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Influence of Explant Source and Medium Ingredients on Culture Responses of Microsperma Lentil Cultivar Masoor-85  [PDF]
N. Altaf,J. Iqbal,M. S. Ahmad
Pakistan Journal of Biological Sciences , 1998,
Abstract: Culture responses of 9 lentil explants were compared in various media having different ingredients. The most promising were the scrapped axillary portions of basal nodes for enhanced regeneration. The optimum size of the explant was also determined. The callogenesis response varied with the explant and ranged between 0.3 - 0.5 cm. Among the sugars tested, sucrose was the best for callussing. Auxin 2,4-D induced callussing but cytokinin enhanced formation of shoot primordia in explants. Both BA and K promoted multiple shoot formation but K and GA gave more shoot buds. Cytokinins with auxins gave callussing. Callus was also induced in explants when grown in K (10 mg/l) + GA (1mg/l) containing medium in the dark. When callus was shifted to light it started regeneration as green dots. The addition of seed extract to K and GA medium considerably enhanced regenerations.
Some Aspects of Adventitious Rooting in Microsperma Lentil CV-Masoor-85  [PDF]
Nafees Altaf,Javed Iqbal,M. Salih Ahmad
Pakistan Journal of Biological Sciences , 1999,
Abstract: Presence of auxins and high humidity in the initial stages of growth in vitro of 10-15 days old shoot cuttings is very essential for adventitious rooting in microsperma lentil in cultivar Masoor-85. The type and quantity of auxins is important. The basal segments of stem have more chances of rooting which gradually decreases for apical segments. Also age of shoots in culture has influence on rooting. Rooting is with normal polarity. Boron ( 30 mg/l) and MS (1/10 strength) salts in diluted concentration helps root induction and development. Moist peat and filter paper bridge both support rooting.
Agromorphological Traits Variability of the Ethiopian Lentil and Exotic Genotypes  [PDF]
Fikru Mekonnen,Firew Mekbib,Shiv Kumar,Seid Ahmed,Tilak R. Sharma
Advances in Agriculture , 2014, DOI: 10.1155/2014/870864
Abstract: Understanding the genetic relationships and diversity of Ethiopian lentil in relation to lentil from other countries is important in attempting to widen the genetic base of germplasm in the country. The objectives of this study were to generate information on agromorphological variability, to estimate PCV, GCV, heritability, and expected genetic advance of quantitative traits of lentil. 228 genotypes with different population types were studied for 11 agromorphological traits and rust disease severity score for two seasons (2011-2012) over three locations. The analysis of variance showed highly significant variations ( ) among genotypes for all characters studied. As per genetic parameter values, four groups of character were deduced. It is inferred that the exotic genotypes introduced from ICARDA showed rich genetic bases for 100-seed weight, number of seeds per plant, seed weight per plant, resistance source for rust, and high yielder in high yielding environment, where rainfall is not a major problem. Use the Ethiopian accessions for developing cultivars that could be used in double cropping and drought prone areas. 1. Introduction Lentil (Lens culinaris Medik. subsp. culinaris) is a short, slender annual cool-season food legume. The center of origin of lentil is the Near East where it was first domesticated in the Fertile Crescent around 7000 BC [1]. According to Barulina [2], two varietal types based on seed size were recognized: the small-seeded microsperma and the macrosperma with relatively large flattened pods. The largest lentil producer is India, followed by Canada and Turkey, accounting for 68% of the global production [3]. In Africa, Ethiopia, Morocco, and Tunisia are the leading producers, but 61% of the areas and 68% of the production belong to Ethiopia [4]. Lentil is an important dietary source of macro- and micronutrients for both rural and urban dwellers. It provides sufficient amounts of the most essential amino acids to meet nutrient requirements. It is a cash crop fetching the highest price in domestic market compared to all other food legumes and major cereal crops [5]. Global, African, and Ethiopian lentil productivity is about 887?kg/ha, 644?kg/ha [3], and 1168?kg/ha, respectively [6]. Biotic and abiotic factors limit lentil productivity and seed quality. The major yield gap contributing factors are insect pests, diseases, low yielding landraces grown by farmers, and the narrow genetic base [7, 8]. Prior knowledge of genetic variability and characterization of genetic resources within the germplasm available at a gene bank has an
Somaclonal Variation in Microsperma Lentil (Lens culinaris Medik)  [PDF]
Nafees Altaf,Javed Iqbal,M.Salih Ahmad
Pakistan Journal of Biological Sciences , 1999,
Abstract: Shoot apices of Masoor-85 seedlings (3-4 days old) induced proliferated callus masses under dark conditions, were induced to proliferate callus masses. The callus was induced under dark conditions in MS medium containing K (10 mg/l), GA (1mg/l) with 5-10 per cent lentil seed extract. The shoots were regenerated by transferring the callus culture in light. The shoots were cut and rooted to have plantlets that were finally grown in the field in winter season. These plants were R0 generation. The first selections were made from R0 and seeds were collected. These seeds were grown for R1 generation, whose seeds were selected for R2 generation and so on. Some selected variants were stable till R5 generation and were called somaclonal. Variants for more pods and large seed were selected.
Comparative Growth and Pathogenicity of Geographical Isolates of Sclerotinia sclerotiorum on Lentil Genotypes  [PDF]
C. Akem,M. Bellar,B. Bayaa
Plant Pathology Journal , 2006,
Abstract: Isolates of Sclerotinia sclerotiorum were collected from infected lentil plants from 2 agro-ecological zones of Syria and used to study their comparative growth on culture media and pathogenicity on different lentil genotypes. The growth studies were carried out on Potato Dextrose Agar (PDA) growth media under laboratory conditions. Mycelial radial growth and sclerotial production were the parameters used to compare the isolates. Pathogenicity studies were carried out with selected isolates on 10 lentil genotypes, infected as detached shoots and as whole potted-plants in the plastic house. The isolates showed considerable variation in cultural characteristics through mycelial growth, mycelial pigmentation and sclerotial production in the media plates. There were significant differences in the growth and sclerotial production of most of the isolates, but no apparent correlation between mycelial growth and sclerotial production among the isolates. Genotype by isolate interactions was significant for the isolates tested for pathogenicity. These interactions, however, appeared to be caused by differences in virulence of the isolates and did not suggest the occurrence of distinct pathogenic races of the pathogen isolates.
Studies on Variability of Lentil Genotypes in Southeastern Anatolia of Turkey
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2008,
Abstract: Sixty-four lentil genotypes from ICARDA were evaluated at Diyarbakir, Turkey in 2003/2004 season. Eleven characters were studied. Days to maturity ranged from 188 to 196 days, and some genotypes were earlier than the local check, or same. Total 42 genotypes, suitable for mechanical harvest, were taller than 25 cm plant height. Seed yield per plant varied from 0.5 g to 2.366 g. The genotypes for cold tolerance were determined, and the genotypes of 1-3 scale were selected future lentil-breeding programme. Grain yield ranged from 776.8 kg/ha for FLIP 96-47L to 3242.3 kg/ha for FLIP 2004-49L. Grain yield was positively correlated with days to maturity, biological yield per plant, and plant height, number of seeds and pods per plant and seed yield per plant, negatively correlated with days to flowering and number of branches per plant.
Determining Rank and Stability of Lentil Genotypes in Rainfed Condition by Nonparametric Statistics  [cached]
R. Karimizadeh,M. Safikhani Nasimi,M. Mohammadi,F. Seyyedi
Journal of Science and Technology of Agriculture and Natural Resources , 2008,
Abstract: One of the applications of Non-Parametric methods is determination of genotypes rank in different environments, which is also used as a measuring stability. A stable genotype shows similar ranks across different environments and has minimum rank variance in different environments. Non-Parametric Stability Statistics require no statistical assumptions about the distribution of the phenotypic values and are easy to use. This study was carried out to determine the ranks of 10 Lentil genotypes (Lens culinaris Medikus) across ten environments in 2002-2004, using a randomized complete block design with four replications. Analysis of Thennarasu non-parametric statistics showed that genotypes 8 and 9 had high stability by NP(1) statistic and genotypes 9, 8 and 1 had stable yield in NP(2) method. Result of the NP(3) statistic was similar to NP(1) statistic. NP(4) statistic selected genotypes 9 and 1 as the most stable genotypes and ultimately NP(5) statistic introduced 9 and 1 genotypes as stable genotypes in this experiment. Also analysis of Nassar and Huhn non-parametric statistics revealed that genotypes 1 and 2 were most stable and well adapted across ten environments. In addition, it was concluded that plots obtained by both mean yield (kg ha-1) vs.Si(1) and mean yield (kg ha-1) vs. Si(2) values could enhance visual efficiency of selection based on genotype × environment interaction. According to these configurations, genotypes in section 1 can be considered as stable and well adapted to all environments, having general adaptable ability. For recognition a daptability,Si(1) and Si(2) take preferred over other non-parametric statistics.
Phenological, Nutritional and Molecular Diversity Assessment among 35 Introduced Lentil (Lens culinaris Medik.) Genotypes Grown in Saudi Arabia  [PDF]
Salem S. Alghamdi,Altaf M. Khan,Megahed H. Ammar,Ehab H. El-Harty,Hussein M. Migdadi,Samah M. Abd El-Khalik,Aref M. Al-Shameri,Muhammad M. Javed,Sulieman A. Al-Faifi
International Journal of Molecular Sciences , 2014, DOI: 10.3390/ijms15010277
Abstract: Morphological, nutritional and molecular analyses were carried out to assess genetic diversity among 35 introduced lentil genotypes ( Lens culinaris Medik.). The genotypes exhibited significant differences for their field parameters and some of them showed noticeable superiority. The nutritional and proximate analysis showed that some genotypes were excellent sources of proteins, essential amino acids, minerals, anti-oxidants, total phenolic contents (TPC) and total flavonoid contents (TFC) and hence, highlights lentil nutritional and medicinal potential. Sequence-related amplified polymorphism (SRAP) and amplified fragments length polymorphism (AFLP) markers were used to estimate the genetic variability at the molecular level. The existence of a considerable amount of genetic diversity among the tested lentil genotypes was also proven at the molecular level. A total of 2894 polymorphic SRAP and 1625 AFLP loci were successfully amplified using six SRAP and four AFLP primer pair combinations. Polymorphism information content (PIC) values for SRAP and AFLP markers were higher than 0.8, indicating the power and higher resolution of those marker systems in detecting molecular diversity. UPGMA (unweighted pair group method with arithmetic average) cluster analysis based on molecular data revealed large number of sub clusters among genotypes, indicating high diversity levels. The data presented here showed that FLIP2009-64L and FLIP2009-69L could be used as a significant source of yield, total protein, essential amino acids, and antioxidant properties. The results suggest potential lentil cultivation in the central region of Saudi Arabia for its nutritional and medicinal properties, as well as sustainable soil fertility crop.
Responses of Wheat Genotypes as Affected by Different Sowing Dates
F. Aslani,M.R. Mehrvar
Asian Journal of Agricultural Sciences , 2012,
Abstract: A field experiment was conducted Seed and Plant Improvement Institute, Karaj (Iran) for two seasons, 2007-08 and 2008-09, on farmer’s fields to investigate the effect of two sowing dates; optimum sowing date (1st November) and late sowing date (20th November) on yield and yield components of eight wheat genotypes. The results showed that the optimum sowing produced higher grain and biomass yields, 1000- grain weight, Spike per square meter compared to late sowing. Between grain yield and traits of biological yield, 1000- grain weight and grain per square meter were correlated positively.
Journal of Central European Agriculture , 2007,
Abstract: Fourteen lentil genotypes were tested for grain yield in Southeastern Anatolia ecological conditions, over our consecutive years to classify these cultivars for yield stability. Seed yield ranged from 1.903 t/ha to 1.367 t/ha. RM76, RM601 and RM152 showed regression coeffi cient above 1.00, but RM76 among these lines was consistently produced the highest yields. The unstable cultivars, RM601 and RM152 had the highest S2 values and high C.V. for grain yield.
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