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7种林木植物对华北大黑鳃金龟取食和繁殖的影响
Effects of seven species of forest plants on feeding and fecundity of Holotrichia oblita
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刘子欢,陆秀君,宁文烁,李瑞军,赵丹,郭巍,Liu Zihuan,Lu Xiujun,Ning Wenshuo,Li Ruijun,Zhao Dan,Guo Wei
- , 2015, DOI: 10.13802/j.cnki.zwbhxb.2015.05.008
Abstract: 为明确华北大黑鳃金龟Holotrichia oblita (Faldermann)的嗜食植物,优化其室内人工饲养技术及为诱虫植物提供候选材料,以花生叶片为对照,研究了7种林木植物叶片对华北大黑鳃金龟取食和繁殖的影响。结果表明,华北大黑鳃金龟对8种植物日均取食量由高到低依次为金叶女贞、刺槐、核桃、榆树、花生、柳树、香椿、毛白杨,其中金叶女贞、香椿和毛白杨与花生间有极显著差异,金叶女贞与刺槐、核桃、榆树间显著差异;单雌产卵量由高到低依次为金叶女贞、柳树、核桃、香椿、花生、榆树、毛白杨、刺槐,其中金叶女贞、柳树、核桃、刺槐与花生间有极显著差异,而金叶女贞与柳树间显著差异。研究表明,饲养华北大黑鳃金龟成虫适宜饲料植物为金叶女贞,日均取食量最高为4.70 g,产卵期最长为56.00 d,单雌产卵量最多为58.88个,其次为核桃和柳树。
To clarify the feeding preferences of Holotrichia oblita (Faldermann), improve the indoor artificial breeding technology and provide materials for the trap plants, the influences of seven species of forest plants on feeding and fecundity of H. oblita with Arachis hypogaea L. as control were studied. The results showed that the average daily consumption of H. oblita to the leaves of the eight species plants were as followed: Ligustrumx vicaryi, Robinia pseucdoacacia, Juglans regia, Ulmus pumila, A. hypogaea, Salix matsudana, Toona sinensis and Populus tomentosa, in descending order. L. vicaryi, T. sinensis, P. tomentosa showed highly significant difference from the control, but the difference between R. pseucdoacacia, J. regia and U. pumila was not significant. The ovoposition per female of H. oblita were as followed: L. vicaryi, S. matsudana, J. regia, T. sinensis, A. hypogaea, U. pumila, P. tomentosa and R. pseucdoacacia, in descending order. L. vicaryi, S. matsudana, J. regia and R. pseucdoacacia showed highly significant difference from the control A. hypogaea, while S. matsudana had no highly significant difference from L. vicaryi. The results indicated that the leaves of L. vicaryi was the suitable feeding plant for H. oblita adults, slightly better than J. regia and S. matsudana, with a highest average daily consumption of 4.70 g, a longest spawning period of 56.00 d and a maximum fecundity per female of 58.88.
华北大黑鳃金龟聚集信息素的分离鉴定及其引诱效果
Identification and bioassay of aggregation pheromone components of northern China scarab beetle Holotrichia oblita
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李雪,李建一,曹雅忠,尹姣,兰建强,李克斌,Li Xue,Li Jianyi,Cao Yazhong,Yin Jiao,Lan Jianqiang,Li Kebin
- , 2018, DOI: 10.13802/j.cnki.zwbhxb.2018.2017163
Abstract: 为确定华北大黑鳃金龟Holotrichia oblita聚集行为产生的原因和是否存在聚集素,采用Y型嗅觉仪和气相色谱-质谱联用仪(gas chromatography-mass spectrometer,GC-MS),观测试虫聚集行为、鉴定后肠粗提物主要成分及其生物活性。结果表明,在华北大黑鳃金龟雄虫后肠粗提物中发现了顺-9-十八烯醛和乙酸顺-9-十八烯酯等活性物质;与对照相比,100 μg/mL和200 μg/mL浓度的顺-9-十八烯醛对华北大黑鳃金龟雌、雄成虫具有显著的引诱效果,100 μg/mL浓度引诱的雌、雄虫数分别为119头和102头,显著多于对照(分别为60头和75头);而100 μg/mL和50 μg/mL浓度的乙酸顺-9-十八烯酯浓度对雌、雄虫具有显著的引诱效果。当顺-9-十八烯醛与2-乙基-1-己醇混合比例分别为3∶1和5∶1时,华北大黑鳃金龟雌、雄虫对混合物的选择性显著高于对照。当乙酸顺-9-十八烯酯与榆树挥发物混合比例为3∶1、5∶1时,对华北大黑鳃金龟雌虫的引诱性显著高于对照;当混合比例为5∶1和7∶1时,对华北大黑鳃金龟雄虫的引诱显著高于对照。表明顺-9-十八烯醛和乙酸顺-9-十八烯酯可能是华北大黑鳃金龟聚集信息素的主要成分。
To examine the aggregation behavior of northern China scarab beetle Holotrichia oblita in the laboratory, and screen the potential intraspecific aggregation pheromone of this species, their aggregation behavior was observed, and the main components of crude extract and biological activity were identified by using Y-olfactometer and gas chromatography-mass spectrometer (GC-MS). The results showed that Z-9-octadecanoic aldehyde and Z-9-octadecene acetate and other active substances were found in the hindgut extracts of male, in which both female and male adults were significantly baited by Z-9-octadecanoic aldehyde at a rate of 100 μg/mL and 200 μg/mL compared with the control. At the rate of 100 μg /mL, the number of attractant insects of female and male were 119 and 102, respectively, which was significantly more than that of the control (60 and 75). Moreover, there was a significant attraction for both male and female when Z-9-octadecene acetate was presented at the concentrations of 100 μg/mL and 50 μg/mL. When mixed with the elm tree volatile (2-ethyl-1-hexanol) in different proportions, both male and female exhibited significant preference to the mixture of Z-9-octadecanoic aldehyde at the proportions of 3∶1 and 5∶1. When Z-9-octadecene acetate and the elm tree volatile were mixed at 3∶1 and 5∶1 ratio, there was a notable effect in seducing female. In addition, there was a significant attraction effect at 5∶1 and at 7∶1 ratio for male. The results suggested that Z-9-octadecanoic aldehyde and Z-9-octadecene acetate were probably the components of the aggregation pheromone of H. oblita.
Potential Cooperations between Odorant-Binding Proteins of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)  [PDF]
Bing Wang, Li Guan, Tao Zhong, Kebin Li, Jiao Yin, Yazhong Cao
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084795
Abstract: It was previously thought that the odorant binding proteins (OBPs) in the sensillum lymph might serve as carriers, which could carry lipophilic odorant molecules to olfactory receptors. In this study, two novel OBP genes of the scarab beetle Holotrichia oblita were screened using an antennal cDNA library. The full cDNA of HoblOBP3 and HoblOBP4 was cloned using reverse transcription PCR and rapid amplification of the cDNA ends. Homology modeling of both OBPs was performed using SWISS-MODEL on-line tools. Next, the two OBPs were expressed in Escherichia coli and purified using Ni ion affinity chromatography. The ligand-binding properties of HoblOBP3 and HoblOBP4 in 42 ligands respectively were measured using the fluorescence probe N-phenyl-naphthylamine (1-NPN). The results obtained from competitive binding assays demonstrated that HoblOBP4 showed a broader range of binding affinities to the test compounds, while HoblOBP3 displays more specific binding affinity. Furthermore, other OBPs and CSPs were expressed in Escherichia coli and purified using Ni ion affinity chromatography. Binding curves were measured for binary mixtures of OBPs and CSPs using 1-NPN, and the Scatchard plots exhibited “J”-like nonlinear correlation trends in some samples. In addition, competitive binding assays of the HoblOBP1 and HoblOBP2 mixtures and of the HoblOBP2 and HoblOBP4 mixtures with representative compounds unexpectedly demonstrated good affinity, which revealed extreme differences that were only obtained using the individual proteins. In the immunocytochemical analysis, colocalization of HoblOBP1 and HoblOBP2, and of HoblOBP2 and HoblOBP4, was detected in the sensilla basiconica and sensilla placodea, respectively. All of these results suggested that HoblOBP1 and HoblOBP2, as well as HoblOBP2 and HoblOBP4, may serve as heterodimers in the sensillum lymph.
对大黑鳃金龟甲幼虫高效的苏云金芽胞杆菌筛选及cry基因鉴定
Screening and identification of cry genes from Bacillus thuringiensis isolates highly toxic to the larvae of Holotrichia oblita
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王小奇,束长龙,蒋健,张风娇,刘春琴,张杰,Wang Xiaoqi,Shu Changlong,Jiang Jian,Zhang Fengjiao,Liu Chunqin,Zhang Jie
- , 2016, DOI: 10.13802/j.cnki.zwbhxb.2016.03.018
Abstract: 为获得对大黑鳃金龟甲幼虫具有较高杀虫活性的菌株,利用拌土法测定了本实验室分离的500株苏云金芽胞杆菌(Bacillus thuringiensis,Bt)对大黑鳃金龟甲的杀虫活性,建立Bt菌株比较鉴别技术来进行多样性分析,并对菌株的晶体形态及幼虫感染Bt后的中肠组织切片进行观察。结果显示,从500株Bt菌株中筛选到42株对大黑鳃金龟甲幼虫具有不同程度活性的菌株,分属于14个菌株类型;其中有10株校正死亡率大于60%,261-1菌株杀虫活性最高,7 d校正死亡率达100%。从14个菌株类型中各选取1个代表菌株进行基因鉴定,仅P65-1、1126-1、FCD114和78-2菌株分别含有cry8Ma、cry8Ca、cry8Ab、cry8Ga、cry8Ea共5个cry8类新基因,其它10个菌株均不含cry3、cry8、cry18、cry23、cry37、cry43等对鞘翅目害虫有效的杀虫基因。14个菌株中,78-3、127-2和1198-1菌株能产生双锥体型晶体,261-1、FCD114、P65-1、FTL84、78-2和1126-1菌株能产生球形晶体。幼虫感染261-1、1198-1、FCD114、1126-1、QDL40-2菌株后,中肠组织发生明显的病理变化:2 d时肠壁细胞明显出现空洞化,排列疏松,4 d时受到严重破坏并脱离底膜。表明筛选到的Bt菌株具有防治大黑鳃金龟甲幼虫的潜力。
In order to obtain Bacillus thuringiensis (Bt) isolates with higher insecticidal activity for the larvae of Holotrichia oblita, 500 Bt isolates in the laboratory were investigated; the diversity analysis method was developed, and the crystal shapes were observed in Bt isolates. Furthermore, the histopathological changes were observed in the midgut of H. oblita larvae infected by a mixture of crystals and spores produced by tested isolates by using histopathological slice. The results showed that 42 isolates were found toxic to larvae of this pest, and these isolates were classified into 14 different types, of which ten isolates had a corrected mortality of over 60%, and the isolate 261-1 had the highest toxicity reaching 100% in seven days after infection. One isolate was selected from each type of isolates to identify cry genes. The results indicated that four isolates, P65-1, 1126-1, FCD114 and 78-2, contained five novel cry8 genes, i.e. cry8Ma, cry8Ca, cry8Ab, cry8Ga and cry8Ea, and the other ten isolates, including the isolate 261-1, did not contain cry genes toxic to H. oblita larvae, such as cry3, cry8, cry18, cry23, cry37and cry43. Three isolates, 78-3, 127-2 and 1198-1, contained double pyramid-shaped crystals, and spherical crystals were observed in six isolates, 261-1, FCD114, P65-1, FTL84, 78-2 and 1126-1. Isolates 261-1, 1198-1, FCD114, 1126-1 and QDL40-2 could cause obvious histopathological changes in larval midgut. The midgut cells became loose arrangement and appeared with obvious vacuolization, and were seriously damaged and even deviated from the membrane. The Bt isolates screened in this study may have applications in biological control of H. oblita larvae.
Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)
Sauka, Diego Herman;Monella, Rodrigo Horacio;Benintende, Graciela Beatriz;
Pesquisa Agropecuária Brasileira , 2010, DOI: 10.1590/S0100-204X2010000400012
Abstract: the objective of this work was to establish and test the induced-feeding bioassay in order to detect bacillus thuringiensis insecticidal activity against epilachna paenulata (coleoptera: coccinellidae). larvae were induced to swallow high concentrations of spore-crystal suspensions of seven exotic and 30 argentine b. thuringiensis strains. the great majority of strains showed no toxicity to e. paenulata larvae, and observed mortality was lower than 30%. induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of cry proteins needed to an efficient pest control.
The effects of aqueous root extract of Senna italica subsp. arachoides on the feeding performance of Hyalomma marginatum rufipes adults
MK Thembo, SR Magano, LJ Shai
African Journal of Biotechnology , 2010,
Abstract: The effects of aqueous root extracts of Senna italica subsp. arachoides on the feeding performance of adults of Hyalomma marginatum rufipes in three consecutive infestations of rabbits were studied under laboratory conditions. Rabbits were divided into treatment group (n = 3), fed aqueous root extracts and rabbit pellets while the control group (n = 3) was fed with rabbit pellets and given distilled water only. Ticks that infested the treatment rabbits fed poorly, taking significantly longer feeding periods (P < 0.05) to acquire the bloodmeal, and having significantly reduced engorgement weights (P < 0.05) compared to those that fed on the control rabbits during the first and second infestations. These results suggest that aqueous root extracts of S. italica subsp. arachoides reduced the feeding performance of H. marginatum rufipes adults.
Flight, feeding and reproductive behavior of Phyllophaga cuyabana (Moser) (Coleoptera: Melolonthidae) adults
Oliveira, Lenita Jacob;Garcia, Maria Alice;
Pesquisa Agropecuária Brasileira , 2003, DOI: 10.1590/S0100-204X2003000200003
Abstract: phyllophaga cuyabana is a univoltine species and its development occurs completely underground. its control by conventional methods, such as chemical and biological insecticides, is difficult, so it is important to understand its dispersion, reproduction, and population behavior in order to determine best pest management strategies. the objective of this work was to study the behavior of adults of p. cuyabana. this study was carried out in the laboratory, greenhouse and field sites in paraná state, brazil (24o25' s and 52o48' w), during four seasons. the results obtained demonstrate that: a) p. cuyabana adults have a synchronized short-flight period when mating and reproduction occurs; b) adults tend to aggregate in specific sites for mating; c) the majority of adults left the soil on alternate nights; d) the choice of mating and oviposition sites was made by females before copulation, since after copulation adults did not fly from or bury themselves at nearby locations; e) females that fed on leaves after mating, oviposited more eggs than females that had not fed;f) plant species such as sunflower (helianthus annuus) and the crotalaria juncea are important food sources for adults.
Flight, feeding and reproductive behavior of Phyllophaga cuyabana (Moser) (Coleoptera: Melolonthidae) adults  [cached]
Oliveira Lenita Jacob,Garcia Maria Alice
Pesquisa Agropecuária Brasileira , 2003,
Abstract: Phyllophaga cuyabana is a univoltine species and its development occurs completely underground. Its control by conventional methods, such as chemical and biological insecticides, is difficult, so it is important to understand its dispersion, reproduction, and population behavior in order to determine best pest management strategies. The objective of this work was to study the behavior of adults of P. cuyabana. This study was carried out in the laboratory, greenhouse and field sites in Paraná State, Brazil (24o25' S and 52o48' W), during four seasons. The results obtained demonstrate that: a) P. cuyabana adults have a synchronized short-flight period when mating and reproduction occurs; b) adults tend to aggregate in specific sites for mating; c) the majority of adults left the soil on alternate nights; d) the choice of mating and oviposition sites was made by females before copulation, since after copulation adults did not fly from or bury themselves at nearby locations; e) females that fed on leaves after mating, oviposited more eggs than females that had not fed;f) plant species such as sunflower (Helianthus annuus) and the Crotalaria juncea are important food sources for adults.
Toxicity bioassays in marine sediments
Onelio Carballo Hondal,Gustavo Arencibia Carballo,Joel Concepción,Mercedes Isla Molleda
RETEL : Revista de Toxicología en Línea , 2010,
Abstract: Many organic and in organic contaminants originated from human activities are deposited and concentrated in aquatic sediments. The purpose of this review article is to analyze and discuss, the objectives, procedures and applications of bioassays on the triad of marine sediments, aiming at a reflection on the possibility of implementing regulations on the environmental monitoring of these studies in our country, so as to improve the environmental quality and its management on coastal zones. The main objective is to highlight the understanding of the adequate application and interpretation of bioassays. A summary of reports is presented, knowledge and analysis concerning marine sediments including, the chemical analysis, the direct determination of ecological changes, the toxicity bioassays and the lines of multiple evidence, the objectives of the toxicity test on sediments, the most important approaches and considerations of bioassays on whole sediments. One of the most convincing conclusions is the need of evaluating the environmental quality not only testing the chemistry of the chemistry of the sediment and structure of the benthic community, but incorporating the toxicity tests as a line of fundamental evidence on the triad of sediment quality.
3种金龟甲触角感器超微结构的比较
Ultrastructure Comparison of the Antennal Sensilla of Three Scarabs (Coleoptera:Scarabaeidae)
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,,,,张国云,陆俊娇,仵均祥,李怡萍
- , 2015, DOI: 10.7606/j.issn.1004-1389.2015.05.024
Abstract: 金龟甲类是危害严重的地下害虫,利用化学信息素可以有效监测与防治金龟甲。昆虫触角感器是感受外界信息素的主要器官。利用扫描电子显微镜对暗黑鳃金龟(Holotrichia parallela Motschulsky)、华北大黑鳃金龟(Holotrichia oblita Faldermann)和铜绿丽金龟(Anomala corpulenta Motschulsky)成虫的触角形态及感受器超微结构进行研究。结果表明,暗黑鳃金龟和华北大黑鳃金龟鞭节棒状部由5节组成,铜绿丽金龟由4节组成。暗黑鳃金龟触角感器有10种,华北大黑鳃金龟触角感器有8种,铜绿丽金龟触角感器有9种。3种金龟甲雌、雄个体之间触角感器的类型、分布均明显差异,但不同感器在触角各节上的数量存在差异。研究结果对科学理解金龟甲类害虫感知信息化合物有一定参考。
Scarabs are important underground pests.Scarabs have been effectively monitored and controlled using semiochemicals.Antennal sensillas of insect are the major organs sensing pheromone.of the outside.The antennae morphology and sensilla of Holotrichia parallela Motschulsky,Holotrichia oblita Faldermann and Anomala corpulenta Motschulsky were observed by scanning electron microscope.The results showed that the funicle of H.parallela Motschulsky and H.oblita Faldermann have 5 segments,whereas the funicle of A.corpulenta Motschulsky consists of 4 segments.The antennae of H.parallela Motschulsky contain 10 kinds of sensilla,8 types of sensilla on H.oblita Faldermann and 9 types of sensilla on A.corpulenta Motschulsky.No difference was found in the type and distribution of the sensilla between the male and female adults.The numbers of each sensillum,however,were different in different segments of the antennae.The results can provide some references for scientific understanding of the scarab beetle’s sense of semiochemicals .
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