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Brevibacillus laterosporus, a Pathogen of Invertebrates and a Broad-Spectrum Antimicrobial Species  [PDF]
Luca Ruiu
Insects , 2013, DOI: 10.3390/insects4030476
Abstract: Brevibacillus laterosporus, a bacterium characterized by the production of a unique canoe-shaped lamellar body attached to one side of the spore, is a natural inhabitant of water, soil and insects. Its biopesticidal potential has been reported against insects in different orders including Coleoptera, Lepidoptera, Diptera and against nematodes and mollusks. In addition to its pathogenicity against invertebrates, different B. laterosporus strains show a broad-spectrum antimicrobial activity including activity against phytopathogenic bacteria and fungi. A wide variety of molecules, including proteins and antibiotics, have been associated with the observed pathogenicity and mode of action. Before being considered as a biological control agent against plant pathogens, the antifungal and antibacterial properties of certain B. laterosporus strains have found medical interest, associated with the production of antibiotics with therapeutic effects. The recent whole genome sequencing of this species revealed its potential to produce polyketides, nonribosomal peptides, and toxins. Another field of growing interest is the use of this bacterium for bioremediation of contaminated sites by exploiting its biodegradation properties. The aim of the present review is to gather and discuss all recent findings on this emerging entomopathogen, giving a wider picture of its complex and broad-spectrum biocontrol activity.
Anti-Methicillin Resistant Staphylococcus aureus Activity of Brevibacillus laterosporus Strain SA14  [cached]
Walailak Journal of Science and Technology , 2008, DOI: 10.2004/vol5iss1pp47-56
Abstract: We isolated strain SA14 that produced an antibacterial agent against Staphylococcus aureus and clinical isolates of methicillin-resistant S. aureus from air samples and identified it to be Brevibacillus laterosporus using API 50 CHB strips. It showed a broad range of antibacterial activity against bacteria in contaminated drinking water such as Escherichia coli and Pseudomonas and even the opportunistic microorganism Candida albicans, when investigated by the cross streak method. It excreted antimicrobial peptides into culture broth on the first day of cultivation. The peptide molecular weight determined by SDS-PAGE was 116 kDa. Characteristic measurements indicate that the peptides had a relatively gram-positive bacteria inhibitory spectrum, especially, S. aureus and MRSA, when investigated by agar well diffusion. The anti-MRSA activity was not affected by a wide pH, chemical compounds and temperature range.
海带内生真菌Galactomyces geotrichum次生代谢产物改善小鼠的学习记忆能力  [PDF]
- , 2018, DOI: 10.13982/j.mfst.1673-9078.2018.7.001
Abstract: 报道了海带内生真菌白地霉Galactomyces geotrichum次生代谢产物的乙酸乙酯萃取物具有改善小鼠学习、记忆能力的作用。以D-半乳糖所致的阿尔兹海默病(AD)小鼠为模型,采用Morris水迷宫实验测定了不同剂量组的上述萃取物对小鼠血清、肝脏和脑部组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性和丙二醛(MDA)的含量及脑组织中乙酰胆碱转移酶(ChAT)和乙酰胆碱酯酶(AchE)的活性的影响。Morris水迷宫结果显示,试验各剂量组的逃避潜伏期的时间均显著缩短(p<0.05),而空间探索能力也有所改善;与模型组相比,低、中、高剂量组血清、肝脏与脑部组织的SOD、GSH-Px、CAT活力升高,而MDA含量降低,脑部组织中AchE活力下降、ChAT活力上升。上述发现说明,该次代谢产物的乙酸乙酯萃取物对AD模型小鼠具有明显改善学习记忆能力的作用。
This work reported that the ethyl acetate extract of secondary metabolites of endophytic fungus Galactomyces geotrichum could improve the memory and learning ability of mice. With D-galactose-induced the Alzheimer's disease (AD) mice as the model, the influence of different levels of dosages of the extract on the contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in serum, liver and brain of the mice was investigated, using the Morris water maze test. The activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) in mice brain were also evaluated. The results showed that the escape latent period of the mice in each dose group was significantly shortened(p<0.05), and there was also noticable improvement in the spatial probe capability of the mice. Compared with the model group, the activities of SOD, GSH-Px and CAT in serum, liver and brain tissue of the low, middle and high dose groups were increased, while the MDA content decreased, the activity of AchE decreased, as well as that of ChAT increased in brain tissue. The findings indicated that the ethyl acetate extract of secondary metabolites could improve the memory and learning ability of the AD mice.
Optimization of an Antimicrobial Peptide R-1 Production by Brevibacillus laterosporus Lh-1
海洋侧孢短芽孢杆菌Brevibacillus laterosporus Lh-1 产抗菌肽R-1的培养条件优化

郑 媛
,王跃军,孙 谧

微生物学通报 , 2009,
Abstract: To improve and optimize marine antimicrobial peptide R-1 production by a newly isolated Brevibacillus laterosporus Lh-1, Plackett-Burman (PB) design and response surface methodology (RSM) using central composite design was adopted in culture conditions. MINITAB 15.0 was used for planning the experiments, data analysis, contour diagrams and response optimizations. In this study, PB design was undertaken to evaluate the effects of the fifteen factors. By the statistical regression analysis, the significant factors affecting the novel antimicrobial peptide R-1 in submerged fermentation by Br. laterosporus Lh-1 were determined as follows: glucose, peptone and CaCl2. Then a RSM was used to optimize the above critical internal factors, and the optical concentration of the variables were determined as: 15.72 g/L glucose, 6.01 g/L peptone and 3.29 g/L CaCl2. The content of R-1 was increased from 82.15 kU/mL to 116.27 kU/mL.
Genomic and Metabolomic Insights into the Natural Product Biosynthetic Diversity of a Feral-Hog-Associated Brevibacillus laterosporus Strain  [PDF]
Christine M. Theodore, Blake W. Stamps, Jarrod B. King, Lauren S. L. Price, Douglas R. Powell, Bradley S. Stevenson, Robert H. Cichewicz
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090124
Abstract: Bacteria associated with mammals are a rich source of microbial biodiversity; however, little is known concerning the abilities of these microbes to generate secondary metabolites. This report focuses on a bacterium isolated from the ear of a feral hog from southwestern Oklahoma, USA. The bacterium was identified as a new strain (PE36) of Brevibacillus latersporus, which was shown via genomic analysis to contain a large number of gene clusters presumably involved in secondary metabolite biosynthesis. A scale-up culture of B. latersporus PE36 yielded three bioactive compounds that inhibited the growth of methicillin-resistant Staphylococcus aureus (basiliskamides A and B and 12-methyltetradecanoic acid). Further studies of the isolate's secondary metabolome provided both new (auripyrazine) and previously-described pyrazine-containing compounds. In addition, a new peptidic natural product (auriporcine) was purified that was determined to be composed of a polyketide unit, two L-proline residues, two D-leucine residues, one L-leucine residue, and a reduced L-phenylalanine (L-phenylalanol). An examination of the genome revealed two gene clusters that are likely responsible for generating the basiliskamides and auriporcine. These combined genomic and chemical studies confirm that new and unusual secondary metabolites can be obtained from the bacterial associates of wild mammals.
Purification and properties of an antimicrobial substance from marine Brevibacillus laterosporus Lh-1

REN Zhao-zhen,ZHENG Yuan,SUN Mi,LIU Jun-zhong,WANG Yue-jun,

微生物学报 , 2007,
Abstract: An antimicrobial substance produced by Brevibacillus laterosporus isolated from the sea sediment was purified and characterized. The antimicrobial substance was purified by ultrafiltration,DEAE-Sepharose Fast flow chromatography,CM-Sepharose Fast flow chromatography and HPLC reversed phase column chromatography,and after the final purification step,one active fraction was obtained,designated R-1. The molecular weight (MW) was accurately determined by MALDI-TOF-MS as 1608.023 Da. And its pI was determined with Rotofor Cell BIO-RAD to be 8.55. Amino acid analysis of the purified R-1 showed that it was composed of Leu,Tyr,Val,Ile,Lys,Gly,Met,Ser and Ala. Most of them were hydrophobic and neutral amino acid except Lys which was a basic amino acid. And this accorded with pI of R-1. R-1 remained active over a wide temperature range and it also was active over a broad pH rang. R-1 was insusceptible to pancreatin,pepsin and alkaline proteinase. Agar radial diffusion assay showed that R-1 had low minimun bactericidal concentration against Gram-Positive Bacteria such as Streptococcus mutans,Staphylococcus aureaus,Clostridium and Gram-Negative Bacteria such as Escherichia coli,Pseudomonas putrefaciens. And R-1 had antibacterial activities against Candida albicans.
The phylogenetic analysis of 15 Geotrichum strains based on 26S rRNA gene D1/D2 region sequencing
基于26S rDNAD1/D2区序列分析的15株白地霉分子分类学研究

MA Kai,LIU Guang-quan,LI Jin-xi,YAO Su,CHENG Chi,

微生物学报 , 2007,
Abstract: The 26S rRNA gene D1/D2 domain sequences of 15 strains originally identified as Galactomyces geotrichum from the Chinese Industry Culture Collection (CICC) were determined. The results indicated that these strains differed from the type strain of Galactomyces geotrichum and other species of the genus remarkably. Two groups were recognized from the 15 strains which possibly represent 2 novel species of Galactomyces. Further molecular study is needed to confirm their taxonomic status.
Journal of the Chilean Chemical Society , 2008, DOI: 10.4067/S0717-97072008000100011
Abstract: ergosterol 1, peroxyergosterol 2, ergosta-4,6,8(14), 22-tetraen-3-one 3 and 24-ethyl-cholesta-4-ene-3-one 4 were isolated from the cultures of a fungus geotrichum sp. obtained from a marine sediment. it was established that no other sterols were present in the extract. their structures were elucidated by spectroscopic methods
Journal of the Chilean Chemical Society , 2008,
Abstract: Ergosterol 1, peroxyergosterol 2, ergosta-4,6,8(14), 22-tetraen-3-one 3 and 24-ethyl-cholesta-4-ene-3-one 4 were isolated from the cultures of a fungus Geotrichum sp. obtained from a marine sediment. It was established that no other sterols were present in the extract. Their structures were elucidated by spectroscopic methods
Dyeing of hydrophobic fabrics with disperse dyes
Journal of the Serbian Chemical Society , 2001,
Abstract: A series of disperse dyes has been synthesized by diazotisation of 2,6-dibromo-4-nitroaniline and coupled with various N-arylmaleimides. The dyes were characterized by IR spectral studies, visible absorption spectroscopy and elemental analysis. All the dyes were applied as disperse dyes on nylon, cellulose triacetate and polyester fabrics. These dyeswere found to give yellowish orange to deep brown shades with very good depth, levelness and brightness on different fabrics. The percentage dye bath exhaustion and fixation on fabrics were found to be very good. The light, washing, rubbing, perspiration and sublimation fastness properties of the dyed fabrics were found to be good to excellent.
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