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Selenium-enriched fermentation mycelia of Coprinus comatus enhance the antihyper- glycemia and antioxidant activities in alloxan-induced diabetic mice
富硒发酵毛头鬼伞菌丝对四氧嘧啶致糖尿病小鼠抗氧化和降血糖的影响

YU Jie,CUI Peng-Ju CHEN Mei-Zhen ZHONG Ming-Qi CUI Shi-Chao,WU Qing-Yang,ZHUO Jian-Yong,
余杰
,崔鹏举 陈美珍 钟名其 崔仕超 吴清洋 卓健勇

菌物学报 , 2009,
Abstract: This study evaluated the effect of selenium-enriched fermentation mycelia of C. comatus (SMCC) on reducing oxidative stress and hyperglycemia in alloxan-induced diabetic mice. After induction of diabetes, a significant increase of the levels of blood glucose and lipid peroxidation associated with the diminution of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) was observed in all diabetic mice. Treatment of diabetic mice with SMCC for 21 days resulted in a significant reduction in blood glucose and the injuries of pancreas. In addition, a significant increase in the activities of SOD and GSH-Px and a significant decrease in the content of malondialdehyde (MDA) were observed in serum, liver and kidney. The results showed that SMCC might be able to inhibit hyperglycemia, and helpful in the prevention of diabetic complications associated with oxidative damage. It is concluded that SMCC possesses potent antioxidant activity, which may be directly or indirectly responsible for inhibiting hyperglycemia of alloxan-induced diabetic mice.
Some morphological and physiological aspects and cultivation of Coprinus comatus (O. F. Mull.) Gray
Chaiyama, V,Petcharat, V.,Kritsaneepaiboon, P.
Songklanakarin Journal of Science and Technology , 2007,
Abstract: Some morphological and physiological aspects and cultivation of Coprinus comatus (O.F. Mull.) Gray were investigated. Malt extract agar turned out to be the best in supporting the mycelial growth of C.comatus. Mannose and maltose were the best carbon sources in supporting mycelial growth. C. comatus utilized peptone and NH4NO3 better than other nitrogen sources. The optimum temperature and pH on maltextract agar were 25oC and 6, respectively. Light retarded mycelial growth of C. comatus. For cultivation, the method of growing mushroom in autoclavable plastic bags was applied. Three different combinations of agricultural products were used for growing C. comatus. The combination ofpararubber sawdust : kapok waste : boiled sorghum seeds (3:3:1 by volume) supported higher yields of basidiocarps. Time required for full colonization of the mycelia on 500 gm substrate at room temperature(28-30oC) was 20.3 days. After casing with the mixed soil (loam soil : rice husk : cow manure, 2:2:1) the cultivating bags were incubated in the growth chamber at 20oC and 65% relative humidity. Fructificationbegan after 20.6 days of watering, and the average yield obtained was 165.6 g/bag.
Anti-Oxidative Activity of An Aqueous Suspension of Commercial Preparation of The Mushroom Coprinus comatus  [PDF]
Mira Popovi?,Sa?a Vukmirovi?,Neboj?a Stilinovi?,Ivan ?apo,Vida Jakovljevi?
Molecules , 2010, DOI: 10.3390/molecules15074564
Abstract: In this study the effects of an aqueous suspension of a commercial preparation of the mushroom Coprinus comatus on oxidative stress induced in rats by alloxane and carbon tetrachloride was examined. The effects were estimated from changes in the biochemical parameters (xanthine oxidase, glutathione peroxidase and catalase activity, reduced glutathione content, and extent of lipid peroxidation) of liver homogenate as well as histological changes in the liver of the rats treated with alloxane and carbon tetrachloride. Two screening doses of alloxane sufficient to induce diabetes in rats did not have any significant effect on the examined biochemical parameters of liver homogenate or on the cytoarchitectonics of liver cross-sections. Treatment with carbon tetrachloride resulted in a significant increase in the intensity of lipid peroxidation and peroxydasis activity, as well as with decrease in catalase activity. Certain changes in liver cross sections were detected, such is lymphocyte infiltration of dilated sinusoid capillaries. Administration of Coprinus comatus suspension thus showed antioxidative potential, evidenced by an increase of antioxidative status of liver homogenate and prevention of histological changes in liver cross sections.
Isolation and biological activity of triglycerides of the fermented mushroom of Coprinus Comatus
Jun Ren, Jin-Li Shi, chunchao han, Zhen-Quan Liu, jianyou guo
BMC Complementary and Alternative Medicine , 2012, DOI: 10.1186/1472-6882-12-52
Abstract: The effects of TFC on cytokines levels, total antioxidant activity, antinociceptive effects in vivo, LD50 and tactile hyperalgesia were analyzed respectively.TFC treatment decreased the levels of cytokines and total antioxidant status (TAOS) and inhibited the acetic acid-induced abdominal constrictions in mice. In addition, TFC reduced CFA-induced tactile hyperalgesia in a dose-dependent manner and the LD50 of TFC was determined to be 400?mg/kg. However, TFC did not significantly inhibit the reaction time to thermal stimuli in the hot-plate test.TFC showed anti-inflammatory, antioxidant, peripheral antinociceptive and antihyperalgesic activity in various models of inflammatory pain. The data suggest that TFC may be a viable treatment option for inflammatory pain.
Production of ligninase by co-fermentation of Coprinus comatus and Trichoderma reesei
里氏木霉和鸡腿菇利用秸秆共发酵产木质降解酶

Chunmei Ge,Juanjuan Xu,Qinying Sun,Jie Zhang,Jingmin Cai,Renrui Pan,
葛春梅
,徐娟娟,孙芹英,张洁,蔡敬民,潘仁瑞

生物工程学报 , 2009,
Abstract: In order to enhance the utilization efficiency,reduce the environmental pollution of traditional chemical treatment and the agriculture waste incineration;we studied the ligninase production by Coprinus comatus,Aspergillus niger and Trichoderma reesei through the plate screening.The results showed that C.comatus mixed culture with T.reesei have a good compatibility and higher yields of Laccase.On the basis of this pre-experiment,we studied the optimal conditions of mixed culture for enzyme production.Under ...
DEGRADATION AND TRANSFORMATION OF COTTON SEED HULLS BY COPRINUS COMATUS
鸡腿菇对棉籽壳的降解与转化

NI Xin-jiang,FENG Zhi-Yong,LIANG LI-Kun,YOU Cui-Rong,PAN Ying-Jie,
倪新江
,梁丽琨,由翠荣,冯志勇,潘迎捷

微生物学通报 , 2002,
Abstract: Coprinus comatus cultivated on cotton seed hull medium decomposed lignocellulose straggly and was high of absolute biological efficiency. Lignocellulose is the main carbon source for the fruiting stage of the fungus. There existed the positive correlation between the degradation rates of the cellulose and hemicellulose in the medium and the activities of extracellular CMCase (carboxymenthelcel lulase), FPase (filter paper cellulase) and HCase (hemicellu-lase), there also existed the positive correlation between the degradation rate of the lignin in the medium and the activity of extracellular laccase, but no correlation between the degradatio rate of the lignin in the medium and the activity of peroxidase. The activity of extracellular amylase was comparatively high at mycelial growth stage, and the protease activity peek was at teh time when the fruitbody matured.
毛头鬼伞(Coprinus comatus)中一种碱性蛋白的纯化及其活性
吴丽萍,吴祖建,林奇英,谢联辉
微生物学报 , 2003,
Abstract: 用离子交换层析(CMsepharose FF)和凝胶层析(SuperdexTM75)方法,从新鲜食用菌毛头鬼伞(Coprinus comatus)子实体中分离纯化出一碱性蛋白y3,经SDSPAGE初步确定其分子量约为14.4kD。活性检测结果显示:当其浓度为12.5μg/mL时,对烟草花叶病毒(TMV)在心叶烟枯斑寄主上的侵染抑制率达83.0%;y3对兔血凝集活性滴度为2.5,对人血凝集活性滴度为26,其浓度分别为1.562μg/mL和0.781μg/mL;利用胃癌细胞株MGC803检测y3体外抗肿瘤活性,其IC50为12μg/mL。y3 N端序列为NRDVAACARFIDDFCDTLTP,为一新的蛋白序列。在SWISSPORT上登录号为P83477。
Analysis of genetic diversity among germplasm resource of 57 strains of Coprinus comatus
57株毛头鬼伞遗传多样性分析

JIANG Yu-Ji,XIE Bao-Gui,DENG You-Jin,LIU Xin-Rui,JIANG Hong,
江玉姬
,谢宝贵,邓优锦,刘新锐,江洪

菌物学报 , 2013,
Abstract: 运用SRAP、RAPD、ISSR 3种分子标记技术对来源不同地区的57株毛头鬼伞Coprinus comatus进行了遗传多样性分析,通过3种分子标记进行聚类分析,当相异系数D为0.48时,可以把57株毛头鬼伞分为4类:Ⅰ类包括Co0001;Ⅱ类包括Co0003;Ⅲ类包括Co0005;Ⅳ类包括其余54个菌种。供试的57个菌株间的相异系数范围从0–0.72,具有一定的遗传多态性。但其中有许多菌株两者之间的相异系数为0,说明毛头鬼伞菌种存在着比较严重的同种异名现象。
Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus  [PDF]
Chunjuan Gu, Fei Zheng, Liangkun Long, Jing Wang, Shaojun Ding
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0093912
Abstract: The detail understanding of physiological/biochemical characteristics of individual laccase isoenzymes in fungi is necessary for fundamental and application purposes, but our knowledge is still limited for most of fungi due to difficult to express laccases heterologously. In this study, two novel laccase genes, named lac3 and lac4, encoding proteins of 547 and 532-amino acids preceded by 28 and 16-residue signal peptides, respectively, were cloned from the edible basidiomycete Coprinus comatus. They showed 70% identity but much lower homology with other fungal laccases at protein level (less than 58%). Two novel laccase isoenzymes were successfully expressed in Pichia pastoris by fusing an additional 10 amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser?)tag at N-terminus, and the volumetric activities could be dramatically enhanced from undetectable level to 689 and 1465 IU/l for Lac3 and Lac4, respectively. Both laccases possessed the lowest Km and highest kcat/Km value towards syringaldazine, followed by ABTS, guaiacol and 2,6-dimethylphenol similar as the low redox potential laccases from other microorganisms. Lac3 and Lac4 showed resistant to SDS, and retained 31.86% and 43.08% activity in the presence of 100 mM SDS, respectively. Lac3 exhibited higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes, which may attribute to the relatively higher catalytic efficiency of Lac3 than Lac4 (in terms of kcat/Km) towards syringaldazine and ABTS. The mild synergistic decolorization by two laccases was observed for triphenylmethane dyes but not for anthraquinone and azo dyes.
Chemical Compositions and Antioxidant Activities of 16 Wild Edible Mushroom Species Grown in Anatolia  [PDF]
Ilgaz Akata,Bulent Ergonul,Fatih Kalyoncu
International Journal of Pharmacology , 2012,
Abstract: In this study, chemical compositions and antioxidant activities of 16 wild edible mushrooms (Agrocybe cylindracea, Amanita ceciliae, Armillaria mellea, Boletus reticulatus, Cantharellus cibarius, Chlorophyllum rhacodes, Coprinus comatus, Flammulina velutipes var. velutipes, Lactarius deliciosus, Lactarius salmonicolor, Pleurotus ostreatus, Polyporus squamosus, Rhizopogon roseolus, Russula anthracina, Suillus collinitus and Tricholoma myomyces) were investigated. Antioxidant properties of methanol extracts were studied by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. Among the mushroom extract Amanita ceciliae and Pleurotus ostreatus (96.16 %) showed the most potent radical scavenging activities at 4.51 and 2.72 mg mL-1, respectively. The lowest scavenging activity was exhibited by C. rhacodes (70.46%) at 2.35 mg mL-1.
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