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Evaluación de la susceptibilidad de hongos endófitos aislados de rosa (rosa hybrida) a fungicidas comerciales Evaluating Susceptibility to Commercial Fungicide of Endophytic Fungi Isolated from Roses (Rosa hybrida)  [cached]
Corredor Perilla Ingrid Carolina,Cepero María Caridad,Restrepo Silvia
Revista Colombiana de Biotecnología , 2007,
Abstract: Los hongos endófitos han mostrado potencial como agentes de control biológico. Sin embargo, su aplicación en campos comerciales es aún limitada. En rosas, la continua aplicación de fungicidas en cultivos puede tener efectos deletéreos en el crecimiento de los endófitos. En este trabajo se evaluó la susceptibilidad de hongos endófitos aislados de Rosa hybrida a fungicidas utilizados comercialmente en el control de patógenos en el cultivo de rosa. Esto se realizó in vitro, mezclando diferentes concentraciones de fungicidas con medios estándares en el crecimiento de hongos endófitos y midiendo diariamente su crecimiento. La susceptibilidad de Botrytis cinerea (Cepa 3015), uno de los más importantes patógenos que afecta el cultivo de rosas en Colombia, se analizó de la misma forma. El 45,45% de los hongos endófitos evaluados demostraron susceptibilidad de crecimiento con grados de sensibilidad desde no sensibles (373,75%) hasta regularmente sensibles (348,75% - <61,25%) en las concentraciones evaluadas principalmente en fungicidas como boscalid, captan, iprodione y pyrimethanil. En el caso de fungicidas como carboxin más thiram, fludioxonil más ciprodinil, y prochloraz, se observaron grados de susceptibilidad de alta sensibilidad (<23,75%), inhibiendo totalmente el crecimiento de los hongos endófitos evaluados. En B. cinerea (Cepa 3015) se observó alta susceptibilidad a pyrimethanil, carboxin más thiram, fludioxonil más ciprodinil, y prochloraz. Aunque los controles de B. cinerea tuvieron mayores crecimientos, la mayoría de los crecimientos de los hongos endófitos evaluados en los medios enmendados en las dos concentraciones fueron superiores a los de este patógeno. El rango de susceptibilidad de hongos endófitos como las cepas 3002, 3003, 3004, 3005 y 3006 bajo los parámetros de análisis de este experimento, muestra su selección como hongos promisorios para programas de manejo integrado de plagas y enfermedades, teniendo en cuenta el momento, la frecuencia y la dosis de aplicación tanto de los fungicidas como de los hongos endófitos empleados. Fungal endophytes have shown their potential as biocontrol agents; however, their application in commercial fields remains limited. Continuously applying fungicides to crops (specifically to roses) may have harmful effects on endophyte growth. Endophytic fungi were isolated from R. hybrida and their susceptibility to fungicides regularly used for controlling important pathogens was analysed. This was performed in vitro, mixing several fungicide concentrations with standard medium for fungal endophytes; growth inhi
部分蔷薇和现代月季杂交亲和性
Compatibility of Crosses between Rosa multiflora and Rosa hybrida
 [PDF]

袁晓娜,刘瑞峰,张非亚,贾桂霞
- , 2015, DOI: 10.7606/j.issn.1004-1389.2015.02.022
Abstract: 为丰富国内自育品种并解决现代月季夏季病害严重开花不良的问题, 2011-2012年对蔷薇与月季品种杂交、杂种香水月季品种间杂交及不同类型月季品种间的63个组合的杂交进行研究,分析不同组合结实情况,发现杂种香水月季品种间杂交亲本亲和性较好,而蔷薇与月季品种的杂交亲和性较差。同时通过对38个组合的坐果情况跟踪观察,得出杂交果落果规律,月季杂交的落果时期主要出现在授粉后1月内及授粉3个月后。从亲和性分析这些组合的落果情况,同一类型品种间杂交果实严重落果期集中于果实成熟后期(授粉3个月后),而另外2类组合严重落果期在前(授粉1个月内)、后期(授粉3个月后)均有分布,与结实情况表现的亲和性一致。
In order to enrich domestic bred varieties of modern Rosa hybrida, solve the serious disease and bad flowering in summer, 63 crosses were made between once-flowering rose and different rose varieties from 2011 to 2012. The results of cross compatibility analysis showed that: intra-variety hybridization of Hybrid Tea Roses had higher fruit setting rate, and lower fruit setting rate was detected in the hybridization between wild rose and Rosa hybrida. Furthermore, fruit abscission in hybridization between same type of Rosa hybrida mainly occurred at the late stage (3 months after pollination), while for wild × cultivar crosses and different type of cultivar × cultivar crosses, serious abscission occurred at both stage (1 month after pollination and 3 month after pollination) , which were corresponded to the results of cross compatibility.
In Vitro Flowering from Cultured Nodal Explants of Rose (Rosa hybrida L.)  [cached]
Kantamaht KANCHANAPOOM,Nonlapan POSAYAPISIT,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2009,
Abstract: Roses are one of the world’s most important ornamentals for a long time and are most often used for ornamental, medicinal and aromatic purposes. The study reports in vitro multiple shoot formation and flower induction of Rosa hybrida L. cv. ‘Red Masterpiece’ with maximum number of 5 shoots per explant on MS medium supplemented with 3 mg/l BA and 1 mg/l kinetin, followed by flower induction on MS medium containing 2 mg/l BA for 9 weeks. The shoots readily rooted on MS medium devoid of growth regulators. Shoots cultured under various photoperiods did not flower. Rooted plantlets were hardened and established in pots with 100% survival.
Taxonomic revision of roses (Rosa L.) of selected areas in buffer zone of the Low Tatras National Park (Slovakia)  [PDF]
Anna So?tys-Lelek,Beata Barabasz-Krasny,Peter Turis,Ingrid Turisová
Modern Phytomorphology , 2012,
Abstract: The paper presents results of preliminary research carried out in 2011 in the area of two Protected Sites – CHA Jakub, CHA Kopec and Nature Reserve PR Mackov bok in the buffer zone of the Low Tatras National Park. Six native species and one native hybrid of roses were found there (1 from the Pimpinellifoliae section and 6 from the Caninae section) and their 13 varieties. The most numerous among them are Rosa canina var. dumalis Baker and R. × subcanina (H. Christ) R. Keller. Such great diversity of species and varieties insuch a small area (only 20.2 ha) proves considerable floristic richness, and occurrence of habitats preferred by roses.
Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes
Nan Ma,Lei Cai,Wangjin Lu,Hui Tan,Junping Gao
Science China Life Sciences , 2005, DOI: 10.1360/062004-37
Abstract: The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1-aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh-ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-induced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhibited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.
Response of cut rose flower (Rosa hybrida) to biofertilizer application in hydroponic system  [cached]
N. Mohseni Nik,H.R. Zabihi,A. Asgharzadeh
Journal of Science and Technology of Greenhouse Culture , 2012,
Abstract: Rose flower (Rosa hybrida) is appreciated for its beauty, fragrance and long period of flowering. To study the response of cut rose flower to bio-fertilizers, a factorial experiment was performed, with completely randomized design and four replications, in hydroponic greenhouse of Alandasht, Astane Ghods Garden, Mashhad, Iran, during 2009-2010. The first factor was six cultivars of rose flower including: Red ferover, Classic cezaanna, Rock feller, Rimini, Maroussia and Orange juice. The second factor was three biological fertilizers including biofarm, nitrajin and nitroxin and a no-inoculated treatment (control). In this experiment, traits such as leaf area, number of nodes, number of branches, first flowering occurrence, vase life and percentage of nitrogen, potassium and calcium were measured in each treatment and the means were compared by Duncan Multiple Range Test. Results showed that the effect of cultivar and compound effect of cultivar and fertilizer for number of nodes, number of branches, first flowering date, vase life, leaf area and nitrogen, potassium and calcium concentration were significant (P<0.05). The effect of bio-fertilizer treatments on leaf area and concentration of nutrients was significant (P<0.05). The highest concentration of nutrients was observed in Red ferover cultivar. Interaction effect of cultivar and bio-fertilizer treatments showed that in the Red ferover, Classic cezaanna and Maroussia cultivars, the fertilizer treatments increased nitrogen concentration in the leaves. The biofarm and nitroxin fertilizers increased leaf calcium concentration in Red ferover and Rimini cultivars significantly (P<0.05). The nitrajin and nitroxin treatments reduced calcium concentration, as compared to control, in the Rock feller cultivar.
Exogenous Eethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes
Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes

MA Nan,CAI Lei,LU Wangjin,TAN Hui,GAO Junping,
MA Nan
,CAI Lei,LU Wangjin,TAN Hui & GAO Junping .

中国科学C辑(英文版) , 2005,
Abstract: The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, 'Samantha', whose opening process is promoted, and 'Kardinal', whose opening process is inhibited by ethylene. Ethylene production and 1-aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in 'Samantha', and they were much more dramatically enhanced and peaked at the later stage (stage 4) in 'Kardinal' than control during vasing. cDNA fragments of three Rh-ACSs and one Rh-ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in 'Kardinal' than that of 'Samantha'. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhibited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in 'Kardinal'. Our results suggests that 'Kardinal' is more sensitive to ethylene than 'Samantha'; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in 'Samantha' and the inhibition in 'Kardinal'. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding
Influência de extratos vegetais no desenvolvimento in vitro de plantulas de Rosa x hybrida
Alves, Dejane Santos;Oliveira, Denilson Ferreira de;Pasqual, Moacir;Carvalho, Douglas Ant?nio de;Rodrigues, Vantuil A.;Carvalho, Daniel Diego Costa;
Ciência e Agrotecnologia , 2007, DOI: 10.1590/S1413-70542007000600044
Abstract: this work aimed to study the influence of plant extracts on the in vitro growth of rosa x hybrida plantlets, to contribute to the development of new plant growth regulators to propagate such ornamental plant. methanolic extracts of saintpaulia ionantha wendl. (leaves), hibiscus rosa-sinensis l. (leaves) and bougainvillea spectabilis wild. (leaves and flowers) were employed at 150 and 300 mg.l-1. flowering plants were used to obtain the extracts. the experiment was carried out in a randomized design, with ten treatments and four repetitions. culture medium with and without bap (6-benzylaminopurine) were used as controls. the analyzed variables were shoot number and length, root number and length, and leaves per shoot. regarding shoot number, all extracts were statistically identical to the control without bap. when root number and length were taken into account, only the extract of h. rosa-sinensis leaves at 300 mg.l-1 differed from the control without bap, with values statistically identical to the control with bap. except for the h. rosa-sinensis extract, all the others reduced the number of leaves per shoot. although the observed effects were not as pronounced as those obtained with bap, these results indicate the presence of plant growth regulators in the studied extracts.
Substrate pH level effects on anthocyanins and selected phenolics in Rosa x hybrida L. ‘KORcrisett’
Valentina Schmitzer, Franci tampar
Acta agriculturae Slovenica , 2010, DOI: 10.2478/v10014-010-0001-5
Abstract: The effect of substrate pH level (4.7, 3.3 and 7.3) on the anthocyanin, quercetin compounds, catechin and phenolic acids concentrations in petals of Rosa x hybrida L. ‘KORcrisett’ and on the number of flowers per plant was investigated. The phenolic profiles of this plant were established for the first time by the use of HPLC/MS. Plants potted in a substrate with pH 4.7 developed significantly more flowers compared to those planted in an acidic (3.3) and alkaline (7.3) pH levels. However, the concentration of anthocyanins, quercetin compounds, catechin and phenolic acids was always lowest in the petals of ‘KORcrisett’ rose plants potted in pH level 4.7. Compared to the first sampling, a significant increase in the concentration of major and total anthocyanins and quercetin compounds was measured in the petals of plants potted in pH level 3.3 and 7.3, but not in the plants potted in pH level 4.7, respectfully.
Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’)
Naphaporn Nak-Udom,Kantamaht Kanchanapoom,Kamnoon Kanchanapoom
Songklanakarin Journal of Science and Technology , 2010,
Abstract: A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’) was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS) containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.
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