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The pathogens of white mildew disease of cultivated Auricularia auricula-judae in Northeast China
东北地区木耳“白毛菌病”的病原菌

KONG Xiang-Hui,LIU Jia-Ning,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hu,MA Qing-Fang,ZHANG Jie-Chi,
孔祥辉
,刘佳宁,张丕奇,戴肖东,韩增华,马庆芳,张介驰

菌物学报 , 2011,
Abstract: The white mildew disease on Auricularia auricula-judae was epidemic in recent years in Northeast China. The pathogenic fungi formed a layer of white pull-mesh mycelium on the ventral side of the fruiting body of A. auricula-judae in hot summer. The fungi significantly reduce the yield as well as the quality of the fruiting bodies. In this study, two fungal strains were isolated from the infected fruiting bodies cultivated in Shangzhi of Heilongjiang Province. They were identified as Fusarium oxysporum and Fusarium chlamydosporum. Both of them are pathogenic to the fruiting body of A. auricula-judae.
The physiological characteristics and genetic diversity analysis of Auricularia auricula-judae cultivated germplasm in China
中国木耳栽培种质生物学特性及遗传多样性分析

LI Li,FAN Xiu-Zhi,XIAO Yang,ZHOU Yan,BIAN Yin-Bing,
李黎
,范秀芝,肖扬,周雁,边银丙

菌物学报 , 2010,
Abstract: The physiological characteristics of 32 main cultivated strains of Auricularia auricula-judae in China were analyzed, and the 27 indexes were evaluated and clustered by UPGMA and PCO. The results demonstrated that the cultivated germplasm of A. auricula-judae in China are richly endowed with genetic diversity, and the 32 strains analyzed are different physiologically. The strains can be divided into 3 groups, group of strains mainly cultivated in northeast region, group of strains in central and southeast regions, and group of strains in north, south and part of central regions. It is revealed that the physiological characteristics significantly correlated with geographical origins. The cultivated strains in the same region are more similar physiologically and some strains are suspected to be synonymous.
PHYLOGENETIC RELATIONSHIPS AND REVIEW OF THE SPECIES OF AURICULARIA (FUNGI: BASIDIOMYCETES) IN COLOMBIA
MONTOYA-ALVAREZ,ANDRéS FELIPE; HAYAKAWA,HIROSHI; MINAMYA,YUKIO; FUKUDA,TATSUYA; LóPEZ-QUINTERO,CARLOS ALBERTO; FRANCO-MOLANO,ANA ESPERANZA;
Caldasia , 2011,
Abstract: the phylogenetic relationship of the species of the genus auricularia and its allied taxa were investigated using the internal transcribed spacer (its) sequences of nuclear dna. a molecular phylogenetic tree constructed using a total of 17 samples representing five species and two outgroups indicate that the species of auricularia form a monophyletic group. within the genus auricularia, a. mesenterica is basal and the remaining auricularia species form three clades; first clade consisted of a. auricula-judae; the second clade of a. fuscosuccinea, and the third clade of a. polytricha.
Liquid-state fermentation with Bacillus subtilis Bs-07 to enhanceanticoagulant function of Auricularia auricula polysaccharide  [PDF]
Zhang Zhi, Liu Dongmin, Xu Bingxin, Hu Junfei, Liu QiGuo Yong, Liang Zhuoran, Lu Xiangni, Zhao Zichen, Wang Zhenyu
- , 2015, DOI: 10.3969/j.issn.1003-7985.2015.01.024
Abstract: In order to improve the anticoagulant function of Auricularia auricula, Auricularia auricula polysaccharide(AAP)was converted into its derivatives by the microbial fermentation method and then polysaccharide derivatives with stronger anticoagulant activity were prepared. The optimal conditions for fermenting the polysaccharide from A. auricula were examined and the in vitro anticoagulant activities of transformed and untransformed polysaccharides were compared. Response surface tests and an orthogonal experiment indicated that the best conditions for microbial conversion of AAP3 were an AAP3 concentration of 4.0 mg/mL, a ratio of substrate(AAP3)to donor(p-hydroxybenzoic acid)of 40∶1, and a pH of 6.0. Bacillus subtilis Bs-07 was inoculated and then placed on a rotary shaker(120 r/min), followed by fermentation for 48 h at 35 ℃.The conversion rate was found to be greater than 40%. The result of in vitro anticoagulant activity showed that the transformed polysaccharide improved activated partial thromboplastin time, prothrombin time, and thrombin time values and greatly enhanced anticoagulant activity compared to the untransformed polysaccharide.
Identification of cultivated strains of Auricularia auricula from northeastern China by ISSR marker
用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究

ZHANG Jie-Chi,MA Qing-Fang,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hua,KONG Xiang-Hui,
张介驰
,马庆芳,张丕奇,戴肖东,韩增华,孔祥辉

微生物学报 , 2007,
Abstract: Inter simple sequence repeat (ISSR) method was used to distinguish the strains of Auricularia auricula in northeastern China. The result showed that 10 primers selected from 20 ISSR primers tested could produce distinct and polymorphic fingerprints of 27 cultivated strains of A. auricula. The result from the clustering analysis by NTSYS showed that these cultivated strains could be divided into three groups at similar level of 0.75. The result indicated that ISSR marker was an effective tool for strain identification and fingerprint analysis of A.auricula.
Identification of cultivated strains of Auricularia auricula from northeastern China by ISSR marker
用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究

ZHANG Jie-Chi,MA Qing-Fang,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hua,KONG Xiang-Hui,
张介驰
,马庆芳,张丕奇,戴肖东,韩增华,孔祥辉

菌物学报 , 2007,
Abstract: Inter simple sequence repeat (ISSR) method was used to distinguish the strains of Auricularia auricula in northeastern China. The result showed that 10 primers selected from 20 ISSR primers tested could produce distinct and polymorphic fingerprints of 27 cultivated strains of A. auricula. The result from the clustering analysis by NTSYS showed that these cultivated strains could be divided into three groups at similar level of 0.75. The result indicated that ISSR marker was an effective tool for strain identification and fingerprint analysis of A.auricula.
Suitability of Selected Supplemented Substrates for Cultivation of Kenyan Native Wood Ear Mushrooms (Auricularia auricula)  [PDF]
B.O. Onyango,V.A. Palapala,P.F. Arama,S.O. Wagai
American Journal of Food Technology , 2011,
Abstract: Different organic substrates namely maize cobs, wheat straw, grass straw and sugarcane bagasse supplemented with either wheat or rice bran were evaluated for production of two Kenyan native strains of wood ear mushroom [Auricularia auricula (L. ex Hook.) Underw.]. The objective was to evaluate the suitability of these substrates for cultivation of Kenyan native wood ear mushroom. Plastic bag technology was used with treatments arranged in a completely randomized design replicated three times. Samples of black and brown strains of the wood ear mushroom collected from woody stems of dead and dying trees within Kakamega forest were used in this study. Data was collected on days to pinning, fruit body quality, fruit body yields (number and fresh weight) and biological efficiency. The data collected was subjected to analysis of variance using SAS version 9.1. Mean separation was done using LSD and effects declared significant at 5% level. The two mushroom strains were not significantly (p>0.05) different in performance except for the number of fruit bodies where the black strain yielded significantly (p<0.05) higher than the brown one. The best performance was obtained from maize cobs and wheat straw substrates supplemented with wheat bran and these combinations were recommended to wood ear mushroom growers.
Chemical structure of polysaccharide AAPS-3 from Auricularia auricula-judae
黑木耳多糖AAPS-3的化学结构

SONG Guang-Lei,DU Qi-Zhen,
宋广磊
,杜琪珍

菌物学报 , 2010,
Abstract: 利用逆流色谱分离技术从黑木耳中分离了黑木耳多糖AAPS-3。通过完全水解、甲基化、一维和二维1H和13C 核磁共振(NMR)阐明AAPS-3的化学结构。经完全水解证实AAPS-3主要由Glc单糖组成,用HPLC方法测得其分子量为4.83×105,通过甲基化反应数据和1H和13C NMR等分析发现AAPS-3为由β-D-1,4-Glc,β-D-1,3-Glc和β-D-1,6-Glc残基构成主链的葡聚糖。
ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China
中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记

TANG Li-Hu,XIAO Yang,BIAN Yin-Bing,
唐利华
,肖扬,边银丙

微生物学报 , 2008,
Abstract: Inter-simple sequence repeat(ISSR) was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region (SCAR) markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains of A. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula is practicable and significative.
ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China
中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记

TANG Li-Hu,XIAO Yang,BIAN Yin-Bing,
唐利华
,肖扬,边银丙

菌物学报 , 2008,
Abstract: Inter-simple sequence repeat(ISSR) was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region (SCAR) markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains of A. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula is practicable and significative.
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