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The effects of some factors on the diapause induction of Asian corn borer ( Ostrinia furnacalis )
亚洲玉米螟滞育诱导外源性因子研究

DAI Zhi Yi,
戴志一

生态学报 , 2000,
Abstract: This paper deals with the effects of food,temperature and photoperiod on the diapause of Asian corn borer, Ostrinia furnacalis (Güene'e).The results showed that the effects of different food on the diapause induction of Asian corn borer were significantly different.The sensitivity of diapause induction by photoperiod of the larvae fed by corn ear was stronger than that of the larvae fed by cotton boll.To some extent,higher concentration of soluble sugar in the food promoted the induction of larval diapau...
Identification of Immunity-Related Genes in Ostrinia furnacalis against Entomopathogenic Fungi by RNA-Seq Analysis  [PDF]
Yang Liu, Dongxu Shen, Fan Zhou, Guirong Wang, Chunju An
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086436
Abstract: Background The Asian corn borer (Ostrinia furnacalis (Guenée)) is one of the most serious corn pests in Asia. Control of this pest with entomopathogenic fungus Beauveria bassiana has been proposed. However, the molecular mechanisms involved in the interactions between O. furnacalis and B. bassiana are unclear, especially under the conditions that the genomic information of O. furnacalis is currently unavailable. So we sequenced and characterized the transcriptome of O. furnacalis larvae infected by B. bassiana with special emphasis on immunity-related genes. Methodology/Principal Findings Illumina Hiseq2000 was used to sequence 4.64 and 4.72 Gb of the transcriptome from water-injected and B. bassiana-injected O. furnacalis larvae, respectively. De novo assembly generated 62,382 unigenes with mean length of 729 nt. All unigenes were searched against Nt, Nr, Swiss-Prot, COG, and KEGG databases for annotations using BLASTN or BLASTX algorithm with an E-value cut-off of 10?5. A total of 35,700 (57.2%) unigenes were annotated to at least one database. Pairwise comparisons resulted in 13,890 differentially expressed genes, with 5,843 up-regulated and 8,047 down-regulated. Based on sequence similarity to homologs known to participate in immune responses, we totally identified 190 potential immunity-related unigenes. They encode 45 pattern recognition proteins, 33 modulation proteins involved in the prophenoloxidase activation cascade, 46 signal transduction molecules, and 66 immune responsive effectors, respectively. The obtained transcriptome contains putative orthologs for nearly all components of the Toll, Imd, and JAK/STAT pathways. We randomly selected 24 immunity-related unigenes and investigated their expression profiles using quantitative RT-PCR assay. The results revealed variant expression patterns in response to the infection of B. bassiana. Conclusions/Significance This study provides the comprehensive sequence resource and expression profiles of the immunity-related genes of O. furnacalis. The obtained data gives an insight into better understanding the molecular mechanisms of innate immune processes in O. furnacalis larvae against B. bassiana.
亚洲玉米螟的寄主植物种类
Host plants of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera:Crambidae)
 [PDF]

袁志华,王文强,王振营,何康来,白树雄,Yuan Zhihua,Wang Wenqiang,Wang Zhenying,He Kanglai,Bai Shuxiong
- , 2015, DOI: 10.13802/j.cnki.zwbhxb.2015.06.014
Abstract: 为探明亚洲玉米螟Ostrinia furnacalis (Guenée)的寄主植物种类,对13科38种植物进行了田间笼罩接虫试验和室内生物测定,并通过组建实验种群生命表分析了不同寄主植物对亚洲玉米螟生长发育和繁殖的影响。田间试验结果表明,亚洲玉米螟寄主植物有8科20种,酸模叶蓼是最适宜寄主植物,其次为玉米、谷子、薏苡等;在酸模叶蓼、玉米等11种植物上还发现了第2代幼虫。室内试验结果表明,在室内饲养条件下,亚洲玉米螟能在9科25种寄主植物上完成生活史,其中以向日葵为寄主植物的亚洲玉米螟种群增长最快,其次为玉米。研究表明,亚洲玉米螟的寄主植物共有9科27种,较适宜寄主植物有酸模叶蓼、玉米、谷子、苍耳和葎草,其中在玉米上的生长发育和繁殖最佳。
In order to explore the host species of Asian corn borer, Ostrinia furnacalis (Guenée), 38 plant species in 13 families were assessed with field experiment and laboratory bioassay. The experimental population life table was established to study the effects of different host plants on the development and fecundity of O. furnacalis. The field experiment results showed that 20 plant species in eight families were the host plants of O. furnacalis. Polygonum lapathifolium was the most suitable host plant, followed by Zea mays, Setaria italica, Coix lacroyma-jobi and so on. The second generation of O. furnacalis larvae was found on 11 plant species, such as P. lapathifolium, Z. mays and so on. The laboratory experiment results demonstrated that O. furnacalis could complete life history on 25 plant species in nine families under the indoor conditions. O. furnacalis population fed on Helianthus annuus grew fastest, followed by Z. mays. The study indicated that 27 plant species in nine families were the host plants of O. furnacalis. The suitable host plants were P. lapathifolium, Z. mays, Xanthium sibiricum, Abutilon theophrasti, and Humulus scandens, among which Z. mays was the most suitable host plant for the growth, development and fecundity of O. furnacalis.
短时极端高温对亚洲玉米螟卵、初孵幼虫和成虫的影响
Effects of transient exposure to extremely high temperatures on egg, neonate and adult of Ostrinia furnacalis
 [PDF]

全玉东,何康来,王振营,魏洪义,Quan Yudong,He Kanglai,Wang Zhenying,Wei Hongyi
- , 2015, DOI: 10.13802/j.cnki.zwbhxb.2015.06.018
Abstract: 为探明短时极端高温对亚洲玉米螟Ostrinia furnacalis (Guenée)的影响,采用耐热能力测定方法,研究了亚洲玉米螟经6 h高温处理后的半数致死温度(LT50)、存活率以及不同高温下的半数致死时间(Lt50)。结果表明,经6 h高温处理后,亚洲玉米螟卵、初孵幼虫和成虫的LT50分别为40.24~40.44、38.59和42.84~44.54℃;当卵、初孵幼虫、1日龄和3日龄成虫分别在37、35、41和37℃时,其存活率与对照均显著差异,而分别在39、37、43和39℃时,其存活率显著低于对照,卵、初孵幼虫、1日龄和3日龄成虫的短时高温阈值分别为37~39、35~37、41~43和37~39℃;当温度高于高温阈值时,卵、初孵幼虫和成虫的Lt50分别为0.38~6.31、0.22~8.35和3.80~24.15 h,且雄成虫的Lt50小于雌成虫。研究表明,短时极端高温对亚洲玉米螟的存活率具有显著影响。
To understand the effects of the transient exposure to extremely high temperatures on Asian corn borer, Ostrinia furnacalis (Guenée), the half lethal temperature (LT50) and the survival rate with 6 h high-temperature exposure, and the half lethal time (Lt50) under different high temperatures were examined. The results showed that the LT50 values of egg, neonate and moth were 40.24-40.44, 38.59 and 42.84-44.54℃, respectively. The survival rates of egg, neonate, 1-day moth and 3-day moth had no significant difference from the control at 37, 35, 41, or 37℃, respectively. However, the survival rates of egg, neonate, 1-day moth and 3-day moth were significantly lower than the control at 39, 37, 43 and 39℃, respectively. The critical high temperatures for egg, neonate, 1-day moth and 3-day moth were 37-39, 35-37, 41-43 and 37-39℃, respectively. Over the critical high temperature, the Lt50 values of egg, neonate and moth were 0.38-6.31, 0.22-8.35 and 3.80-24.15 h, respectively. The Lt50 of male was shorter than that of the female. The results indicated that extremely high temperatures significantly influenced the survival rate of O. furnacalis.
硒对亚洲玉米螟生长发育和生殖行为的影响
Effects of selenium on the development and reproductive behavior of Asian corn borer Ostrinia furnacalis(Guenée)
 [PDF]

韩晓霞,郑丽霞,魏洪义,王广利,Han Xiaoxia,Zheng Lixia,Wei Hongyi,Wang Guangli
- , 2017, DOI: 10.13802/j.cnki.zwbhxb.2017.2015170
Abstract: 为探讨硒(selenium)对亚洲玉米螟Ostrinia furnacalis(Guenée)的营养作用及其生长发育、生殖行为的影响,选用添加不同浓度硒的人工饲料饲喂3龄幼虫,观察了蛹、成虫的生长发育及成虫生殖行为情况。结果表明,不同浓度的硒对蛹和成虫的发育影响差异较大,其中硒浓度为0.75 mg/kg时生长发育最好,化蛹率、雄蛹体重和雄蛾寿命显著高于对照,分别提高了7.4%、12.7%和19.1%;硒浓度为5.00 mg/kg时生长发育最差,化蛹率和羽化率最低,雌、雄蛹重最小,成虫寿命最短。当硒浓度为0.75 mg/kg时,雌蛾求偶百分率和求偶持续时间均有所降低,且求偶高峰期推迟1~2 h。以正常雌蛾为诱源时,硒雄蛾的定向能力显著低于对照,而以硒雌蛾为诱源时,硒雄蛾的定向能力显著高于对照雄蛾;硒雌蛾的产卵量和卵孵化率显著提高,硒雌蛾×硒雄蛾组合提高最多,分别为28.9粒和9.3%。研究表明,适量硒可以促进亚洲玉米螟生长发育,并能显著影响成虫的生殖行为。
In order to explore nutritional effects of selenium on the Asian corn borer Ostrinia furnacalis(Guenée) and its relationship with O.furnacalis development and reproductive behaviors in the laboratory,the 3rd-instar larvae were fed by the artificial diet with different concentrations of selenium,and the growth,development and reproductive behaviors of O.furnacalis were observed.The results showed that the effects of different concentrations of selenium on the development of pupae and adults were different.The average pupation rate,male pupa weight and adult duration were significantly higher than that of the control at the concentration of 0.75 mg/kg,which were improved by 7.4%,12.7% and 19.1%,respectively.The O.furnacalis treated by selenium at the concentration of 5.00 mg/kg had the lowest pupation rate and emergence rate,the smallest pupal weight and the shortest adult longevity among different treatments.Furthermore,at an appropriate concentration of 0.75 mg/kg,the female had lower courtship percentage and duration than the control,and the courtship peak time was delayed by 1-2 h.Compared to the control,the male treated by selenium had significantly lower orientation to the control female,but significantly higher orientation to the female treated with selenium.Moreover,the fecundity and egg hatching rates of females treated by selenium significantly increased,especially in SeACB pairs,which laid 28.9 more eggs than the control,and the emergence rate increased by 9.3%.The results indicated that the proper amount of selenium could promote development of O.furnacalis,and significantly influence reproductive behaviors.
Variation in Courtship Ultrasounds of Three Ostrinia Moths with Different Sex Pheromones  [PDF]
Takuma Takanashi,Ryo Nakano,Annemarie Surlykke,Haruki Tatsuta,Jun Tabata,Yukio Ishikawa,Niels Skals
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0013144
Abstract: Moths use ultrasounds as well as pheromones for sexual communication. In closely related moth species, variations in ultrasounds and pheromones are likely to profoundly affect mate recognition, reproductive isolation, and speciation. The European corn borer, Ostrinia nubilalis, and its Asian congeners, Ostrinia furnacalis and Ostrinia scapulalis, exhibit within-species and between-species variation in their pheromone communication. Recently, we reported ultrasound communication in O. furnacalis; however, variations in ultrasounds in the three congeners have not been addressed to date. Here we investigated features of ultrasound production and hearing in O. nubilalis and O. scapulalis, and compared them with those of O. furnacalis. As in O. furnacalis, males of O. nubilalis and O. scapulalis produced ultrasounds during courtship by rubbing specialized scales on the wings against scales on the thorax. The covering of these scales with nail polish muffled the sounds and significantly reduced mating success in O. nubilalis, showing the importance of ultrasound signaling in mating. The ultrasounds produced by O. nubilalis and O. scapulalis were similar, consisting of long trains of pairs of pulses with a main energy at 40 kHz, but distinctly different from the ultrasound produced by O. furnacalis, consisting of groups of pulses peaking at 50 kHz and with substantially more energy up to 80 kHz. Despite overall similarities, temporal features and patterns of amplitude modulation differed significantly among the geographic populations of O. nubilalis and O. scapulalis, which differed in pheromone type. In contrast, no significant difference in hearing was found among the three species with regard to the most sensitive frequencies and hearing threshold levels. The patterns of variations in the songs and pheromones well reflected those of the phylogenetic relationships, implying that ultrasound and pheromone communications have diverged concordantly. Our results suggest that concordant evolution in sexual signals such as courtship ultrasounds and sex pheromones occurs in moths.
Molecular and Biochemical Characterization of a Novel β-N-Acetyl-D-Hexosaminidase with Broad Substrate-Spectrum from the Aisan Corn Borer, Ostrinia Furnacalis
Fengyi Liu, Tian Liu, Mingbo Qu, Qing Yang
International Journal of Biological Sciences , 2012,
Abstract: Insect β-N-acetyl-D-hexosaminidases with broad substrate-spectrum (IBS-Hex) are the homologues of human β-N-acetyl-D-hexosaminidase A/B (HsHex A/ B). These enzymes are distributed in most insect species and vary in physiological roles. In this study, the gene encoding an IBS-Hex, OfHEX2, was cloned from the Asian corn borer, Ostrinia furnacalis. Recombinant OfHex2 was expressed in Pichia pastoris and purified to homogeneity. By structure-based sequence alignment, three sequence segments with high diversity among IBS-Hexs were firstly concluded. Furthermore, the residue pair N423-R424/ D452-L453 important for the specificity of human β-N-acetyl-D-hexosaminidase subunits α/β toward charged/ non-charged substrates was not conserved in OfHex2 and other IBS-Hexs. Unlike HsHex A, OfHex2 could not degrade charged substrates such as 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide, ganglioside GM2 and peptidoglycan. OfHex2 showed a broad substrate-spectrum by hydrolyzing β1-2 linked N-acetyl-D-glucosamines from both α3 and α6 branches of biantennary N-glycan and β1-4 linked GlcNAc from chitooligosaccharides as well as β1-3 linked or β1-4 linked N-acetyl-D-galactosamine from oligosaccharides of glycolipids. Real-time PCR analysis demonstrated that the expression of OfHEX2 was up-regulated in the intermolt stages (both larva and pupa), and mainly occurred in the carcass rather than in the midgut during the feeding stage of fifth (final) instar larva. This study reported a novel IBS-Hex with specific biochemical properties, suggesting biodiversity of this class of enzymes.
Encapsulation of implanted foreign bodies by haemocytes in Ostrinia furnacalis larvae
亚洲玉米螟幼虫血细胞对外源体的包囊

LU Jian-feng,HU Jian,CHEN Xue-xin,ZHU Xiang-xiong,FU Wen-jun,
陆剑锋
,胡建,陈学新,朱湘雄,符文俊

浙江大学学报(农业与生命科学版) , 2007,
Abstract: Encapsulation by haemocytes in Ostrinia furnacalis larvae was biphasic. When DEAE-Sephadex beads were introduced into the haemocoele of O. furnacalis larvae, granular cells first attached to the surface of the beads within 3 minutes, which was followed by the attachment of multiple layers of plasmatocytes, eventually forming a smooth capsule comprised of overlapping layers of cells until 48 h post injection. The complete capsule showed three distinct regions. The role of the initial identification of foreign bodies within the haemocoele was played by the granular cells. Once the beads were identified as foreign bodies by granular cells, some putative materials released by lysed granular cells would induce plasmatocytes to recognize the foreign bodies, and then encapsulation was immediately triggered. Collectively, these results suggested that encapsulation in O. furnacalis larvae obviously depends on a cooperative response between haemocytes.
吉林省不同地区亚洲玉米螟发生世代的变化
Generation distribution change of the Asian corn borer Ostrinia furnacalis (Guenée) in Jilin Province
 [PDF]

鲁新,周淑香,李丽娟,陈立玲,常雪,张国红,Lu Xin,Zhou Shuxiang,Li Lijuan,Chen Liling,Chang Xue,Zhang Guohong
- , 2015, DOI: 10.13802/j.cnki.zwbhxb.2015.06.017
Abstract: 为明确吉林省不同地区亚洲玉米螟发生世代的变化,对吉林省东部、中部和西部3个生态区28个县(市)的亚洲玉米螟成虫产卵时期、幼虫发育和化蛹等情况进行了系统调查。结果显示,东部生态区大部分地区亚洲玉米螟只有1个落卵高峰期,未发现第1代幼虫化蛹,仍为1代发生区,而在伊通县和集安市有部分幼虫化蛹,已由1代发生区变为1代为主兼1.5代发生区;中部生态区大部分地区有2个落卵高峰期,未发现第2代幼虫化蛹,已由1.5代发生区变为完全2代发生区,而在双辽市和梨树县出现第3代低龄幼虫,已变为2代为主兼2.5代发生区;西部生态区大部分地区有2个落卵高峰期,未发现第2代幼虫化蛹,仍为2代发生区,而在通榆县出现第3代低龄幼虫,已由2代发生区变为2代为主兼2.5代发生区。表明随着全球气候变暖,吉林省亚洲玉米螟的世代发生数有增加的趋势。
To clarify the generation distribution changes of the Asian corn borer, Ostrinia furnacalis (Guenée), in different areas of Jilin Province, the adult oviposition period and larval development of O. furnacalis were systematically investigated in 28 counties in the eastern, central, and western ecoregions of Jilin Province. The results showed that the egg deposition period of eastern ecoregion had one peak period, and the generation number of eastern ecoregion was still one, while pupae were observed in Yitong and Ji'an, and the generation numbers of Yitong and Ji'an were one or one and a half; the egg deposition period of central ecoregion had two peak periods, and the generation number of central ecoregion had changed from one and a half to two, while the third generation larvae were observed in Shuangliao and Lishu, and the generation numbers of Shuangliao and Lishu were two or two and a half; the egg deposition period of western ecoregion had two peak periods, and the generation number of western ecoregion was still two, while the third generation larvae were observed in Tongyu, and the generation numbers of Tongyu were two or two and a half. The results indicated that the generation numbers of O. furnacalis in Jilin Province showed an increasing trend with global warming.
Application of High-Resolution DNA Melting for Genotyping in Lepidopteran Non-Model Species: Ostrinia furnacalis (Crambidae)  [PDF]
FengBo Li, BaoLong Niu, YongPing Huang, ZhiQi Meng
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029664
Abstract: Development of an ideal marker system facilitates a better understanding of the genetic diversity in lepidopteran non-model organisms, which have abundant species, but relatively limited genomic resources. Single nucleotide polymorphisms (SNPs) discovered within single-copy genes have proved to be desired markers, but SNP genotyping by current techniques remain laborious and expensive. High resolution melting (HRM) curve analysis represents a simple, rapid and inexpensive genotyping method that is primarily confined to clinical and diagnostic studies. In this study, we evaluated the potential of HRM analysis for SNP genotyping in the lepidopteran non-model species Ostrinia furnacalis (Crambidae). Small amplicon and unlabeled probe assays were developed for the SNPs, which were identified in 30 females of O. furnacalis from 3 different populations by our direct sequencing. Both assays were then applied to genotype 90 unknown female DNA by prior mixing with known wild-type DNA. The genotyping results were compared with those that were obtained using bi-directional sequencing analysis. Our results demonstrated the efficiency and reliability of the HRM assays. HRM has the potential to provide simple, cost-effective genotyping assays and facilitates genotyping studies in any non-model lepidopteran species of interest.
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