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Role Of Dexamethasone In The Treatment Of Humoral Hypercalcemia Of Malignancy (Hhm) Using Jar Human Choriocarcinoma Cancer Cell Line As A Model
AA Umar Dikko, Hassa A Dikko
Nigerian Journal of Physiological Sciences , 2003,
Abstract: Tumors cause multiple effects on the skeleton and on calcium homeostasis, but they do so in specific patterns which are becoming better defined as the mediators responsible become more fully characterized. Approximately 1,000,000 people die each year in Western Europe and the United States from these three malignancies bone, lung, and breast and the majority of these have bone metastases. Bone is the third commonest site of metastatic disease in tumors of all types and the second most common in breast and prostate cancers. PTH-rP produced by tumor cells of various forms is a killer in at least 15% of the 1,000, 000 cases reported in U.S. and Western Europe a significant number that is hard to ignore. The sole aim of this research is to establish whether dexamethazone inhibit the action of PTH-rP in-vitro and therefore providing a possible remedy for the ailing HHM. Here JAR human choriocarcinoma cells was used with PTH-rP with and without Dexamethasone also, DNA and thymidine incorporation proliferation assays were carried out to determine the extent of stimulation by PTH-rP or inhibition by dexamethasone. The stimulation aggravates HHM, while the inhibition is assumed to alleviate the sufferings of patients with HHM. Dexamethasone was found to inhibit the stimulated cell proliferation by PTH-rP in JAR human choriocarcinoma cells. Thus, the experiment may act as a spring board for alleviating the sufferings and possible treatment of patients with Humoral Hypercalcaemia of Malignancy (HHM)
Influence of IGF-I on adhesion, proliferation, and galectin-1 production in JAr and Jeg-3 choriocarcinoma cell lines
Boji?-Trbojevi? ?anka T.,Jankovi? Miroslava M.,Vi?ovac Ljiljana M.
Archive of Oncology , 2005, DOI: 10.2298/aoo0501007b
Abstract: BACKGROUND: JAr and Jeg-3 choriocarcinoma cell lines are model systems for the transformed trophoblast and allow studies of phenotype and regulatory factors for particular cell functions. Both cell lines express the receptor for insulin-like growth factor-I (IGF-I). Effects of IGF-I on adhesion, proliferation and galectin-1 production in JAr and Jeg-3 cells were studied. METHODS: The effects of IGF-I on proliferation and galectin-1 production were examined by thiazolyl blue assay and cell based solid phase assay using polyclonal anti-galectin-1 antibodies. The cell adhesion assay was performed on Matrigel coated wells. Galectin-1 production and localization was examined by immunocytochemistry. RESULTS: IGF-I decreased adhesion of JAr cells to 70% of the control value (p<0.05). Cell treatment with 10 μg/L of IGF-I significantly increased viable cell number: by 13.5% in JAr and 6% in Jeg-3. Gal-1 was immunolocalized intracellularly and associated with the cell membrane in both cell lines. Production of galectin-1 was significantly increased after treatment with IGF-I compared to control: by 7% in JAr cells and by 16% in Jeg-3 cells (p<0.05). CONCLUSION: The data showed that IGF-I affected adhesion and proliferation of choriocarcinoma cells, depending on the cell line. Both choriocarcinoma cell lines studied here produced galectin-1. The amount of galectin-1 was moderately stimulated by IGF-I.
Mechanisms of matrix metalloproteinase-2 (mmp-2) transcriptional repression by progesterone in jar choriocarcinoma cells
Shlomit Goldman, David H Lovett, Eliezer Shalev
Reproductive Biology and Endocrinology , 2009, DOI: 10.1186/1477-7827-7-41
Abstract: The effect of progesterone on MMP-2 expression in the JAR human choriocarcinoma cell line was analyzed by gelatin zymography. MMP-2 transcript expression was studied using Northern blot and semi-quantitative RT-PCR. Rat promoter deletion analysis, electrophoretic mobility shift and chromatin immuno-precipitation assays were performed in order to locate the DNA binding site and the transcription factors involved in MMP-2 regulation.Progesterone significantly decreased secretion of pro-MMP-2 and MMP-2 transcript expression level in a dose-dependent manner. Progesterone (1 microM) significantly decreased both human and rat MMP-2 promoter activity (80.1% +/- 0.3 and 81.3% +/- 0.23, respectively). Progesterone acts through the SP1 family transcription factors-binding site, located between -1433 and -1342 bp region from the transcriptional start site of the rat MMP-2 promoter, which are present in the orthologous human MMP-2 promoter. Progesterone receptor (PR), SP2, SP3 and SP4 proteins are constitutively bound to this consensus sequence.Progesterone reducesPR and SP4 binding to the MMP-2 promoter, thereby suppressing transcription. Progesterone also promotes SP4 degradation. These novel mechanisms of MMP-2 regulation by progesterone provide the biological rationale for the use of progesterone in clinical settings associated with increased MMP-2 expression.Matrix metalloproteinase-2 (MMP-2) plays a critical role in invasion, metastasis, angiogenesis and tissue remodelling [1]. The protein is widely expressed by a number of normal and transformed cells [2]. Recent studies have highlighted the important role of MMP-2 in the invasive potential of metastatic endometrial [3,4], ovarian [5] and trophoblast cells [6], as well as invasion of the normal trophoblast [7]. The ability of trophoblasts to infiltrate the uterine wall and to anchor the placenta to it, as well as their ability to infiltrate and adjust utero-placental vessels to pregnancy, is dependent upon MMP-2 secretio
Matrix metalloproteinase-2 and -9 secretion by the human JAR choriocarcinoma cell line is stimulated by TNF-α  [PDF]
Beno?t Chénais
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.31009
Abstract: The JAR choriocarcinoma cell line share many of the characteristics of early placental trophoblast cells including the invasion properties. Matrix metallo-proteinases (MMPs), the main actors of matrix proteolysis, are involved in normal invasion as well as in the invasive character of tumor cells and the metastase formation. Tumor necrosis factor-α (TNF-α) is present in the placental environment and TNF-α levels are elevated in some placental pathologies. In the present work, we addressed whether TNF-α is a modulator of JAR cell MMP secretion. Following TNF-α stimulation, zymographic analysis showed the increased secretion of the active form of MMP-2 and to a lesser extent proMMP-2 and MMP-9. In addition, MMP-2 gene expression only increased slightly whereas MMP-9 and TIMP-1 transcripts were undetectable. This suggests that TNF-α may modulate the secretion of MMPs independently of MMP gene expression control.
Choriocarcinoma cell line Response to Dexamethasone
anka Boji -Trbojevi , Nikola Kolund i , Milo Petronijevi , Ljiljana Vi ovac
Journal of Medical Biochemistry , 2010, DOI: 10.2478/v10011-010-0017-8
Abstract: Choriocarcinoma cell lines JAr and JEG-3 are model systems for the study of transformed trophoblast. Both cell lines were shown to produce galectin-1, expression of which was increased in choriocarcinoma when compared to the normal trophoblast of pregnancy. In this study the effects of synthetic glucocorticoid dexametha-sone were investigated in both JAr and JEG-3 cell lines by the MTT test, cell based ELISA, and the cell adhesion and migration tests. Viable cell number/cell proliferation of JAr cells was significantly increased after treatment with 0.1 and 1 nmol/L of dexamethasone, while proliferation of JEG-3 cells was significantly increased after treatment in the whole concentration range of dexamethasone (0.1-100 nmol/L). Galectin-1 in JAr cells was modulated by dexamethasone, which mildly, but significantly decreased production at low concentrations (0.1 and 1 nmol/L). In JEG-3 cells production of galectin-1 was significantly decreased only after treatment with 100 nmol/L of dexamethasone. Cell adhesion of JEG-3 was significantly increased in the presence of lactose, an inhibitory sugar for gal-1, while dexamethasone induced decrease of JEG-3 cell migration. These findings have shown that dexamet-hasone may affect proliferation, gal-1 production and cell migration, in a cell line specific manner. These data suggest that glucocorticoid treatment in vivo might have the potential to affect cell functions in choriocarcinoma.
Epigenetic Therapy in Human Choriocarcinoma  [PDF]
Noriyuki Takai,Hisashi Narahara
Cancers , 2010, DOI: 10.3390/cancers2031683
Abstract: Because epigenetic alterations are believed to be involved in the repression of tumor suppressor genes and promotion of tumorigenesis in choriocarcinomas, novel compounds endowed with a histone deacetylase (HDAC) inhibitory activity are an attractive therapeutic approach. HDAC inhibitors (HDACIs) were able to mediate inhibition of cell growth, cell cycle arrest, apoptosis, and the expression of genes related to the malignant phenotype in choriocarcinoma cell lines. In this review, we discuss the biologic and therapeutic effects of HDACIs in treating choriocarcinoma, with a special focus on preclinical studies.
A pure nongestational choriocarcinoma of the ovary  [cached]
Mohammad Ali Roghaei,Parvin Mahzuni,Faryma Rezaei
Journal of Research in Medical Sciences , 2007,
Abstract: Choriocarcinoma arises in the ovary from gestational or nongestational origin. Nongestational choriocarcinoma of the ovary is extremely rare and the pure type is less frequent than the mixed type with other germ cell tumors. We report a pure nongestational choriocarcinoma primarily arising in a 47-year-old woman’s ovary. Following abdominal operative procedure, careful examination of the tumor revealed choriocarcinoma without combination of other germ cell tumors. Multiple courses of the chemotherapy with and EMA/CO regimen were effective for this case. KEY WORDS: Choriocarcinoma, nongestational, ovary.
固体氧化物燃料电池Cu-LSCM-CeO2/LSCM-YSZ/Ni-ScSZ复合阳极制备及性能  [PDF]
电化学 , 2014, DOI: 10.13208/j.electrochem.131129
Abstract: 采用流延法制得LSCM-YSZ阳极支撑层/Ni-ScSZ阳极活性层/ScSZ电解质层复合膜,在LSCM-YSZ支撑层上印刷一层Cu-LSCM-CeO2阳极催化层,即Cu-LSCM-CeO2/LSCM-YSZ/Ni-ScSZ功能梯度层阳极.研究表明,Cu/LSCM/CeO2质量配比为2:7:1功能梯度阳极(LSCM-YSZ2010)有较好的性能,单电池以氢气和乙醇为燃料(750oC)最大功率密度分别为511和390mW?cm-2,单电池稳定性实验表明,LSCM-YSZ2010阳极单电池以乙醇为燃料750oC长时间运行218h,性能稳定.X-射线能量散射分析表明该阳极具有较好的抗碳沉积性能.
Unusual presentation of choriocarcinoma
PG Balagopal, Manoj Pandey, K Chandramohan, Thara Somanathan, Ashwin Kumar
World Journal of Surgical Oncology , 2003, DOI: 10.1186/1477-7819-1-4
Abstract: A case of malignant trophoblastic disease with brain metastasis, raised intra cranial pressure and small bowel metastasis presenting with acute abdomen is reported.Malignant transformation in a hydatidiform mole is rare event. Involvement of gastrointestinal tract is rarer even in presence of disseminated disease. Surgery is the treatment of choice for gastrointestinal complications.Trophoblastic diseases comprise a variety of biologically interrelated conditions which form a clinical spectrum consisting of four distinct clinical pathological entities like (1) molar pregnancy (2) invasive mole (3) placental site trophoblastic tumours and (4) choriocarcinoma [1].Incidence of hydatidiform mole ranges from 1 in 500 in India to 1 in 3000 in USA. Like wise malignant potential of the disease is also higher in Southeast Asia reaching as high as 10–15% compared to 2–4% in west. Some of the hydatidiform moles erode the wall of uterus, burrow into myometrium and may even burst though the uterus into peritoneum and are called invasive mole. Even though locally aggressive, invasive mole does not metastasise. On the other hand choriocarcinoma is rare but one of the most malignant neoplasms arising in the body of uterus. choriocarcinoma metastasises readily. It is not uncommon to find secondary nodules in the cervix or vagina. The lungs, brain and liver are the distant organs more often involved. Lymph nodes are rarely the site of metastasis [2]. We report here a case of hydatidiform mole metastasising to brain and later presenting with intestinal metastasis and perforation.A twenty-four-year old housewife underwent dilatation and curettage for vaginal bleeding in 1994. Pathological examination of curettage specimen showed hydatidiform mole. She later conceived and had two normal deliveries. She remained asymptomatic for eight years. In January 2002 she was brought to medical causality with features suggestive of raised intra cranial pressure. A computerised tomographic scan of t
Progress of the Diagnosis and Treatment of Pulmonary Metastasis of Gestational Choriocarcinoma  [cached]
Dongjie MA,Zhiyong ZHANG,Shanqing LI
Chinese Journal of Lung Cancer , 2011, DOI: 10.3779/j.issn.1009-3419.2011.10.06
Abstract: Gestational choriocarcinoma is the most common gestational trophoblastic neoplasia. It is prone to hematogenous metastasis, most commonly to the lungs. With the advent and development of chemotherapy, choriocarcinoma has become a curable tumor. However, patients with drug-resistant and recurrent choriocarcinoma are difficult to treat, even with the management of pulmonary metastasis. Resorting to surgery is also a tough decision given the challenges of identifying the appropriate surgical indication and timing. This review discusses the basic principles of management as well as recent advances in the diagnosis and treatment of patients with pulmonary metastasis of gestational choriocarcinoma.
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