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El rol de las bibliotecas digitales
López Guzmán, Clara
Técnica Administrativa , 2001,
Abstract: Descripción del rol de las bibliotecas digitales
Die kulturele rol van frankryk in Afrika  [cached]
A. A. F. Teurlinckz
Koers : Bulletin for Christian Scholarship , 1968, DOI: 10.4102/koers.v36i3.1298
Abstract: ,,Die kulturele rol van Frankryk in Afrika” was die onderwerp van die feesrede wat mnr. Joseph le Grand, algemene afgevaardigde van die Alliance frangaise in Suid-Afrika, gehou het ter geleentheid van die viering van die tiende bestaansjaar van die Cercle frangais de Potchefstroom.
Rol de género y funcionamiento familiar
Patricia Herrera Santi
Revista Cubana de Medicina General Integral , 2000,
Abstract: En este trabajo se realiza un breve bosquejo del surgimiento y significación del rol de género y se relaciona con algunos de los indicadores de funcionamiento familiar, analizando en qué medida puede afectar la expresión favorable del mismo, alterar la dinámica interna de las relaciones familiares y actuar como factor patógeno en la salud familiar A brief sketch of the appearance and significance of the gender role is made in this paper. It is also related to some indicators of family functioning and it is analyzed to what extent this relationship may affect its favorable expression, alter the internal dynamics of the family relations and act as a pathogenic factor in family health
Studies on Presence and Response of Agrobacterium rol Genes in Three Varieties of Tobacco  [PDF]
P. Vijay Joshua,D.R. Salomi Suneetha,A. Arundhati,G. Seshagiri Rao
Asian Journal of Plant Sciences , 2009,
Abstract: Present study was undertaken to identify the presence of rol genes of Agrobacterium rhizogenes in Nicotiana tabacum var. Samsun, N. undulata and N. tabacum var. Xanthi and their response towards hormonal shifts in culture. These three species of Nicotiana appear normal under field conditions and the leaf discs produced shoots on cytokinin containing Murashige and Skoog medium. These shoots when shifted to MS basal medium, N. tabacum var. Samsun showed rooty morphogenesis and the growth of the shoots continued for N. undulata but the growth stopped and the discs turned brown in N. tabacum var. Xanthi. Similar results with rooty morphogenesis were observed on MS supplemented with auxin medium. Genomic DNA of these three Nicotiana species when used for PCR analysis with rol A, B, C and D gene primers of A. rhizogenes, N. tabacum var. Samsun showed amplification of rol A, B, C and D genes, N. undulata showed amplification of rol A and C genes and no specific amplification was observed for N. tabacum var. Xanthi. The results were discussed in terms of the correlation between the presence of different combination of rol genes in N. tabacum var. Samsun and N. undulata and their response for habituation on hormone free medium with differential morphogenesis on hormonal shifts.
EL ROL DEL FONOAUDIóLOGO EN UN HOSPITAL MATERNO INFANTIL  [cached]
Gabriela Allignani,Gustavo Granovsky,Marisa Iroz
Revista del Hospital Materno Infantil Ramón Sardá , 2010,
Abstract: Objetivo: informar y difundir las tareas profesionales que desempe a el fonoaudiólogo, su inclusión dentro del ámbito de la atención primaria de la salud, la actividad interdisciplinaria que desarrolla y su rol en el diagnóstico precoz.
Consecuencias del estrés de rol Consequences of role stress  [cached]
Fernando Mansilla Izquierdo
Medicina y Seguridad del Trabajo , 2011,
Abstract: El estrés de rol es específicamente el que se origina por el desempe o de roles en la organización, y comprende tanto la ambigüedad de rol como el conflicto de rol. Objetivo: Evaluar la ambigüedad y el conflicto de rol de un grupo de profesionales que realizan diferentes puestos de trabajo, y su influencia sobre sus niveles de bienestar físico y psicológico. Material y método: Para evaluar los estresores de rol (Ambigüedad de Rol y Conflicto de Rol) se ha utilizado el Cuestionario de Rizzo, House y Lirtzman. Y para establecer las consecuencias el Cuestionario de Síntomas SLC-90-R. Resultados: El 20% de la muestra percibía ambigüedad y conflicto de rol en su trabajo. Y formaron el grupo que puntuó alto en ambigüedad y conflicto de rol puntuó asimismo alto en todas las subescalas: Somatización, de Obsesión-Compulsión, de Sensibilidad Interpersonal, Depresión, Ansiedad, Hostilidad, Ansiedad Fóbica, Ideación Paranoide y de Psicoticismo. Además el índice Global de Gravedad y el Total de Síntomas Positivos fueron significativamente superiores en el grupo que puntuó alto en ambigüedad de rol y conflicto de rol. Por otro lado, no se encuentran diferencias significativas entre los niveles de estudios ni en las subescalas ni en índice General de Gravedad ni en el Total de Síntomas Positivos. The role stress is specifically the one that originates from the performance of roles in the company, and includes both role ambiguity and role conflict. Objetive: To evaluate the ambiguity and the role conflict of a group of professionals who carry out different jobs, and its influence on their physical and psychological well-being. Material and method: To evaluate the role stressors (Role Ambiguity and Role Conflict) the Questionnaire of Rizzo, House and Lirtzman has been used and to establish the consequences the Questionnaire of Symptoms SLC-90-R. Results: 20 % of the sample percieved ambiguity and conflict of role in their job and had high punctuation in all the subscales: Somatization, Obsessive- Compulsive, Interpersonal Sensibility, Depression, Anxiety, Hostility and Phobic Anxiety. In addition, the Global Rate of Gravity and the Total Positive Symptoms were significantly higher in the group that had high punctuation in ambiguity of role and conflict of role. On the other hand, we did not find significant differences in the level of studies in the subscales ,nor the General Rate of Gravity nor in the Total Positive Symptoms.
发根农杆菌Ri质粒rol基因研究的综述 Review of Studies on Rol Genes of Ri Plasmids from Agrobacterium rhizogenes
周跃钢,王三根ZHOU Yue-Gang,WANG San-Gen
遗传 , 1997,
Abstract: 发根农杆菌Ri质粒rol基因研究的综述①周跃钢王三根(西南农业大学生理生化教研室,重庆400716)ReviewofStudiesonRolGenesofRiPlasmidsfromAgrobacteriumrhizogenesZhouYuegang...
Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris
米根霉脂肪酶基因pro-ROL和m-ROL在毕赤酵母中的密码子优化、表达和酶学性质的比较分析

Jiangke Yang,Xiangxiang Yan,Xiangxiang Yan,Bo Zhang,
杨江科
,严翔翔,黄日波,张搏

生物工程学报 , 2011,
Abstract: Rhizopus oryzae lipase (ROL) is not only a biocatalyst used in a broad range of biotechnological fields, but also a model to investigate the function of intramolecular chaperone in the post-translational processing of lipase. In this study, we cloned and expressed the mature lipase gene (m-ROL) containing the pre-sequence (pro-ROL) of R. oryzae HU3005 in Pichia pastoris GS115 and characterized their enzymatic activities. m-ROL exhibited higher hydrolysis activity towards middle-chain substrates (C10 and C12) at pH 9.0, whereas pro-ROL preferred short-chain substrates (C4) and displayed maximal activity at pH 8.0. Moreover, pro-ROL possessed better thermal stability than m-ROL. This enzymatic discrepancy between m-ROL and p-ROL may be due to the pre-sequence that affects the folding and conformation of the mature lipase domain. To improve the expression level of m-ROL in P. pastoris, overlap extension PCR was conducted to substitute eight less-frequently used codons of m-ROL with frequently used codons. After methanol-induced expression for 72 h, the activity and protein content of the codon optimized m-ROL reached 132.7 U/mL and 50.4 mg/L, while the activity of the parental m-ROL and pro-ROL are 28.7 U/mL and 14.4 mg/L, 29.6 U/mL and 14.1 mg/L, respectively.
El rol de la usabilidad percibida sobre la confianza en las subastas online
Torres-Moraga, Eduardo;Barra, Cristobal;
Revista de Administra??o de Empresas , 2011, DOI: 10.1590/S0034-75902011000500006
Abstract: usability has been considered as a key variable that allows trust development in online environments. however, the relationship between these concepts has not been studied in depth to realize if the effect is direct or mediated. in this context, it is proposed that the perceived usability affects trust not in a direct way but considering the role of satisfaction as a mediator. this study considers the specific context of internet auctions that represents a growing services market through internet in latin america. after testing the unidimensionality, reliability and validity of the measurement scales, hypotheses were tested using a structural equation modeling, a rival models strategy and a multi-sample analysis.
EL ROL DE LA USABILIDAD PERCIBIDA SOBRE LA CONFIANZA EN LAS SUBASTAS ONLINE  [PDF]
Eduardo Torres-Moraga,Cristobal Barra
Revista de Administra??o de Empresas , 2011,
Abstract: Usability has been considered as a key variable that allows trust development in online environments. However, the relationship between these concepts has not been studied in depth to realize if the effect is direct or mediated. In this context, it is proposed that the perceived usability affects trust not in a direct way but considering the role of satisfaction as a mediator. This study considers the specific context of Internet auctions that represents a growing services market through Internet in Latin America. After testing the unidimensionality, reliability and validity of the measurement scales, hypotheses were tested using a structural equation modeling, a rival models strategy and a multi-sample analysis
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