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A combined phase I and II open label study on the effects of a seaweed extract nutrient complex on osteoarthritis
Stephen P Myers, Joan O’Connor, J Helen Fitton, et al.
Biologics: Targets and Therapy , 2010, DOI: http://dx.doi.org/10.2147/BTT.S8354
Abstract: combined phase I and II open label study on the effects of a seaweed extract nutrient complex on osteoarthritis Original Research (7562) Total Article Views Authors: Stephen P Myers, Joan O’Connor, J Helen Fitton, et al. Published Date February 2010 Volume 2010:4 Pages 33 - 44 DOI: http://dx.doi.org/10.2147/BTT.S8354 Stephen P Myers1,2, Joan O’Connor1,2, J Helen Fitton3, Lyndon Brooks4, Margaret Rolfe4, Paul Connellan5, Hans Wohlmuth2,5,6, Phil A Cheras1,2, Carol Morris5 1NatMed-Research, 2Centre for Health and Wellbeing, 4Graduate Research College, 5Centre for Phytochemistry and Pharmacology, 6Medicinal Plant Herbarium, Southern Cross University, Lismore, NSW, Australia; 3Marinova Pty Ltd, Hobart, Tasmania, Australia Background: Isolated fucoidans from brown marine algae have been shown to have a range of anti-inflammatory effects. Purpose: This present study tested a Maritech extract formulation, containing a blend of extracts from three different species of brown algae, plus nutrients in an open label combined phase I and II pilot scale study to determine both acute safety and efficacy in osteoarthritis of the knee. Patients and methods: Participants (n = 12, five females [mean age, 62 ± 11.06 years] and seven males [mean age, 57.14 ± 9.20 years]) with a confirmed diagnosis of osteoarthritis of the knee were randomized to either 100 mg (n = 5) or 1000 mg (n = 7) of a Maritech extract formulation per day. The formulation contained Maritech seaweed extract containing Fucus vesiculosis (85% w/w), Macrocystis pyrifera (10% w/w) and Laminaria japonica (5% w/w) plus vitamin B6, zinc and manganese. Primary outcome was the average comprehensive arthritis test (COAT) score which is comprised of four sub-scales: pain, stiffness, difficulty with physical activity and overall symptom severity measured weekly. Safety measures included full blood count, serum lipids, liver function tests, urea, creatinine and electrolytes determined at baseline and week 12. All adverse events were recorded. Results: Eleven participants completed 12 weeks and one completed 10 weeks of the study. Using a multilevel linear model, the average COAT score was reduced by 18% for the 100 mg treatment and 52% for the 1000 mg dose at the end of the study. There was a clear dose response effect seen between the two treatments (P ≤ 0.0005) on the average COAT score and each of the four COAT subscales (pain, stiffness, difficulty with physical activity and overall symptom severity) (P ≤ 0.05). The preparation was well tolerated and the few adverse events were unlikely to be related to the study medication. There were no changes in blood parameters measured over the course of the study with the exception of an increase in serum albumin which was not clinically significant. Conclusion: The seaweed extract nutrient complex when taken orally over twelve weeks decreased the symptoms of osteoarthritis in a dose-dependent manner. It was demonstrated to be safe to use over the study period a
A combined phase I and II open label study on the effects of a seaweed extract nutrient complex on osteoarthritis  [cached]
Stephen P Myers,Joan O’Connor,J Helen Fitton
Biologics: Targets and Therapy , 2010,
Abstract: Stephen P Myers1,2, Joan O’Connor1,2, J Helen Fitton3, Lyndon Brooks4, Margaret Rolfe4, Paul Connellan5, Hans Wohlmuth2,5,6, Phil A Cheras1,2, Carol Morris51NatMed-Research, 2Centre for Health and Wellbeing, 4Graduate Research College, 5Centre for Phytochemistry and Pharmacology, 6Medicinal Plant Herbarium, Southern Cross University, Lismore, NSW, Australia; 3Marinova Pty Ltd, Hobart, Tasmania, AustraliaBackground: Isolated fucoidans from brown marine algae have been shown to have a range of anti-inflammatory effects.Purpose: This present study tested a Maritech extract formulation, containing a blend of extracts from three different species of brown algae, plus nutrients in an open label combined phase I and II pilot scale study to determine both acute safety and efficacy in osteoarthritis of the knee. Patients and methods: Participants (n = 12, five females [mean age, 62 ± 11.06 years] and seven males [mean age, 57.14 ± 9.20 years]) with a confirmed diagnosis of osteoarthritis of the knee were randomized to either 100 mg (n = 5) or 1000 mg (n = 7) of a Maritech extract formulation per day. The formulation contained Maritech seaweed extract containing Fucus vesiculosis (85% w/w), Macrocystis pyrifera (10% w/w) and Laminaria japonica (5% w/w) plus vitamin B6, zinc and manganese. Primary outcome was the average comprehensive arthritis test (COAT) score which is comprised of four sub-scales: pain, stiffness, difficulty with physical activity and overall symptom severity measured weekly. Safety measures included full blood count, serum lipids, liver function tests, urea, creatinine and electrolytes determined at baseline and week 12. All adverse events were recorded.Results: Eleven participants completed 12 weeks and one completed 10 weeks of the study. Using a multilevel linear model, the average COAT score was reduced by 18% for the 100 mg treatment and 52% for the 1000 mg dose at the end of the study. There was a clear dose response effect seen between the two treatments (P ≤ 0.0005) on the average COAT score and each of the four COAT subscales (pain, stiffness, difficulty with physical activity and overall symptom severity) (P ≤ 0.05). The preparation was well tolerated and the few adverse events were unlikely to be related to the study medication. There were no changes in blood parameters measured over the course of the study with the exception of an increase in serum albumin which was not clinically significant.Conclusion: The seaweed extract nutrient complex when taken orally over twelve weeks decreased the symptoms of osteoarthritis in a dose-de
Immunomodulatory activity of aqueous extract of Ocimum sanctum in rat  [PDF]
R.Caroline Jeba,Rama Vaidyanathan,G.Rameshkumar
International Journal of Pharmaceutical and Biological Research , 2011,
Abstract: Background: Biochemical, haematological and Immunomodulatory effect of Ocimum sanctum in rat was studied. Objective: To evaluate the immunomodulatory effect of Ocimum sanctum in rat Methods: Aqueous extract of Ocimum sanctum were administered oraly at doses of 100, 200 mg/kg/day for 45 days in wistar albino rats. Immunomodulatory effect and biochemical and haematological changes were tested by standard methods. Results: Aqueous extract of the Ocimum sanctum showed increasing antibody production in dose dependent manner. It enhance the production of RBC, WBC and haemoglobin. It dose not affect the biochemical parameters. Conclusion: An oral administration of the aqueous extract of O. sanctum showed immunomodulatory effect in rat.
Immunomodulatory effect of methanol extract of Solanum xanthocarpum fruits
Rokeya Sultana,Salma Khanam,,Kshama Devi
International Journal of Pharma Sciences and Research , 2011,
Abstract: Purpose : To study the immunomodulatory activity of methanol extracts of fruits of Solanum xanthocarpum (Solanaceae) on Swiss albino mice.Methods Model -Cyclophosphamide induced immunosuppression model Animal used - Swiss albino mice. Dose – Cyclophosphamide-30mg/kg body weight, i.p route. Extract-100mg/kg body weight, oral route. The extent of protection against immunosuppression caused by yclophosphsmide was evaluated after 14 days of drug administration, by estimating hematological parameter and neutrophil adhesion test.Results Methanol extracts of fruits of Solanum xanthocarpum showed pronounced immunoprotective activity by increasing the depleted levels of total WBC count and RBC, % Hb, and % neutrophils adhesion.Conclusions The extract was found to be effective immunomodulatory agents.
Immunomodulatory potential of ethanol extract of spilanthus acmella leaves  [PDF]
Yadav Rajesh,Kharya Dhar Murli, Yadav Nita, Savadi Rudraprabhu
International Journal of Biological and Medical Research , 2011,
Abstract: Aims: Ethanol extract of leaves of Spilanthes acmella (SAEE) was evaluated for its immunomodulatory potential using various models like neutrophil adhesion (NA) test, haemagglutinating antibody (HA) titre and delayed type hypersensitivity (DTH) response in rats. Methods: The coarse powder (40-mesh) of shade dried leaves (500g) of Spilanthes acmella was subjected to maceration using 95% ethanol. Preliminary phytochemical tests were conducted for SAEE to identify the various phytoconstituents. Two doses of SAEE were selected and SAEE was administered orally at doses of 250 mg/kg body weight and 500 mg/kg body weight to healthy rats. The assessment of immunomodulatory potential was carried out by testing the humoral (antibody titre) and cellular (foot pad swelling) immune responses to the antigenic challenges with sheep RBC (SRBC) and by neutrophil adhesion test. Clean tap water was served as a control in all the tests. Results: Orally administered SAEE showed a significant increase in neutrophil adhesion, haemagglutinating antibody titre (HAT) and delayed type hypersensitivity (DTH) response. In rats immunized with sheep RBC, SAEE enhanced the humoral antibody response to the antigen and significantly potentiated the cellular immunity by facilitating the footpad thickness response to sheep RBC in sensitized rats. With a dose of 500 mg/kg body weight the values of NA, HAT and DTH responses were statistically significant as compared to control. Conclusion: The study demonstrates the immunomodulatory potential of Spilanthes acmella in rats. The responses were statistically significant when they were compared to control.
Impact of foliar application of seaweed extract on growth, yield and quality of potato (Solanum tuberosum L.)
Muhammad Wasim Haider, Chaudhary Muhammad Ayyub, Muhammad Aslam Pervez, Habat Ullah Asad, Abdul Manan, Syed Ali Raza and Irfan Ashraf
Soil & Environment , 2012,
Abstract: A field trial was carried out in 2010 to investigate the effect of foliar application of seaweed extract “Primo” as an organic biostimulant on potato cv. ‘Sante’. Foliar application of seaweed extract was carried out at different growth stages of the crop (i.e. 30 days; 45 days; 60 days; 30 and 45 days; 30 and 60 days; 45 and 60 days; 30, 45 and 60 days after sowing). Control plants were sprayed with water without seaweed extract. A significant improvement in growth, yield and tuber quality of potato was observed where treatment was applied. The highest tuber yield was recorded with applications of seaweed extract at 30 + 60 days interval after planting. The treatment also improved nitrogen, total soluble solids and protein contents of the potato tubers. The results of the study concluded a positive response of potato plant growth and yield to the foliar application of seaweed extract.
DEVELOPMENTAL CHANGES IN THE GERMINATION, GROWTH AND CHLOROPHYLLASE ACTIVITY OF VIGNA MUNGO L. USING SEAWEED EXTRACT OF ULVA RETICULATA FORSSKAL.  [PDF]
Ganapathy Selvam G.,Balamurugan M.,Thinakaran T.,Sivakumar K
International Research Journal of Pharmacy , 2013,
Abstract: The effect of seaweed extract prepared from Ulva reticulata on seed germination, seedling growth and chlorophyllase activity of Vigna mungo L. was studied. 100% germination was recorded in the seeds treated with lower concentration of seaweed extract. The V. mungo seeds soaked with lower concentrations of the seaweed extracts showed higher rates of germination, while the higher concentrations of the extracts inhibited the germination.
Immunomodulatory effect of Hawthorn extract in an experimental stroke model
Chinnasamy Elango, Sivasithambaram Devaraj
Journal of Neuroinflammation , 2010, DOI: 10.1186/1742-2094-7-97
Abstract: After 15 days of treatment with Hawthorn extract [100 mg/kg, pretreatment (oral)], male Sprague Dawley rats underwent transient MCAO for 75 mins followed by reperfusion (either 3 or 24 hrs). We measured pro-inflammatory cytokines (IL-1β, TNF-α, IL-6), ICAM-1, IL-10 and pSTAT-3 expression in the brain by appropriate methods. We also looked at the cytotoxic T cell sub-population among leukocytes (FACS) and inflammatory cell activation and recruitment in brain (using a myeloperoxidase activity assay) after ischemia and reperfusion (I/R). Apoptosis (TUNEL), and Bcl-xL- and Foxp3- (Treg marker) positive cells in the ipsilateral hemisphere of the brain were analyzed separately using immunofluorescence.Our results indicate that occlusion followed by 3 hrs of reperfusion increased pro-inflammatory cytokine and ICAM-1 gene expressions in the ipsilateral hemisphere, and that Hawthorn pre-treatment significantly (p ≤ 0.01) lowered these levels. Furthermore, such pre-treatment was able to increase IL-10 levels and Foxp3-positive cells in brain after 24 hrs of reperfusion. The increase in cytotoxic T cell population in vehicle rats after 24 hrs of reperfusion was decreased by at least 40% with Hawthorn pretreatment. In addition, there was a decrease in inflammatory cell activation and infiltration in pretreated brain. Hawthorn pretreatment elevated pSTAT-3 levels in brain after I/R. We also observed an increase in Bcl-xL-positive cells, which in turn may have influenced the reduction in TUNEL-positive cells compared to vehicle-treated brain.In summary, Hawthorn extract helped alleviate pro-inflammatory immune responses associated with I/R-induced injury, boosted IL-10 levels, and increased Foxp3-positive Tregs in the brain, which may have aided in suppression of activated inflammatory cells. Such treatment also minimizes apoptotic cell death by influencing STAT-3 phosphorylation and Bcl-xL expression in the brain. Taken together, the immunomodulatory effect of Hawthorn extract m
Immunomodulatory Activity of the Methanol Extract of Amorphophallus campanulatus (Araceae) Tuber
A.S Tripathi, V Chitra, N.W Sheikh, D.S Mohale, A.P Dewan
Tropical Journal of Pharmaceutical Research , 2010,
Abstract: Purpose: Traditionally, Amorphophallus campanulatus tuber is used for the treatment of enlarged spleen, rheumatism and tumour. Therefore, this study was designed to evaluate the immunomodulatory activity of the plant material. Method: The effect of the methanol extract (ME) of Amorphophallus campanulatus tuber on immunological function in mice was studied using charcoal clearance, spleen index and delayed-type hypersensitivity (DTH) response models. The extract was administered orally at doses of 250 and 500 mg/kg. Results: The extract exhibited immunomodulatory activity by causing a significant decrease in charcoal clearance, spleen index and delayed-type hypersensitivity (DTH) response. Conclusion: Amorphophallus campanulatus caused decreases in the charcoal clearance rate and cellular immunity by facilitating the footpad thickness response to sheep red blood cell (RBC) in sensitized mice.
Evaluation of immunomodulatory potential of ethanolic extract of Roscoea procera rhizomes in mice  [cached]
Sahu Mahesh,Mali Prashant,Waikar Shekhar,Rangari Vinod
Journal of Pharmacy and Bioallied Sciences , 2010,
Abstract: Purpose : The aim of present study was to evaluate immunomodulatory potential of ethanolic extract of Roscoea procera (Zingiberaceae) rhizomes by using delayed-type hypersensitivity (DTH) and carbon clearance method in comparison to standard established immunosuppressant drug, cyclophosphamide (30 mg/kg, i.p.) in mice. Material and Methods : The extract was comprised to acute toxicity (OECD-423 guideline), DTH and carbon clearance method for their immunomodulatory potential. Ethanolic extract of Roscoea procera rhizomes administered orally at doses 300 mg/kg and 600 mg/kg, p.o. to mice. Result and Conclusion : Result of our study revealed that, the foot pat thickness of ethanolic extract group (P<0.05) significantly enhanced the production of circulating antibody titre in response to Sheep red blood cells (SRBC) and phagocytic functions of mononuclear macrophages and non-specific immunity. Result were also supported by serological and haematological tests data. Hence, the present investigation reveals that, ethanolic extract of Roscoea procera rhizomes possesses immunostimulant properties. Further studies to identify the active moieties and elucidation of the mechanism of action are recommended.
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