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Evolution of chemical-physical parameters and rheological characteristics of Sarda and Maltese goat dry hams  [cached]
Rina Mazzette,Domenico Meloni,Rita Melillo,Simonetta Gianna Consolati
Italian Journal of Food Safety , 2012, DOI: 10.4081/ijfs.2012.5.17
Abstract: In Sardinia, goat farming is a very important resource. Sarda and Maltese breed are reared mainly for milk production and for suckling kids meat, while meat from adult goats is undervalued. The use of adult goat meat to obtain ripened ham will contribute to safeguard the Sardinian goat supply chain. The aim of the present study was to characterize Sarda and Maltese goat dry ham in order to evaluate the quality of autochthonous goat breed meat. Chemical-physical characteristics were determined dur-ing the production stages, while the rheological and colour parameters and the composition of the goat ham were determined at the end of ripening. The pH evolution during processing were similar to other cured meat products, e.g. sheep hams, even though the values were high, especially in the products from Sarda breed. The aw value regularly decreased during processing. Colour parameters (L*, a*, b*) in the hams from Maltese goat breed were significantly (P<0.05) higher than in those from Sarda. The Sarda goat ham showed a significantly lower percentage of moisture (42% vs 52%), an higher protein content (44.35% vs 34.19%), while no differences were pointed out in the total fat content. Among the ham rheological properties, hardness parameters showed higher levels (13850.22±7589.92 vs 11073.99±6481.31, respectively in Sarda and Maltese hams) in comparison to similar products from pork and sheep, while adhesiveness value was lower. The results show that the quality parameters of goat ripened hams are affected mainly by the charac-teristics of the goat meat, in relation on the breed and the breeding system, and, less, by the traditional technology.
Comparison of G k eada, Maltese and Turkish Saanen Goat Genotypes for Reproduction Traits  [PDF]
C.Tolu,T. Savas
Journal of Tekirdag Agricultural Faculty , 2010,
Abstract: This study aimed at comparative investigation of estrus, pregnancy, mating performance, behaviors, and kid yield at birth in different goat genotypes. The study was carried out at the Technological and Agricultural Research Centre of anakkale Onsekiz Mart University for a period of two years. Although the courtship behavior index of females did not differ, the bucks of G k eada had lower courtship behavior index and jumping frequency with ejaculation than did other genotypes (P≤0.05). Maltese bucks displayed courtship behavior index and mounting frequency with ejaculation similar to Turkish Saanen bucks, but had lower mounting frequency without ejaculation (P≤0.05). First estrus and birth rates (buck under) were 33% and 25% for G k eada, 65% and 42% for Maltese and 96% and 64% for Turkish Saanen, respectively (P<0.0001). The period between buck introduction and first estrus or conception increased in all genotypes in the second year of the study. G k eada had longer periods and lower return rates in both years (P≤0.05). Kid yield per goat at birth in the second year increased significantly in G k eada and Maltese genotypes and was found as Maltese (1.9; 2.4), Turkish Saanen (1.9; 1.8) and G k eada (1.6; 1.8) in the descending order.
RAPD Analysis on Guizhou Native Goat Breeds
贵州地方山羊品种的RAPD分析

CHEN Xiang,LIAO Zheng lu,LI Guo hong,Zhang Yun,JIAN Cheng song,WEI Hong,LI Hong,
陈祥
,廖正录,李国红,张芸,简承松,魏泓,李虹

动物学研究 , 2004,
Abstract: We used 180 primers in RAPD analysis of Guizhou native goats (the small xiang goat,Guizhou white goat,Guizhou black goat and Qianbei pockmarked goat),Nanjiang brown goats and Boer goats.Of the primers,27 amplified polymorphic patterns,including 281 bands.Of the bands,115 were polymorphic and their frequency was 40 92% on average (range 20%-80%).Each of the 27 primers had 10 41 bands on average (range 4-16),and the fragment length of products was 210-2?800?bp.Genetic distance index appeared the lowest between the Guizhou black goat and Guizhou white goat (0 0605),and the highest between the Boer goat and the other goats (0 1059-0 1488).NJ tree indicated that the Guizhou white goat was closely related to the Guizhou black goat,and the next was the Qianbei pockmarked goat;the small xiang goat was farther from the other Guizhou native goats than Nanjiang brown goat from.The results analysis suggest that the small xiang goat is a independent breed on genetics,and Guizhou native goat breeds have the close genetic relationship,low genetic variability and high genetic stability.
Genetic Diversity among Six Breeds of Indian Goat Using RAPD Markers  [PDF]
Anita Yadav,B.R Yadav
Biotechnology , 2007,
Abstract: The genetic variations were studied by RAPD technology among six breeds of Indian goats viz. Barbari, Black Bengal, Jamnapari, Marwari, Sirohi and Jhakrana. Out of 40 primers screened using DNA samples of six goat breeds, only 10 primers generated reproducible and distinct RAPD profile. Only distinct and prominent bands were scored. Estimates of between breed’s similarity as indicated by genetic identity and genetic distance ranged between 0.7156 to 0.9506 and 0.0506 to 0.3347, respectively. From dendrogram, it has been observed that Marwari- Sirohi breeds have closer relationship forming a distinct cluster whereas Black Bengal and Barbari were distinctly different from each other.
Genetic polymorphisms of αS1-, αS2- and k-casein in Maltese goat breed  [cached]
S. Chessa,G. Ceriotti,C. Dario,G. Erhardt
Italian Journal of Animal Science , 2011, DOI: 10.4081/ijas.2003.s1.58
Abstract: The casein genes are organized as a tight cluster including αS1- (CSN1S1), β- (CSN2), αS2- (CSN1S2) and k-casein (CSN3). Investigation at casein haplotype level is therefore necessary to detect effects which could be used for the genetic improvement of goat breeds aiming to preserve biodiversity, safeguard typical products, and valorise nutritional properties of goat milk. Mutations responsible for the reduced or null expression of CSN1S1........
Genetic characterization of the Moxotó goat breed using RAPD markers
Oliveira, Rejane Rodrigues de;Egito, Andréa Alves do;Ribeiro, Maria Norma;Paiva, Samuel Rezende;Albuquerque, Maria do Socorro Maués;Castro, Silvia Ribeiro;Mariante, Arthur da Silva;Adri?o, Manuel;
Pesquisa Agropecuária Brasileira , 2005, DOI: 10.1590/S0100-204X2005000300006
Abstract: the objective of this study was to verify the genetic diversity between and within seven populations of moxotó goat (n = 264) from the states of pernambuco, paraíba and rio grande do norte, using rapd (random amplified polymorphic dna). moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the northeast region of brazil. the genetic characterization of these genetic resources is essential to conservation and breeding programs. dna was extracted from lymphocytes using a non-organic protocol. the 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. the analysis of molecular variance (amova) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. the analysis of variance among the pairs of populations demonstrated that the populations located in floresta, pe x angicos, rn presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. nei's genetic distances varied between 0.0546 and 0.1868 in the populations. the dendrogram generated showed that the canindé breed, used as outgroup, clustered with the populations of moxotó, indicating a possible common origin of the naturalized goat breeds.
Studies of Random Amplified Polymorphic DNA(RAPD)of Different Goat Populations Crossbred by Boer Goat
波尔山羊杂交后代及其亲本随机扩增多态DNA研究

LI Xiang-long,TIAN Qing-yi,MA Guo-qiang,LIU Jin-fu,FENG Min-shan,NIU Yi-bing,SUN Nai-quan,MA Guang-xing,GUO Run-min,
李祥龙
,田庆义,马国强,刘金福,冯敏山,牛一兵,孙乃权,马广星,郭润民

遗传 , 2000,
Abstract: The random amplified polymorphic DNA(RAPD)of 128 individuals was studied,which were from Boer goat, Tangshan dairy goat,Qinglong native goat and their hybrids crossbred with Boer goat.The average index of genetic polymorphism for whole population(Hsp)and the index of genetic differentiation were 0.6974 and 0.9706,respectively.The average index of genetic distance between populations(0.1314~0.2052)was significantly higher than that within populations(0.0582~0.1440).All of these indicated that the genetic polymorphism was not only abundant,but also the genetic variation was mainly existed between goat populations.The molecular dendrogram among goat populations was in accord with their genetic relationship.
Genetic characterization of two Sudanese goat breeds (Capra hircus) using RAPD molecular markers
E El Gaali, M Satti
African Journal of Biotechnology , 2009,
Abstract: Seven primers of randomly amplified polymorphic DNA (RAPD) were selected to study the genetic variations among 14 individuals of goat (Capra hircus) from two domestic Sudanese goat breeds (Nilotic and Nubian). The test generated 59 entirely repeatable RAPD fragment bands and the statistical analysis showed 55 polymorphic bands among the 14 individuals. The genetic distances among the population range from 8 to 72%. The highest dissimilarity coefficient was between individuals within the Nilotic breeds while there was a comparatively low degree of differentiation among the Nubian population. The constructed UPGMA dendrogram of the coefficient of similarity showed that the Nubian clustered together while the individuals from the Nilotic form 4 groups. It was clearly seen that the link between the individual of the Nilotic is quite weak and some of them linked to the Nubian. The results of the study offer useful information about some Sudanese goat breeds.
Identification of Genomic Markers by RAPD-PCR Primer in Leukemia Patients  [PDF]
N. Saleh,M.A. Ibrahim,E. Archoukieh,A. Makkiya
Biotechnology , 2010,
Abstract: The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia.
Genomic DNA Extraction and RAPD Analysis for Gastrodia elata Bl. (Orchidaceae) Using an Improved Method  [PDF]
Huailin Xiong,Bo Luo,Yujie Zheng,Yuanyuan Cui
Journal of Biological Sciences , 2012,
Abstract: One improved randomly amplified polymorphic DNA (RAPD) technique has been used in animal phylogenetic relationships and fingerprints analysis by prolonging the ramp time. However, little was known whether the PCR ramp time and G/C content of primers effect on RAPD analysis of medicinal Gastrodia elata Bl. plants. The present study was conducted with the objectives to extract genomic DNA of G. elata, execute RAPD analysis with different PCR ramp time and discuss the relationship between the amplification efficiency and the G/C content of RAPD primers subsequently. The Cetyltrimethylammonium Bromide (CTAB) protocol was used in the genomic DNA extraction. Ten RAPD primers were randomly selected in PCR amplifications. The ramp time parameters from annealing to extension were used 0.3 and 3°C sec-1, respectively. The concentration of G. elata genomic DNA were about 50 ng μL-1 with a 1.93 purity. Obviously, the amplified band numbers and resolution were improved when using a 0.3°C sec-1 ramp time in the RAPD analysis. The band number increase is closely related to the G/(G+C) ratio of RAPD primers. Therefore, the extracted G. elata genomic DNA is suitable for PCR amplifications and the prolonged ramp time is helpful to improve the RAPD resolution and production. These are valuable references for molecular identification and biodiversity analysis of G. elata populations.
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