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The Protein Profiles of Trichophyton rubrum by SDS-PAGE
M Mahmoudi Rad,F Zaini,M Pezeshki,P Kordbacheh
Iranian Journal of Public Health , 2001,
Abstract: In the present study, we investigated total cell protein patterns of ten isolates of Trichophyton rubrum by SDS-PAGE on 12.5% polyacrylamide resolving gels. Twenty-two protein bands were detected with molecular weights in the range of 23.2 to 131.8 KD. Proteins of 23.2, 25.4, 26.7, 28.2, 30.2, 33.1, 34.7, 38,41.7, 47.9, 52.5, 56.2, 61.7, 67.6, 70.8, 75.9, 80, 84.1, 93.3, 102.3, 114.8, 131.8 were present but their frequencies varied among the isolates. Protein bands of 23.2, 38, 47.9, 52.5, 84.1 were common among the isolates and could be specific to recognize species differences. Protein analysis by SDS-PAGE could be considered a useful technique in identifying differences among the dermatophyte isolates.
SDS-PAGE Analysis of the Eggs of Bombyx mori
JIANG Chun-bao,WANG Lin-ling,WANG Yu,WANG Li-jun
Journal of Chongqing Normal University , 2012,
Abstract: "The paper discusses the effects of different means to extract the protein of eggs of Bombyx mori as well as to find the best conditions of SDS-PAGE to separate the proteins of eggs of B. mori. Trichloroacetic acid (TCA) precipitation, acetone precipitation and trichloroacetic acid-acetone (TCA-acetone) precipitation were used to extract the protein of eggs of B. mori. The protein was separated by different systems of homogeneous gel in a series of concentrations from 6% (separation gel) ~ 12%(spacer gel) to 6%(separation gel) ~ 15%(spacer gel) to search the most appropriate way of extracting the protein of eggs of B. mori and the conditions of electrophoresis. Protein maps of different homogeneous gel concentrations (6% ~ 12%, 6% ~ 15%) and different protein extractionmethods were obtained. Then the efficiency of the different maps was compared. It showed that removing the salt by TCA-acetone method and dissolving the protein by the lysis buffer consists of urea (9 mol·L-1)、CHAPS(4%)and DTT(1%) were the best way to ex-tract the proteins of eggs of B. mori. Meanwhile the suitable gradient of the homogeneous gel is 6% ~ 12%. The optimized method wasexplicated to analysis the differences between the eggs of the normal B. mori and whose infected by Nosema bombycis. Finally, the 66 kD protein band and 85 kD protein bands were gained and speculated to associate with the infection of Nosema bombycis."
黑麦Secalin蛋白的SDS—PAGE检测法  [PDF]
司红起,马传喜
麦类作物学报 , 2003, DOI: 10.7606/j.issn.1009-1041.2003.04.146
Abstract: 对84份中国冬小麦栽培品种分别使用醇提法和水提法提取其胚乳贮藏蛋白。并用SDS-PAGE检测黑麦Secalin蛋白的有无。以此来估算我国舍有1B/1R易位的冬小麦品种的频率,结果表明:醇捉SDS-PAGE黑麦Se-calin蛋白捡出率为46.4%,水提SDS-PAGE黑麦Secalin蛋白检出率为56.0%。后者比前者灵敏;眼提SDS-PAGE和水提SDS-PAGE都是检测黑麦Secalin蛋白的有效方法,若同时使用效果套更好,因为它们可以互相补充,由此检测可知,我国含有1B/1R易位的冬小麦品种的频率高于59.5%。
The Significance of Cuticular Features, Petiole Anatomy and SDS-PAGE in the Taxonomy of the Lauraceae
E. A. Kamel,M.H.A. Loutfy
Pakistan Journal of Biological Sciences , 2001,
Abstract: SDS-PAGE of seed protein, petiole anatomy and leaf cuticular features were used to re-assess the taxonomic relationships between eight taxa presenting four genera of the Lauraceae, viz. Apollonias Nees (one species), Cinnamomum Schaeff (three species), Laurus L. (two species) and Persea Mill. (one species with two varieties). The data obtained were analyzed by Ntsys-pc program package using the UPGMA clustering method. The produced dendrograms were discussed. SDS-PAGE analysis showed a close relationship between Apollonias and Persea. Domatia and certain unique features were recorded in the leaf epidermis of Apollonias. Considerable variations were evident between the two varieties of Persea and a re-evaluation of their status was suggested. The validity of SDS-PAGE of seed protein criteria in the Lauraceae was confirmed
不同地区板蓝根蛋白sds-page分析  [PDF]
?杨欣,王秋红,王悦,郭慧,邢娜,匡海学
中国中医药信息杂志 , 2015, DOI: 10.3969/j.issn.1005-5304.2015.07.024
Abstract: 目的采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(sds-page)技术鉴别不同地区板蓝根,为板蓝根药材的鉴别和质量评价提供依据。方法采用sds-page技术,建立黑龙江和河北地区板蓝根蛋白图谱。结果黑龙江板蓝根蛋白约含13条亚基,主要为10.5kd、23.0kd、24.9kd、44.1kd4种亚基,河北板蓝根蛋白约含12条亚基,主要为55.6kd、45.9kd、34.3kd、18.9kd、12.4kd、10.5kd6种亚基。结论sds-page技术可以用于鉴别不同地区的板蓝根。
The protein profiles of Epidermophyton Floccosum var. pigmented by SDS-PAGE
Pakshir K,Zini F,Aliakhooni E
Tehran University Medical Journal , 1999,
Abstract: In the present study, we investigated total cell protein patterns of ten isolates of Epidermophyton Floccosum Var.pigmented by SDS-PAGE on 10% polyacrylamide resolving gels. Densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 KD proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27, 29, 30, 32.5, 34, 35.5, 37, 39.5, 40.5, 43, 47.5, 50, 63, 68, 75, 79, 82.5, 74.5, 90, 94, 96, 97-5 KD were present but their frequencies varied among the isolates. Protein bands of 18.4, 19.7, 20.1, 27, 29, 34, 37, 43, 47.5, 50, 63, 79, 94, KD were stronger and common among the isolates and could be specific to recognize genus differences. Protein analysis by SDS-PAGE could be considered an useful technique in identifying differences among the dermatophyte isolates.
Genetic Diversity in Brassica Species Using SDS-PAGE Analysis
Rahman Md. Mukhlesur,Yutaka Hirata
Journal of Biological Sciences , 2004,
Abstract: Eighty five different cultivars of Brassica rapa, B.juncea, B.napus, B. carinata, B. oleracea and hexaploid Brassica, collected from Bangladesh, Japan, China and Denmark, were analyzed for seed and leaf protein variations by SDS-PAGE to identify the polymorphic genetic markers for evaluation of genetic resources. Ten polymorphic markers were identified from seed protein and no identifiable polymorphic band was found from leaf protein. However, polymorphic markers clearly distinguished these Brassica species. Brassica rapa var. `yellow sarson` of Bangladesh origin showed uniquely identifiable four polymorphic bands for seed protein in contrast to the other B.rapa of brown-seeded type. The Bangladeshi and Japanese cultivars of B. rapa differed among protein quantity. Analytical results of SDS-PAGE for seed protein showed that hexaploid Brassica has the highest indices, such as % of polymorphic band, the degree of phenotypic diversity (Ho), diversity value for genetic marker (HEP) and the sum of the effective number of alleles (SENA). The genetic diversity values of hexaploid Brassica were followed by amphidiploid (B. napus, B. juncea, B. carinata) and diploid (B. oleracea, B. rapa) species, respectively.
小麦地方品种SDS-PAGE分析  [PDF]
王子宁,郭北海
华北农学报 , 1992, DOI: 10.3321/j.issn:1000-7091.1992.02.006
Abstract: 用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS—PAGE),分析了河北省611份小麦地方品种的麦谷蛋白高分子量(HMW)亚基构成,并依据SDS—PAGE图谱对地方品种的同名异种作了鉴别分析。结果表明,河北省地方品种的麦谷蛋白HMW亚基分布是很单一的,且类型较少,以Glu-Alc(频率97.1%)、Glu-Blb(80.7%)和Glu-D1a(90.5%)为其优势等位基因,表明地方品种烘烤品质一般较差,同时又具有很特殊的亚基类型:有两个频率较高的异常类型,其等位基因可能是最近公布的Glu-Blaj(只编码8亚基)和Glu-Dlk(只编码2亚基),说明我国古老地方品种是一特殊小麦群体类型。同名品种中具有相同SDS—PAGE图谱的品种认为是同一基因型,具有很高的遗传相似性。虽然其中有少数其它基因型,我们认为它们是种植过程中自然变异的结果。
Coupling native page/activity-staining with SDS-PAGE/immunodetection for the analysis of glutamine synthetase isoforms in spinach
Dragi?evi? M.,Tanackovi? Vanja,Mi?i? Danijela,Cveti? Tijana
Archives of Biological Sciences , 2011, DOI: 10.2298/abs1104965d
Abstract: Glutamine synthetase (GS) is a key nitrogen-assimilating enzyme in plants and a target for the broad-spectrum herbicide glufosinate. Understanding its kinetic and structural properties is of major agricultural importance. Spinach (Spinacia oleracea) is classified as a plant expressing only chloroplastic GS activity. We have analyzed soluble proteins in the spinach by coupling native polyacrylamide gel electrophoresis (PAGE)-activity detection, based on phosphate precipitation, with SDS-PAGE/immunoblotting. One cytosolic (GS1) isoform from the roots and two chloroplastic (GS2) isoforms expressed in leaves were resolved by native PAGE. The identity of the obtained bands was established by the application of GS-specific inhibitors, L-methionine sulfoximine and glufosinate. Examination by sodium dodecyl sulfate (SDS)-PAGE/ Western analysis with anti-GS antibodies, confirmed the identity of the active bands and revealed that both chloroplastic isoforms are composed of 44 kDa subunits, while the cytosolic isoform consists of 40 kDa subunits. The presence of more GS2 isozymes than encoded in the spinach genome is discussed in terms of posttranslational modifications.
Evaluation of Different Wheat Varieties by SDS-PAGE Electrophoresis
Mohd Shuaib,Ikhtiar Khan,Zahir Ali,Waqar Ali
Pakistan Journal of Biological Sciences , 2007,
Abstract: Wheat seed-storage proteins represent not only an important source of food and energy but it is also involved in the determination of bread-making quality. Wheat grains of thirteen wheat varieties were collected from different ecological regions of Pakistan. The variability of seed storage-proteins was analyzed by using SDS-PAGE electrophoresis. Electrophorogram for each variety was scored and presence or absence of each band noted and entered in a binary data matrix. Based on electrophoresis band spectra, Jaccard‘s Similarity Index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in developing countries like Pakistan.
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