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Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
中国科学院研究生院学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。 在把作丹皮用的栽培牡丹(凤丹)描述为新种P. ostii后,观赏的栽培牡丹仍然被统称为P. suffruticosa。 但在P. suffruticosa复合体内, P. rockii, P. jishanensis, P. qiui 和P. suffruticosa ssp. yinpingmudan 等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P. suffruticosa “是牡丹组数
Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
植物分类学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。在把作丹皮用的栽培牡丹(凤丹)描述为新种P.ostii后,观赏的栽培牡丹仍然被统称为P.suffruticosa。但在P.suffruticosa复合体内,P.rockii,P.jishanensis,P.qiui和P.suffruticosa ssp.yinpingmudan等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P.suffruticosa“是牡丹组数种植物复合体”的说法。本文在详细论述P.suffruticosa复合体分类历史的基础上,根据我们自己对野生类型和栽培类型的考察研究,认为观赏栽培牡丹的形成是多元的,并且包含几个物种;P.suffruticosa有明确的概念,它是观赏栽培牡丹的组成部分,而不代表栽培牡丹的全部,更不是数种植物的复合体。我们认为应根据形态特征正确鉴定观赏栽培牡丹,不应把它们一概鉴定为P.suffruticosa。通过文献和标本引证,文中界定了P.suffruticosa的范围。此外,把P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen作为P.suffruticosa ssp.yinpingmudan D.Y.Hong,K.Y.Pan&Z.W.Xie的异名,并且认定P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen ssp.henanensis(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen是不合法名称。
Sequential Analysis of Double Indexes of Common and Variant Peak Ratios in Ultraviolet Fingerprint of Paeonia Suffruticosa Andr. Root Bark
丹皮紫外指纹图谱共有峰率和变异峰率双指标序列分析

Yuan Jiurong,Yue Chunhu,Zou Huabin,Wang Aiwu,
袁久荣
,岳春华,邹华彬,王爱武

世界科学技术-中医药现代化 , 2004,
Abstract: Objective: To analyze the ultraviolet fingerprint of the root bark of Paeonia Suffruticosa Andr. and other medicinal crops of its kind by the method of sequential analysis of the double indexes of common and variant peak ratios in ultraviolet fingerprint. Method: In this article three solvents-Chloroform, absolute ethel alcohol and water are introduced to systematically extract the components of the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in different polar zones, examine their ultraviolet fingerprints and study the differences of the samples. Result: This method is able to accurately identify the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in detail. Conclusion: Tow or several samples can be reliably identified by the method of sequential analysis of the double indexes of common and variaut peak ratios.
Dynamic changes in enzyme activities and phenolic content during in vitro rooting of tree peony (Paeonia suffruticosa Andr.) plantlets  [PDF]
Songlin He
Maejo International Journal of Science and Technology , 2011,
Abstract: The dynamic changes of phenolic content and peroxidase (POD), polyphenol oxidase (PPO), indole-3-acetic acid oxidase (IAAO) and phenylalanine ammonia lyase (PAL) activities were assessed during the in vitro rooting process of three cultivars of tree peony (Paeonia suffruticosa Andr.). These changes in enzyme-related activity and phenolic content__observed at the level of the whole plant__differed during the first 20 days of the rooting process in easy-to-root ‘Feng Dan Bai’ cultivar and difficult-to-root ‘Wu Long Peng Sheng’ and ‘Tai Ping Hong’ cultivars, and in most cases they were actually opposite. The ease with which ‘Feng Dan Bai’ was able to root was closely related to the activity of all four enzymes (POD, PPO, IAAO, PAL) as well as to the phenolic content.
PRELIMINARY REPORT OF RAPD ANALYSIS IN PAEONIA SUFFRUTICOSA SUBSP.SPONTANEA AND PAEONIA ROCKII
矮牡丹与紫斑牡丹RAPD分析初报

Pel Yan-long Zou Yu-ping Yin Zhen Wang Xiao-quan Zhang Zhi-xian Hong De-yuan,Laboratory of Systematic,Evolutionary Botany,
裴颜龙
,邹喻苹

植物分类学报 , 1995,
Abstract: Ten different oligonucleotide primers of arbitrary sequence were used in Capillary and Air Thermocycler to amplify genomic total DNA of Paeonia suffruticosa subsp. spon-tanea and P. rockii isolated from several local populations in Shanxi, Shaanxi and Gansu provinces. Under the strictly standardized amplification condition for all the primers, these primers yielded clear and reproducible bands corresponding to amplified products and separable by agarose gel electrophoresis. Among a total of 71 bands amplified, 16(22. 5%) were polymorphic in a single individual of P. suffruticosa subsp. spontanea, while among a total of 76 bands 21(27. 6%)were polymophic in a single individual of P. rockii. On an average, the pairwise marker difference between band profiles of conspecific individuals (different populations) was 7. 9 for P. suffruticosa subsp. spontanea and 8. 7 for P. rockii respectively. The average marker difference between P. suffruticosa subsp. spontanea and P. rockii was 10. 3. Obviously, greater number of plants and primers will be required to detect satisfactorily level of genetic diversity. These preliminary results showed that intraspecific genetic diversity was low for the two endangered species. RAPD as a molecular marker was useful and feasible for detecting the genetic variation within species of wild moutans. And it was also potential for studying evolution and relationships between species.
GC-MS analysis of essential oil of Paeonia suffruticosa Andrews from ZhaoFen and RouFurong flowers in China  [PDF]
Weiping Yin, Wenlu Duan, Pu Liu, Ruixue Deng, Yunlai Ren, Shuang Zhao
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.48073
Abstract: The flowers of ZhaoFen and RouFurong may contain essential oils with natural aromatic ingredients. In the present work, the chemical compositions of essential oil of Paeonia suffruticosa Andrews from the flowers of ZhaoFen and RouFurong grown only in China were investigated by GC-MS analysis. The results indicate that there are 27 constituents in ZhaoFen and 29 constituents in RouFurong, which account for 96.04% and 95.90% of the oils of ZhaoFen and RouFurong, respectively. The major components of the essential oils are character-rized by oxygenated terpenols, and their content is, respectively, 85.06% and 83.47%. The essential oil of Paeonia suffruticosa Andrews was reported for the first time on the aerial parts.
Notes on taxonomy of Paeonia sect. Moutan DC. (Paeoniaceae)
芍药属牡丹组分类新注

HONG De-Yuan,PAN Kai-Yu,
洪德元
,潘开玉

植物分类学报 , 2005,
Abstract: 自我们近年发表一系列牡丹组分类文章以来, 国内外基本上赞同我们8个种的分类系统,但对一些问题仍有不同见解.本文进一步申述牡丹Paeonia suffruticosa Andrews 是一个独立的种而不是人工杂种, 以及银屏牡丹P. suffruticosa ssp. yinpingmudan是牡丹P. suffruticosa的野生类型而不是逸生的P. ostii的理由.上述论点也得到了分子树的支持.P. jishanensis T. Hong & W. Z. Zhao是一个合法名称, 而P. spontanea (Rehder) T. Hong & W. Z. Zhao则确实是一个多余名.太白山紫斑牡丹的学名应是P. rockii ssp. atava (Brühl) D. Y. Hong & K. Y. Pan, 因此P. moutan Sims ssp. atava Brühl不应是可疑的分类群.本文还对Halda的6个组合和两个杂交种名作了处理.结果, 本文包括了5个新异名和一个新组合.
Notes on taxonomy of Paeonia sect. Moutan DC. (Paeoniaceae)
芍药属牡丹组分类新注

HONG De-Yuan,PAN Kai-Yu,
洪德元
,潘开玉

中国科学院研究生院学报 , 2005,
Abstract: 自我们近年发表一系列牡丹组分类文章以来, 国内外基本上赞同我们8个种的分类系统,但对一些问题仍有不同见解。本文进一步申述牡丹Paeonia suffruticosa Andrews 是一个独立的种而不是人工杂种, 以及银屏牡丹P. suffruticosa ssp. yinpingmudan是牡丹P. suffruticosa的野生类型而不是逸生的P. ostii的理由。上述论点也得到了分子树的支持。P. jishanensis T. Hong & W. Z. Zhao是一个合法名称, 而P. spontanea (Rehder) T. Hong & W. Z. Zhao则确实是一个多余名。太白山紫斑牡丹的学名应是P. rockii ssp. atava (Brühl) D. Y. Hong & K. Y. Pan, 因此P. moutan Sims ssp. atava Brühl不应是可疑的分类群。本文还对Halda的6个组合和两个杂交种名作了处理。结果, 本文包括了5个新异名和一个新组合。
Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway
Shih-Chin Wang,Sai-Wen Tang,Sio-Hong Lam,Chung-Chieh Wang,Yu-Huei Liu,Hsuan-Yuan Lin,Shoei-Sheng Lee,Jung-Yaw Lin
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/409823
Abstract: Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.
A STUDY ON POLLINATION BIOLOGY OF PAEONIA SUFFRUTICOSA SUBSP. SPONTANEA(PAEONIACEAE)
矮牡丹传粉生物学的初步研究

LUO Yi Bo,PEI Yan Long,PAN Kai Yu,HONG De Yuan,
罗毅波
,裴颜龙,潘开玉,洪德元

植物分类学报 , 1998,
Abstract: Field observation on pollination in three populations of Paeonia suffruticosa subsp. spontanea in southern Shanxi Province showed that five species of bees in two families and four species of beetles were engaged in pollination. Examination on bodies of these insects under SEM, and seed set produced by controlled insect pollination indicated that the bees, especially the species in the genus Andrena were main pollinators and the beetles except those of small size were only fluctuating vectors.Flowers of this taxon are scented and nonectariferous.Flowers attracted bees and beetles mainly by pollen. A study on seed set in the three populations revealed considerable variation within and among populations. The plants were no apomict and autogamy,but geitonogamy sometimes could produce mature seeds (x=2.86 seeds per carpel). In the population with less flowers (less than 40 flowers), natural seed set (x=3.88) was close to the seed set produced by hand cross pollination (x=3.21), but in the population with more flowers(near 100 flowers) natural seed set (x= 2.48 ) was lower than that by hand cross pollination (x=3.21). The seed set was low for both natural and artificial cross pollination with only about one forth of ovules developing into seeds.
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