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Antibacterial activity of some powdered herbal preparations marketed in Kaduna metropolis
D Abba, H.I Inabo, S.E Yakubu, OS Olonitola
Science World Journal , 2009,
Abstract: The aim of the study was to investigate the phytochemical components and the antibacterial activities of some powdered herbal medicinal preparations sourced from identified herbal shops and retail outlets in different parts of Kaduna metropolis. Extracts obtained from the herbal preparations were screened for the presence of secondary metabolites using established procedures. Also, antibacterial activities of the extracts were evaluated. Carbohydrates and tannins were identified in 105 (70%) and 101 (67.3%) of the samples respectively. Alkaloids were found in 97 (64.7%); saponins were detected in 91 (60.7%), while anthraquinones, flavonoids and cardiac glycosides were identified in 82 (54.7%), 80 (53.3%) and 60 (40%) of the herbal preparations respectively. All the methanolic extracts had inhibitory activities on the test bacterial isolates at various minimum inhibitory concentrations: 81 (54%) had inhibitory effects on Staphylococcus aureus, 74 (49.3%) on Escherichia coli, 74 (49.3%) on Salmonella typhi and 63 (42%) on Shigella spp. The uses of these products in herbal medicine are justified. However, further works are needed to identify the chemical nature of the active substances as well as their modes of actions on the bacterial cells and their roles in disease curing.
ASSESSMENT OF MICROBIAL CONTAMINATION IN COMMERCIAL HERBAL ORAL MEDICINAL LIQUIDS.  [cached]
Chitrarekha Kulkarni
International Journal of Pharmaceutical Research and Development , 2010,
Abstract: Worldwide demand for therapeutic herbal and neutraceutical preparations has increased greatly in past few years. In India, like other pharmaceutical preparations, there is a need to put strict regulations over the microbial quality of such preparations since they are consumed internally and safety is of prime concern. In this work we have focused on assessing the microbial quality of few marketed herbal liquid oral preparations. These preparations were procured from retail pharmacy outlets and traditional medicine sales outlets in western Maharashtra (India). None of the test samples could comply with the safety limits prescribed by WHO. This fact certainly cannot be ignored, and thus there is an immense need to prescribe and follow stringent regulations regarding microbiological quality of such herbal preparations.
The use of herbal preparations for tick control in western Ethiopia  [cached]
A. Regassa
Journal of the South African Veterinary Association , 2012, DOI: 10.4102/jsava.v71i4.722
Abstract: Information on the traditional tick control methods used in Keffa, Illubabor and Wellega Provinces in western Ethiopia was obtained from 86 veterinary clinics and 865 peasant farmers through a questionnaire survey. Latexes of Euphorbia obovalifolia and Ficus brachypoda, juice of crushed leaves of Phytolaca dodecandra and Vernonia amygdalina, fruit juice of Solanum incanum, crushed seeds of Lepidium sativum mixed with fresh cattle faeces, juice of crushed leaves and bark of Calpurnea aurea and commercially available spice of Capsicum spp. mixed with butter, were used by peasant farmers to control ticks. Preliminary in vitro efficacy tests of these plant preparations were performed on engorged female Boophilus decoloratus. Preparations of Capsicum spp., E. obovalifolia, S. incanum and F. brachypoda were found to have 30-100 % killing effects. Subsequently, in vivo treatment trials of these preparations were conducted using indigenous Bos indicus cattle naturally infested with ticks. Results indicate that treatments at the rate of once per day for 5 consecutive days with the latexes of E. obovalifolia and F. brachypoda can reduce tick burdens by up to 70 % on cattle.
Some Ghanaian herbal blood tonics as sources of Iron and other trace elements(Cu, Zn, Mn, Cd, Pb)
E Adei, L Nunoo, E Yankey
Journal of Science and Technology (Ghana) , 2009,
Abstract: Iron deficiency anaemia constitutes about 80 percent of all anaemia cases in developing countries. In Ghana, the 2003 Demographic and Health Survey reported anaemia prevalence of 67% for urban residents. Anaemia and loss of appetite are the common indications included in the consumer information on the labels on bottles of the numerous Ghanaian herbal blood tonics sold in retail pharmacy and chemical shops. In this study, ten brands of commonly advertised herbal blood tonics on the Ghanaian market were assessed for the levels of iron and other trace elements, wavelength of maximum absorption ( max), pH, salinity and total dissolved solids (TDS). These physico-chemical parameters were determined using a UNICAM 929 atomic absorption spectrophotometer, CECIL 8000 uv-visible spectrophotometer, JENWAY 3020 pH meter and LF538 conductivity meter. Maximum estimated daily dosages of less than 1 mg/day of iron was obtained for all the herbal tonics, except the Madam Catherine brand which had 2.17 mg, compared with the required daily intake of 10 – 15 mg. The tonics also appear to be poor sources of the essential metals determined. Cd was detected in Adutwumwaa, Madam Catherine and Top tonics, while Pb was detected in Amingya iron tonic and Madam Catherine; but the levels of these toxic metals will not exceed the recommended safety standards, if the manufacturer’s dosages are adhered to.
Use of certain herbal preparations in broiler feeds - A review  [cached]
Tirupathi Reddy Eevuri,Ramya Putturu
Veterinary World , 2013, DOI: 10.5455/vetworld.2013.172-179
Abstract: The importance and use of herbal remedies (turmeric, tulsi, amla and aloe vera etc.) has been reviewed systematically. Turmeric (curcumin), acts as an antioxidant, antimutagenic, antiinflamatory and antimicrobial agent and protect liver against a variety of toxicants. Tulsi (eugenol) have anticancer properties, reduced blood glucose levels, total cholesterol levels and promotes immune system function. Amla, richest source of vitamin-c and it's active tannoid principles have antimicrobial, antidiabetics, anticarcinogenic properties and enhances immune property. Aloe vera contains phytochemicals (Saponins, flavonoids, alkaloids and phenols), which is an indication of cosmetic and medicinal value. Turmeric, tulsi, amla and aloe vera preparations increased the body weight gain, feed efficiency and decreased the feed intake. These preparations decreased the mortality rates and the cost of feed has been decreased from 6.2% to 13.5%. They have reduced the fat accumulation, increased dressing percentage, liver weight, spleen weight and whole giblet weights. Significant reduction of serum cholesterol, serum triglycerides and increased the humoral response against RD vaccine. [Vet World 2013; 6(3.000): 172-179]
Development and Validation of a Microbiological Agar Assay for Determination of Orbifloxacin in Pharmaceutical Preparations  [PDF]
Edith C. L. Cazedey,Hérida R. N. Salgado
Pharmaceutics , 2011, DOI: 10.3390/pharmaceutics3030572
Abstract: Orbifloxacin is a fluoroquinolone with broad-spectrum antimicrobial activity, and belongs to the third generation of quinolones. Regarding the quality control of medicines, a validated microbiological assay for determination of orbifloxacin in pharmaceutical formulations has not as yet been reported. For this purpose, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method to quantify orbifloxacin in tablet formulations. The assay is based on the inhibitory effect of orbifloxacin upon the strain of Staphylococcus aureus ATCC 25923 used as test microorganism. The results were treated statistically by analysis of variance and were found to be linear ( r = 0.9992) in the selected range of 16.0–64.0 μg/mL, precise with relative standard deviation (RSD) of repeatability intraday = 2.88%, intermediate precision RSD = 3.33%, and accurate (100.31%). The results demonstrated the validity of the proposed bioassay, which allows reliable orbifloxacin quantitation in pharmaceutical samples and therefore can be used as a useful alternative methodology for the routine quality control of this medicine.
Microbiological Quality of Various Medicinal Herbal Teas and Coffee Substitutes
V. H. Tournas and E. J. Katsoudas
Microbiology Insights , 2012,
Abstract: Various herbal teas including German chamomile, Chrysanthemum Vascuflow herb tea, hop, jasmine and orange flowers, sweet marjoram, spearmint and thyme leaves, and papaya-mint tea as well as coffee substitutes (Bambu instant Swiss, Teeccino chocolate-mint, and Teeccino Mediterranean Espresso) were analyzed for fungal contamination and the presence of aerobic mesophilic bacteria (APC). The results of this investigation showed that fungal counts reached levels as high as 5.8 × 105 colony forming units (cfu) per gram. German chamomile harbored the highest fungal contamination. The most common fungi found in herbal teas were Aspergillus niger, Penicillium spp., Eurotium rubrum, E. chevalieri, A. flavus, Fusarium spp., Alternaria alternata, and yeasts. Among the coffee substitutes, only the chocolate-mint coffee was contaminated with low numbers (<1.0 × 103 cfu g 1) of E. rubrum, Ulocladium spp. and Phoma spp., and with yeasts (<100–6.8 × 103 cfu g 1). Aerobic mesophilic bacteria were recovered from 100% of the herbal tea, chocolate-mint and Mediterranean Espresso, and from 50% of the Bambu instant Swiss coffee samples. The highest APC counts of 1.2 × 107 cfu g 1 were observed in spearmint leaves.
Microbiological Quality of Various Medicinal Herbal Teas and Coffee Substitutes
V.H. Tournas,E.J. Katsoudas
Microbiology Insights , 2008,
Abstract: Various herbal teas including German chamomile, Chrysanthemum Vascuflow herb tea, hop, jasmine and orange flowers, sweet marjoram, spearmint and thyme leaves, and papaya-mint tea as well as coffee substitutes (Bambu instant Swiss, Teeccino chocolate-mint, and Teeccino Mediterranean Espresso) were analyzed for fungal contamination and the presence of aerobic mesophilic bacteria (APC). The results of this investigation showed that fungal counts reached levels as high as 5.8 × 105 colony forming units (cfu) per gram. German chamomile harbored the highest fungal contamination. The most common fungi found in herbal teas were Aspergillus niger, Penicillium spp., Eurotium rubrum, E. chevalieri, A. flavus, Fusarium spp., Alternaria alternata, and yeasts. Among the coffee substitutes, only the chocolate-mint coffee was contaminated with low numbers (<1.0 × 103 cfu g 1) of E. rubrum, Ulocladium spp. and Phoma spp., and with yeasts (<100–6.8 × 103 cfu g 1). Aerobic mesophilic bacteria were recovered from 100% of the herbal tea, chocolate-mint and Mediterranean Espresso, and from 50% of the Bambu instant Swiss coffee samples. The highest APC counts of 1.2 × 107 cfu g 1 were observed in spearmint leaves.
In Vitro Antioxidant Properties, HIV-1 Reverse Transcriptase and Acetylcholinesterase Inhibitory Effects of Traditional Herbal Preparations Sold in South Africa  [PDF]
Ashwell R. Ndhlala,Jeffrey F. Finnie,Johannes Van Staden
Molecules , 2010, DOI: 10.3390/molecules15106888
Abstract: The antioxidant potentials for fourteen multipurpose traditional herbal preparations sold in South Africa were determined using the DPPH radical scavenging, ferric reducing power and β-carotene-linoleic acid model system, the anti-HIV-1 reverse transcriptase (RT) enzyme inhibitory effects using an ELISA kit and acetylcholinesterase (AChE) enzyme inhibition using the microtitre plate assay. Nine of the herbal mixtures (Umzimba omubi, Umuthi wekukhwehlela ne zilonda, Mvusa ukunzi, Umpatisa inkosi, Imbiza ephuzwato, Vusa umzimba, Supreme one hundred, Sejeso herbal mixture Ingwe? and Ingwe? special muti) exhibited higher antioxidant potentials, while only four (Imbiza ephuzwato, Ingwe? muthi mixture, Sejeso herbal mixture Ingwe? and African potato extractTM) showed potent activity against the RT enzyme. Nine mixtures (Imbiza ephuzwato, Umpatisa inkosi, African potato extractTM, Sejeso herbal mixture Ingwe?, Vusa umzimba; Ingwe? muthi mixture, Ibhubezi?, Lion izifozonke Ingwe? and Ingwe? special muti) showed AChE enzyme inhibitory activity greater than 50%. The observed activity exhibited by some of the herbal mixtures gives some credence to the manufacturers’ claims and goes part of the way towards validating their use against certain conditions such as oxidative stress, HIV/AIDS proliferation and some mental conditions. It is however, desirable to carry out further studies to determine the effects of mixing plant species/parts in one mixture on the antioxidant potency as well as isolating active constituents from the herbal mixtures.
Development and Validation of a Combined Methodology for Assessing the Total Quality Control of Herbal Medicinal Products – Application to Oleuropein Preparations  [PDF]
Nikolaos Lemonakis, Evagelos Gikas, Maria Halabalaki, Alexios-Leandros Skaltsounis
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078277
Abstract: Oleuropein (OE) is a secoiridoid glycoside, which occurs mostly in the Oleaceae family presenting several pharmacological properties, including antioxidant, cardio-protective, anti-atherogenic effects etc. Based on these findings OE is commercially available, as Herbal Medicinal Product (HMP), claimed for its antioxidant effects. As there are general provisions of the medicine regulating bodies e.g. European Medicines Agency, the quality of the HMP’s must always be demonstrated. Therefore, a novel LC-MS methodology was developed and validated for the simultaneous quantification of OE and its main degradation product, hydroxytyrosol (HT), for the relevant OE claimed HMP’s. The internal standard (IS) methodology was employed and separation of OE, HT and IS was achieved on a C18 Fused Core column with 3.1 min overall run time employing the SIM method for the analytical signal acquisition. The method was validated according to the International Conference on Harmonisation requirements and the results show adequate linearity (r2 > 0.99) over a wide concentration range [0.1–15 μg/mL (n=12)] and a LLOQ value of 0.1 μg/mL, for both OE and HT. Furthermore, as it would be beneficial to control the quality taking into account all the substances of the OE claimed HMP’s; a metabolomics-like approach has been developed and applied for the total quality control of the different preparations employing UHPLC-HRMS-multivariate analysis (MVA). Four OE-claimed commercial HMP’s have been randomly selected and MVA similarity-based measurements were performed. The results showed that the examined samples could also be differentiated as evidenced according to their scores plot. Batch to batch reproducibility between the samples of the same brand has also been determined and found to be acceptable. Overall, the developed combined methodology has been found to be an efficient tool for the monitoring of the HMP’s total quality. Only one OE HMP has been found to be consistent to its label claim.
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