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Transcriptome Profiling of the Abdominal Skin of Larimichthys crocea in Light Stress Transcriptome Profiling of the Abdominal Skin of Larimichthys crocea in Light Stress  [PDF]
HAN Zhaofang,LV Changhuan,XIAO Shijun,YE Kun,ZHANG Dongling,TSAI Huai Jen,WANG Zhiyong
- , 2018,
Abstract: Large yellow croaker(Larimichthys crocea), one of the most important marine fish species in China, can change its abdominal skin color when it is shifted from light to dark or from dark to light, providing us an opportunity of investigating the molecular responding mechanism of teleost in light stress. The gene expression profile of fish under light stress is rarely documented. In this research, the transcriptome profiles of the abdominal skin of L. crocea exposed to light or dark for 0 h, 0.5 h and 2 h were produced by next-generation sequencing(NGS). The cluster results demonstrated that stress period, rather than light intensity(e.g., light or dark), is the major influencing factor. Differently expressed genes(DEGs) were identified between 0 h and 0.5 h groups, between 0 h and 2 h groups, between 0.5 h light and 0.5 h dark, and between 2 h light and 2 h dark, respectively. The gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) annotation revealed that the genes relating to immunity, energy metabolism, and cytoskeletal protein binding were significantly enriched. The detailed analysis of transcriptome profiles also revealed regular gene expression trends, indicating that the elaborate gene regulation networks underlined the molecular responses of the fish to light stress. This transcriptome analysis suggested that systematic and complicated regulatory cascades were functionally activated in response to external stress, and coloration change caused by light stress was mainly attributed to the change in the density of chromatophores for L. crocea. This study also provided valuable information for skin coloration or light stress research on other marine fish species
HERITABILITY AND TOLERANCE OF LARIMICHTHYS CROCEA TO ENVIRONMENTAL FACTORS
大黄鱼(Larimichthys crocea)耐环境因子试验及其遗传力的估计

WANG Xiao-Qing,WANG Zhi-Yong,HE Xiang-Rong,
王晓清
,王志勇,何湘蓉

海洋与湖沼 , 2009,
Abstract: Larimichthys crocea is an important economical fish inhabiting often in China, whose heritability is an important genetic character for preserving quality traits. In this study, test on its tolerability to low-salinity, low-oxygen and low-pH water in 15 semi-sib family of L. crocea, and heritability estimation on the tolerability was performed. Causal components of phenotypic variance were calculated with GLM (General Linear Mode1) module of SAS9.0 software. The results show that 40-day-old fries died entir...
Genome Sequencing of the Perciform Fish Larimichthys crocea Provides Insights into Molecular and Genetic Mechanisms of Stress Adaptation  [PDF]
Jingqun Ao?,Yinnan Mu?,Li-Xin Xiang?,DingDing Fan?,MingJi Feng?,Shicui Zhang?,Qiong Shi?,Lv-Yun Zhu?,Ting Li?,Yang Ding
PLOS Genetics , 2015, DOI: 10.1371/journal.pgen.1005118
Abstract: The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral and physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia and air exposure. These traits may render L. crocea a good model for investigating the response mechanisms to environmental stress. To understand the molecular and genetic mechanisms underlying the adaptation and response of L. crocea to environmental stress, we sequenced and assembled the genome of L. crocea using a bacterial artificial chromosome and whole-genome shotgun hierarchical strategy. The final genome assembly was 679 Mb, with a contig N50 of 63.11 kb and a scaffold N50 of 1.03 Mb, containing 25,401 protein-coding genes. Gene families underlying adaptive behaviours, such as vision-related crystallins, olfactory receptors, and auditory sense-related genes, were significantly expanded in the genome of L. crocea relative to those of other vertebrates. Transcriptome analyses of the hypoxia-exposed L. crocea brain revealed new aspects of neuro-endocrine-immune/metabolism regulatory networks that may help the fish to avoid cerebral inflammatory injury and maintain energy balance under hypoxia. Proteomics data demonstrate that skin mucus of the air-exposed L. crocea had a complex composition, with an unexpectedly high number of proteins (3,209), suggesting its multiple protective mechanisms involved in antioxidant functions, oxygen transport, immune defence, and osmotic and ionic regulation. Our results reveal the molecular and genetic basis of fish adaptation and response to hypoxia and air exposure. The data generated by this study will provide valuable resources for the genetic improvement of stress resistance and yield potential in L. crocea.
Parentage assignment and parental contribution analysis in large yellow croaker Larimichthys crocea using microsatellite markers  [cached]
Xiande LIU, Guangtai ZHAO, Zhiyong WANG, Mingyi CAI, Hua YE, Qiurong WANG
Current Zoology , 2012,
Abstract: The large yellow croaker Larimichthys crocea is one of the most important fish species in China. To estimate the reproductive success of breeders, three independent full-factorial crosses were created and the fins of breeders and progenies were sampled for microsatellite analysis. Out of 959 offspring from three sets, 99.6% were assigned to their parents using 6–7 microsatellite markers. In all crosses, some parent pairs produced a large number of offspring and some parent pairs did not produce any offspring. The contributions of male or female parents were unequal, ranging from 1.0–89.3% across the three sets. The loss of putative Ne was 69.6% in set 1, 31.2% in set 2 and 57.6% in set 3. These results suggest that the unequal contribution of parents is universal in artificial breeding of L. crocea, especially in a small population, and this should be taken into account in hatcheries or when releasing animals for resource enhancement [Current Zoology 58 (2): 244-249, 2012].
Family structure and phylogenetic analysis of odorant receptor genes in the large yellow croaker (Larimichthys crocea)
Yingsong Zhou, Xiaojun Yan, Shanliang Xu, Peng Zhu, Xianxing He, Jianxin Liu
BMC Evolutionary Biology , 2011, DOI: 10.1186/1471-2148-11-237
Abstract: Eighty-nine full-length and 22 partial OR cDNA sequences were isolated from the olfactory epithelium of the large yellow croaker, Larimichthys crocea. Bayesian phylogenetic analysis classified the vertebrate OR genes into two types, with several clades within each type, and showed that the L. crocea OR genes of each type are more closely related to those of fugu, pufferfish and stickleback than they are to those of medaka, zebrafish and frog. The reconciled tree showed 178 duplications and 129 losses. The evolutionary relationships among OR genes in these fishes accords with their evolutionary history. The fish OR genes have experienced functional divergence, and the different clades of OR genes have evolved different functions. The result of real-time PCR shows that different clades of ORs have distinct expression levels.We have shown about 100 OR genes to be expressed in the olfactory epithelial tissues of L. crocea. The OR genes of modern fishes duplicated from their common ancestor, and were expanded over evolutionary time. The OR genes of L. crocea are closely related to those of fugu, pufferfish and stickleback, which is consistent with its evolutionary position. The different expression levels of OR genes of large yellow croaker may suggest varying roles of ORs in olfactory function.Vertebrates can distinguish numerous odorants in the environment using chemosensory receptors that are expressed in the olfactory epithelium [1-3]. Four types of chemosensory receptors have been found in the vertebrate olfactory epithelium, including the main odorant receptors (ORs) [4], vomeronasal receptors (VRs) [5-7], trace-amine associated receptors (TAARs) [8] and formyl peptide receptor-like proteins [9]. The OR genes were initially identified in mouse olfactory organ by Linda Buck and Richard Axel [4], who found that each olfactory sensory neuron expressed a single OR allele [4,10,11]. The ORs, located on the surface of dendrites of sensory neurons on the olfactory epithel
Loss of Genetic Diversity in the Cultured Stocks of the Large Yellow Croaker, Larimichthys crocea, Revealed by Microsatellites  [PDF]
Le Wang,Xiaofeng Shi,Yongquan Su,Zining Meng,Haoran Lin
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13055584
Abstract: The large yellow croaker ( Larimichthys crocea) is the most important mariculture fish species in China and the wild stocks of this croaker have collapsed in the past decades due to high fishing pressure and habitat degradation. Due to a lack of wild croaker samples, however, studies concerning the genetic changes of the cultured croaker stocks compared to their wild counterparts were never conducted. Here, we collected three wild populations in the northern and central East China Sea during fisheries survey and investigated the differences in terms of genetic diversity and differentiation between and within cultured stocks and wild populations. Our results demonstrated that the cultured croaker had significantly reduced genetic diversity in contrast to the wild populations, and also presented statistically significant differentiation from the wild, indicating that enhancement of the current wild stock should be conducted with caution. These changes may be caused by founder effects, artificial selection and random genetic drift. With a relatively high level of genetic diversity, the wild populations showed important value for improving the ongoing breeding program of this croaker. Further, we detected no differentiation among the wild populations, suggesting that the wild croaker in the northern and central East China Sea should be considered as one unit for management and conservation.
大黄鱼(Larimichthys crocea)I型干扰素基因的特征与表达分析  [PDF]
李婵,姚翠鸾
海洋与湖沼 , 2013, DOI: 10.11693/hyhz201302037037
Abstract: 采用RT-PCR和RACE-PCR技术克隆了大黄鱼(Larimichthys crocea)I 型干扰素基因全长cDNA序列及其基因组序列, 并利用荧光定量PCR技术研究了该基因在大黄鱼不同组织中的表达谱, 以及副溶血弧菌(Vibrio parahaemolyticus)、LPS、poly I:C刺激后大黄鱼脾脏、肝脏和头肾组织中该基因在转录水平的表达变化。结果表明, 大黄鱼I型IFN基因全长cDNA为878bp, 开放阅读框558bp, 编码185个氨基酸。推测的大黄鱼I型IFN氨基酸序列N端含有20个氨基酸的信号肽, 其后包含一个保守的IFabd结构域。大黄鱼I型IFN基因包含5个外显子, 4 个内含子, 在大多数组织和细胞中都有表达, 其中在血细胞中表达量最高, 肌肉中表达量最低。Poly I:C刺激可诱导大黄鱼I型干扰素基因在脾脏、头肾和肝脏中的表达量显著上调; LPS刺激可诱导大黄鱼I型干扰素基因在脾脏和肝脏中的表达量显著上调; 灭活的副溶血弧菌可诱导大黄鱼I型干扰素基因在头肾和肝脏中的表达量显著上调。
Subcellular Localization of Large Yellow Croaker(Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response Subcellular Localization of Large Yellow Croaker(Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response  [PDF]
SUN Qingxue,FAN Zejun,YAO Cuiluan
- , 2018,
Abstract: Toll-like receptor 21(TLR21) is a non-mammalian type TLR, and plays an important role in innate immune response in fish. In this paper, the full-length cDNA sequence of TLR21 gene was identified and characterized from large yellow croaker, Larimichthys crocea and was termed as LcTLR21. It consists of 3365 bp, including a 5'-terminal untranslated region(UTR) of 97 bp, a 3'-terminal UTR of 331 bp, and an open reading frame(ORF) of 2937 bp encoding a polypeptide of 978 amino acid residues. The deduced LcTLR21 contains a signal peptide domain at N-terminal, 12 leucine-rich repeats(LRRs) at the extracellular region, a transmembrane domain and a cytoplasmic toll-interleukin-1 receptor(TIR) domain at the C-terminal. Subcellular localization analysis revealed that the LcTLR21-GFP was constitutively expressed in cytoplasm. Tissue expression analysis indicated that LcTLR21 gene broadly expressed in most of the examined tissues, with the most predominant abundance in spleen, followed by head-kidney and liver, while the weakest expression was detected in brain. The expression level of LcTLR21 after LPS, poly I:C and Vibrio parahaemolyticus challenges was investigated in spleen, head-kidney and liver. LcTLR21 gene transcripts increased significantly in all examined tissues after the challenges, and the highest expression level was detected in liver at 24 h after poly I:C stimulation(P <0.05), suggesting that LcTLR21 might play a crucial role in fish resistance to viral and bacterial infections
Expression Profile of Immune-Associated Genes in the Kidney of Cultured Large Yellow Croaker Larimichthys crocea in the East China Sea Area Expression Profile of Immune-Associated Genes in the Kidney of Cultured Large Yellow Croaker Larimichthys crocea in the East China Sea Area  [PDF]
ZHAO Shujiang,ZHAO Qian,CHEN Yinghua,LV Baoqiang,WU Xiongfei,LIU Huihui,ZHU Aiyi,WU Changwen
- , 2016,
Abstract: To explore the effect of environment conditions on immune activity of fish, eight immune-associated genes responsible for innate immunity were selected from the Gen Bank, i.e. Pgrn-a, Ifit2, P-hepcidin, Lect2, β2m, Irf1, Il25 and Hsp96, and the m RNA expressions of them in the kidney of cultured large yellow croaker Larimichthys crocea in different sea areas in the East China Sea were examined with q PCR techniques. In the contrasts of immune-associated gene expression between areas and populations, significant differences were found, expression levels of these immune-associated genes were lower in the clear water area than in the poor water quantity area, and lower in May than in October. MY was more sensitive to environmental factors than DQ, which was coincident with the water quality in the culturing areas. Differential analyses of the expression levels of these immune-associated genes showed that significant up-regulation could be triggered by poor environmental factors. The expression patterns indicated that the expression levels of these genes were sensitive to ecological changes, thereby the immune-associated genes, especially Pgrn-a, Ifit2, β2m, Il25 and Hsp96, might serve as immediate and sensitive indicators of population immunologic vigor and ecosystem health. But the expression of immunity-associated genes at the level of gene transcription is highly influenced by multiple factors, and the exact causes or influencing factors of the up-regulation or down-regulation of these genes still need further thorough investigation
Comparasion of some immune parameters between different strains of large yellow croaker (Larimichthys crocea)
大黄鱼选育群体与普通养殖群体部分免疫指标的比较

杨启莲,姚翠鸾,王志勇
海洋科学 , 2012,
Abstract: We determined some immune parameters between the selected strain named Minyou No.1 and common culture strain of large yellow croaker (Larimichthys crocea). The results showed that the activities of lysozyme and SOD in blood cells of the Minyou No.1 were significantly higher than those in the common group (P<0.05). While the immune globulin levels of Minyou No.1 was lower than that of the common group. Furthermore, a lower cumulative mortality was found in Minyou No.1 after artifically infected with Vibrio parahaemolyticus in 8 days, which suggested that non-specific immunity might play a more important role in the early stage of large yellow croaker defense against pathogenic-infection.
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