivar bossier. in the cross 'parana' x 'bossier', 2 rapd markers were identified, csopa1800c and csopa21,250c, located at 4.4 ± 1.8 centimorgans (cm) and 3.4 ± 1.7 cm respectively from the resistance locus. dna fragments of similar molecular weight were observed in the population derived from the cross 'cristalina' x 'bossier' at 2.3 ± 1.2 and 4.7 ± 1.5 cm from the resistance locus, respectively. in the offspring of the cross 'uberaba' x 'bossier', a dna fragment corresponding to marker csopa1800c was detected at 5.6 ± 2.1 cm from the resistance locus. although marker csopa21,250c was not observed in this population, an additional marker was detected (csoub11,100c) at 6.7 ± 2.2 cm from the resistance locus. the 1,250 bp fragment of csopa21,250c was cloned and converted into a scar marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars cristalina and parana. markers csopa1800c, csopa21,250c and csoub11,100c were mapped to soybean linkage group j with the aid of known ssrs linked to the rcs3 locus, indicating that the rapd and scar markers identified in our research also tag this resistance gene.
RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean  [cached]
Martins Filho Sebasti?o,Sediyama Carlos Sigueyuki,Moreira Maurilio Alves,Barros Everaldo Gon?alves de
Genetics and Molecular Biology , 2002,
Abstract: The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross 'Cristalina' x 'Bossier' at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross 'Uberaba' x 'Bossier', a DNA fragment corresponding to marker CSOPA1(800C) was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA2(1,250C) was not observed in this population, an additional marker was detected (CSOUB1(1,100C)) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA2(1,250C) was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1(800C), CSOPA2(1,250C) and CSOUB1(1,100C) were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene.
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