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PENGEMBANGAN TEPUNG KAYA PROTEIN (TKP) dari KORO KOMAK (Lablab purpureus (L) Sweet) DAN KORO KRATOK (Phaseolus lunatus) [Development of Protein Rich Flour (PRF) from Hyacinth Bean (Lablab purpureus (L) Sweet) and Lima bean (Phaseolus lunatus)]  [cached]
Ahmad Nafi1),Tri Susanto2),Achmad Subagio1)
Jurnal Teknologi dan Industri Pangan , 2006,
Abstract: With respect to high content of carbohydrate and protein, Protein Rich Flour (PRF) were developed from non-oilseed legumes i.e. hyacinth bean (Lablab purpureus (L) Sweet) and lima bean (Phaseolus lunatus) PRF. PRFs were prepared using water and NaOH solution (0.01N) as extraction solvent. After precipitation in isoelectric point (pHs) the PRFs produced were characterized to determine the potential applications. The results showed that PRF from hyacinth bean which extracted by water was the best product with yield of 31.19%, protein content 58.41±4.45%, solubility 82-100% and oil holding capacity 93.92±9.19. Moreover pepsin-digestibility of the hyacinth bean PRF was higher (8.29±0.34%) than soybean protein isolate (7.10±0.37%) or casein (7.04±0.14%). Based on their characteristics, PRFs regarded as potential to be developed as novel food ingredient.
PENGARUH EKSTRAK PROTEIN KACANG KOMAK (Lablab purpureus (L.) Sweet) PADA KADAR GLUKOSA DAN PROFIL LIPIDA SERUM TIKUS DIABETES [Effect of Protein Extract of Hyacinth Bean (Lablab purpureus (L.) sweet) on Glucose and Lipid Profiles Serum In Diabetic Rats]  [cached]
Arif Hartoyo1)*,Deddy Muchtadi1),Made Astawan1),Dahrulsyah1)
Jurnal Teknologi dan Industri Pangan , 2011,
Abstract: The objectives of this research were to evaluate the effect of Lablab protein extract in diet on blood glucose concentration and lipid profiles of alloxan-induced diabetic rats. Two month old male Sprague Dawley rats were divided into 4 groups, containing 5 rats in each group. Three groups were diabetic rats induced by alloxan injection (110 mg/kg of body weight by intra-pheritonial injection) while one group was a normal rat as a control. The experimental groups were normal group, diabetic group, diabetic+cholesterol 0.5% group, and diabetic+cholesterol 0.5% + Lablab protein group. The concentration of rat’s blood glucose were periodically measured during diet intervenion (day 0,14,27, and 42). The result demonstrated that after 42 day of intervention, diet containing Lablab protein extract decreased the blood glucose concentration sharply from 455,75 mg/dl to 104.50 mg/dl (77%), while glucose concentration of control group only descreased from 458 mg/dl to 455.33 mg/dl (0.6%). Lablab protein diet significantly (p<0.05) reduce the concentration of blood glucose as compared to the control group. Lablab protein diet had a significant effect (p<0.05) on the reduction of serum total cholesterol, LDL cholesterol and triglycerides. Lablab protein diet seem to be benefecial for treating hyperglikemia and preventing diabetic complications.
PENGARUH FRAKSI KARBOHIDRAT KACANG KOMAK (Lablab purpureus (L.) sweet) TERHADAP KOLESTEROL DAN MALONALDEHID SERUM TIKUS PERCOBAAN YANG DIBERI RANSUM TINGGI KOLESTEROL [The Effect of Carbohydrate Fraction of Hyacinth Bean (Lablab purpureus (L.) sweet) on the Blood Serum Cholesterol and Malonaldehyde of Rats Fed with High Cholesterol Diet]  [cached]
Arif Hartoyo1),Dahrulsyah1),Nurheni Sripalupi1),Purwono Nugroho2)
Jurnal Teknologi dan Industri Pangan , 2008,
Abstract: The objective of this study was to analyze the effect of carbohydrate fraction of hyacinth bean (Lablab purpureus (L.) sweet) diet on lipid profile and peroxydation value of the blood serum and liver of rats. Fifteen male wistar strain rats were divided into three diet groups of five animals.The first group was positive control group with standard diet suplemented with 1 % pure cholesterol. The second group was negative control group with standard diet. The third group was given carbohydrate fraction of hyacinth bean and 1% pure cholesterol. The experiment was conducted for 75 days.The total cholesterol levels in positive, negative control, and that in the hyacinth treated group were 204.0, 74.8, and 99.8 mg/dl. The LDL levels in the control , negative control group, the hyacinth treated group were 153.0, 20.6, and 45.9 mg/dl. The HDL levels in positive control, negative control, and the hyacinth treated group were 26.7, 26.7, and 31.0 mg/dl. Triglyceride levels in the positive control, negative control, and the hyacinth treated group were 121.4, 137.7, and 114.8 mg/dl. The atherogenic index in the positive control, negative control, and the hyacinth treated group were 6.9, 1.9, and 2.5 point. The total cholesterol and LDL levels in the hyacinth treated group were significantly lower than those in the positive control which showed that the hyacinth treated group underwent hypercholesterolemia effect.Malonaldehyde content of the blood serum in positive control, negative control , and the hyacinth treated group were 0.54, 0.48, and 0.31 μmol/ml. Malonaldehyde content of liver organ in positive control group, negative control group, and the hyacinth treated group were 66.9, 36.4 and 73.4 nmol/g. Malonaldehyde content of blood serum in hyacinth treated group was significantly lower than that in the positive and negative control group. These results showed that the the hyacinth group decreased free radical in the blood serum. On the other hand, the malonaldehyde content of the liver in all treatment showed no significant differences.
PENGARUH FRAKSI NONPROTEIN KACANG KOMAK (Lablab purpureus (L.) Sweet) TERHADAP KADAR GLUKOSA DARAH DAN MALONALDEHIDA TIKUS DIABETES [Effect of Nonprotein Fraction of Hyacinth Bean (Lablab purpureus (L.) Sweet) Diet on Glucose and Malonaldehyde Serum of Diabetic Rats]
Arif Hartoyo*,Sukarno,Erma Rohmawati
Jurnal Teknologi dan Industri Pangan , 2010,
Abstract: The hypoglycemic response to Lablab nonprotein fraction (NPK) was evaluated in alloxan-induced diabetic rats. The objectives of this research were to evaluate the effect of Lablab nonprotein fraction diet on the blood glucose concentration and the lipid peroxide level of alloxan-induced diabetic rats. Two months old male Sprague Dawley rats were divided into 4 groups, each group contained of 5 rats. Three groups were diabetic rats induced by alloxan injection (110 mg/kg of body weight by intra-pheritonial injection) while one group was a control,normal rat. The experiment groups were (1) normal (group I), (2) diabetic (group II), (3) diabetic+cholesteol 0.5% (control group, group III), and (4) diabetic+cholesterol 0.5% + lablab NPK (group IV). The concentration of rat’s blood glucose were periodically measured during diet intervenion (day 0,14,27, and 42). The Lipid peroxide was evaluated as the concentration of malonaldehyde (MDA) both in serum and liver of the rats by Thiobarbituric Acid Reactivity Test methode. The result demonstrated that after 42 days of intervention, the Lablab nonprotein diet decreased the blood glucose concentrations from 444.00 + 143.00 mg/dl to 310.50 +111.40 mg/dl (30%), while control group has decreased the blood glucose concentration from 458.00 +164.99 mg/dl to 455.33 + 81.95 mg/dl (0.6%). Lablab nonprotein diet significantly (P<0.05) reduced the concentration of blood glucose as compared to the control group. However, Lablab nonprotein fraction diet did not give a significant diferrence on the level of serum MDA and liver MDA as compared to the control group.
Response of Proximate Composition of Lablab (Lablab purpureus L. Sweet) Herbage to Phosphorus Application, Cutting Height and Age of Cutting
S.A. Ogedegbe,V.B. Ogunlela,E.C. Odion,O.O. Olufajo
Research Journal of Agronomy , 2012, DOI: 10.3923/rjagr.2011.20.27
Abstract: In order to evaluate the response of lablab (Lablab purpureus L. Sweet) proximate composition to phosphorus application, cutting height and age of cutting, field trials were conducted at Samaru, Nigeria in the 2006-2008 wet seasons. The treatments were composed of factorial combinations of 4 rates of phosphorus application (0, 12, 24 and 36 kg P ha-1), 2 cutting heights (10 and 20 cm) as the main plot and 4 cutting ages (6, 12, 18 weeks and at maturity) as the sub-plot a split plot design with three replications. The highest crude protein and crude fibre concentrations of lablab herbage were obtained at the 12 weeks and maturity stages of cutting, respectively and both parameters were enhanced by advanced age of cutting. Lablab ether extract concentration decreased as age of cutting increased and cutting age did not improve ether extract concentration. A phosphorus application rate of 12 kg P ha-1 produced the highest ether extract concentration in lablab herbage when age of cutting was 6 weeks. Crude protein concentration of lablab was highest at the 0 P application rate combined with cutting at 6 weeks age while crude fibre was highest when lablab received no phosphorus application and was cut at maturity. It is suggested that lablab herbage be fed prior to maturity in order to reduce minimize crude protein loss.
Growth and Growth Attributes of Lablab (Lablab purpureus L. Sweet) as Influenced by Phosphorus Application, Cutting Height and Age of Cutting
S.A. Ogedegbe,V.B. Ogunlela,E.C. Odion,O.O. Olufajo
Research Journal of Agronomy , 2012, DOI: 10.3923/rjagr.2011.10.19
Abstract: With a view to studying the response growth and growth attributes of lablab (Lablab purpureus L. Sweet) to phosphorus application, cutting height and cutting age field trials were conducted during the 2006-2008 wet seasons at Samaru, Nigeria. The treatments were composed of factorial combinations of four rates of phosphorus application (0, 12, 24 and 36 kg P ha-1), two cutting heights (10 and 20 cm) and four ages of cutting (6, 12, 18 weeks and at maturity) in a split plot design with three replications. Application of 12 kg P ha-1 increased lablab sward height slightly (4%) while applying 24 kg P ha-1 increased leaf area index by 10%, number of root nodules by 42% and nodule dry weight by 50% over the zero-P control. Cutting lablab to a 20 cm stubble produced significantly taller sward. The highest sward obtained by cutting fodder at 18 weeks, a treatment that also produced the fastest relative regeneration rate. A combination of 36 kg P ha-1 and 20 cm cutting height produced the highest sward. The highest number of root nodules was obtained when lablab was given 24 kg P ha-1 and cut to a stubble height of 10 cm. Intensive management of lablab growth should be carried out within 12 WAS for the overall benefit of the crop. Cutting or grazing treatment either of which may prolong the vegetative stage of lablab is also required to manage its leaf area index for better crop growth.
Mineral Content of Lablab (Lablab purpureus L. Sweet) Herbage as Influenced by Phosphorus Application, Cutting Height and Age of Cutting at Samaru, Nigeria  [PDF]
S.A. Ogedegbe,V.B. Ogunlela,O.O. Olufajo,E.C. Odion
Asian Journal of Crop Science , 2012,
Abstract: As a result of the critical importance of herbage quality and danger of poor mineral nutrition of forage legumes that affect livestock feeds in Nigeria, this investigation was conducted. The experiment entailed field trials at Samaru, Nigeria over three wet seasons to evaluate the response of mineral composition of lablab (Lablab purpureus L. Sweet) herbage to phosphorus application and cutting treatments. The treatments were composed of factorial combinations of four rates of phosphorus application (0, 12, 24 and 36 kg P ha-1), two cutting heights (10 and 20 cm) as the main plot and four cutting ages (6, 12, 18 weeks and at maturity) as the sub-plot a in split plot design with three replications. Cutting lablab to a 10 cm stubble produced significantly higher calcium and phosphorus concentrations in the dry herbage than cutting to 20 cm. The Ca:P ratio in the herbage increased as age of cutting increased but cutting height did not influence this parameter. A phosphorus application rate of 12 kg P ha-1 produced the highest ash concentration in lablab herbage under a 12-week cutting regime. However, the highest phosphorus concentration of lablab herbage was produced with a zero phosphorus application rate under a cutting height of 10 cm. Under the first herbage cut, ash content increased significantly with age of cutting. It is beneficial to feed lablab herbage to livestock before crop attains physiological maturity. The nutritive value of the herbage determines the most appropriate time to feed lablab herbage to livestock.
Agronomic Performance and Sensory Evaluation of Lablab (Lablab purpureus L. Sweet) Accessions for Human Consumption in Uganda
Peace Kankwatsa, Robert Muzira
Open Access Library Journal (OALib Journal) , 2018, DOI: 10.4236/oalib.1104481
Abstract:
Lablab (Lablab purpureus) germplasm was evaluated to identify high grain yielding and palatable accessions that were suitable for human consumption in Uganda. A preference analysis was done to identify accessions that had a high probability of being accepted by farmers. Accessions 29399, 29400, 29803, 30701, 31364, CQ3620, Q5427, Q6988, 52518B, Q6880B, 31364, CQ3621 and Lablab Uganda had high yields, which partly resulted from their high tolerance of the prevailing stresses (diseases, pests and low soil moisture). Accessions 29400, Lablab Uganda, Njahi. 29399, 36019, Q5427, Q6988, 30701 and 31364 scored highly based on the sensory attributes. Accessions Lablab Uganda, Njahi, 29400 and Q69887 were the most accepted for adoption by farmers based on their high agronomic performance. Lablab Uganda, Njahi, 29400 and Q69887 had the most preferred palatability characteristics for human consumption.
ANTI-INFLAMMATORY, ANTIOXIDANT AND CYTOTOXICITY POTENTIAL OF METHANOLIC EXTRACT OF TWO BANGLADESHI BEAN LABLAB PURPUREUS (L.) SWEET WHITE AND PURPLE  [PDF]
Md. Razibul Habib et al.
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: This study was subjected to investigate in vitro anti-inflammatory, antioxidant and cytotoxic properties of methanol extracts of two Bangladeshi bean pods namely Lablab purpureus L. sweet ‘white’ and ‘purple’. Protease Inhibition method was used to determine in-vitro anti-inflammatory properties. DPPH free radical scavenging method, total flavonoids, Total antioxidants and reducing power of the two samples were assessed for antioxidant properties and cytotoxicity potentials were assessed by the brine shrimp lethality test. In in-vitro anti-inflammatory investigation there is a linear relation of %inhibition for the white bean pods which indicates having positive anti-inflammatory property. In DPPH test the lowest and highest IC50 values are 430.00μg/ml and 853.13μg/ml, with L. purpureus sweet ‘purple’ and L. purpureus sweet ‘white‘ respectively and compared with standard ascorbic acid. In Cytotoxicity test LC50 value was found 960.06 μg/ml for L. purpureus (L.) sweet ‘purple’ and 66.5 μg/ml for L. purpureus sweet ‘white’, So L. purpureus sweet ‘white’ is more potent. The total Flavonoid contents of the test samples are 42.55±5.77 and 32.09±0.36 mg/g quercetin equivalents for ‘white’ and ‘purple’ respectively. L. purpureus (L.) Sweet White and Purple may posses’ potent anti-inflammatory, powerful antioxidant as well as good cytotoxic potential.
Molecular Diversity of Kenyan Lablab Bean (Lablab purpureus (L.) Sweet) Accessions Using Amplified Fragment Length Polymorphism Markers  [PDF]
Esther N. Kimani, Francis N. Wachira, Miriam G. Kinyua
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.33037
Abstract: Lablab purpureus (L.) Sweet is a multipurpose legume that combines use as human food and animal feed in addition to serving as a cover crop for soil conservation. In this work, molecular diversity in Lablab purpureus was assessed using amplified fragment length polymorphism markers on fifty Kenyan lablab accessions obtained from farmers’ fields and the Kenya National gene bank. One hundred and eighty polymorphic bands were revealed using fifteen selective primer pairs. The overall mean expected heterozygosity (He) for the five populations was 0.189. Estimates of components of molecular variance revealed that most of the genetic variation resided within populations (99%) and only 1% variance was among the populations, while Principal Coordinate Analysis showed an overlap between accessions from different geographic origins. The UPGMA cluster analysis generated from the distance matrix of the 50 assayed accessions, revealed low diversity among most of the accessions. The low diversity observed may be due to the narrow genetic base for breeding stocks, and extensive exchange of germplasm among smallholder farmers across the country. Results obtained from this study are discussed in light of the need to enhance the genetic management and improvement of this multipurpose crop species.
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