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PRELIMINARY REPORT OF RAPD ANALYSIS IN PAEONIA SUFFRUTICOSA SUBSP.SPONTANEA AND PAEONIA ROCKII
矮牡丹与紫斑牡丹RAPD分析初报

Pel Yan-long Zou Yu-ping Yin Zhen Wang Xiao-quan Zhang Zhi-xian Hong De-yuan,Laboratory of Systematic,Evolutionary Botany,
裴颜龙
,邹喻苹

植物分类学报 , 1995,
Abstract: Ten different oligonucleotide primers of arbitrary sequence were used in Capillary and Air Thermocycler to amplify genomic total DNA of Paeonia suffruticosa subsp. spon-tanea and P. rockii isolated from several local populations in Shanxi, Shaanxi and Gansu provinces. Under the strictly standardized amplification condition for all the primers, these primers yielded clear and reproducible bands corresponding to amplified products and separable by agarose gel electrophoresis. Among a total of 71 bands amplified, 16(22. 5%) were polymorphic in a single individual of P. suffruticosa subsp. spontanea, while among a total of 76 bands 21(27. 6%)were polymophic in a single individual of P. rockii. On an average, the pairwise marker difference between band profiles of conspecific individuals (different populations) was 7. 9 for P. suffruticosa subsp. spontanea and 8. 7 for P. rockii respectively. The average marker difference between P. suffruticosa subsp. spontanea and P. rockii was 10. 3. Obviously, greater number of plants and primers will be required to detect satisfactorily level of genetic diversity. These preliminary results showed that intraspecific genetic diversity was low for the two endangered species. RAPD as a molecular marker was useful and feasible for detecting the genetic variation within species of wild moutans. And it was also potential for studying evolution and relationships between species.
Yinpingmudan, the Wild Relative of the King of Flowers, Paeonia suffruticosa Andrews
银屏牡丹——花王牡丹的野生近亲

HONG De-Yuan,PAN Kai-Yu,XIE Zhong-Wen,
洪德元
,潘开玉,谢中稳

中国科学院研究生院学报 , 1998,
Abstract: Paeonia suffruticosa Andrews subsp. spontanea (Rehder) S. G. Haw et L. A. Lauener ( =P. spontanea = P. jishanensis) has been considered as the wild form of widely cultivated P. suffruticosa. However, the subspecies differs from the cultivated one in shape, division and indumentum of leaves and reproductive biology. As a result of extensive field work, a form with two individuals in Yinping Shan, Caohu, Anhui, and Songxian, Henan, are found to be extremely similar to P. suffruticosa, but apparently different from P. jishanensis. The form is considered to be the wild one of P. suffruticosa and described as new: P. suffruticosa subsp, yinpingmudan Hong, K. Y. Pan et Z. W. Xie.
A Preliminary Study of the Chemistry and Systematics of Paeoniaceae
芍药科化学和系统学的初步研究

Yu Jin,Xiao Pei-Gen,
于津
,肖培根

中国科学院研究生院学报 , 1987,
Abstract: From the root of Paeonia suffruticosa and P. lactiflora seven compounds have been isolated. They are identified as paeoniflorin I, benzoylpaeoniflorin II, oxypaeoniflorin III, paeonol IV, paeonoside V, paeonolide VI and apiopaeonoside VII. The contents of the seven constituents in the root of 23 Paeonia taxa, including 19 species and 6 varieties, are determined by means of the quantitative HPTLC scanning method. The results show that paeoniflorin occurs ubiquitously in all the species examined and is the characteristic constituent of the family Paeoniaceae; The paeonol compounds (IV, V. VI, VII) are found to be restricted to the woody Sect. Moutan and notably absent in the herbaceous Sect. Paeon. This differentiation gives a support to the division of sections in Paeoniaceae. The chemical comparison between Paeoniaceae and 15 possibly related families shows that Paeoniaceae is different from Ranunculaceae, Ranunculales of Magnoliales and is similar to Dilleniaceae, Theaceae and Rosaceae in some respects. The chemical data support the sugges- tion that Paeoniaceae be elevated to the order rank, Paeoniales.
Notes on taxonomy of Paeonia sect. Moutan DC. (Paeoniaceae)
芍药属牡丹组分类新注

HONG De-Yuan,PAN Kai-Yu,
洪德元
,潘开玉

植物分类学报 , 2005,
Abstract: 自我们近年发表一系列牡丹组分类文章以来, 国内外基本上赞同我们8个种的分类系统,但对一些问题仍有不同见解.本文进一步申述牡丹Paeonia suffruticosa Andrews 是一个独立的种而不是人工杂种, 以及银屏牡丹P. suffruticosa ssp. yinpingmudan是牡丹P. suffruticosa的野生类型而不是逸生的P. ostii的理由.上述论点也得到了分子树的支持.P. jishanensis T. Hong & W. Z. Zhao是一个合法名称, 而P. spontanea (Rehder) T. Hong & W. Z. Zhao则确实是一个多余名.太白山紫斑牡丹的学名应是P. rockii ssp. atava (Brühl) D. Y. Hong & K. Y. Pan, 因此P. moutan Sims ssp. atava Brühl不应是可疑的分类群.本文还对Halda的6个组合和两个杂交种名作了处理.结果, 本文包括了5个新异名和一个新组合.
Notes on taxonomy of Paeonia sect. Moutan DC. (Paeoniaceae)
芍药属牡丹组分类新注

HONG De-Yuan,PAN Kai-Yu,
洪德元
,潘开玉

中国科学院研究生院学报 , 2005,
Abstract: 自我们近年发表一系列牡丹组分类文章以来, 国内外基本上赞同我们8个种的分类系统,但对一些问题仍有不同见解。本文进一步申述牡丹Paeonia suffruticosa Andrews 是一个独立的种而不是人工杂种, 以及银屏牡丹P. suffruticosa ssp. yinpingmudan是牡丹P. suffruticosa的野生类型而不是逸生的P. ostii的理由。上述论点也得到了分子树的支持。P. jishanensis T. Hong & W. Z. Zhao是一个合法名称, 而P. spontanea (Rehder) T. Hong & W. Z. Zhao则确实是一个多余名。太白山紫斑牡丹的学名应是P. rockii ssp. atava (Brühl) D. Y. Hong & K. Y. Pan, 因此P. moutan Sims ssp. atava Brühl不应是可疑的分类群。本文还对Halda的6个组合和两个杂交种名作了处理。结果, 本文包括了5个新异名和一个新组合。
银屏牡丹——花王牡丹的野生近亲
HONG De,Yuan,PAN Kai,Yu,XIE Zhong,Wen,
洪德元
,潘开玉,谢中稳

植物分类学报 , 1998,
Abstract: Paeonia suffruticosa Andrews subsp. spontanea (Rehder) S. G. Haw et L. A. Lauener (=P. spontanea=P. jishanensis) has been considered as the wild form of widely cultivated P. suffruticosa. However, the subspecies differs from the cultivated one in shape, division and indumentum of leaves and reproductive biology. As a result of extensive field work, a form with two individuals in Yinping Shan, Caohu, Anhui, and Songxian, Henan, are found to be extremely similar to P. suffruticosa, but apparently different from P. jishanensis. The form is considered to be the wild one of P. suffruticosa and described as new: P. suffruticosa subsp. yinpingmudan Hong, K. Y. Pan et Z. W. Xie.
Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
中国科学院研究生院学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。 在把作丹皮用的栽培牡丹(凤丹)描述为新种P. ostii后,观赏的栽培牡丹仍然被统称为P. suffruticosa。 但在P. suffruticosa复合体内, P. rockii, P. jishanensis, P. qiui 和P. suffruticosa ssp. yinpingmudan 等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P. suffruticosa “是牡丹组数
GC-MS analysis of essential oil of Paeonia suffruticosa Andrews from ZhaoFen and RouFurong flowers in China  [PDF]
Weiping Yin, Wenlu Duan, Pu Liu, Ruixue Deng, Yunlai Ren, Shuang Zhao
Natural Science (NS) , 2012, DOI: 10.4236/ns.2012.48073
Abstract: The flowers of ZhaoFen and RouFurong may contain essential oils with natural aromatic ingredients. In the present work, the chemical compositions of essential oil of Paeonia suffruticosa Andrews from the flowers of ZhaoFen and RouFurong grown only in China were investigated by GC-MS analysis. The results indicate that there are 27 constituents in ZhaoFen and 29 constituents in RouFurong, which account for 96.04% and 95.90% of the oils of ZhaoFen and RouFurong, respectively. The major components of the essential oils are character-rized by oxygenated terpenols, and their content is, respectively, 85.06% and 83.47%. The essential oil of Paeonia suffruticosa Andrews was reported for the first time on the aerial parts.
Sequential Analysis of Double Indexes of Common and Variant Peak Ratios in Ultraviolet Fingerprint of Paeonia Suffruticosa Andr. Root Bark
丹皮紫外指纹图谱共有峰率和变异峰率双指标序列分析

Yuan Jiurong,Yue Chunhu,Zou Huabin,Wang Aiwu,
袁久荣
,岳春华,邹华彬,王爱武

世界科学技术-中医药现代化 , 2004,
Abstract: Objective: To analyze the ultraviolet fingerprint of the root bark of Paeonia Suffruticosa Andr. and other medicinal crops of its kind by the method of sequential analysis of the double indexes of common and variant peak ratios in ultraviolet fingerprint. Method: In this article three solvents-Chloroform, absolute ethel alcohol and water are introduced to systematically extract the components of the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in different polar zones, examine their ultraviolet fingerprints and study the differences of the samples. Result: This method is able to accurately identify the root bark of Paeonia Suffruticosa Andr. and other medicinal plants of its kind in detail. Conclusion: Tow or several samples can be reliably identified by the method of sequential analysis of the double indexes of common and variaut peak ratios.
Paeonia suffruticosa Andrews 的界定,兼论栽培牡丹的分类鉴定问题
洪德元,潘开玉,周志钦
植物分类学报 , 2004,
Abstract: 栽培牡丹一直称为Paeonia suffruticosa Andrews。在把作丹皮用的栽培牡丹(凤丹)描述为新种P.ostii后,观赏的栽培牡丹仍然被统称为P.suffruticosa。但在P.suffruticosa复合体内,P.rockii,P.jishanensis,P.qiui和P.suffruticosa ssp.yinpingmudan等野生类型相继被描述并得到承认。于是,有人提出了栽培牡丹多元发生的观点,同时也出现了P.suffruticosa“是牡丹组数种植物复合体”的说法。本文在详细论述P.suffruticosa复合体分类历史的基础上,根据我们自己对野生类型和栽培类型的考察研究,认为观赏栽培牡丹的形成是多元的,并且包含几个物种;P.suffruticosa有明确的概念,它是观赏栽培牡丹的组成部分,而不代表栽培牡丹的全部,更不是数种植物的复合体。我们认为应根据形态特征正确鉴定观赏栽培牡丹,不应把它们一概鉴定为P.suffruticosa。通过文献和标本引证,文中界定了P.suffruticosa的范围。此外,把P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen作为P.suffruticosa ssp.yinpingmudan D.Y.Hong,K.Y.Pan&Z.W.Xie的异名,并且认定P.yinpingmudan(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen ssp.henanensis(D.Y.Hong,K.Y.Pan&Z.W.Xie)B.A.Shen是不合法名称。
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