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Antioxidant Activity and Hepatoprotective Potential of Black Seed, Honey and Silymarin on Experimental Liver Injuries Induced by CCl4 in Rats
Mona E. Khadr,Karam A. Mahdy,Karima A. El-Shamy,Fatma A. Morsy
Journal of Applied Sciences , 2007,
Abstract: The possible antioxidant activity and hepatoprotective potential of black seed honey and silymarin on CCl4 induced liver injuries in rats was investigated. Fifty male rats were used in this study and divided into five groups, 10 rats each. Group 1 served as a control; group 2 injected 1 mL kg-1 day-1 CCl4 intraperitoneally 3 times a week for 4 week, groups 3, 4 and 5 subjected to the same injection of CCl4 and co-treatment with black seed, honey and silymarin (50 mg kg-1 b.wt.), respectively, daily by stomach tube for 4 weeks. Blood and tissue samples were taken for biochemical and histopathological studies. The results revealed that CCl4 administration caused significant elevations in the levels of MDA, NO, MMP-2, AST and ALT. Histopathological observations showed severe damage in the liver. Its fibrotic areas were measured using Image Analyzer. Combined treatment with CCl4 and black seed, honey and silymarin showed marked improvement in antioxidant status and in histopathological findings as well as reductions in the fibrotic areas. These results concluded that black seed, honey and silymarin have protective characteristics against CCl4-induced rat liver injury through potentiation of antioxidant capacity of liver cells and prevention of oxidative stress that accompanied with CCl4 hepatotoxicity. The protective effect was higher in silymarin followed by black seed then honey.
V. R. Mohan et al.
International Journal of Pharmaceutical Sciences and Research , 2012,
Abstract: The present study was designed to screen and evaluate the hepatoprotective and antioxidant activity of ethanol extracts of leaf and bark of Naringi crenulata (Roxb) Nicolson (NCL, NCB) against CCl4 induced hepatotoxicity in rats. Liver functions were assessed by the activities of liver marker enzymes, SGOT, SGPT, ALP, total protein, albumin, globulin, total, conjugated and unconjugated bilirubins. It also exhibited antioxidant activity by showing the increased activity of SOD, CAT, GPx and GRD and decreased in TBARS compared to CCl4 treated groups. Silymarin, a known hepatoprotective drug is used for comparison. The plant extracts were effective in protecting liver against injury induced by CCl4 in rats.
Thangakrishnakumari S,Nishanthini A,Muthukumarasamy S,Mohan V.R.
International Journal of Research in Ayurveda and Pharmacy , 2012,
Abstract: The aim of the study was to investigate the hepatoprotective activity of ethanol extract of whole plant of Canscora perfoliata in CCl4 induced hepatotoxic rats. Administration of hepatotoxins (CCl4) showed significant elevation of serum SGOT, SGPT, ALP, bilirubin, conjugated, unconjugated bilirubin and lipid peroxidation. Treatment with Canscora perfoliata (150 and 300mg/kg) significantly reduced the above mentioned parameters. The plant extract also enhanced the antioxidant activity. The ethanol extract of Canscora perfoliata have significant effect on the CCl4 induced hepatotoxicity animal models.
Hepatoprotective Action of Radix Paeoniae Rubra Aqueous Extract against CCl4-Induced Hepatic Damage  [PDF]
Ruidong Li,Wenyuan Guo,Zhiren Fu,Guoshan Ding,You Zou,Zhengxin Wang
Molecules , 2011, DOI: 10.3390/molecules16108684
Abstract: In the present study the capacity of Radix Paeoniae Rubra aqueous extract (RPRAE) as an antioxidant to protect against carbon tetrachloride (CCl4)-induced oxidative stress and hepatotoxicity in Wistar rats was investigated. Six groups of rats were used. Radix Paeoniae Rubra aqueous extract (100 or 200 or 300 mg/kg of bw) or bifendate (100 mg/kg of bw) were given daily by gavage to the animals on 28 consecutive days to elucidate the protective effects against CCl4-induced hepatotoxicity. The 20% CCl4/olive oil was gavage of gastric tube twice a week (on the third and seventh days of each week). The animals of normal control group were given only vehicle. The animals of CCl4-treated group were administered with CCl4 twice a week (on the third and seventh days of each week) and with vehicle on rest of the days. The test materials were found effective as hepatoprotective agents, as evidenced by plasma and liver biochemical parameters. Therefore, the results of this study show that Radix Paeoniae Rubra aqueous extract can protect the liver against CCl4-induced oxidative damage in rats, and the hepatoprotective effects might be correlated with its antioxidant and free radical scavenger effects.
Hepatoprotective and antioxidant effect of Carissa spinarum root extract against CCl4 and paracetamol-induced hepatic damage in rats  [cached]
Karunakar Hegde, Arun B. Joshi
Bangladesh Journal of Pharmacology , 2010,
Abstract: Ethanolic extract of the roots of C. spinarum was evaluated for hepato-protective and antioxidant activities in rats. Oral pre-treatment with ethanolic extract (100, 200 and 400 mg/kg) showed significant hepatoprotective activity against CCl4 and paracetamol-induced hepatotoxicity by decreasing the activities of bilirubin and lipid peroxidation, and significant increase in the levels of uric acid, glutathione, super oxide dismutase, catalase and protein in a dose dependent manner, which was confirmed by the decrease in liver wet weight and histopathological examination. The extract possessed strong antioxidant activity. This suggests that the hepatoprotective activity of C. spinarum is possibly attributed to its free radical scavenging properties.
P Royal Frank,V Suresh,G Arunachalam,SK Kanthlal
International Research Journal of Pharmacy , 2012,
Abstract: Oxidative damage is involved in the pathogenesis of various hepatic injuries. In the present study, the protectivity of methanolic extract of leaves of Adiantum incisum forsk (MEAI), as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in Albino Wistar rats was investigated. Intraperitoneal injection of CCl4, produced a marked elevation in the serum levels of serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin, and decrease in the total protein. Histopathological analysis of the liver of CCl4-induced rats revealed marked liver cell necrosis with inflammatory collections that were conformed to increase in the levels of SOD, GPx, LPO and CAT. Daily oral administration of methanolic extract of A.incisum forsk at 100 and 200 mg/kg doses for 10 days produced a dose-dependent reduction in the serum levels of liver enzymes. Treatment with A.incisum forsk normalized various biochemical parameters of oxidative stress and was compared with standard Silymarin. Therefore, the results of this study show that A.incisum forsk can be proposed to protect the liver against CCl4-induced liver damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenger effects.
Hepatoprotective Potential of Extracts from Seeds of Areca catechu and Nutgalls of Quercus infectoria  [PDF]
Pimolpan Pithayanukul,Saruth Nithitanakool,Rapepol Bavovada
Molecules , 2009, DOI: 10.3390/molecules14124987
Abstract: Aqueous extracts from seeds of Areca catechu L. (Arecaceae) (AC) and nutgalls of Quercus infectoria Oliv. (Fagaceae) (QI) were investigated for their hepatoprotective potential by studying their antioxidant capacity using four different methods, by determining their in vitro anti?inflammatory activity against 5-lipoxygenase, and by evaluating their hepatoprotective potential against liver injury induced by carbon tetrachloride (CCl4) in rats. AC and QI extracts exhibited potent antioxidant and anti-inflammatory activities. Treatment of rats with AC and QI extracts reversed oxidative damage in hepatic tissues induced by CCl4. It is suggested that extracts rich in either condensed or hydrolysable tannins and known for their potent antioxidant and anti-inflammatory activities, may potentially confer protection against oxidative stress?induced liver injury. These data should contribute to evidence-based traditional medicines for anti-inflammatory and hepatoprotective effects of both extracts.
Potent hepatoprotective effect in CCl4-induced hepatic injury in mice of phloroacetophenone from Myrcia multiflora
EA Ferreira, EF Gris, KB Felipe, JF Gomes Correia, E Cargnin-Ferreira, DW Filho, RC Pedrosa
Libyan Journal of Medicine , 2010,
Abstract: Background: This study investigated the hepatoprotective effect and antioxidant properties of phloroacetophenone (2',4',6'-trihydroxyacetophenone - THA), an acetophenone derived from the plant Myrcia multiflora. Material & Method: The free radical scavenging activity in vitro and induction of oxidative hepatic damage by carbon tetrachloride (CCl4) (0.5 ml/kg, i.p.) were tested in male Swiss mice (2595 g). Results: This compound exhibited in vitro antioxidant effects on FeCl2-ascorbate-induced lipid peroxidation (LPO) in mouse liver homogenate, scavenging hydroxyl and superoxide radicals, and 2,2-diphenyl-1-picrylhydrazyl. The in vivo assays showed that THA significantly (p<0.01) prevented the increases of hepatic LPO as measured by the levels of thiobarbituric acid-reactive substances, mitochondrial swelling. It also protected hepatocytes against protein carbonylation and oxidative DNA damage. Consistent with these observations, THA pre-treatment normalized the activities of antioxidant enzymes, such as catalase, glutathione peroxidase, and superoxide dismutase, and increased the levels of reduced glutathione (GSH) in CCl4-treated mice. In addition, THA treatment significantly prevented the elevation of serum enzymatic activities of alanine amino transferase, aspartate amino transferase, and lactate dehydrogenase, as well as histological alterations induced by CCl4. Silymarin (SIL) (24 mg/kg), a known hepatoprotective drug used for comparison, led to a significant decrease (p<0.01) in activities of theses enzymes in way very similar to that observed in pre-treatment with THA. Conclusion: These results suggest that the protective effects are due to reduction of oxidative damage induced by CCl4 resulting from the antioxidant properties of THA.
Hepatoprotective and Antioxidant effect of Polygala rosmarinifolia Wight & Arn against CCl4 induced hepatotoxicity in rats.  [PDF]
M. Alagammal,M. Packia Lincy,V.R. Mohan
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: Carbon tetrachloride (CCl4) intoxicated rats showed significant elevation in serum enzymes, bilirubin and lipid peroxidation of the liver tissues and reduction in serum total protein, superoxide dismutase, catalase, reduced glutathione peroxide activity. Treatment with ethanol extract of Polygala rosmarinifolia whole plant altered the above parameters to the levels of near normal. All the above results were comparable with the standard drug silymarin (100mg/kg) treated group. Thus the present study ascertains that the ethanol extract of Polygala rosmarinifolia whole plant possesses significant hepatoprotective activity.
Patil Prakash,K Prasad,M Nitin,M Vijay Kumar
International Journal of Research in Ayurveda and Pharmacy , 2011,
Abstract: In the present study, Calotropis procera (Asclepediaceae) was evaluated for its possible hepatoprotective and antioxidant potential. Hepatoprotective activity of the methanol extract (MCP) of the root bark was determined using carbon tetrachloride (CCl4) induced liver injury in rats. MCP extract evaluated, at an oral dose of 200 and 400 mg kg1. The animals were weighed each and divided in groups of six. Liver damage was achieved by injecting CCl4 in olive oil (1:1) 0.8 mL kg-1. The treatment groups pretreated with above extracts. Silymarin was used as reference standard drug. At the end of 7 days, blood was collected, liver extracted, weighed, processed for histopathological assessments and for antioxidant activity. The MCP exhibited a significant (p<0.05) hepato-protective effect by lowering the elevated serum levels of serum transaminases (AST and ALT), Alkaline phosphatase (ALP), total and direct serum bilirubin, cholesterol and significantly increasing high density lipoprotein (HDL) and moderately increasing total protein and albumin. These biochemical observations were supplemented by histopathological examination of liver sections. Further, the effects of the MCP extract on antioxidant enzymes also have been investigated to elucidate the possible mechanism of its hepatoprotective activity. The MCP extract exhibited a significant effect(p<0.05) in a dose dependent manner by modifying the levels of reduced glutathione, super oxide dimutase, catalase activity and malondialdehyde equivalent, an index of lipid peroxidation of the liver. These findings suggest that the MCP extract exhibited a dose dependent significant effect on hepatoprotective and antioxidant potential which revitalidates the use of this plant for the treatment of liver toxicity in oriental traditional medicine.
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