oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
HEPATOPROTECTIVE EFFECT OF SOLENA AMPLEXICAULIS (TUBER) ON ACUTE CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY  [PDF]
Krishna Mohan G*
International Journal of Pharmacy and Technology , 2010,
Abstract: The hepatoprotective activity of methanolic extract of Solena amplexicaulis (SAME) (Lam.)Gandhi (Cucurbitaceae) at doses of 250 mg/kg and 500 mg/kg were evaluated by carbon tetrachloride(CCl4) intoxication in rats. The toxic group which received CCl4 (0.3 ml/kg of CCl4 dissolved in 1:1 ratio in olive oil by subcutaneous (s.c) alone exhibited significant increase in serum alanine aminotransferase(ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP) and total bilirubin (TB) levels. It also caused significant (P<0.001) decrease in protein levels. The groups receivedpretreatment of SAME at a dose of 500 mg/kg b.w.p.o. had controlled the AST, ALT, ALP and total bilirubin levels and the effects were comparable with standard drug (silymarin 100 mg/kg b.w.p.o).The total protein (TP) and albumin (ALB) levels were significantly increased in the animals received pretreatment of the extract at the higher dose level. The animals received pretreatment of the extractshown decreased necrotic zones and hepatocellular degeneration when compared to the liver exposed to CCl4 intoxication alone. Thus the histopathalogical studies also supported the protective effect ofthe extract.
HEPATOPROTECTIVE ACTIVITY OF METHANOLIC EXTRACT OF ECBOLIUM VIRIDE (FORSSK.) ALSTON ROOTS AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY  [PDF]
Ashoka Babu V.L,Ganeshan Arunachalam,Korlakunda Narasimha Jayaveera,Varadharajan Madhavan
International Research Journal of Pharmacy , 2012,
Abstract: In the present study, the methanolic extract of Ecbolium viride root was evaluated for its hepatoprotective effect against CCl4 induced hepatic injury in rats. Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALP, triglycerides, bilirubin, total proteins and liver weight were tested in both treated and untreated groups. CCl4 (1ml/kg) enhanced the SGPT, SGOT, ALP, triglycerides, liver weight and reduced total proteins significantly. Treatment with methanolic extract of Ecbolium viride roots (200mg/kg and 400mg/kg) has brought back the altered levels of altered levels of biochemical markers significantly to the near normal levels in the dose dependant manner.
Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats
Zim, M.C.A.;Silveira, T.R.;Schwartsmann, G.;Cerski, T.;Motta, A.;
Brazilian Journal of Medical and Biological Research , 2002, DOI: 10.1590/S0100-879X2002001100012
Abstract: few data are available in the literature regarding the effect of pentosan polysulfate (pps) on normal and fibrotic rat livers. in addition, the combination of pps and carbon tetrachloride (ccl4) has not been studied so far. the objective of this study was to assess the effect of pps on rat livers treated or not with ccl4 for the induction of liver fibrosis. the study consisted of four stages: 1) hepatic fibrosis induction with ccl4 (n = 36 rats); 2) evaluation of the effect of pps on ccl4-induced hepatic fibrosis (n = 36 rats); 3) evaluation of the effect of higher doses of pps in combination with ccl4 (n = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by pps (n = 18 rats) using the sodium pentobarbital test which indirectly evaluates hepatic microsomal enzyme activity in vivo. adult (60 to 70 days) male wistar rats weighing 180 to 220 g were used. all animals receiving 0.5 ml 8% ccl4 (n = 36) developed hepatic fibrosis, and after 8 weeks they also developed cirrhosis. no delay or prevention of hepatic fibrosis was observed with the administration of 5 mg/kg pps (n = 8) and 1 mg/kg pps (n = 8) 1 h after the administration of ccl4, but the increased hepatotoxicity resulting from the combination of the two substances caused massive hepatic necrosis in most rats (n = 45). pps (40 mg/kg) alone caused hepatic congestion only after 8 weeks, but massive hepatic necrosis was again observed in association with 0.5 ml ccl4 after 1 to 4 weeks of treatment. unexpectedly, sleeping time increased with time of pps administration (1, 2, or 3 weeks). this suggests that pps does not function as an activator of the hepatic microsomal enzymatic system. further studies are necessary in order to clarify the unexpected increase in hepatotoxicity caused by the combination of ccl4 and high doses of pps, which results in massive hepatic necrosis.
Potentiation of carbon tetrachloride hepatotoxicity by pentosan polysulfate in rats  [cached]
Zim M.C.A.,Silveira T.R.,Schwartsmann G.,Cerski T.
Brazilian Journal of Medical and Biological Research , 2002,
Abstract: Few data are available in the literature regarding the effect of pentosan polysulfate (PPS) on normal and fibrotic rat livers. In addition, the combination of PPS and carbon tetrachloride (CCl4) has not been studied so far. The objective of this study was to assess the effect of PPS on rat livers treated or not with CCl4 for the induction of liver fibrosis. The study consisted of four stages: 1) hepatic fibrosis induction with CCl4 (N = 36 rats); 2) evaluation of the effect of PPS on CCl4-induced hepatic fibrosis (N = 36 rats); 3) evaluation of the effect of higher doses of PPS in combination with CCl4 (N = 50 rats); 4) evaluation of the presence of an enzymatic inductor effect by PPS (N = 18 rats) using the sodium pentobarbital test which indirectly evaluates hepatic microsomal enzyme activity in vivo. Adult (60 to 70 days) male Wistar rats weighing 180 to 220 g were used. All animals receiving 0.5 ml 8% CCl4 (N = 36) developed hepatic fibrosis, and after 8 weeks they also developed cirrhosis. No delay or prevention of hepatic fibrosis was observed with the administration of 5 mg/kg PPS (N = 8) and 1 mg/kg PPS (N = 8) 1 h after the administration of CCl4, but the increased hepatotoxicity resulting from the combination of the two substances caused massive hepatic necrosis in most rats (N = 45). PPS (40 mg/kg) alone caused hepatic congestion only after 8 weeks, but massive hepatic necrosis was again observed in association with 0.5 ml CCl4 after 1 to 4 weeks of treatment. Unexpectedly, sleeping time increased with time of PPS administration (1, 2, or 3 weeks). This suggests that PPS does not function as an activator of the hepatic microsomal enzymatic system. Further studies are necessary in order to clarify the unexpected increase in hepatotoxicity caused by the combination of CCl4 and high doses of PPS, which results in massive hepatic necrosis.
PROTECTIVE EFFECT OF NERIUM INDICUM ON CCl4 INDUCED HEPATOTOXICITY IN RATS  [cached]
Govind Patel
International Journal of Biomedical Research , 2011, DOI: 10.7439/ijbr.v1i4.68
Abstract: Methanolic flowers extract of Nerium indicum was evaluated for hepatoprotective in rats. The plant extract (500 and 1000 mg/kg, p.o.) showed a remarkable hepatoprotective activity against carbon tetrachloride induced hepatotoxicity in liver tissues. Carbon tetrachloride induced a significant rise in Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT) and alkaline phosphatase (ALP). Treatment of rats with different doses of plant extract (500 and 1000 mg/kg) significantly (P<0.001) altered serum marker enzymes levels to against carbon tetrachloride treated rats. The activity of the extract at dose of 300 mg/kg was comparable to the standard drug, silymarin (100 mg/kg, p.o.). Histopathological changes of liver sample were compared with respective control. Results indicate the hepatoprotective properties of Nerium indicum against carbon tetrachloride induced hepatotoxicity in rats.
PROTECTIVE EFFECT OF NERIUM INDICUM ON CCl4 INDUCED HEPATOTOXICITY IN RATS
Govind Patel
International Journal of Biomedical Research , 2013, DOI: 10.7439/ijbr.v1i4.68
Abstract: Methanolic flowers extract of Nerium indicum was evaluated for hepatoprotective in rats. The plant extract (500 and 1000 mg/kg, p.o.) showed a remarkable hepatoprotective activity against carbon tetrachloride induced hepatotoxicity in liver tissues. Carbon tetrachloride induced a significant rise in Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT) and alkaline phosphatase (ALP). Treatment of rats with different doses of plant extract (500 and 1000 mg/kg) significantly (P<0.001) altered serum marker enzymes levels to against carbon tetrachloride treated rats. The activity of the extract at dose of 300 mg/kg was comparable to the standard drug, silymarin (100 mg/kg, p.o.). Histopathological changes of liver sample were compared with respective control. Results indicate the hepatoprotective properties of Nerium indicum against carbon tetrachloride induced hepatotoxicity in rats.
Ideal hepatotoxicity model in rats using Carbon Tetrachloride (CCl4)
AJ Alhassan, MJ Sule, SA Aliyu, MD Aliyu
Bayero Journal of Pure and Applied Sciences , 2009,
Abstract: A study to produce ideal Hepatotoxicity rats’ models using varying concentrations of carbon tetrachloride (CCl4) was carried out. A total of seventy five rats were divided into five (5) groups of twenty five (25) rats each; rats in group I are negative control, were not induced with lipid peroxidation. Rats in groups II, III, IV and V were induced with lipid peroxidation and liver damage using, 90, 105 and 120mg/Kg body weight CCl4 respectively. Five (5) rats were removed from each group after 48 hours, 72 hours (3 days) and 144 hours (6 days) of inducement with CCl4 and sacrificed for blood sample collection. Sera obtained were analyzed for alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) activities, and for Malondialdehyde (MDA), total protein (TP) and albumin (ALB) concentrations. After 48hours, rats treated with various concentrations of CCl4 had mean serum activities/ levels of AST, ALT, ALP, MDA, TP and ALB significantly higher (p< 0.05) than normal rats. The control group had 39.5±0.71, 11.5±2.12, 36±7.07U/L, 0.0769 ± 0.015 μM, 43.5±3.54, 19 .00 ± 2.83 g/L respectively. Rats treated with 75mg of CCl4 showed activities/level non significant (p>0.05) increase of serum AST, ALT, ALP, and MDA after 96 hours of CCl4 treatment compared with control group. However, rats treated with 90, 105 and 120mg/kg of CCl4 showed persistently high serum AST, ALT, ALP in U/L, MDA in μM/L, TP and ALB in g/L. even at 144 hours after the treatment. Ideal hepatotoxicity rat model using CCl4 requires a dosage that can cause massive liver damage. Key Words: carbon tetrachloride, hepatotoxicity rats modeling, natural healing.
HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ALTHAEA OFFICINALIS LINN AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY ON ALBINO WISTAR RATS
Jabbar Zoobi,Ali Mohd
International Research Journal of Pharmacy , 2011,
Abstract: In Indian traditional system of medicine, herbal remedies are prescribed for the treatment of various diseases including liver diseases. The present study was aimed to investigate the hepatoprotective activity of the ethanolic extract of Althaea officinalis against Carbon tetrachloride induced hepatotoxicity in rats. Liver function were assessed by the determination of SGPT and SGOT studies. The serum biochemical analysis results suggested that the use of ethanolic extract of Althaea officinalis exhibited significant protective effect from hepatic damage in CCl4 induced hepatotoxicity model.
Hepatoprotective effects of berberine on carbon tetrachloride-induced acute hepatotoxicity in rats
Yibin Feng, Ka-Yu Siu, Xingshen Ye, Ning Wang, Man-Fung Yuen, Chung-Hang Leung, Yao Tong, Seiichi Kobayashi
Chinese Medicine , 2010, DOI: 10.1186/1749-8546-5-33
Abstract: Sprague-Dawley rats aged seven weeks were injected intraperitoneally with 50% CCl4 in olive oil. Berberine was orally administered before or after CCl4 treatment in various groups. Twenty-four hours after CCl4 injection, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, serum and liver superoxide dismutase (SOD) activities were measured. Histological changes of liver were examined with microscopy.Serum ALT and AST activities significantly decreased in a dose-dependent manner in both pre-treatment and post-treatment groups with berberine. Berberine increased the SOD activity in liver. Histological examination showed lowered liver damage in berberine-treated groups.The present study demonstrates that berberine possesses hepatoprotective effects against CCl4-induced hepatotoxicity and that the effects are both preventive and curative. Berberine should have potential for developing a new drug to treat liver toxicity.Liver damage induced by carbon tetrachloride (CCl4) involves biotransformation of free radical derivatives, increased lipid peroxidation and excessive cell death in liver tissue [1,2]. This model of CCl4-induced liver injury has been widely used in new drug development for liver diseases.Berberine is a plant alkaloid present in many medicinal herbs, such as Hydrastis canadensis, Coptidis Rhizoma, Berberis aquifolium, Berberis aristata and Berberis vulgaris [3]. Coptidis Rhizoma (Huanglian), which is rich in berberine, exhibited hepatoprotective effects on CCl4-induced liver injury via scavenging the peroxidative products [4]. Antioxidative effects of Coptidis Rhizoma and its major active ingredient berberine against peroxynitrite-induced kidney damage were demonstrated in vitro and in vivo [5]. Previous studies reported that berberine inhibited inflammation [6] and low-density lipoprotein (LDL) oxidation [7]. Other studies found that berberine was a candidate drug for Alzheimer's disease [8] and cancer [9]. Berberine exhib
Studies on hepatoprotective activity of traditional ayurvedic formulation ‘Vidakana Choornam’ against carbon tetrachloride induced hepatotoxicity in albino rat
Jayarama Reddy, Gnanasekaran D, Ranganathan TV and Vijay D
International Journal of Pharmaceuticals Analysis , 2010,
Abstract: The study was carried out to ascertain the hepatoprotective activity of traditional ayurvedicformulation ‘Vidakana Choornam’ against carbon tetrachloride induced hepatotoxicity in albino rat. Effect ofcarbon tetrachloride and herbal products on liver weights was studied. Bilirubin level in serum, SerumGlutamate Pyruvate Transaminase (GPT) level in serum, Serum Glutamate Oxaloacetate Transaminase(GOT) level in serum and ALP level in serum were estimated in both control treatment. Histopathologicalstudies were also carried out. It was found that there was an increase in liver weight during carbontetrachloride treatment which may be due to accumulation of fat. The results of Vidakana Choornam’treatment against carbon tetrachloride induced hepatotoxicity revealed that there was considerablereduction in Bilirubin level in serum, Serum Glutamate Pyruvate Transaminase (GPT) level in serum, SerumGlutamate Oxaloacetate Transaminase (GOT) level in serum and ALP level in serum. The hepatoprotectiveactivity of this simple formulation was found to be as effective as Liv – 52, infact the activity on decreasingthe serum bilirubin level was much higher when compared to Liv – 52
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.