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Chemical Constituents of the Essential Oil of Stachys lavandulifolia Vahl from Iran
Katayoun Javidnia,Faraz Mojab,Seyed Ali Mojahedi
Iranian Journal of Pharmaceutical Research , 2004,
Abstract: The essential oil of Stachys lavandulifolia Vahl (Lamiaceae) was isolated by of the aerial parts of the plant, with a yield of 0.25%. The chemical composition of volatile oil was analyzed by capillary GC and GC/ MS. The main components were germacrene-D (13.2%), β-phellandrene (12.7%), β-pinene (10.2%), myrcene (9.4%), α-pinene (8.4%) and Z-β-ocimene (5.8%).
Anti-Oxidative Stress Activity of Stachys lavandulifolia Aqueous Extract in Human  [PDF]
Kobra Rahzani,Ali Akbar Malekirad,Akbar Zeraatpishe,Naser Hosseini
Cell Journal , 2013,
Abstract: Medicinal plants are presumed to be natural sources of antioxidants that protect organisms from oxidative stresses. The present investigation aims to study the anti-oxidative stress activity of the Stachys lavandulifolia (S. lavandulifolia) plant. This trial was conducted on 26 healthy human subjects. The study was done in a before after fashion. The included subjects were asked to consume the prepared infusion from 3 g aerial parts of S. lavandulifolia on a daily basis. Doses were administered in every morning and evening for 14 days. At the beginning and the end of the study, blood samples were acquired to determine the level of cellular lipid peroxidation and the total content of serum antioxidants. Biomarkers analyzed from samples obtained before start of treatment and 14 days post treatment, were subjected to paired t test analysis. Total blood antioxidants increased and reached from 2.3 ± 0.84 μmol/ml to 3.3 ± 0.54 μmol/ml. The lipid peroxidation reduced and reached from 8.38 ± 1.78 to 11.6 ± 2.64 nmol/ml. The results suggest that S. lavandulifolia possesses marked anti-oxidative stress activity and it can be useful as a supplement in the management of diseases related to oxidative stress (Registration Number: IRCT2013012210003N2).
Wound Healing Activity of Extracts of Malva sylvestris and Stachys lavandulifolia  [cached]
Abdollah Ghasemi Pirbalouti,Abed Koohpyeh
International Journal of Biology , 2010, DOI: 10.5539/ijb.v3n1p174
Abstract: The flowers of Stachys lavandulifolia Vahl (Lamiaceae) and Malva sylvestris Linn (Malvaceae) are traditionally used to treat various skin disorders, antimicrobial and anti-inflammatory. The chloroform extract of M. sylvestris flowers and aqueous extract of S. lavandulifolia flowers were used to evaluate the wound healing activity at 200 mg/kg/day dose. Wounds were induced in Wistar rats divided into four groups as following; Group-I was treated with cold cream. Groups-II and -III were treated with cold cream containing of extracts, Group-IV received the standard drug (Nitrofurazone). The efficacy of treatment was evaluated based on wound area and histopathological characteristics. The extract-treated animals by M. sylvestris showed significant reduction in the wound area when compared with other groups. Also, histopathological studies of the tissue obtained on days 6th, 9th and 16th from the extract-treated by M. sylvestris showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells.
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
Faezeh Monji,Hiva Hossein Tehrani,Zahra Halvaei,Sepideh Arbabi Bidgoli
Acta Medica Iranica , 2011,
Abstract: Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg kg. Doses up 70 mg kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant.
Polyphenol Contents and Antioxidant Properties of Medlar (Mespilus germanica L.)  [PDF]
?lhami Gül?in,Fevzi Topal,S. Beyza ?ztürk Sar?kaya,Ercan Bursal
Records of Natural Products , 2011,
Abstract: Medlar is the fruit of Mespilus germanica L. in the family of Rosaceae. The present study outlines that the native medlar (Mespilus germanica L.) fruits an extremely rich source of antioxidants. In this study, antioxidant and antiradical property of medlar fruits were evaluated. Total phenolics and flavonoids amounts in lyophilized extract of medlar (LEM) fruits were calculated as gallic acid and quercetin equivalents, respectively. Antioxidant and radical scavenging activity of LEM were investigated using different in vitro assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH ), N,N-dimethyl-p-phenylenediamine (DMPD +), and superoxide anion radicals (O 2 -) scavenging activity, hydrogen peroxide (H 2O 2), ferric ions (Fe 3+) and cupric ions (Cu 2+) reducing ability, Fe 3+-TPTZ reducing ability, ferrous ions (Fe 2+) chelating activity as trolox equivalent. In addition, quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid in LEM were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). The presence of these antioxidant compounds can be considered as a quality parameter for edible medlar fruits.
Chemical Constituents of the Essential Oils of Different Stages of the Growth of Stachys lavandulifolia Vahl. From Iran
Mohammad Hadi Meshkatalsadat,Esmat Sajjadi,Hamzeh Amiri
Pakistan Journal of Biological Sciences , 2007,
Abstract: The essential oil of the aerial parts of different stages of growth as pre-flowering, flowering and post flowering of Stachys lavandulifolia Vahl (Lamiaceae) were isolated by hydro distillation. The chemical composition of volatile oil was analyzed by capillary GC and GC/MS. The main components were found to be: α-pinene) 27.25, 25.66, 8.52%), myrcene (17.33, 9.33, 23.85%), β-phellandrene (21.96, 37.49, 12.58%), β-caryophylene (14.3, 8.38, 16.86%).
Effect of Five Different Stages of Ripening on Chemical Compounds in Medlar (Mespilus germanica L.)  [PDF]
Otakar Rop,Jiri Sochor,Tunde Jurikova,Ondrej Zitka,Helena Skutkova,Jiri Mlcek,Petr Salas,Boris Krska,Petr Babula,Vojtech Adam,Daniela Kramarova,Miroslava Beklova,Ivo Provaznik,Rene Kizek
Molecules , 2011, DOI: 10.3390/molecules16010074
Abstract: The study of changes of nutritional value of fruit during the ripening process can help estimate the optimal date for fruit harvesting to achieve the best quality for direct consumption and further utilization. The aim of this study was to monitor the changes of chemical composition of medlar fruit (Mespilus germanica L.) measured at five various ripening stages including 134, 144, 154, 164 and 174 days after full bloom (DAFB). Fruits were analyzed and ascorbic acid (AA) and total phenolic compound content with respect to the total antioxidant activity were determined. In addition, selected micronutrients and macronutrients were monitored. The results of our experiments demonstrate that ascorbic acid, total phenolic compound content and total antioxidant activity decreased significantly with increasing time of ripeness. The decreasing tendency in potassium, calcium and magnesium contents during the ripening stages was also determined. During the ripening period, the content of all micronutrients as well as phosphorus and sodium was balanced, with no statistically significant differences between the monitored ripening stages, which can be considered as a positive fact with respect to ideal consumption quality of fruit.
Evolution of fatty acids in medlar (Mespilus germanica L.) mesocarp at different stages of ripening
Ayaz, F. A.,Glew, R. H.,Huang, H. S.,Chuang, L. T.
Grasas y Aceites , 2002,
Abstract: The fatty acid composition of medlar (Mespilus germanica L.) varied significantly among the ripening stages sampled at 157, 172 and 187 DAFs (days after full bloom). Twenty-one different fatty acids were detected in preclimacteric fruit and 17 when the climacteric began. Principal fatty acids, determined in medlar fruit harvested from October (157 and 172 DAFs) to November (187 DAF) were mainly palmitic acid (16:0), linoleic acid (18:2n-6), and a-linolenic acid (18:3n-3). While the content of saturated fatty acids [palmitic acid (16:0) and stearic acid (18:0)] increased, the content of the essential polyunsaturated fatty acids [linoleic acid (18:2n-6) and linolenic acid (18:3n-3)] decreased through ripening, in parallel with pulp darkening. The percentage of linoleic acid and a-linolenic acid in ripe, hard fruits was 60.0 and 13.5 % of dry wt at 157 DAF which decreased throughout ripening, remaining at 28.7 and 5.6 % of dry wt, respectively, in the fully softened and darkened pulp. A marked decreases in the double bond index, percentage of unsaturation and the ratio of unsaturation/saturation were also seen throughout the medlar ripening. The contribution of unsaturated fatty acid to the total fatty acid content decreased markedly as the medlar fruit became progressively softer and darkened. La composición en ácidos grasos del níspero (Mespilus germanica L.) varió significativamente entre los estados de maduración muestreados a los 157, 172 y 187 DAFs (días después de la floración). Veinte y un ácidos grasos diferentes fueron detectados en el fruto preclimatérico y 17 cuando comenzó el climaterio. Los ácidos grasos principales encontrados en nísperos, recolectados desde Octubre (157 y 172 DAFs) hasta Noviembre (187 DAF), fueron principalmente ácido palmítico (16:0), ácido linoléico (18:2n-6), y ácido a-linolénico (18:3n-3). En tanto que el contenido en ácidos grasos saturados (ácido palmítico (16:0) y ácido esteárico (18:0)) aumentó, el contenido en ácidos grasos esenciales (ácido linoleico (18:2n-6) y ácido linolénico (18:3n-6)) disminuyó durante la maduración, en paralelo con el oscurecimiento de la pulpa. El porcentaje de ácido linoleico y de ácido a-linolénico en frutos maduros sin reblandecer fue de 60.0 y 13.5 % del peso seco a 157 DAF, disminuyendo durante la maduración, y permaneciendo a 28.7 y 56 % del peso seco, respectivamente, en la pulpa completamente blanda y oscura. También se observó durante la maduración del níspero una marcada disminución en el número de dobles enlaces, en el tanto por ciento de instauración y en la relación instauraci
Antioxidant activity of the methanolic extracts of some species of Phlomis and Stachys on sunflower oil
K Morteza-Semnani, M Saeedi, S Shahani
African Journal of Biotechnology , 2006,
Abstract: Antioxidant effects of the methanolic extract of Phlomis bruguieri, P. herba-venti, P. olivieri, Stachys byzantina, S. inflata, S. lavandulifolia and S. laxa were tested in sunflower oil stored at 70oC, by measuring peroxide values after regular intervals and compared with rosemary-, green tea- and BHAcontaining samples. The methanolic extracts of P. bruguieri and S. laxa were found to be most effective in stabilizing sunflower oil.
Antimicrobial studies on extracts of four species of Stachys  [cached]
Saeedi M,Morteza-Semnani K,Mahdavi M,Rahimi F
Indian Journal of Pharmaceutical Sciences , 2008,
Abstract: The antimicrobial activity of the methanol extracts of the dried flowering aerial parts of Stachys byzantina , S. inflata , S. lavandulifolia and S. laxa (Labiatae) were studied using the disc diffusion method and determination of minimum inhibitory concentration (MIC) values against Staphylococcus aureus , Streptococcus sanguis , Escherichia coli , Pseudomonas aeroginosa , Klebsiella pneumoniae , Aspergilus niger and Candida albicans . The extracts of plants exhibited concentration-dependent antibacterial activity against the bacteria tested. The extracts were more active against Gram-positive microorganisms. The extracts, however, did not show any antifungal activity.
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