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Transient and Stable GFP Expression in Germ Cells by the vasa Regulatory Sequences from the Red Seabream (Pagrus major)
Fan Lin, Qinghua Liu, Mingyou Li, Zhendong Li, Ni Hong, Jun Li, Yunhan Hong
International Journal of Biological Sciences , 2012,
Abstract: Primordial germ cells (PGCs) are the precursors of gametes responsible for genetic transmission to the next generation. They provide an ideal system for cryopreservation and restoration of biodiversity. Recently, considerable attention has been raised to visualize, isolate and transplant PGCs within and between species. In fish, stable PGC visualization in live embryo and individual has been limited to laboratory fish models such as medaka and zebrafish. One exception is the rainbow trout, which represents the only species with aquaculture importance and has GFP-labeled germ cells throughout development. PGCs can be transiently labeled by embryonic injection of mRNA containing green fluorescence protein gene (GFP) and 3'-untranslated region (3'-UTR) of a maternal germ gene such as vasa, nos1, etc. Stable PGC labeling can be achieved through production of transgenic animals by some transcriptional regulatory sequences from germ genes, such as the vasa promoter and 3'-UTR. In this study, we reported the functional analyses of the red seabream vasa (Pmvas) regulatory sequences, using medaka as a model system. It was showed that injection of GFP-Pmvas3'UTR mRNA was able to label medaka PGCs during embryogenesis. Besides, we have constructed pPmvasGFP transgenic vector, and established a stable transgenic medaka line exhibiting GFP expression in germ cells including PGCs, mitotic and meiotic germ cells of both sexes, under control of the Pmvas transcriptional regulatory sequences. It is concluded that the Pmvas regulatory sequences examined in this study are sufficient for germ cell expression and labeling.
The histopathology of Scomber japonicus infection by Nematobothriun scombri (Trematoda: Didymozoidae) and of larval anisakid nematode infections in the liver of Pagrus pagrus
Eiras, J. C.;Rego, A. A.;
Memórias do Instituto Oswaldo Cruz , 1987, DOI: 10.1590/S0074-02761987000200001
Abstract: the histopathology induced by nematobothrium scombri (trematoda) in scomber japonicus and of larval anisakid nematodes in pagrus pagrus is described. nematodes larvae occurred within the liver capsule and n. scombri occurred within nodules in the opercula. the fishes were collected off the coast of rio de janeiro state.
Larvas de Anisakidae na musculatura do pargo, Pagrus pagrus, no Estado do Rio de Janeiro, Brasil
Saad, Caroline D. R.;Luque, José L.;
Revista Brasileira de Parasitologia Veterinária , 2009, DOI: 10.4322/rbpv.018e1014
Abstract: musculature of 36 specimens of pagrus pagrus from the coastal zone of the state of rio de janeiro were examined to study larval of anisakid nematodes between january and may 2008. a total of 24 larval of anisakis sp., contracaecum sp., hysterothylacium sp. and raphidascaris sp. were collected infecting seven fishes (19.4%). larval hysterothylacium sp. and contracaecum sp. showed highest prevalence and hysterothylacium sp. highest parasite abundance. this is the first record of larval anisakids in the somatic musculature of p. pagrus.
vasa基因研究进展  [PDF]
陈玉冬,邹志华,王艺磊,张子平
动物学杂志 , 2010,
Abstract: DEAD-box家族基因编码一类ATP依赖的RNA解旋酶。经系统进化分析可将该家族蛋白分为VASA、PL10和p68三个亚家族。其中,vasa基因最先在果蝇(Drosophilamelanogaster)中被发现,在许多动物中都已经克隆得到其同源基因,研究显示,vasa基因在生殖细胞系中特异性表达,在许多生物中为生殖细胞形成和配子发生所需。有趣的是在果蝇中VASA蛋白是生殖质的组成部分,而在斑马鱼(Daniorerio)中vasamRNA才是生殖质的组成部分。本文主要综述了vasa基因及其蛋白的结构、功能、表达和作为原生殖细胞分子标记物的应用等方面的内容,并展望了其研究前景。
Early life stages of development of the red porgy Pagrus pagrus (Pisces, Sparidae) in captivity, Argentina
Machinandiarena,Laura; Müller,Mónica; López,Andrea;
Investigaciones marinas , 2003, DOI: 10.4067/S0717-71782003000100002
Abstract: the early life history, from egg to juvenile, of red porgy pagrus pagrus, in captivity is described. yolk larvae were hatched 50 hours after fertilization, at 18°c water temperature. the opening of the mouth took place between the third and fourth day after hatching, corresponding to 2.6-2.8 mm notochordal length. the transformation stage began at 9 mm standard length, approximately 40 days after hatching. the appearance of the fins favored the displacement of pelagic larvae towards the bottom, acquiring their demersal habit during the juvenile stage
Early life stages of development of the red porgy Pagrus pagrus (Pisces, Sparidae) in captivity, Argentina Desarrollo de los estadios iniciales del besugo Pagrus pagrus (Pisces, Sparidae) en cautiverio, Argentina  [cached]
Laura Machinandiarena,Mónica Müller,Andrea López
Investigaciones Marinas , 2003,
Abstract: The early life history, from egg to juvenile, of red porgy Pagrus pagrus, in captivity is described. Yolk larvae were hatched 50 hours after fertilization, at 18°C water temperature. The opening of the mouth took place between the third and fourth day after hatching, corresponding to 2.6-2.8 mm notochordal length. The transformation stage began at 9 mm standard length, approximately 40 days after hatching. The appearance of the fins favored the displacement of pelagic larvae towards the bottom, acquiring their demersal habit during the juvenile stage Se describen los estadios iniciales, desde huevo hasta juvenil, del besugo Pagrus pagrus obtenidos en cautiverio. Las larvas vitelinas eclosionan 50 horas posteriores a la fertilización, a una temperatura de 18°C. La apertura de la boca se produce entre el tercer y cuarto día posteriores a la eclosión, correspondiendo a 2,6-2,8 mm de longitud de la notocorda. La etapa de transformación comienza en larvas de 9 mm de longitud estándar y 40 días, aproximadamente. La formación de las aletas favorece el desplazamiento de las larvas pelágicas hacia el fondo, adquiriendo hábito demersal durante el estadio juvenil
Efficiency of co-feeding red porgy (Pagrus pagrus L.) larvae with live and compound diet
Aristizábal,Eddie O; Suárez,Julieta;
Revista de biología marina y oceanografía , 2006, DOI: 10.4067/S0718-19572006000200008
Abstract: the red porgy (pagrus pagrus) is an important commercial and recreational species with very good prospect for aquaculture. because significant mortalities, the mass production of this species is limited. this paper describes three weaning strategies to investigate their effects on the survival and growth of red porgy larvae reared in laboratory: a) traditional three-step weaning (rotifers - artemia - diet) (control); b) one-step early weaning by co-feeding (rotifers - artemia + diet), and c) one-step direct early weaning (rotifers - diet). sigificative differences were recorded among treatments (p<0.05). best survival rate (45.6 ± 7.6%) and growth (7.4 ± 0.5 mm) were found in treatment b. results indicate that co-feeding red porgy larvae with inert and live diets from day 20 after hatching is a promising weaning strategy in the hatchery of this species
Listonella anguillarum Isolated from Hatchery-Cultured Red Porgy Pagrus pagrus in Turkey
Jale Korun,Mehmet Gokoglu
Journal of Animal and Veterinary Advances , 2012,
Abstract: A Listonella anguillarum infection in cultured red porgy (Pagrus pagrus) is documented for the first time in this report. The infection was identified in a commercial hatchery in the Aegean near Bodrum, Turkey. Diseased red porgy were characterized by lethargy, anorexia, fin erosion and abdominal swelling. Haemorrhagies on the operculum, fins and over the surface of the body were seen. Internally, the liver and the kidney were pale. The spleen was enlarged. Ascites in the abdominal cavity was also observed. The identification of bacterial strains isolated from the affected fish was based on morphological (Gram staining and wet-mouth preparation), physiological and biochemical tests (API 20E). Mono-Va agglutination kit was also included to confirm the identification of the isolated bacteria. The principal histological changes in this study were haemosiderosis in the spleen and kidney, a massive depletion of haemopoietic elements in the spleen, haemorrhagies in the liver, intestinal walls and under the muscle tissue.
A new species of Anoplodiscus (Monogenea: Anoplodiscidae) parasitic on Pagrus pagrus (Osteichthyes: Sparidae) from the coastal zone of the State of Rio de Janeiro, Brazil
Paraguassú, Aline R;Luque, José L;Alves, Dimitri R;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000800022
Abstract: a new species of anoplodiscus (monogenea, anoplodiscidae), parasitic on gills of the red porgy, pagrus pagrus, from the coastal zone of the state of rio de janeiro, brazil, is described and illustrated. the new species can be differentiated from the other species of this genus by the shape of the accessory piece of the copulatory complex, and the length of the vagina. this is the first record of a species of anoplodiscus in the neotropical region.
A new species of Anoplodiscus (Monogenea: Anoplodiscidae) parasitic on Pagrus pagrus (Osteichthyes: Sparidae) from the coastal zone of the State of Rio de Janeiro, Brazil  [cached]
Paraguassú Aline R,Luque José L,Alves Dimitri R
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: A new species of Anoplodiscus (Monogenea, Anoplodiscidae), parasitic on gills of the red porgy, Pagrus pagrus, from the coastal zone of the State of Rio de Janeiro, Brazil, is described and illustrated. The new species can be differentiated from the other species of this genus by the shape of the accessory piece of the copulatory complex, and the length of the vagina. This is the first record of a species of Anoplodiscus in the Neotropical region.
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