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Identification and Characterization of the Duffy Antigen Receptor for Chemokines (DARC) Gene in Sichuan Golden Monkey (Rhinopithecus roxellanae)
Xiao-Hui Si,Yong-Fang Yao,Shuan-Ling Zhang,Wei Liu,Liang Zhou,Huai-Liang Xu
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.4465.4471
Abstract: The Duffy Antigen Receptor for Chemokines (DARC) gene also called Duffy or FY, Plasmodium vivax and Plasmodium knowlesi use DARC to trigger internalization into red blood cells and cause malaria, the malaria life cycle in humans and nonhuman primates. In order to investigate DARC gene in golden snub-nosed monkey (Rhinopithecus roxellanae), two pairs of primers were designed based on DARC gene sequence of the Macaca mulatta (HQ285849.1) and used to amplify approximately 1.0 and 1.1 kb DNA fragments respectively by PCR technique from genomic DNA sample of golden monkey. The DNA, sequencing and combing results showed that the DARC gene of the golden monkey was 1593 bp in length and contained a 47 bp 5 flanking region, two extrons (21 and 990 bp), one complete intron (478 bp) and a 57 bp 3 flanking region. The Open Reading Fragment (ORF) was 1011 bp and encoded 336 amino acid residues. The DARC was a hydrophobic protein with less hydrophilic components. The prediction of topological structure for the protein indicated that it contained 16 potential function sites: three N-glycosylation sites, one protein kinase C phosphorylation site, two casein kinase II phosphorylation sites and ten N-myristoylation sites. In addition, the protein comprised seven transmembrane helix regions and four extracellular regions and four intracellular regions. Alignment analysis revealed that the homologies of DARC gene nucleotide sequence of golden snub-nosed monkey with other primate species and human was 95-99% and the homologies of amino acid sequence was 80-99%. These results would provide the molecular basis for golden monkey against human malarias.
Gazing Behavior of Rhinopithecus roxellanae in Qinling Mountains, China

- , 2017, DOI: 10.11984/j.issn.1000-7083.20160224
Abstract: 中文摘要:婴儿出生9个月就开始跟随母亲的视线接触外界社会活动,那么与人亲缘关系较近的灵长类,它们是否也拥有这种能力?凝视行为是否可以作为一种认知发育的行为指标?是否可以被测量并用于诊断?本研究选取秦岭川金丝猴 Rhinopithecus roxellanae为观察对象,设置川金丝猴照片引导实验,运用焦点动物取样法对不同年龄组的凝视行为进行观察记录。结果发现秦岭川金丝猴存在凝视现象,不同年龄组的行为发生方式存在差异,表明凝视行为可能是灵长类在早期就出现的一种发育行为。此研究方法可以为不同成长环境儿童的行为测量和诊断提供参考。
英文摘要:Human babies are able to follow their mother's line of sight at nine months of age. However, whether and when closely-related non-human primates exhibit this ability remains unclear. In addition, whether gazing behavior can be used as an indicator of cognitive development or measured and used for diagnosis are also important, but as yet unanswered questions. We studied a group of Rhinopithecus roxellanae in the Guanyinshan Nature Reserve in the southern Qinling Mountains of Foping county in China from March 2015 to February 2016. Using a photo guide experiment, we observed and recorded the behavior of different age groups. Results showed that gazing behavior in R. roxellanae existed, and the patterns of this behavior differed in different age groups, suggesting that gazing might be a developmental behavior in early stage. This research could provide a reference for the behavioral measurement and diagnosis of children under different growth environments. 2017,36(1): 30-33 收稿日期:2016-08-19 DOI:10.11984/j.issn.1000-7083.20160224 分类号:Q959.8 基金项目:陕西省科学院基金项目(2016K-20);国家重点自然科学基金项目(31130061);国家自然科学基金项目(31572278;31470214);陕西省自然科学基金项目(2016JM3016);西部资源生物与现代生物技术教育部重点实验室开放基金项目(ZS14002);日本Cosmo石油环保卡基金项目(2005-2016) 作者简介:孟子行,男,高三,E-mail:314439821@qq.com *通讯作者:赵海涛,E-mail:1986zhaohaitao@163.com 参考文献: 齐瓦·孔达著, 周治金, 朱新秤, 等译. 2013. 社会认知[M]. 北京:人民邮电出版社. 邵志芳, 高旭辰. 2009. 社会认知[M]. 上海:上海人民出版社. 石甲武. 2013. 别说你懂小孩子——我们该怎样陪着孩子一起成长[M]. 北京:人民邮电出版社. 张东君, 唐唐. 2014. 动物行为学的另类观察[M]. 北京:中信出版社. 张鹏. 2012. 猴、猿、人:思考人性的起源[M]. 广州:中山大学出版社. 赵海涛, 王晓卫, 李保国, 等. 2015. 秦岭南坡川金丝猴的食物组成及季节性变化[J]. 兽类学报, 35(2):130-137. Br?uer J, Call J, Tomasello M. 2005. All great ape species follow gaze to distant locations and around barriers[J]. Journal of Comparative Psychology, 119(2):145-154. Hauser MD, Glynn D, Wood J. 2007. Rhesus monkeys correctly read the goal-relevant gestures of a human agent[J].Proceedings of the Royal Society B Biological Sciences, 274(1620):1913-1918. Horton KE, Caldwell CA. 2006. Visual co-orientation and expectations about attentional orientation in pileated gibbons (Hylobates pileatus)[J]. Behavioural Processes, 72(1):65-73. Ittyerah M, Gaunet F. 2009. The response of guide dogs and pet dogs (Canis familiaris) to cues of human referential communication (pointing and gaze)[J]. Animal Cognition, 12(2):257-265. Itakura

ZHANG Li-Hong,ZHANG Wei-Min,CHENG Jia,ZHANG Yang,WU Jin-Ying,

水生生物学报 , 2007,
Abstract: 大海马(Hippocampus kudaBleeker)隶属鱼纲海龙目,是一种名贵的海产中药材,也是重要的海洋保护鱼类。我们通过快速扩增cDNA末端(rapid amplification of cDNA ends,RACE)的方法,首次从大海马的垂体总RNA中扩增出其垂体糖蛋白激素α亚基(pituitary glycoprotein hormoneαsubunit,PGHα)全长cDNA,其cDNA全长741bp(不包括polyA尾),5′和3′非编码区分别含有76和314bp,开放读码框为351bp,编码117aa的PGHα亚基前体(包括23aa的信号肽和94aa的成熟肽)。和其他脊椎动物一样,大海马PGHα亚基含有10个半胱氨酸残基,2个脯氨酸残基和2个N-糖基化位点。序列分析发现,大海马PGHα亚基的成熟肽与其他物种的同源性为50%—61.7%,其中与鲈形目和合鳃目的同源性最高(61.7%),而其信号肽与其他物种的同源性仅为8.7%—39.1%左右。这些结果表明大海马与已知的脊椎动物PGHα亚基同源性都比较低,是一种比较独特的鱼类。大海马PGHα亚基的全长cDNA克隆对海马垂体糖蛋白激素的结构和功能研究以及海马的养殖和保护有着重要的意义。
cDNA cloning and sequencing of ostrich Growth hormone
Doosti Abbas,Dehkordi Ghasemi Payam,Khodabakhsh Hosein
Archives of Biological Sciences , 2012, DOI: 10.2298/abs1202445d
Abstract: In recent years, industrial breeding of ostrich (Struthio camelus) has been widely developed in Iran. Growth hormone (GH) is a peptide hormone that stimulates growth and cell reproduction in different animals. The aim of this study was to clone and sequence the ostrich growth hormone gene in E. coli, done for the first time in Iran. The cDNA that encodes ostrich growth hormone was isolated from total mRNA of the pituitary gland and amplified by RT-PCR using GH specific PCR primers. Then GH cDNA was cloned by T/A cloning technique and the construct was transformed into E. coli. Finally, GH cDNA sequence was submitted to the GenBank (Accession number: JN559394). The results of present study showed that GH cDNA was successfully cloned in E. coli. Sequencing confirmed that GH cDNA was cloned and that the length of ostrich GH cDNA was 672 bp; BLAST search showed that the sequence of growth hormone cDNA of the ostrich from Iran has 100% homology with other records existing in GenBank.
川金丝猴(Rhinopithecus roxellanae)月经周期促黄体素(LH)的分泌水平

Yang Keqin,

动物学研究 , 1990,
Abstract: 本工作用改进的LH体外生物测定法,测定了一只川金丝猴两个月经周期的晨尿LH含量。结果表明LH峰分别出现在各月经周期的第16天和第11天。LH峰的平均水平为100.0±9.0 miu/ml尿以人促黄体素(hLH)69/104作为标准],是滤泡期LH平均水平的6倍,黄体期LH平均水平的4倍。提示川金丝猴月经周期LH的分泌情况与人和猕猴的极为相似。
The Spectrum of Hormone Immunoreactivity in Typical and Atypical Pituitary Adenomas  [cached]
Türk Patoloji Dergisi , 2009,
Abstract: Objective: We aimed to assess the spectrum of hormone immunoreactivity in our pituitary adenoma cases and discuss the diagnostic parameters of atypical pituitary adenomas.Material and Methods: A total of 166 pituitary adenoma cases diagnosed from 2005 to 2008 in our department were included in the present study. Hematoxylin-eosin stained and immunohistochemistry performed slides (ACTH, PRL, GH, TSH, FSH, LH, Ki-67, and p53) were evaluated. Cases having more than two mitoses on 10 high power fields besides more than 3% Ki-67 index were accepted in the atypical group.Results: Histologically, 159 cases were typical pituitary adenoma and 7 were atypical pituitary adenoma. Of the atypical pituitary adenoma cases, one case was ACTH, one GH and one both GH and prolactin hormone immunoreactive pituitary adenomas. Four cases were hormone immunonegative adenomas. Of the typical pituitary adenoma cases, 39 cases were GH, 19 ACTH, 17 prolactin, 10 FSH, 8 LH and one TSH immunreactive pituitary adenomas. Fourty-seven cases were hormone immunonegative adenomas.Twenty-two of the all pitutary adenoma cases had recurrence. Of these cases, 18 were typical adenoma and four were atypical adenoma.Conclusion: The ratio of prolactin immunoreactive pituitary adenoma cases in the surgical material of neuropathology is decreasing due to medical therapy. Atypical pituitary adenomas are not the sole factor affecting the recurrence mechanism but these tumors have higher recurrence rate compared with typical pituitary adenomas and we think the proliferation index might be the principal approach in the diagnosis of these lesions.
Three Japanese patients with congenital pituitary hormone deficiency and ophthalmological anomalies
Kuniko Takanashi,Yashuto Suzuki,Ayumu Noro,MInako Sugiyama
Pediatric Reports , 2011, DOI: 10.4081/pr.2011.e20
Abstract: The clinical phenotype of congenital pituitary hormone deficiency is variable and can be associated with a number of structural abnormalities of the central nervous system. We report three Japanese patients with congenital pituitary hormone deficiency and ophthalmological anomalies. Two of the patients initially showed strabismus and unilateral optic nerve hypoplasia. Thereafter, growth failure became evident, leading to the diagnosis of pituitary hormone deficiency. The other patient had severe congenital hypopituitarism with respiratory distress and hypoglycemia from the first day of life. In addition, he had prolonged jaundice and impaired liver function with bilateral optic nerve hypoplasia. Neuroimaging of the pituitary region in all three patients demonstrated a small anterior pituitary lobe and no pituitary stalk. Our findings indicate that clinical variability of congenital hypopituitarism must be considered. In a patient with ophthalmological symptoms, endocrine evaluation and neuroimaging of the CNS including the pituitary region should be considered.
Gonadotropin-inhibitory hormone action in the brain and pituitary  [PDF]
Takayoshi Ubuka,You Lee Son,Yasuko Tobari,Kazuyoshi Tsutsui
Frontiers in Endocrinology , 2012, DOI: 10.3389/fendo.2012.00148
Abstract: Gonadotropin-inhibitory hormone (GnIH) was first identified in the Japanese quail as a hypothalamic neuropeptide inhibitor of gonadotropin secretion. Subsequent studies have shown that GnIH is present in the brains of birds including songbirds, and mammals including humans. The identified avian and mammalian GnIH peptides universally possess an LPXRFamide (X = L or Q) motif at their C-termini. Mammalian GnIH peptides are also designated as RFamide-related peptides from their structures. The receptor for GnIH is the G protein-coupled receptor 147 (GPR147), which is thought to be coupled to Gαi protein. Cell bodies of GnIH neurons are located in the paraventricular nucleus (PVN) in birds and the dorsomedial hypothalamic area (DMH) in mammals. GnIH neurons in the PVN or DMH project to the median eminence to control anterior pituitary function. GPR147 is expressed in the gonadotropes and GnIH suppresses synthesis and release of gonadotropins. It was further shown in immortalized mouse gonadotrope cell line (LβT2 cells) that GnIH inhibits gonadotropin-releasing hormone (GnRH) induced gonadotropin subunit gene transcriptions by inhibiting adenylate cyclase/cAMP/PKA-dependent ERK pathway. GnIH neurons also project to GnRH neurons in the preoptic area, and GnRH neurons express GPR147 in birds and mammals. Accordingly, GnIH may inhibit gonadotropin synthesis and release by decreasing the activity of GnRH neurons as well as directly acting on the gonadotropes. GnIH also inhibits reproductive behavior possibly by acting within the brain. GnIH expression is regulated by a nocturnal hormone melatonin and stress in birds and mammals. Accordingly, GnIH may play a role in translating environmental information to inhibit reproductive physiology and behavior of birds and mammals. Finally, GnIH has therapeutic potential in the treatment of reproductive cycle and hormone-dependent diseases, such as precocious puberty, endometriosis, uterine fibroids, and prostatic and breast cancers.
Pituitary hormone circadian rhythm alterations in cirrhosis patients with subclinical hepatic encephalopathy  [cached]
Dimitrios Velissaris, Menelaos Karanikolas, Andreas Kalogeropoulos, Ekaterini Solomou, Panagiotis Polychronopoulos, Konstantinos Thomopoulos, Chrissoula Labropoulou-Karatza
World Journal of Gastroenterology , 2008,
Abstract: AIM: To analyze pituitary hormone and melatonin circadian rhythms, and to correlate hormonal alterations with clinical performance, hepatic disease severity and diagnostic tests used for the detection of hepatic encephalopathy in cirrhosis.METHODS: Twenty-six patients with cirrhosis were enrolled in the study. Thirteen patients hospitalized for systemic diseases not affecting the liver were included as controls. Liver disease severity was assessed by the Child-Pugh score. All patients underwent detailed neurological assessment, electroencephalogram (EEG), brain magnetic resonance imaging (MRI), assays of pituitary hormone, cortisol and melatonin, and complete blood chemistry evaluation.RESULTS: Pituitary hormone and melatonin circadian patterns were altered in cirrhosis patients without clinical encephalopathy. Circadian hormone alterations were different in cirrhosis patients compared with controls. Although cortisol secretion was not altered in any patient with cirrhosis, the basal cortisol levels were low and correlated with EEG and brain MRI abnormalities. Melatonin was the only hormone associated with the severity of liver insufficiency.CONCLUSION: Abnormal pituitary hormone and melatonin circadian patterns are present in cirrhosis before the development of hepatic encephalopathy. These abnormalities may be early indicators of impending hepatic encephalopathy. Factors affecting the human biologic clock at the early stages of liver insufficiency require further study.
Pituitary Stalk Interruption Syndrome: Diagnostic Delay and Sensitivity of the Auxological Criteria of the Growth Hormone Research Society  [PDF]
Géraldine Gascoin-Lachambre,Raja Brauner,Laetitia Duche,Martin Chalumeau
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0016367
Abstract: To study the diagnostic delay for pituitary stalk interruption syndrome (PSIS) with growth hormone deficiency (GHD) and the sensitivity of the auxological criteria of the Growth Hormone Research Society (GHRS) consensus guidelines.
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