oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
稀土硝酸盐对蚓蚯生长繁殖的影响  [PDF]
农业环境科学学报 , 1992,
Abstract: 70年代以来,稀土在我国作为一种微肥已逐渐在水稻、小麦、大豆等几十种农作物上应用。与此同时,稀土的毒性及其对农田生态环境的影响也引起了人们的关注。国内科学家对此做了研究,但稀土对环节动物蚯蚓影响的研究迄今尚未见报道。蚓蚯是腐食性土壤动物,它在生态系统的分解
Impact of Benzo[a]pyrene the Expression of Mitochondrion-encoded Genes in the Earthworm Eisenia fetida
苯并[a]芘对赤子爱胜蚓线粒体编码基因表达影响研究

ZHENG Sen-lin,SONG Yu-fang,QIU Xiao-yan,SUN Tie-heng,ZHANG Wei,ML Ackland,
郑森林
,宋玉芳,邱晓燕,孙铁珩,张薇,M.L. Ackland

环境科学 , 2008,
Abstract: The earthworm Eisenia fetida's benzo a] pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-Select cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunit I (CO I), subunit II (CO II), subunit Ill (CO III), NADH dehydrogenase subunit 1 (NDH1), and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure.
Detection of DNA Damage in Earthworm (Eisenia foetida )in Vivo Exposure to Copper Ion
铜暴露下赤子爱胜蚓(Eisenia foetida)活体基因的损伤研究

BU Yuan-qing,LUO Yong-ming,TENG Ying,LI Zhen-gao,
卜元卿
,骆永明,滕应,李振高

生态毒理学报 , 2006,
Abstract: 通过碱性单细胞凝胶电泳法研究了Cu2 暴露剂量对赤子爱胜蚓(Eiseniafoetida)活体基因损伤的动态影响.结果显示:不添加Cu2 的对照组和添加Cu2 的处理组蚯蚓体腔细胞尾部DNA含量和尾长均呈非正态分布(p<0.05);在暴露72h时,125mg·L-1Cu2 处理组尾部DNA含量值最大,为41.44%,100mg·L-1Cu2 处理组尾长值最大,为33.79μm;随着Cu2 暴露剂量的增加,尾部DNA含量和尾长损伤频率增加;对照组和处理组的尾部DNA含量和尾长之间均存在显著性差异(p<0.05),Spearman非参数相关分析表明,尾部DNA百分含量和尾长之间呈显著相关(p<0.01,n=21),Cu2 暴露浓度与尾部DNA百分含量、尾长具有良好的剂量-构效关系(p<0.01).在125mg·L-1Cu2 浓度下暴露72h时蚯蚓的基因损伤程度达到最大,损伤程度为3级.可见,蚯蚓DNA生物标志物是重金属污染基因毒理诊断的重要指标,碱性SCGE试验是检测Cu2 暴露对赤子爱胜蚓活体基因损伤的有效手段.
Acute Toxicity of Nickel to Earthworm Eisenia fetida in Typical Chinese Soils
不同类型土壤中外源镍对赤子爱胜蚓的急性毒性

Xie Dongli,Zhou Juan,Wang Beixin,Jiang Jinwei,Li Fasheng,Yan Zengguang,
解冬利
,周娟,王备新,蒋金炜,李发生,颜增光

生态毒理学报 , 2011,
Abstract: Acute toxicity of nickel to earthworm Eisenia fetida in 13 spiked soils was investigated using the methods of International Standardization Organization (ISO). The results show that nickel toxicity to E. fetida differs in different soils, and 50% lethal concentrations (14 LC50) varies from 243.8 to 1 970.6 mg kg-1. Nickel toxicity to E. fetida in a range of Chinese soils can be ranked as red soil (Hunan)>purple soil (Chongqing)>red earth (Jiangxi)>fluvo\|aquic soil (Henan)>black soil (Jilin)>paddy soil (Zhejiang)>grey desert soil (Xinjiang)>fluvo\|aquic soil (Beijing)>loessal soil (Shaanxi)>fluvo\|aquic soil (Shandong)>fluvo\|aquic soil (Hebei)>paddy soil (Jiangsu)>black soil (Heilongjiang). An eight\|fold difference of nickel LC50 exists between red soil (Hunan) and black soil (Heilongjiang). Stepwise multiple regressions are carried out to determine the relative contributions of some soil properties (pH, organic matter capacity, cation exchange content and clay content) to nickel LC50. Significant positive correlations were observed between soil pH, organic matter content and nickel LC50. Soil pH and organic matter content can account for 79.4% variance for nickel LC50. The ecotoxicity data generated in the current study may provide useful reference information for the development of ecological soil screening values in China.
Ecototoxicological Effect of ZnO and TiO2 Nanoparticles on Earthworm (Eisenia foetida)
纳米ZnO与TiO2对赤子爱胜蚓(Eisenia foetida)的 毒性效应

Hu Changwei,Cui Yibin,Li Dingsheng,Gao Xiangyu,Yang Liuyan,Li Mei,
胡长伟
,崔益斌,李丁生,高香玉,杨柳燕,李梅

生态毒理学报 , 2011,
Abstract: 以赤子爱胜蚓(Eisenia foetida )为受试生物,研究纳米TiO2与ZnO两种纳米颗粒的毒理学效应及环境释放风险。实验采用滤纸法(浓度组:0、0.15、0.75、1.5和3.0 mg·cm-2)和溶液法(浓度组:0、10、50和100 mg·L-1),染毒时间为48 h,以抗氧化系统的响应和DNA损伤作为毒理效应的评价指标。结果表明:0-3.0 mg ·cm-2浓度范围 内,纳米颗粒以滤纸法染毒48 h未引起蚯蚓死亡;浓度为1.5 mg·cm-2的纳米ZnO处理后,蚯蚓体内丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GPX)活性较空白组显著上升 (p<0.05),分别升高58.25%和43.30%;而对于TiO2处理组,过氧化氢酶(CAT)与GPX活性和MDA含量均出现低浓度促进和高浓度抑制的现象。溶液法中,经浓度为0-100 mg·L-1的TiO2或ZnO处理后,MDA和各抗氧化酶活性未出现明显的变化趋势(p>0.05),但彗星实验结果发现,分别以100 mg·L-1的TiO2和ZnO染毒48 h,蚯蚓均出现明显DNA损伤。实验结果说明:纳米TiO2与ZnO在较低浓度下对蚯蚓无明显毒性效应;在溶液法最高的实验浓度(100 mg·L-1)下,纳米ZnO对蚯蚓产生一定程度的毒性;DNA损伤可作为检测环境中纳米污染物对蚯蚓胁迫程度较为灵敏的指标。
Influence of Soil Temperature and Moisture on the Cocoon Production and Hatching of the Exotic Earthworm Pontoscolex corethrurus
土壤温度和湿度对外来种蚯蚓Pontoscolex corethrurus 产茧和幼蚓孵化的影响

ZHANG Hua,YANG Xiao-dong,DU Jie,WU Yi-xue,
张花
,杨效东,杜杰,吴艺雪

动物学研究 , 2008,
Abstract: 通过采用主处理为土壤温度,副处理为土壤湿度的裂区区组实验设计,在室内微生态条件下控制土壤温度和湿度培养蚯蚓,探讨了3种土壤温、湿度对热带外来种蚯蚓Pontoscolex corethrurus成蚓产茧和蚓茧孵化的影响。研究结果表明,P.corethrurus成蚓在20—30℃土壤温度范围、25%—35%土壤湿度环境中均可持续产茧和孵化幼蚓,培养时间、土壤温湿度对蚯蚓的产茧和孵化呈现显著影响。20%土壤湿度下成蚓休眠或死亡。35%土壤湿度下呈现最大蚓茧产量和幼蚓孵化量。在较好(35%)土壤湿度下产茧数表现为高温好于低温,反之亦然;在适宜(25%—35%)土壤湿度下幼蚓孵化率随温度升高而增加,但呈现高、低温的限制作用,即25℃土壤温度和35%土壤湿度时出现最高幼蚓孵化率。在适宜的土壤温湿度范围内,湿度较温度对蚯蚓繁殖具有更显著的控制作用,温度的影响在一定程度上可通过土壤湿度加以调节。
Earthworm Protease  [PDF]
Rong Pan,Zi-Jian Zhang,Rong-Qiao He
Applied and Environmental Soil Science , 2010, DOI: 10.1155/2010/294258
Abstract: The alimentary tract of earthworm secretes a group of proteases with a relative wide substrate specificity. In 1983, six isozymes were isolated from earthworm with fibrinolytic activities and called fibriniolytic enzymes. So far, more isozymes have been found from different earthworm species such as Lumbricus rubellus and Eisenia fetida. For convenience, the proteases are named on the basis of the earthworm species and the protein function, for instance, Eisenia fetida protease (EfP). The proteases have the abilities not only to hydrolyze fibrin and other protein, but also activate proenzymes such as plasminogen and prothrombin. In the light of recent studies, eight of the EfPs contain oligosaccharides chains which are thought to support the enzyme structure. Interestingly, EfP-II has a broader substrate specificity presenting alkaline trypsin, chymotrypsin and elastase activities, but EfP-III-1 has a stricter specificity. The protein crystal structures show the characteristics in their specificities. Earthworm proteases have been applied in several areas such as clinical treatment of clotting diseases, anti-tumor study, environmental protection and nutritional production. The current clinical utilizations and some potential new applications of the earthworm protease will be discussed in this paper. 1. Introduction Earthworm has been recorded with a long history. Five hundred years ago, Shizhen Li compiled the famous medical book Compendium of Material, in which the earthworm (Earth dragon) was recorded as a drug prescribed for antipyretic and diuretic purposes in the form of dried powder in clinic. Now the remedy is still used in the folk. In the end of 19th century, Frédéricq [1] discovered one enzyme secreted from the alimentary tract of earthworm. Then several proteases were separated from the earthworm in 1920 [2]. They could dissolve casein, gelatin, and albumin. This was the preliminary research about the earthworm proteases. Large-scale research about earthworm protease began in 1980. Mihara et al. [3] isolated a group of proteases with fibrinolytic activity from the earthworm Lumbricus rubellus. Subsequently different purification methods were applied to isolate the enzymes, including gel filtration, affinity chromatography, ion exchanging chromatography, and high-pressure liquid chromatography (HPLC). More proteases have been obtained from different species, such as lumbrokinase [4], earthworm-tissue plasminogen activator [5], earthworm plasminogen activator [6–11], component A of EFE (EFEa) [12, 13], and biologically active glycolipoprotein
Plant Hormones in Earthworm Casts and Their Promotion on Adventitious Root Formation of Mung Bean Cutting
蚓粪中的植物激素及其对绿豆插条不定根发生的促进作用

HU Pei,
胡佩

生态学报 , 2002,
Abstract: 利用高效液相色谱证实了环毛蚓(Pheretima sp.)蚓粪中至少存在两种植物激素(IAA和GA3),蚓粪中IAA和GA3的含量分别可达13.9mg/kg和49.9mg/kg。为原土的13.6和8.2倍,采用包括蚓粪提取液,原土提取液,Haogland营养液,蒸馏水,IAA,GA3等在内的11种供试液进行绿豆插条不定根发生的生物试验,结果表明,在相同条件下,蚓粪提取液处理的绿豆插条比其它处理提早3天出现不定根,统计证明,与原土提取液,Haogland营养液,蒸馏水等处理相比,蚓粪提取液处理可显提高绿豆插条不定根的数量,长度和生物量,蚓粪提取液对不定根发生数量的诱导效应与85-170μmol/L的IAA溶液相当,但从不定根根长和根生长量来看,蚓粪提取液的效果优于IAA。
Comparative Study on Toxicity Differences of Copper to Earthworm Eisenia fetida in Four Typical Soils
几种典型土壤中铜对赤子爱胜蚓的毒性差异比较研究

ZHOU Juan,YAN Zeng-guang,JIANG Jin-wei,TANG Qing-bo,LI Fa-sheng,
周娟
,颜增光,蒋金炜,汤清波,李发生

生态毒理学报 , 2008,
Abstract: 土壤污染物基础生态毒理数据是开展污染土壤生态风险评价和构建土壤生态筛选基准的重要依据.以湖南红壤、北京潮土、吉林黑土和经济合作与发展组织(OECD)推荐的人工土壤为测试介质,按照国际标准化组织(ISO)颁布的测试方法,研究了铜在4种不同土壤中对赤子爱胜蚓(Eisenia fetida)的急性和慢性毒性.结果表明,铜在4种不同类型土壤中对赤子爱胜蚓的毒性效应差异较大,在湖南红壤、北京潮土、吉林黑土和人工土壤中的半致死浓度(14dLC50)分别为134.4、557.7、715.2和867.0mg·kg-1;赤子爱胜蚓体重对较低浓度铜污染响应相对不敏感,而较高浓度铜污染(接近引起蚯蚓死亡的高浓度)则可导致蚯蚓体重显著降低;铜对赤子爱胜蚓生殖(产茧量)的影响较为明显,其在湖南红壤、北京潮土、吉林黑土和人工土壤中对蚯蚓产茧量产生50%抑制作用的有效中浓度(28d EC50)分别为58.2、140.1、258.9和150.4mg·kg-1,无可见效应浓度(NOECs)分别为50、50、200和100mg·kg-1.鉴于我国土壤类型的多样性十分丰富,不同地区土壤性质差异较大等特点,在制定土壤筛选基准时需考虑污染物在不同土壤中生物有效性与毒性的差异.
Combined acute toxicity of chloride Cd, malathion and acetochlor on Eisenia fetida earthworm
氯化镉、马拉硫磷和乙草胺对赤子爱胜蚓的复合急性毒性

QIAO Wen-Peng,QIAO Yu-Hui,ZHAO Jing,SUN Zhen-Jun,
乔文鹏
,乔玉辉,赵晶,孙振钧

中国生态农业学报 , 2010,
Abstract: 以赤子爱胜蚓(Eisenia fetida)为试验动物, 选取氯化镉、马拉硫磷和乙草胺作为污染物, 通过人工土壤法研究了3种污染物在5种比例两种污染物复合条件下对蚯蚓的急性毒性。研究结果表明, 3种污染物在两两复合污染时的作用类型以拮抗为主, 仅在镉浓度19.46 mg·kg-1和乙草胺175.15 mg·kg-1 (1︰9)复合类型是相加作用, 说明复合污染生态毒理效应与污染物的化学性质和浓度水平均相关。从蚯蚓活体体重减少情况看, 在复合污染条件下, 污染物对蚯蚓生长的影响与单一污染的结果类似, 马拉硫磷对赤子爱胜蚓的影响最大, 乙草胺次之, 而氯化镉的影响最小。
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.